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Search Results: 1 - 10 of 1788 matches for " Eunize Maciel Zambolim "
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Heran?a da resistência do Híbrido de Timor UFV 443-03 à ferrugem-do-cafeeiro
Capucho, Alexandre Sandri;Caixeta, Eveline Teixeira;Zambolim, Eunize Maciel;Zambolim, Laércio;
Pesquisa Agropecuária Brasileira , 2009, DOI: 10.1590/S0100-204X2009000300009
Abstract: the aim of this work was to characterize the resistance inheritance of the timor hybrid ufv 443-03 to coffee leaf rust (hemileia vastatrix). for this, the race ii and pathotype 001 of coffee leaf rust were inoculated in 246 f2 plants, 115 susceptible backcrossing (bcs) plants, and 87 resistant backcrossing (bcr) plants, derived from the crossing between the susceptible genotype 'catuaí amarelo' iac 64 and the resistance source timor hybrid ufv 443-03. for both inoculums, the 'catuaí amarelo' iac 64 was susceptible, while the timor hybrid, the plant representing f1 generation, and the bcr plants were resistant. the f2 plants inoculated with race ii presented two significant segregation ratios: 15:1 and 61:3. the resistance inheritance was verified by the inoculation of the bcs plants, which segregated at a 3:1 ratio, an expected pattern for the inheritance controlled by two genes, whereas the segregation hypothesis of 7:1 for three genes was rejected. two independent and dominant genes confer the genetic resistance of timor hybrid ufv 443-03 to race ii and pathotype 001 of h. vastatrix.
NOTE-Polymorphic information content of SSR markers for Coffea spp.
Robson Fernando Missio,Eveline Teixera Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2010,
Abstract: Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzedin 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values werefound for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphicSSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and theleaf rust resistant arabica cultivars.
Development and validation of SSR markers for Coffea arabica L.
Robson Fernando Missio,Eveline Teixeira Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2009,
Abstract: With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries withprobes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences.Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were morefrequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1%of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSRmarkers were validated for further genetic studies of C. arabica.
Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies
Antonio Carlos Bai?o de Oliveira,Ney Sussumu Sakiyama,Eveline Teixeira Caixeta,Eunize Maciel Zambolim
Crop Breeding and Applied Biotechnology , 2007,
Abstract: A partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers.From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map. Seventeenmarkers were not linked, while 117 formed 11 linkage groups, covering a genome distance of 803.2 cM. The maximumdistance between adjacent markers was 26.9 cM, and only seven intervals exceeded 20 cM. The markers were further used forassisted selection of the plants closest to the recurrent parent, to accelerate the introgression of rust resistance genes in thecoffee breeding program. Three BC1 plants resistant to coffee leaf rust and with high genetic similarity to Catuaí wereselected and integrated in the following backcross cycles.
Surto epidemiológico do vírus do mosaico amarelo do piment o em tomateiro na regi o serrana do Espírito Santo
Maciel-Zambolim Eunize,Costa Helcio,S. Capucho Alexandre,ávila Ant?nio C. de
Fitopatologia Brasileira , 2004,
Abstract: Este trabalho relata a ocorrência de um surto epidemiológico causado pelo vírus do mosaico amarelo do piment o (Pepper yellow mosaic virus - PepYMV) em tomateiro (Lycopersicon esculentum) 'Alambra' na regi o serrana do Estado do Espírito Santo. Os sintomas consistiam de mosaico, definhamento e redu o de produ o. Visando a caracteriza o do agente causal foram realizados estudos sorológicos por ELISA, observa es ao microscópio eletr nico e determina o da gama parcial de hospedeiros. Ao microscópio eletr nico de transmiss o foram observadas, em amostras de tomateiro, partículas alongadas e flexuosas e inclus es cilíndricas típicas de vírus do gênero Potivirus. O PepYMV foi confirmado como agente causal por ELISA indireto. Levantamentos realizados em campos de cultivo demonstraram que a dissemina o do vírus é muito rápida. Este é o primeiro relato da ocorrência do PepYMV na cultura do tomate no Brasil, causando sérios danos.
Detection and partial characterization of an isolate of Groundnut ringspot virus in Solanum sessiliflorum
Fitopatologia Brasileira , 2002,
Abstract: The cubiu (Solanum sessiliflorum) fruit, originating in the Amazon basin, is commonly used in that region for food, medicine, and cosmetics. In an experimental culture of cubiu, in order to evaluate its adaptation to conditions in the Northern region of the state of Rio de Janeiro, it was observed plants with mosaic symptoms. A cubiu plant was collected and analyzed to identify the etiological agent. After mechanical passage through a local lesion host, a host range test was performed. The virus induced chlorotic local lesions in Chenopodium quinoa, necrotic local lesions in Gomphrena globosa, mosaic in S. sessiliflorum, leaf and stem necrosis in tomato (Lycopersicon esculentum) 'Rutgers', mosaic and leaf distortion in Datura stramonium and Physalis floridana, and necrotic local lesions followed by systemic necrosis and plant death in four Nicotiana species. Electron microscopic observations of ultra thin sections from infected cubiu leaves showed the presence of spheroidal, membrane-bound particles typical of tospovirus species. Analysis of the nucleocapsid protein from concentrated virus particles indicated the presence of a 28 kDa protein. RT-PCR was performed after total RNA extraction from infected IPA-6 tomato leaves. A fragment of approximately 0,8 kbp corresponding to the N gene was amplified, cloned and sequenced. The N protein from the cubiu isolate was 95% homologous to the Groundnut ringspot virus (GRSV) protein, and no more than 85% homologous to those from Zucchini lethal chlorosis virus (ZLCV) and Chrysanthemun stem necrosis virus (CSNV), Tomato spotted wilt virus (TSWV), and Tomato chlorotic spot virus (TCSV). This is the first report of the occurrence of GRSV (or any other plant virus) in cubiu.
Breeding potential and genetic diversity of "Híbrido do Timor" coffee evaluated by molecular markers
Setotaw, Tesfahun Alemu;Caixeta, Eveline Teixeira;Pena, Guilherme Ferreira;Zambolim, Eunize Maciel;Pereira, Antonio Alves;Sakiyama, Ney Sussumu;
Crop Breeding and Applied Biotechnology , 2010, DOI: 10.1590/S1984-70332010000400003
Abstract: aflp, rapd and ssr molecular markers were used to study the genetic diversity and genetic structure of the híbrido de timor germplasm. the principal coordinate analysis, upgma cluster analysis based on genetic dissimilarity of jaccard, bayesian model-based cluster analysis, percentage of polymorphic loci, shannon's information index and nei gene diversity were employed to assess the genetic diversity. the analyses demonstrated a high genetic diversity among híbrido de timor accessions. upgma and bayesian cluster analyses grouped the accessions into three clusters. the genetic structure of híbrido de timor is reported. the management of híbrido de timor germplasm variability and its potential use in breeding programs are discussed.
Assessment of EST-SSR markers for genetic analisys on coffee
Missio, Robson Fernando;Caixeta, Eveline Teixeira;Zambolim, Eunize Maciel;Pena, Guilherme Ferreira;Ribeiro, Ana Paula;Zambolim, Laércio;Pereira, Ant?nio Alves;Sakiyama, Ney Sussumu;
Bragantia , 2009, DOI: 10.1590/S0006-87052009000300003
Abstract: est-ssr markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. seventeen markers, developed from ests (expressed sequence tags) from the brazilian coffee genome project, were used. all markers showed polymorphism among the genotypes assessed. the average number of allele per primer was 5.1. the highest polymorphisms were found within c. canephora (88.2%) and rust-resistant varieties (35.3%). about 29.4% of the markers differentiated c. arabica from híbrido de timor; it was also possible to identify those closest and farthest from c. arabica . the analysis of population-grouped genotypes revealed a 64.0% genetic diversity among and a 36.0% genetic diversity within populations. the differentiation index was 0.637. six markers distinguished four rust-resistance varieties, showing their fingerprinting potential. these results demonstrate the usefulness of est-ssr markers for cross orientation, in diversity and introgression studies, and in genetic mapping.
Marcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem
Alvarenga, Samuel Mazzinghy;Caixeta, Eveline Teixeira;Hufnagel, Bárbara;Thiebaut, Flávia;Maciel-Zambolim, Eunize;Zambolim, Laércio;Sakiyama, Ney Sussumu;
Pesquisa Agropecuária Brasileira , 2011, DOI: 10.1590/S0100-204X2011000800015
Abstract: the objective of this work was to identify molecular markers related to the resistance of coffee (coffea arabica) to rust (hemileia vastatrix). dna sequences potentially involved in coffee disease resistance were identified, using "in silico" analysis, from data obtained by the brazilian coffee genome project. after data mining, 59 primer pairs were designed to amplify the sequences identified. the 59 primers were tested on 12 resistant and 12 susceptible coffee plants to h. vastatrix. twenty-seven primers resulted in unique and well-defined bands, while one of these amplified a dna fragment in all resistant plants, but not in the susceptible ones. this polymorphic molecular marker amplified a region of dna that corresponds to a partial open reading frame of c. arabica genome that encodes a disease resistance protein. the marker carf 005 can be used to differentiate between resistant and susceptible coffee plants to h. vastatrix.
Surto epidemiológico do vírus do mosaico amarelo do piment?o em tomateiro na regi?o serrana do Espírito Santo
Maciel-Zambolim, Eunize;Costa, Helcio;S. Capucho, Alexandre;ávila, Ant?nio C. de;Inoue-Nagata, Alice K.;Kitajima, Elliot W.;
Fitopatologia Brasileira , 2004, DOI: 10.1590/S0100-41582004000300017
Abstract: an outbreak of pepper yellow mosaic virus (pepymv) was observed in commercial fields of tomato (lycopersicon esculentum) 'alambra' in the serrana region of espírito santo, brazil. symptoms consisted of intense yellow mosaic, weakening of the infected plants and reduction of the yield. the virus was identified by host range, electron microscopy and indirect elisa. field surveys indicated a rapid spread of the virus. this is the first report of natural infection of tomato by pepymv causing severe damage to this crop in brazil.
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