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Search Results: 1 - 10 of 177043 matches for " Eveline Queiroz de Pinho Tavares "
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An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues
Eveline Queiroz de Pinho Tavares,Marciano Regis Rubini,Thiago Machado Mello-de-Sousa,Gilvan Caetano Duarte,Fabrícia Paula de Faria,Edivaldo Ximenes Ferreira Filho,Cynthia Maria Kyaw,Ildinete Silva-Pereira,Marcio Jose Po?as-Fonseca
Enzyme Research , 2013, DOI: 10.1155/2013/287343
Abstract: Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35?kDa protein which was purified through a two-step chromatography procedure. The highest enzyme activity was detected at C/pH 4. rEG A retained 100% of activity when incubated at 45 and C for 72?h. Purified rEG A kinetic parameters towards CMC were determined as ?mg/mL, ?mmol/min, and 55.8?IU (international units)/mg specific activity. Recombinant P. pastoris supernatant presented hydrolytic activity towards lignocellulosic residues such as banana stalk, sugarcane bagasse, soybean residues, and corn straw. These data indicate that rEG A is suitable for plant biomass conversion into products of commercial importance, such as second-generation fuel ethanol. 1. Introduction One of the major challenges of modern society is to promote economic growth in a sustainable model. Global demands of energy consumption stimulate the research on alternative fuels, aiming the reduction of the dependence on nonrenewable energy sources. For some decades now, Brazil and the USA have successfully produced bioethanol from sugarcane and corn, respectively. Nonetheless, plant biomass generated by extensive cultures, and which is not totally converted into useful by-products such as fertilizers and animal feed, tends to accumulate and cause environmental problems. Numerous efforts have been made in order to develop biotechnological routes to produce the so-called second-generation bioethanol from agriculture residues such as corn stover, rice straw, sorghum bagasse, corncobs, wheat bran, wheat straw, and sugarcane bagasse. The limiting step of this process is the availability of low-cost efficient enzymes to convert lignocellulose into fermentable glucose units. Filamentous fungi can produce and secrete enzymes which efficiently degrade cellulose, a linear polymer of glucopyranose units connected by β-1,4 bonds, to oligosaccharides and glucose. Based on model organisms from the genera Trichoderma and Phanerochaete, fungi cellulolytic enzymes acting in synergism have been classified as (1) endoglucanases or endo-β-1,4-glucanases (EC 3.2.1.4), responsible for the random attack of internal glycosidic bonds of the cellulose amorphous region, generating oligosaccharides of various sizes and new chain ends
Larvicidal Activity of essential oils from Brazilian plants against Aedes aegypti L.
Cavalcanti, Eveline Solon Barreira;Morais, Selene Maia de;Lima, Michele Ashley A;Santana, Eddie William Pinho;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000500015
Abstract: aedes aegypti l. is the major vector of dengue fever, an endemic disease in brazil. in an effort to find effective and affordable ways to control this mosquito, the larvicidal activities of essential oils from nine plants widely found in the northeast of brazil were analyzed by measurement of their lc50. the essential oils were extracted by steam distillation and their chemical composition determined by gl-chromatography coupled to mass spectroscopy. the essential oils from cymbopogon citratus and lippia sidoides, reported in the literature to have larvicidal properties against a. aegypti, were used for activity comparison. the results show that ocimum americanum and ocimum gratissimum have lc50 of 67 ppm and 60 ppm respectively, compared to 63 ppm for l. sidoides and 69 ppm for c. citratus. these results suggest a potential utilization of the essential oil of these two ocimum species for the control of a. aegypti.
Multicenter Brazilian Study of Oral Candida Species Isolated from Aids Patients
Sant'Ana Priscilla de Laet,Milan Eveline Pípolo,Martinez Roberto,Queiroz-Telles Flávio
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: Oropharyngeal candidiasis continues to be considered the most common opportunistic disease in Aids patients. This study was designed to investigate species distribution, serotype and antifungal susceptibility profile among Candida spp. isolated from the oral cavity of Aids patients recruited from six Brazilian university centers. Oral swabs from 130 Aids patients were plated onto CHROMagar Candida medium and 142 isolates were recovered. Yeast isolates were identified by classical methods and serotyped using the Candida Check system-Iatron. Antifungal susceptibility testing was performed according to the NCCLS microbroth assay. C. albicans was the most frequently isolated species (91%), and 70% of the isolates belonged to serotype A. We detected 12 episodes of co-infection (9%), including co-infection with both serotypes of C. albicans. Non-albicans species were isolated from 12 episodes, 50% of them exhibited DDS or resistance to azoles. Otherwise, only 8 out 130 isolates of C. albicans exhibited DDS or resistance to azoles. Brazilian Aids patients are infected mainly by C. albicans serotype A, most of them susceptible to all antifungal drugs.
Educa??o em saúde nas páginas da REBEn no perído de 1995 a 2005
Carvalho, Viviane Lemes da Silva;Clementino, Viviane de Queiroz;Pinho, Lícia Maria de Oliveira;
Revista Brasileira de Enfermagem , 2008, DOI: 10.1590/S0034-71672008000200016
Abstract: during our graduation, we live deeply many practical of education in health that motivated us to carry through this study. the objectives are to describe practical educative developed by nurses; identify the people, the place and describe the results gotten with the educative practical. study of the systemize bibliographical type. the information had been collected through the election and analysis, of articles on education in health, published in the brazilian magazine of nursing (reben), in the period of 1995 to 2005. after the analysis, 38 articles had been selected and grouped. we could observe that the educative practical happen more frequently at the hospital, the used strategies have been very creative, they enclose users of all ages and the nurses are the ones that more develop educative practices.
Degrada??o eletroquímica da vinha?a usando eletrodo de diamante dopado com boro
Batista, Eveline Cristine;Oliveira, Robson Tadeu Soares de;Ferreira, Rafael de Queiroz;Miwa, Douglas;Santos, Mauro Coelho dos;
Química Nova , 2011, DOI: 10.1590/S0100-40422011000900007
Abstract: the degradation of vinasses in aqueous solution from ethanol industry has been investigated by electrochemical oxidation using a boron doped diamond electrode (bdd). samples of vinasses were electrolyzed in medium of (0.1 mol l-1) na2so4 solutions at controlled potentials of +2.4, +3.0 and +4.0 v (vs. ag/agcl) and exhibited considerable reduction of total organic carbon. the cyclic voltammetry studies indicate that the vinasses are oxidized irreversibly over the bdd at 2.0 v (vs. ag/agcl) in diffusion controlled process. from the experimental results it is clear that the bdd electrode can be a valuable tool to the electrochemical degradation of vinasses in practical applications
Rhino-orbito-cerebral mucormycosis Zigomicose rino-órbito-cerebral
Igor Teixeira Raymundo,Beatriz Gonzalez De Araújo,Carina De Carvalho Costa,Joana Pinho Tavares
Brazilian Journal of Otorhinolaryngology , 2009, DOI: 10.1590/s1808-86942009000400024
Abstract:
Correlation between smoking and anthropometric variables in blood donors in Piauí
Júlio César Queiroz de Fran?a,Elson do Nascimento Oliveira,Eveline Brand?o Madeira,Fabiano Loiola Santos
Revista Brasileira em Promo??o da Saúde , 2010,
Abstract: Objective: To assess the relationship between smoking, body weight and body fat distribution of blood donors in Piaui. Methods: A cross-sectional study with a sample of 595 blood donors, regardless of sex, from 18-61 years, registered at the Center for Hematology of Piauí (HEMOPI). Data were collected from November/2008 to February/2009. Cigarette consumption was assessed through an informed questionnaire. The measurements taken were: weight, height, waist and hips. We assessed the body mass index (BMI), waist circumference (WC) and waist-hip ratio (WHR). The group was divided into three subgroups (smokers, nonsmokers and former smokers) by the presence or absence of smoking practice. The association between variables was assessed by applying the Mann-Whitney/Wilcoxon test adopting the value of p 0.05 for significance. Results: Waist circumference and waisthip ratio were found positively associated only in females, both to current smoking practice (p = 0.0196 and p = 0.0050, respectively) as to the former (p = 0.0167 and p = 0.0167, respectively). Among smokers, the quantity of cigarettes smoked per day was positively associated with BMI in males (p = 0.0179) and WHR in females (p = 0.0448). Conclusion: Cigarette smoking, particularly among females, was associated with body fat distribution
Determina??o de propriedades térmicas de gr?os de milho
Andrade, Ednilton Tavares de;Couto, Sandra Maria;Queiroz, Daniel Mar?al de;Peixoto, Abra?o Brito;
Ciência e Agrotecnologia , 2004, DOI: 10.1590/S1413-70542004000300002
Abstract: values for bulk density, thermal conductivity, thermal diffusivity and specific heat of corn grains were obtained in this work as a function of product moisture content. the infinite cylinder method with a central heat source was used in the thermal conductivity and diffusivity determinations. the equipment used consisted of an aluminum tube (0.1m of diameter and 0.5m of height) isolated in the superior and inferior extremities. a resistor (nickel-chrome; strength of 4.16 w.m-1; diameter of 0.0006438m) was used as a heat source that was located at the center of the cylinder (longitudinal direction). during each determination, the resistor was submitted to a difference of potential of 2.08v that provided an electric current of 1a. the moisture content of the tested samples varied from 8.6 to 17.1% w.b. the behavior of the values of thermal conductivity, diffusivity and specific heat as a function of the product moisture content can be represented by linear relationships. the thermal conductivity and specific heat of the grains increase with the product moisture content, while the thermal diffusivity decreases. errors involved in the thermal properties determinations are also discussed in this work.
Evaluation of pesticide adsorption in gas chromatographic injector and column
Pinho, Gevany Paulino de;Silvério, Flaviano Oliveira;Neves, Ant?nio Augusto;Queiroz, Maria Eliana Lopes Ribeiro de;
Química Nova , 2012, DOI: 10.1590/S0100-40422012000400016
Abstract: components in complex matrices can cause variations in chromatographic response during analysis of pesticides by gas chromatography. these variations are related to the competition between analytes and matrix components for adsorption sites in the chromatographic system. the capacity of the pesticides chlorpyrifos and deltamethrin to be adsorbed in the injector and chromatographic column was evaluated by constructing three isotherms and changing the column heating rate to 10 and 30 oc min-1. by using ancova to compare the slope of calibration graphs, results showed that the higher the injector temperature (310 oc) the lower the pesticide adsorption. also, deltamethrin influenced the adsorption of chlorpyrifos on the column chromatographic.
Bioestimulante e fertilizante associados ao tratamento de sementes de milho
Ferreira, Leidiane Aparecida;Oliveira, Jo?o Almir;Von Pinho, édila Vilela de Resende;Queiroz, Danilo Luiz de;
Revista Brasileira de Sementes , 2007, DOI: 10.1590/S0101-31222007000200011
Abstract: the corn crop is very technological and this productive system absorbs all possible innovations to improve the yield, nevertheless some care should be taken concerning the addition of new chemicals to the seeds. the objective of this study was to evaluate the effect of seed treatment, with the biostimulant stimulate? as well with the liquid fertilizer cellerate? at 6 months before sowing and in the pre- sowing, on the physiological quality of seeds, on corn yield. experiments were performed at the federal university of lavras, mg, brazil in 2005, at field and laboratory level with a corn hybrid (gnz 2004) and with a corn line (l57). the treatments used for corn assays were: witness (without chemicals); 5 ml.kg-1 stimulate?; 5 and 10 ml.kg-1 cellerate?. the seed physiological quality was accessed by germination, evi, cold test and above and under ground dry mass of plantlets and by the analysis of the enzymes esterase, superoxide dismutase, malate dehydrogenase, alcohol dehydrogenase, catalase and á-amylase. the laboratory experiments were carried out in a complete randomized design with four replications. the a randomized block design was used of hybrid and line corn field with four replicates in a 4 x 2 factorial with the time and the compounds for seed treatment being the factors. in this assay plant and spike height, number of spikes per plant and productivity were evaluated. corn seeds treated with stimulate? and cellerate? promoted greater plantlet development in the line and of roots in the hybrid. a high concentration of cellerate? seems to reduce hybrid emergence and germination as well as the emergence of the line seeds when they have been treated at the pre-sowing stage. an increase was observed in the activity of the enzymes malato dehydrogenase and catalase in the hybrid seeds treated with the high concentration of cellerate? in the pre-sowing and 6 months before sowing, respectively. however, the line seeds when treated with high concentrations of celle
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