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Gastric Cancer Prevalence, According To Survival Data in Iran (National Study-2007)
AA Mehrabian,F Esna-Ashari,H Zham,M Hadizadeh
Iranian Journal of Public Health , 2010,
Abstract: "nBackground: Gastric cancer is a common and lethal disease throughout the world. In Iran with 7300 new cases annually, it is the first cause of cancer related death in both sexes. Regarding the high incidence (10.5/100000 individuals) of gastric can-cer and priority of prevalence index in cancer management, in this study we tried to determine 1, 2-3 and 4-5 year point preva-lence of the disease according to survival data."nMethod: Survival and incidence data were used for determination of cancer prevalence. Incidence data were extracted from can-cer registry in Iran and survival data were determined in a descriptive study by following up 3439 gastric cancer patients in Cancer Research Center (CRC). 1, 2-3 and 4-5 year prevalence was estimated from incidence rates in different years and the proportion of patients surviving 0.5, 1.5, 2.5, 3.5 and 4.5 years from the time of diagnosis."nResults: patients with survival of 0.5, 1.5, 2.5, 3.5 and 4.5 years from the time of diagnosis were 46.38%, 26 %, 19.36%, 15.47% and 12.8% respectively. The prevalence of 1, 2-3 and 4-5 year was 3392, 3118and 1824 respectively. The cumula-tive 5 year prevalence was 8334 cases."nConclusion: These estimates of the point prevalence of gastric cancer at 1, 2-3 and 4-5 years are applicable to the evalua-tion of initial treatment, clinical follow-up, and point of cure. Therefore 1, 2-3 and 4-5 year point prevalence estimates, are neces-sary in health service planning for gastric cancer management and should be considered by public health managers.
Semi-Quantitative Analysis of Endometrial HOXA10 and BTEB1 Mrna Expressions in the Implantation Window of Patients With Endometriosis and Myoma
Shokrzadeh Sh,Saidijam M,Dehghan A,Esna-Ashari F
Tehran University Medical Journal , 2012,
Abstract: Background: The techniques used in assisted reproductive technologies have progressed considerably, but many embryos do not implant after transfer upon the use of these techniques. One of the causes of infertility is repeated implantation failure due to decreased endometrial receptivity. Furthermore, in clinical conditions such as endometriosis and myoma, implantation decreases after embryo transfer. In this case-control study the expression patterns of HOXA-10 and BTEB1 mRNAs were evaluated at the time of implantation in patients with myoma and endometriosis.Methods : In this study performed in Hamadan University of Medical Sciences during 1389, the cases included 16 patients with endometriosis and myoma (8 in each group) and the control group consisted of 8 fertile women. Endometrial sampling was performed at mid-secretory phase. Later, the expression patterns of HOXA-10 and BTEB1 mRNAs were evaluated using a semi-quantitative RT-PCR method. Results : The optimal PCR cycles determined were 30, 32 and 26 for HOXA10, BTEB1 and β-actin, respectively. Endometrial HOXA-10 and BTEB1 mRNA expression levels (normalized to -actin expression) at the time of implantation were significantly decreased in the endometrium of infertile patients with endometriosis compared with that of healthy fertile controls (P<0.05). A similar pattern was seen in patients with myomas for both HOXA10 and BTEB1 genes, (P<0.05). Conclusion: It seems that lower expression of HOXA-10 and BTEB1 mRNAs in the implantation window of endometrium that increase normally, could account for some aspects of infertility in patients with endometriosis and myoma.
A Retrospective Evaluation of Epidemiological, Clinical and Laboratory Features of Brucellosis in 230 Patients in Hamadan, Iran: A Brief Report
P Eini,F Esna-Ashari,AR Mobaien,M Hasanzadeh
Tehran University Medical Journal , 2012,
Abstract: Background: Brucellosis is one of the most common infectious diseases in Iran with very different clinical manifestations. Methods: In this retrospective descriptive study, all patients with brucellosis, who were admitted in Farshchian Hospital in Hamadan, Iran in 2005 to 2010, were enrolled in the study. The data were collected from the patients' medical records and were entered in forms for analysis. Results: A total of 230 patients with brucellosis, including 130 (56.5%) male and 100 (43.5%) female patients with a mean age of 40.84±20.29 years, who mostly (72.2%) lived in rural areas were enrolled in the study. Outbreaks were most common in spring and summer and the main route of transmission was consumption of contaminated dairy products (60.3%). The most common symptoms were fever (77.4%), arthralgia (70%), sweating (47%), malaise and fatigue (46.5%). Arthritis and epididymo- orchitis were seen in 121 (52.9%) and 48 (8.20%) patients, respectively. CBC analysis showed leukocytosis in 20.8% of the participants. ESR rise was noted in 59.5% of the patients and 52.9% had positive CRP. Conclusion: Given to various clinical presentations, brucellosis should be considered in the differential diagnosis of individuals with chronic fever with or without other organ abnormalities.
Microtitration of Rubella Virus in Monovalent Vaccinal Products
F Esna-Ashari,A Shafyi,M Taqavian,A Mohammadi
Iranian Journal of Public Health , 2011,
Abstract: "nBackground: Potency test for control of rubella vaccine is a significant factor to qualify production line and vaccination pro-gram. For this reason, WHO recommends to use the microtitration method by both vaccine companies and control laborato-ries. Then the study was done to improve this test."nMethods: Three rubella virus samples, including an in-house standard, a lot of vaccine and an in- process product, were tit-tered in cell culture tubes. Then micro titration steps were tested on 96-well microplate using cocultivation of standard ru-bella vaccine dilutions and RK- 13 cell line. After 6-7 days, final reading was done and calculated the titer. Two other sam-ples were assayed with the micromethod."nResults: Titer reduction less than 0.5 log was acquired for each sample during frequent tests and between two methods."nConclusion: The procedure was profitable and accurate for potency and identity tests of rubella virus vaccine, on the basis of WHO recommendations.
Modeling Isosteric Heat of Soya Bean for Desorption Energy Estimation Using Neural Network Approach
Amiri Chayjan,Reza; Esna-Ashari,Mahmood;
Chilean journal of agricultural research , 2010, DOI: 10.4067/S0718-58392010000400012
Abstract: sorption isotherm of soya bean (glycine max (l.) merr.) was obtained by the dynamic experimental method. artificial neural networks (anns) were used for modeling soya bean equilibrium moisture content (emc). thermodynamic equations and trained ann for prediction of two thermodynamic properties of net isosteric heat and entropy of soya bean were utilized. the ann models were better compared with mathematical models. in this study, the isosteric heat and entropy of sorption of soya bean were separately predicted by two power models as a emc function. predictive power of the models was high (r2 ≈ 0.99). at the moisture content above 11% (dry basis, db), isosteric heat and entropy of sorption of soya bean were smoothly decreased, while they were highest at moisture content about 8% (db). isosteric heat and entropy would be useful in the storage simulation of dried soya bean. the ann model predicts soya bean emc more accurately than mathematical models. hence, better equations could be developed for the prediction of heat of sorption and entropy based on data from the ann model.
Modeling Isosteric Heat of Soya Bean for Desorption Energy Estimation Using Neural Network Approach Modelación de Calor Isostérico de Soya para Estimación de Energía de Desorción usando Red neural
Reza Amiri Chayjan,Mahmood Esna-Ashari
Chilean Journal of Agricultural Research , 2010,
Abstract: Sorption isotherm of soya bean (Glycine max (L.) Merr.) was obtained by the dynamic experimental method. Artificial Neural Networks (ANNs) were used for modeling soya bean equilibrium moisture content (EMC). Thermodynamic equations and trained ANN for prediction of two thermodynamic properties of net isosteric heat and entropy of soya bean were utilized. The ANN models were better compared with mathematical models. In this study, the isosteric heat and entropy of sorption of soya bean were separately predicted by two power models as a EMC function. Predictive power of the models was high (R2 ≈ 0.99). At the moisture content above 11% (dry basis, db), isosteric heat and entropy of sorption of soya bean were smoothly decreased, while they were highest at moisture content about 8% (db). Isosteric heat and entropy would be useful in the storage simulation of dried soya bean. The ANN model predicts soya bean EMC more accurately than mathematical models. Hence, better equations could be developed for the prediction of heat of sorption and entropy based on data from the ANN model. La isoterma de desorción de soya (Glycine max (L.) Merr.) se obtuvo por el método experimental dinámico. Se usaron redes neuronales artificiales (ANN) para modelación del contenido de humedad de equilibrio (EMC) de soya. Se utilizaron ecuaciones termodinámicas y ANN dirigidas para predicción de dos propiedades termodinámicas, calor isostérico neto y entropía de soya. Los modelos ANN fueron mejores comparados con modelos matemáticos. En este estudio, el calor isostérico y entropía de absorción de soya fueron predichas separadamente por dos modelos de poder como una función de EMC. El poder predictivo de los modelos fue alto (R2 ≈ 0.99). A un contenido de humedad superior a 11% (db), el calor isostérico y entropía de absorción de soya fueron ligeramente reducidos, y fueron las más altas a un contenido de 8% (db). El calor isostérico y entropía podrían ser útiles en la simulación del almacenamiento de soya deshidratada. El modelo ANN predice EMC de soya más confiablemente que los modelos matemáticos. Por lo tanto, podrían desarrollarse mejores ecuaciones para la predicción del calor de absorción y entropía basada en datos del modelo ANN.
Determining Chemical Composition and Antimicrobial Activity of Feverfew (Tanacetum parthenium L.) Essential Oil on Some Microbial Strains
Zahra Izadi,Majid Aghaalikhani,Mahmood Esna-Ashari,Poorandokht Davoodi
Zahedan Journal of Research in Medical Sciences , 2013,
Abstract: Background: Feverfew (Tanacetum parthenium L.) is a herbal plant that has anti- septic, anti-microbial, anti-parasitic and anti-inflammatory effects. The main objective of this study is to evaluate the antimicrobial effect of shoot essential oil (essential oil of the aerial parts of the plant) of the feverfew on a number of microorganisms including gram-negative and gram-positive bacteria, filamentous fungi and yeasts. Materials and Methods: In this empirical study, plant samples were collected at the full blooming stage. Shoot essential oil was extracted using hydro-distillation technique and Clevenger apparatus. Components of the extract were identified using GC and GC/MS apparatus and its antimicrobial properties were evaluated using diffusion in Agar method (disk diffusion) and dilution in the well (Micro-broth dilution).Results: Among 35 compounds identified in the essential oil of the feverfew, camphor (45%), chrysanthenyl acetate (21.5) and camphene (9.6%), were the main components respectively. Essential oil showed very good antifungal effect which was stronger than its antibacterial effect. Gram-negative bacteria were less sensitive to the essential oil than gram-positive bacteria. The mean diameter of inhibition zone, in the bio-assessment of the effect of feverfew essential oil on gram-positive bacteria and fungi was respectively more than the effect of vancomycin and amphotericin B and this effect on gram-negative bacteria was less than the effect of gentamicin. This effect is attributed to the high value of camphor, chrysanthenyl acetate and camphene found in the essential oil.Conclusion: Feverfew essential oil could be utilized as a sound and harmless substitute for the antibiotics.
Effect of Mannitol on Growth of Embryogenic Callus-Derived Somatic Embryos of Carnation (Dianthus Caryophyllus L.)
A. Deljou,O. Karami,M. Esna-Ashari
Journal of Science and Technology of Agriculture and Natural Resources , 2007,
Abstract: In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30 g/l-1 sucrose alone or supplemented with different concentrations of mannitol (15, 30, 60, 90, 120 and 150 gl-1). No somatic embryo was formed on culture media containing mannitol without sucrose. Number of somatic embryos produced from embryogenic calli significantly increased by adding mannitol to the culture media. Normal embryos formed on culture media containing high concentrations of mannitol (60, 90, 120 and 150 gl-1) developed normally. About 95% of somatic embryos transferred to the1/2 MS culture medium containing 30 gl-1 sucrose, germinated into plantlets. Plantlets also continued their growth under greenhouse conditions.
Effect of Putrescine Application on Post-Harvest Life and Physiology of Strawberry, Apricot, Peach and Sweet Cherry Fruits
M. R. Zokaee Khosroshahi,M. Esna-Ashari
Journal of Science and Technology of Agriculture and Natural Resources , 2008,
Abstract: Effect of different exogenous putrescine concentrations on post-harvest life of strawberry, apricot, peach and sweet cherry fruit was separately studied using completely randomized designs in three replicates. Fruits were immerged in putrescine solutions (0.3, 0.5, 1 and 2 mM for 5 minutes in strawberries, and 0.5, 1, 2, 3 and 4 mM for 10 minutes in the other fruits) and also in distilled water as control. They were then transferred into the fridge. Post-harvest life of all fruits was increased by the use of putrescine. Ethylene production and water loss of the fruits were reduced by this compound. Putrescine also prevented softening of fruit's tissue during their storage period. Titratable acidity was reduced in untreated fruits while their pH was increased. These trends were also observed with the application of putrescine in all fruits, but at a much slower rate compared with the controls. Soluble solids content of sweet cherry fruits was increased by the use of putrescine while it was reduced in the other three fruits.
Effect of Methyl Jasmonate on Resveratrol Production in Leaf and Fruit of Two Iranian Grape ( Vitis vinifera L.) Cultivars
A. Mahmoodi Pour,M. Esna-Ashari,O. Karami,M. Hesari
Journal of Science and Technology of Agriculture and Natural Resources , 2008,
Abstract: Resveratrol (5,3,4'- trihyroxystilbene) is a valuable polyphenolic compound which prevents cancer, heart and vascular diseases in human. In this research, the compound was extracted from leaf and fruit tissues of two Iranian grape cultivars (Rajabie Sefide Shiraz and Keshmeshie Ghermez) using a general extraction method with some modification, and analyzed quantitatively by High Performance Liquid Chromatography (HPLC). Resveratrol content in leaf and fruit of Rajabie Sefide Shiraz cultivar was higher than Keshmeshie Ghermez and its amount in the fruits of both cultivars was observed to be greater than their leaves. The accumulation of Resveratrol at the early stage of fruit growth (4 weeks after full bloom) was higher than the subsequent stages of fruit growth and development (8 weeks after full bloom and ripened fruit) in both cultivars. This compound gradually decreased from the beginning of fruit set to the complete stage of growth and development and reached the lowest level when the fruits were ripe. Leaves and fruits of both cultivars were treated with methyl jasmonate (1 mM), then the rate of resveratrol was measured after treatments. It was observed that the amount of this compound was significantly increased in treated samples when compared with the control; however, the difference diminished later. The fruits of both cultivars responded better to the treatments 4 weeks after full bloom.
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