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Search Results: 1 - 10 of 11587 matches for " Eun Ha Kang "
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NS-398 induces caspase-dependent, mitochondria-mediated intrinsic apoptosis of hepatoma cells  [PDF]
Il Han Song, Suk Bae Kim, Hyun Duk Shin, Ha Yan Kang, Eun Young Kim
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.326084
Abstract: The present study was conducted to investigate whether mitochondrial pathway of apoptosis is involved in cyclooxygenase-2 (COX-2) inhibitor-induced growth inhibition of hepatoma cells. The growth rate and pattern of NS-398 (selective COX-2 inhibitor)-treated Hep3B hepatoma cells were analyzed by microscopic examination, DNA fragmentation gel analysis and flow cytometry followed by the cleavage of down-stream caspase 3 and the release of cytosolic fraction of cytochrome c assessed by Western blot analysis. NS-398 induced the growth inhibition of hepatoma cells depending on the concentration of this COX-2 inhibitor and time sequence. Ladder patterned-DNA fragmentation and cytometric redistribution to sub-G1 phase in cell cycle were revealed in NS-398-induced growth inhibition of hepatoma cells. Cytochrome c was translocated from mitochondria to cytosol in time-dependent manner following NS-398 treatment to hepatoma cells. COX-2 inhibitor induces the growth inhibition of hepatoma cells via caspase-dependent, mitochondria-mediated intrinsic apoptosis pathway. These results strongly suggest the possibility of therapeutic implication of COX-2 inhibitor in HCC.
Downregulation of heat shock protein 70 protects rheumatoid arthritis fibroblast-like synoviocytes from nitric oxide-induced apoptosis
Eun Ha Kang, Dong Jo Kim, Eun Young Lee, Yun Jong Lee, Eun Bong Lee, Yeong Wook Song
Arthritis Research & Therapy , 2009, DOI: 10.1186/ar2797
Abstract: Targeted knock-down of Hsp70 was performed by RNA interference in RA FLSs at passage 3-7. After SNP treatment, the morphological features of apoptosis were observed by phase-contrast microscopy. Cell survival was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays and by flow cytometric analysis after propidium iodide (PI) staining. Bcl-2 expression and signaling pathways (Akt, extracellular signal-regulated kinase, p38, c-Jun N-terminal kinase) were examined with or without Hsp70 downregulation.Hsp70 downregulation in RA FLSs, induced by small interfering RNA (siRNA), was confirmed by reverse transcriptase-polymerase chain reaction and Western blotting. When treated with SNP, Hsp70 downregulated cells showed markedly less cell blebbing, cytoplasmic condensation, and nuclear shrinkage than non-downregulated control cells. Furthermore, Hsp70 downregulated cells were found to survive better than control cells in MTT assays (mean of absorbance ratio, 4.39 in target cells versus 1.00 in control siRNA-treated cells versus 1.09 in lipofectamine-treated cells, P = 0.001) and according to PI staining results (mean M1 ratio, 0.21 in target cells versus 1.00 in control siRNA-treated cells versus 1.03 in lipofectamine-treated cells, P = 0.001). Bcl-2 expression and Akt phosphorylation were higher in Hsp70 downregulated RA FLSs than in control cells. When cells were treated with LY294002, a potent phosphoinositide 3-kinase inhibitor, Akt phosphorylation and Bcl-2 levels were reduced and Hsp70 downregulation no longer had a cytoprotective effect.Knock-down of Hsp70 protects RA FLSs from nitric oxide-induced apoptosis by activating the Akt signaling pathway. These results suggest that Hsp70 has a pro-apoptotic role in RA FLSs.Rheumatoid arthritis (RA) is a chronic inflammatory disorder that involves mainly joint synovium. One of the major characteristics of RA synovium is the tumor-like growth of fibroblast-like synoviocytes (FLSs) that invade ad
Raman Scattered HeII 6545 in the Young and Compact Planetary Nebula NGC 6790
Eun-Ha Kang,Hee-Won Lee,Byeong-Cheol Lee
Physics , 2009, DOI: 10.1088/0004-637X/695/1/542
Abstract: We present the high resolution spectra of the youn and compact planerary nebula NGC 6790 obtained with the echelle spectrograph at Bohyunsan Optical Astronomy Observatory and report the discovery of Raman scattered He II 6545 in this object. This line feature is formed in a thick neutral region surrounding the hot central star, where He II 1025 line photons are scattered inelastically by hydrogen atoms. A Monte Carlo technique is adopted to compute the line profile with a simple geometric model, in which the neutral region is in the form of a cylindrical shell that is expanding from the central star. From our line profile analysis, the expansion velocity of the HI region lies in the range V_exp = 15- 19 Km/s. Less stringent constraints are put on the HI column density N_HI and covering factor C, where the total flux of Raman He II 6545 is consistent with the product of N_HI and C being CN_HI ~ 0.5 X 10^20 cm^-2. The Monte Carlo profiles from stationary emission models exhibit deficit in the wing parts. A much better fit is obtained when the He II emission region is assumed to take the form of a ring that slowly rotates with a rotation speed ~ 18Km/s. Brief discussions are presented regarding the mass loss processes and future observations.
Associations between the HLA-A polymorphism and the clinical manifestations of Behcet's disease
Eun Ha Kang, Jeong Yeon Kim, Fujio Takeuchi, Joon Wan Kim, Kichul Shin, Eun Young Lee, Yun Jong Lee, Eun Bong Lee, Myoung Hee Park, Yeong Wook Song
Arthritis Research & Therapy , 2011, DOI: 10.1186/ar3292
Abstract: Genotyping for the HLA-A locus was performed using the polymerase chain reaction-Luminex typing method in 223 BD patients and 1,398 healthy controls.The phenotypic frequencies of HLA-A*02:07 (odds ratio (OR) = 2.03, P = 0.002), A*26:01 (OR = 1.85, P = 0.008), and A*30:04 (OR = 2.51, P = 0.006) tended to be higher in BD patients than in normal controls, but the frequency of A*33:03 (OR = 0.59, P = 0.003) tended to be lower in BD patients. A meta-analysis adopting our and the Japanese data confirmed the associations of HLA-A*02:07, A*26:01, and A*33:03 with BD. Furthermore, the frequencies of the HLA-A*02:07, A*26:01, and A*30:04 were significantly higher in patients with skin lesions (OR = 2.37, P < 0.0005, Pc < 0.012) and arthritis (OR = 2.32, P = 0.002, Pc = 0.048), with uveitis (OR = 3.01, P < 0.0005, Pc < 0.012), and with vascular lesions (OR = 9.80, P < 0.0005, Pc < 0.012) and a positive pathergy test (OR = 4.10, P = 0.002, Pc = 0.048), respectively, than in controls. In HLA-B*51 non-carriers, these associations were also significant, being much stronger between HLA-A*26:01 and uveitis (OR = 4.19, P < 0.0005, Pc < 0.012) and between HLA-A*30:04 and vascular lesions (OR = 13.97, P < 0.00005, Pc < 0.0012). In addition, HLA-A*30:04 was associated with genital ulcers in HLA-B*51 non-carriers (OR = 3.89, P = 0.002, Pc = 0.048).HLA-A*02:07, A*26:01, and A*30:04 were associated with increased risk for BD, while HLA-A*33:03 with decreased risk. HLA-A*02:07, A*26:01, and A*30:04 were associated with skin lesions and arthritis, with uveitis, and with vascular lesions, genital ulcers, and a positive pathergy test, respectively.Behcet's disease (BD) is a chronic relapsing inflammatory disease characterized by oro-genital ulcers, cutaneous inflammation, and uveitis. In addition to its typical muco-cutaneous and ocular manifestations, BD targets the musculoskeletal, vascular, nervous, and gastrointestinal systems [1]. Although the etiology of BD remains unclear, strong famili
Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceus
Eun-Mi Park, Jung-Ha Kang, Jung Seo, GunDo Kim, Jongkyeong Chung, Tae-Jin Choi
BMC Immunology , 2008, DOI: 10.1186/1471-2172-9-31
Abstract: A DNA fragment containing the conserved SH2 domain was amplified by RT-PCR using degenerate primers designed based on the highly conserved sequences in the SH2 domains of the zebrafish and mammalian STAT1. The complete cDNA sequence was obtained by 5' and 3' RACE. The flounder STAT1 transcript consisted of 2,909 bp that encoded a polypeptide of 749 amino acids. The overall similarity between flounder STAT1 and other STATs was very high, with the highest amino acid sequence identity to snakehead (89%). Phylogenetic analyses reveal that flounder STAT1 is in the same monophyletic group with snakehead STAT1. Quantitative real time RT-PCR and in situ hybridization revealed that STAT1 was expressed in almost all examined organs and tissues, with high expression in gill, spleen, kidney, and heart. The accumulation of STAT1 mRNA in different developmental stages, as determined by real time RT-PCR, increased with development.Recent cloning of various cytokine genes and the STAT1 gene of olive flounder here suggest that fish also use the highly specialized JAK-STAT pathway for cytokine signaling. Identification of other STAT genes will elucidate in detail the signal transduction system in this fish.Cellular responses to internal and external signals are mediated by the expression of specific genes or sets of genes, which are regulated by specific transcriptional factors [1]. Therefore, the entry of transcription factors into the nucleus is critical to their role in gene expression. Signals from cytokines and growth factors are transduced into the nucleus by the Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling pathway [2-6]. JAK-STAT signaling is also involved in the regulation of cell proliferation, differentiation, survival, motility, and apoptosis in different organs [7].The binding of signal molecules to their receptors initiates activation of JAKs, which increases their tyrosine kinase activity [4,8]. The activated JAKs phosphorylate
Efficacy of Lactic Acid Bacteria (LAB) supplement in management of constipation among nursing home residents
Hyang An, Eun Baek, Seok Jang, Do Lee, Mi Kim, Jung Kim, Kang Lee, Jong Park, Nam Ha
Nutrition Journal , 2010, DOI: 10.1186/1475-2891-9-5
Abstract: Nineteen subjects (8M, 11F; mean age 77.1 ± 10.1) suffering with chronic constipation were assigned to receive LAB (3.0 × 1011 CFU/g) twice (to be taken 30 minutes after breakfast and dinner) a day for 2 weeks in November 2008. Subjects draw up a questionnaire on defecation habits (frequency of defecation, amount and state of stool), and we collected fecal samples from the subjects both before entering and after ending the trial, to investigate LAB levels and inhibition of harmful enzyme activities. Results were tested with SAS and Student's t-test.Analysis of questionnaire showed that there was an increase in the frequency of defecation and amount of stool excreted in defecation habit after LAB treatment, but there were no significant changes. And it also affects the intestinal environment, through significantly increase (p < 0.05) fecal LAB levels. In addition, tryptophanase and urease among harmful enzyme activities of intestinal microflora were significantly decreased (p < 0.05) after LAB treatment.LAB, when added to the standard treatment regimen for nursing home residents with chronic constipation, increased defecation habit such as frequency of defecation, amount and state of stool. So, it may be used as functional probiotics to improve human health by helping to prevent constipation.Constipation is prevalent in modern societies and is a common symptom in clinical practice [1].Constipation involves the large intestine and is a symptom rather than a disease. It is characterized by a constellation of symptoms and complaints, the most common of which are low defecation frequency (e.g. less than 3/week), irregular stool expulsion, painful and strained defecation, hard and dry stool consistency, a feeling of incomplete rectal defecation, and passing of abnormally small stools (e.g. less than 50 g/day) [2].The prevalence of constipation and its impact on quality of life are most significant among elderly individuals, with a reported incidence among ambulatory adult
Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content
Do Lee, Seok Jang, Eun Baek, Mi Kim, Kyung Lee, Hea Shin, Myung Chung, Jin Kim, Kang Lee, Nam Ha
Lipids in Health and Disease , 2009, DOI: 10.1186/1476-511x-8-21
Abstract: In vitro culture experiments were performed to evaluate the ability of Bifidobacterium spp. isolated from healthy Koreans (20~30 years old) to reduce cholesterol-levels in MRS broth containing polyoxyethanylcholesterol sebacate. Animal experiments were performed to investigate the effects on lowering cholesterol, inhibiting harmful enzyme activities, and controlling fecal water content. For animal studies, 0.2 ml of the selected strain cultures (108~109 CFU/ml) were orally administered to SD rats (fed a high-cholesterol diet) every day for 2 weeks.B. longum SPM1207 reduced serum total cholesterol and LDL levels significantly (p < 0.05), and slightly increased serum HDL. B. longum SPM1207 also increased fecal LAB levels and fecal water content, and reduced body weight and harmful intestinal enzyme activities.Daily consumption of B. longum SPM1207 can help in managing mild to moderate hypercholesterolemia, with potential to improve human health by helping to prevent colon cancer and constipation.Probiotic bacteria have multiple potential health effects, including blocking gastroenteric pathogens [1-4], neutralizing food mutagens produced in the colon [1,5-10], enhancing the immune response [6,9,11-14], lowering serum cholesterol, and stopping intestinal dysfunction [15-21]. In general, probiotic bacteria must colonize the gastrointestinal tract (GIT) of the host, have acid- and bile salt-tolerance, and block putrefactive bacteria in the GIT. Lactic acid bacteria (LAB), especially Lactobacillus spp. and Bifidobacterium spp. are important GIT residents and are used as probiotic strains to improve health [22-24]. Lactobacillus and Bifidobacterium have been used in fermented foods for several centuries without adverse effects [25,26] and are classified as Generally Recognized as Safe (GRAS) because of their long history of safe use, particularly in dairy foods [27,28].Here, we evaluated the ability of Bifidobacteria spp. isolated from healthy Koreans (20~30 years old) to
Zoeal Stages of Leptomithrax edwardsii (Crustacea: Decapoda: Majidae) Described from Laboratory Reared Material
Jung-Ha Kang,Yong-Seok Lee,Ji-Eun Jeong,Hyun-Sook Ko3
Animal Systematics, Evolution and Diversity , 2012, DOI: http://dx.doi.org/10.5635/ased.2012.28.3.185
Abstract: Zoeas of Leptomithrax edwardsii were reared in the laboratory. Two zoeal stages are described and illustrated. The first zoeal stage of L. edwardsii is compared with those of seven known species of the family Majidae. It differs from previous description in the endopodal setation characters of the maxillule and the second maxilliped. It appears most similar to L. bifidus and Schizophroida simodaensis of the northwestern Pacific. A provisional key for identifying eight majid zoeas is included.
A Novel Antibacterial Compound from Siegesbeckia glabrescens
Young-Soo Kim,Hyungil Kim,Eunsun Jung,Jang-Hyun Kim,Wangtaek Hwang,Eun-Ju Kang,Sanghyun Lee,Byung-Jo Ha,Jongsung Lee,Deokhoon Park
Molecules , 2012, DOI: 10.3390/molecules171112469
Abstract: The crude methanol extract of the dried aerial parts of Siegesbeckia glabrescens (Compositae) showed antibacterial activity against the foodborne pathogen Staphylococcus aureus. Bioactivity-guided separation led to the isolation of 3-(dodecanoyloxy)-2-(isobutyryloxy)-4-methylpentanoic acid from nature for the first time. The structure was determined by spectroscopic data analysis (UV, MS, and NMR). The minimal inhibitory concentration (MIC) of 3-(dodecanoyloxy)-2-(isobutyryloxy)-4-methylpentanoic acid against S. aureus was found to be 3.12 μg/mL. In addition, in a further antimicrobial activity assay against Gram-positive (B. subtilis, E. faecalis, P. acnes, S. epidermidis, S. schleiferi subsp. coagulans, S. agalactiae and S. pyrogens), and Gram-negative bacteria (E. coli and P. aeruginosa), and yeast strains (C. alibicans and F. neoformans), the antimicrobial activity of the compound was found to be specific for Gram-positive bacteria. The MIC values of the compound for Gram-positive bacteria ranged from 3.12 to 25 mg/mL. Furthermore, it was found that the 2-(isobutyryloxy)-4-methylpentanoic acid substituent may operate as a key factor in the antibacterial activity of the compound, together with the laurate group.
Interventional microadhesiolysis: A new nonsurgical release technique for adhesive capsulitis of the shoulder
Kang Ahn, Young-Jin Lee, Eun-Ha Kim, Seung-Min Yang, Tae-Kyun Lim, Yong-Soo Kim, Hyung-Joon Jhun
BMC Musculoskeletal Disorders , 2008, DOI: 10.1186/1471-2474-9-12
Abstract: Ten patients (five men and five women) with primary adhesive capsulitis of the shoulder were treated at a chronic pain management center in Korea. Three specially made needles are used in interventional microadhesiolysis: the Round, Flexed Round, and Ahn's needles. A Round Needle is inserted on the skin over middle of supraspinatus and advanced under the acromion and acromioclavicular joint (subacromial release). A Flexed Round Needle is inserted two-fingers caudal to the inferior border of the scapular spine and advanced over the capsule sliding on the surface of infraspinatus muscle-tendon fascia. The capsule is released while an assistant simultaneously passively abducts the shoulder to full abduction (posteroinferior capsule release). An Ahn's Needle is inserted on the skin over the lesser tubercle and advanced under the coracoid process sliding on the surface of the subscapularis muscle (subcoracoid release).After the patients underwent interventional microadhesiolysis, the self-rated pain score or severity declined significantly (p < .01), the shoulder range of motion increased significantly (p < .01), and joint effusion in the affected shoulder decreased or disappeared in nine of ten patients on magnetic resonance imaging compared to their initial status.Our findings suggest that interventional microadhesiolysis is effective for managing adhesive capsulitis of the shoulder.Adhesive capsulitis of the shoulder is characterized by pain in the shoulder and limitation of glenohumeral movement. It tends to occur in patients older than 40 years of age and most commonly in females in their 50s. It has been reported that adhesive capsulitis lasts 2–3 years [1]. Even after this period, however, resolution may not be complete, and many studies have shown mild to moderate restriction of motion [2].Various interventions have been developed to treat this annoying condition, including oral medication, corticosteroid injection, physical therapy, nerve blocks, manipulation un
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