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Search Results: 1 - 10 of 485119 matches for " Eva; Martínez Antu?a "
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Frecuencia de la leucemia promielocítica en Cuba Frequency of promyelocytic leukemia in Cuba
María Teresa Milanés Roldán,Porfirio Hernández Ramírez,Eva Svarch Guerchicoff,Gisela Martínez Antua
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2001,
Abstract: Se realizó el análisis de 1 112 pacientes con LA (leucemias agudas) de todo el país (714 adultos y 398 ni os menores de 15 a os de edad), 637 (554 adultos y 83 ni os) presentaron LMA (leucemia mieloide aguda), y se diagnosticaron 98 casos (72 adultos y 26 ni os) con LPM (leucemia promielocítica aguda). La LPM representó el 8,9 % de todos los casos de LA en adultos y el 6,5 % de todos los casos de LA en ni os. Su frecuencia en relación con el total de casos de LMA sin límite de edad representó el 15,3 %. Sin embargo, cuando esta frecuencia se analizó por grupos de edades se apreció que en los ni os era de 31,3 % mientras que en los adultos fue solo del 13 %. En nuestro estudio se pudo comprobar que de forma similar a lo que ocurre en ciertas regiones geográficas y grupos poblacionales, existe un incremento de la frecuencia de LPM en ni os en relación con los datos de la literatura previa. Nuestros resultados destacan la necesidad de considerar por separado este tipo de leucemia en ni os y adultos, pues su análisis en un solo grupo podría ocultar variaciones en su frecuencia 1 112 patients with AL (acute leukemias) from all over the country (714 adults and 398 children under 15) were analyzed. 637 (554 adults and 83 children) had AML (acute myeloid leukemia) and 98 cases (72 adults and 26 children) were diagnosed APL (acute promyelocytic leukemia). The APL accounted for 8.9 % of all the cases of AL in adults and 6.5 % of all the cases of AL in children. Its frequency in relation to the total of cases of AML with no age limit represented 15.3 %. However, when this frequency was analyzed by age group, it was observed that it was 31.3 % in children, whereas in adults it was just 13 %. In our study, it was possible to prove that similarly to what happens in certain geographic regions and population groups, there is an increase of the frequency of APL in children compared with the data found in the previous literature. Our results stress the need to consider by separate this type of leukemia in children and adults, since its analysis in only one group may occult variations in its frequency
Estudio del reordenamiento molecular de los genes TEL/AML1 en la leucemia linfocítica aguda: Resultados preliminares
Cayado Gutiérrez,Niubys; Mu?iz Fernández,Adriana; González Otero,Alejandro; Svarch,Eva; Martínez Antua,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: acute lymphocytic leukemia (all) represents approximately 80% of the pediatric leukemias.the existance of a cryptic translocation, the t (12;21) (p12;q22) in the type b all, which is not detected by the conventional cytogenetic techniques and involve the tel and aml1 oncogenes, has been recently shown by molecular biology. this alteration is at present the most common in this leukemia and it is observed approximately in 25% of the cases. some authors have stated that such translocation identifies a group of patients with a very favorable evolution and that's why it is considered as an indicator of good prognosis. the determination of the t (12;21) in the study of all has a prognostic importance and it also serves as a marker for the detection of the minimal residual disease. in our paper, we standardized the rt-pcr technique for the detection of the t (12;21) and we also analized samples from 20 pediatric patients with type b all, which at the time of the study was in the phase of initial diagnosis or on relapse. in the study, 5 of the 20 patients showed rearranged tel/aml l genes, which accounted for 25% of the cases. this frequency agrees with what is reported in literature up to now
Frecuencia de la leucemia promielocítica en Cuba
Milanés Roldán,María Teresa; Hernández Ramírez,Porfirio; Svarch Guerchicoff,Eva; Martínez Antua,Gisela; Ballester Santovenia,José M;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2001,
Abstract: 1 112 patients with al (acute leukemias) from all over the country (714 adults and 398 children under 15) were analyzed. 637 (554 adults and 83 children) had aml (acute myeloid leukemia) and 98 cases (72 adults and 26 children) were diagnosed apl (acute promyelocytic leukemia). the apl accounted for 8.9 % of all the cases of al in adults and 6.5 % of all the cases of al in children. its frequency in relation to the total of cases of aml with no age limit represented 15.3 %. however, when this frequency was analyzed by age group, it was observed that it was 31.3 % in children, whereas in adults it was just 13 %. in our study, it was possible to prove that similarly to what happens in certain geographic regions and population groups, there is an increase of the frequency of apl in children compared with the data found in the previous literature. our results stress the need to consider by separate this type of leukemia in children and adults, since its analysis in only one group may occult variations in its frequency
Bases moleculares de las leucemias agudas
G. Martínez Antua
Iatreia , 2006,
Abstract: El gran desarrollo de la biología molecular en los últimos a os ha contribuido a un importante avance en los conocimientos relacionados con las bases moleculares de las leucemias agudas (LA). Ademas de profundizar en la biología de estas enfermedades y conocer las bases moleculares, ha renido también gran impacto en mejorar el resultado de los tratamientos y disminuir la toxicidad de las terapias.
Aportes al estudio de la drepanocitosis: Análisis clínico y hematológico en los primeros 5 a?os de la vida
García Peralta,Tania; Nordet Carrera,Ileana; Machín García,Sergio; González Otero,Alejandro; Mu?iz Fernández,Adriana; Martínez Antua,Gisela; Wade Mateo,Maura; Svarch,Eva;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 1999,
Abstract: 104 patients under 6 were studied. in 93,1 % of them the diagnosis was made before the first year of life, whereas in 47.6 % it was prenatal. the median of follow-up was 31 months (1-108). the highest incidence of clinical events in sickle cell anemia (sca) occurred between 1 and 3 years old. 91.6 % of the infections were pulmonary and 21.7 % of the respiratory episodes were considered as acute chest syndrome. the events in hemoglobinopathy sc (hsc) were less frequent than in sickle cell anemia. only one patient died in a period of almost 10 years since the study began. hemoglobin and fetal hemoglobin (hbf) were higher among females with sca. the commonest haplotypes of the block of genes b were benin and bantu. it was observed a frequency of thalassemia of 23.8 % in sickle cell anemia and of 23.7 % in hsc. bantu and non-bantu haplotypes and a thalassemia were not associated with the clinical manifestations or with the hematological parameters. the systematic follow-up of the patients since the first years of life reduces mortality in this period
Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea
Amor Vigil,Ana M; Martínez Antua,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. the present paper updates the concepts of chimerism, its classification and the different ways to determine it. the most recommended is the highly polymorphic area amplification in the dna using the polymerase chain reaction technique. this technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. it has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. finally, the paper presents the recommendations of the american society of blood and bone marrow transplantation for standardizing the study of chimerism in the therapy centers
Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea IMPORTANCE OF THE STUDY OF CHIMERISM IN THE ALLOGENEIC BONE MARROW TRANSPLANTATION
Ana M Amor Vigil,Gisela Martínez Antua
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: Desde que se comenzó a realizar el trasplante hematopoyético se reconoció la importancia de conocer el establecimiento de quimerismo. El presente trabajo actualiza los conceptos de quimerismo, su clasificación y las diferentes vías para su determinación. Se destaca como la más recomendada, la amplificación de zonas altamente polimórficas en el ADN por la técnica de la reacción en cadena de la polimerasa. La utilización de esta técnica ha permitido realizar estudios de la evolución de la quimera, relacionar el grado de quimerismo establecido con el comportamiento del injerto y de la enfermedad de injerto contra hospedero en los diferentes regímenes de acondicionamiento. También ha posibilitado la detección precoz de la recaída en los pacientes trasplantados y la administración oportuna de inmunoterapia adicional. Finalmente, se presentan las recomendaciones de la Sociedad Americana de Trasplante de Sangre y Médula ósea para estandarizar el estudio del quimerismo en los diferentes centros de tratamiento Since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. The present paper updates the concepts of chimerism, its classification and the different ways to determine it. The most recommended is the highly polymorphic area amplification in the DNA using the polymerase chain reaction technique. This technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. It has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. Finally, the paper presents the recommendations of the American Society of Blood and Bone Marrow Transplantation for standardizing the study of chimerism in the therapy centers
Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex
Amor Vigil,Ana M.; Matos Borges,Rafael; Martínez Antua,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: the clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the t-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. this type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. by the pcr-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the t-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. it was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. the usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex Diagnosis of cutaneous lymphomas by clonicity detection through PCR-heteroduplex technique
Ana M. Amor Vigil,Rafael Matos Borges,Gisela Martínez Antua
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: La detección de clonalidad en los síndromes linfoproliferativos mediante el estudio del reordenamiento de los genes de las inmuglobulinas y del receptor de células T, es utilizada para esclarecer si una proliferación o infiltrado de linfocitos es maligno o no. Este tipo de estudio es de particular utilidad en presencia de lesiones cutáneas cuyo origen linfoide o dermatológico resulta difícil de definir. Mediante la técnica de PCR-heterodúplex se estudiaron los genes de la cadena pesada de las inmunoglobulinas y de la cadena gamma del receptor de las células T, en 10 pacientes que presentaban manifestaciones dermatológicas atribuibles a algún tipo de linfoma cutáneo. Se observó reordenamiento clonal en 7 pacientes, lo cual permitió confirmar el diagnóstico de micosis fungoide y otros tipos de linfomas cutáneos. En 3 pacientes que no mostraron reordenamiento clonal, no fue posible confirmar por esta técnica un proceso linfoide de carácter maligno. Se demostró la utilidad del estudio cuando en presencia de una afección en la piel, es difícil diferenciar un proceso dermatológico de un síndrome linfoproliferativo con manifestaciones en piel. The clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the T-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. This type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. By the PCR-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the T-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. Clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. It was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. The usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Diagnóstico molecular de la leucemia aguda promielocítica: Resultados preliminares Molecular diagnosis of acute promyelocytic leukemia: preliminary results
Gisela Martínez Antua,Niubys Cayado Gutiérrez,Adriana Mu?iz Fernández,Edgardo Espinosa Martínez
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: La leucemia aguda promielocítica (LAP) se caracteriza por la presencia de la translocación recíproca t (15;17) que tiene como resultado la formación del gen híbrido PML-RARa . Como la LAP se considera una emergencia hematológica y además tiene hoy en día un tratamiento muy específico con ácido retinoico (ATRA), es muy importante hacer un diagnóstico rápido y preciso, que en muchos casos permite incluso salvar la vida del paciente. En la actualidad se han desarrollado métodos de RT-PCR para detectar el gen híbrido PML-RARa . Estas técnicas moleculares han sido extremadamente útiles en el diagnóstico de esta entidad. En este trabajo presentamos los resultados preliminares del diagnóstico molecular en 38 pacientes con LAP. En 36 pacientes se demostró la presencia del gen híbrido y 2 fueron negativos. Del total de enfermos con resultados positivos, 19 (55 %) fueron bcr 1, 2 (5 %) fueron bcr 2 y 14 (40 %) fueron bcr 3. Todos los pacientes con resultados positivos respondieron al tratamiento con ATRA. En 1 de los 2 pacientes con resultados negativos se demostró la presencia de la t(11;17). Ninguno de estos 2 enfermos respondió al tratamiento con ATRA Acute promyelocytic leukemia (APL) is characterized by the reciprocal translocation t(15; 17) that results in fusion gene PML-RARa formation. As APL is considered to be an hematological emergency and also is given a very specific treatment with retinoic acid (ATRA), then it is very important to diagnose quickly and accurately which makes it possible in many cases to save the patient's life. At present RT- PCR methods have been developed to detect PML-RARa gen. These molecular techniques have been extremely useful in diagnosing this entity. This paper sets forth the preliminary results of molecular diagnoses of APL in 38 patients. 36 cases presented the fusion gene and 2 did not. Of the total number of positive patients, 19(55%) were bcr 1, 2 (5%) were bcr 2 and 14(40%) bcr 3. All the patients with positive results were responsive to treatment with ATRA. One of the two patients with negative results showed the existence of t (11; 17). These two patients did not respond to treatment with ATRA
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