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Search Results: 1 - 10 of 59097 matches for " Eslava- Campos Carlos "
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Identification of the autotransporter Pet toxin in Proteus mirabilis strain isolated from patients with urinary tract infections  [PDF]
Luis Raúl Gutiérrez-Lucas, Guillermo Mendoza-Hernández, Bertha González-Pedrajo, Carlos Eslava-Campos, Jaime Bustos-Martínez, Teresita Sainz-Espu?es
Advances in Biological Chemistry (ABC) , 2012, DOI: 10.4236/abc.2012.23036
Abstract: Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain.
Susceptibilidad antimicrobiana de aislamientos de Escherichia coli procedentes de ecosistemas dulceacuícolas
Romeu álvarez,Beatriz; Salazar Jiménez,Paloma; Lugo Moya,Daysi; Rojas Hernández,Nidia M; Eslava Campos,Carlos A;
Revista Cubana de Medicina Tropical , 2012,
Abstract: introduction: antimicrobial resistance is one of the biggest problems facing global public health. the emergence of resistant clinical and environmental strains worsens the situation. among the microorganisms with antimicrobial resistance, escherichia coli species stands out due to its dual role as fecal contamination indicator and pathogen. objectives: to isolate and identify escherichia coli isolates from water samples from polluted rivers located in la habana, and to determine their antimicrobial in vitro susceptibility. methods: one hundred thirteen isolates of coliform bacteria isolated from 10 sampling stations in the capital′s urban areas near almendares, quibú and luyanó rivers were studied in the period of february 2008 to june 2010. the identification of isolates, the determination of antimicrobial susceptibility and the search for extended-spectrum b-lactamase were all performed using vitek automated method. results: one hundred thirteen environmental strains of escherichia coli were identified. it showed that 23 % of the isolates were resistant to at least one of the tested antimicrobials. the highest percentages of resistance were observed to ampicilline, sulfamethoxazole-trimethoprim and ciprofloxacin. conclusions: the presence of e. coli isolates with multiple antimicrobial resistances in these rivers clearly indicates the biological risk involving the use of their waters.
Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern
Isaac-Márquez, Angélica P;Lezama-Dávila, Claudio M;Eslava- Campos, Carlos;Navarro-Oca?a, Armando;Cravioto-Quintana, Alejandro;
Memórias do Instituto Oswaldo Cruz , 1998, DOI: 10.1590/S0074-02761998000100004
Abstract: the presence of vibrio cholerae non-o1 in water supplies for human consumption in the city of campeche and rural locality of bécal was investigated. v. cholerae non-o1 was detected in 5.9% of the samples obtained in deep pools of campeche. studies conducted in bécal and neighbourhood of morelos in campeche indicated that collected samples harbored v. cholerae non-o1 in 31.5% and 8.7% respectively. there was a particular pattern of distribution of v. cholerae non-o1 serotypes among different studied regions. accordingly, v. cholerae non-o1 serotype o14 predominated in the deep pools of campeche and together with v. cholerae non-o1, o155 were preferentially founds in samples taken from intradomiciliary faucets in the neighbourhood of morelos. samples from bécal predominantly presented the serotype o112. 60% and 53.8% of all studied strains of v. cholerae non-o1 proved to be resistant to ampicillin and carbenicillin. 3.1%, 7.7% and 6.2% presented resistant to doxycycline, trimethoprim-sulfamethoxazole and erythromycin respectively. the study showed the necessity of performing a strong epidemiologic surveillance for emergence and distribution of v. cholerae non-o1
Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern
Isaac-Márquez Angélica P,Lezama-Dávila Claudio M,Eslava- Campos Carlos,Navarro-Oca?a Armando
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract: The presence of Vibrio cholerae non-O1 in water supplies for human consumption in the city of Campeche and rural locality of Bécal was investigated. V. cholerae non-O1 was detected in 5.9% of the samples obtained in deep pools of Campeche. Studies conducted in Bécal and neighbourhood of Morelos in Campeche indicated that collected samples harbored V. cholerae non-O1 in 31.5% and 8.7% respectively. There was a particular pattern of distribution of V. cholerae non-O1 serotypes among different studied regions. Accordingly, V. cholerae non-O1 serotype O14 predominated in the deep pools of Campeche and together with V. cholerae non-O1, O155 were preferentially founds in samples taken from intradomiciliary faucets in the neighbourhood of Morelos. Samples from Bécal predominantly presented the serotype O112. 60% and 53.8% of all studied strains of V. cholerae non-O1 proved to be resistant to ampicillin and carbenicillin. 3.1%, 7.7% and 6.2% presented resistant to doxycycline, trimethoprim-sulfamethoxazole and erythromycin respectively. The study showed the necessity of performing a strong epidemiologic surveillance for emergence and distribution of V. cholerae non-O1
Tensiones y confluencias. Una mirada fugaz al triple legado de los estudios histórico sociales sobre la ciencia
Eslava Juan Carlos
Revista Colombiana de Sociología , 2004,
Abstract: El artículoque aquíse presentaintenta explorar lamanera como tres grandes perspectivaspara el estudio de la ciencia han nutrido, desde su confluenciasy divergencias, lasnuevastendenciasde análisis de la cienciaque hoy en día son reconocidas bajo el nombre de estudios sociales de la ciencia. Se utiliza la noción de triple legado para darle realcea las raícesque dichos estudios tienen en la filosofía, la historia y la sociología de la ciencia. Adicionalmente, se muestra de manera muy breve como estos estudios sociales de la ciencia han adquirido notoriedad en el ámbito latinoamericanoy han renovado el interés por el estudio de la ciencia en Colombia.
La salud fragmentada en Colombia, 1910-1946. Mario Hernández álvarez Instituto de Salud Pública Universidad Nacional de Colombia, Bogotá, 2002
Eslava Juan Carlos
Revista Colombiana de Sociología , 2004,
Abstract:
Hacia una promoción de la salud al alcance de los ni os y las ni as
Juan Carlos Eslava C
Revista Facultad de Medicina de la Universidad Nacional de Colombia , 2012,
Abstract:
From the fight before death to the facing of the avoidable mortality
Juan Carlos Eslava Casta?eda
MedUNAB , 2006,
Abstract:
Serogroups, K1 antigen, and antimicrobial resistance patterns of Aeromonas spp. strains isolated from different sources in Mexico
Arteaga Garibay, Ramón I;Aguilera-Arreola, Ma Guadalupe;Navarro Oca?a, Armando;Giono Cerezo, Silvia;Sánchez Mendoza, Miroslava;Molina López, José;Eslava Campos, Carlos;Cravioto, Alejandro;Castro-Escarpulli, Graciela;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000200007
Abstract: a total of 221 strains of aeromonas species isolated in mexico from clinical (161), environmental (40), and food (20) samples were identified using the automated system biomérieux-vitek?. antisera for serogroups o1 to 044 were tested using the shimada and sakazaki scheme. the k1 antigen was examined using as antiserum the o7:k1c of escherichia coli. besides, we studied the antimicrobial patterns according to vitek automicrobic system. among the 161 clinical strains 60% were identified as a. hydrophila, 20.4% as a. caviae, and 19.25% as a. veronii biovar sobria. only a. hydrophila and a. veronii biovar sobria were found in food (55 and 90% respectively) and environmental sources (45 and 10% respectively). using "o" antisera, only 42.5% (94/221) of the strains were serologically identified, 55% (121/221) were non-typable, and 2.5% (6/221) were rough strains. twenty-two different serogroups were found, o14, o16, o19, o22, and o34 represented 60% of the serotyped strains. more than 50% of aeromonas strain examined (112/221) expressed k1 encapsulating antigen; this characteristic was predominant among aeromonas strains of clinical origin. resistance to ampicillin/sulbactam and cephazolin was detected in 100 and 67% of aeromonas strain tested for their susceptibility to antibiotics. in conclusion, antibiotic-resistant aeromonas species that possess the k1 encapsulating antigen and represent serogroups associated with clinical syndrome in man are not uncommon among aeromonas strains isolated from clinical, food and environmental sources in mexico.
CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence.
Juan Xicohtencatl-Cortes,Ariadnna Cruz-Córdova,Karina Espinosa-Mazariego,Sara A. Ochoa,Zeus Salda?a,Gerardo E. Rodea,Vicenta Cázares-Domínguez,Viridiana Rodríguez-Ramírez,Carlos A. Eslava Campos,Oscar G. Gomez-Duarte
Frontiers in Microbiology , 2014, DOI: 10.3389/fmicb.2014.00709
Abstract: Background: Enterotoxigenic Escherichia coli (ETEC) colonize the human intestinal mucosa using pili and non-pili colonization factors (CFs). CS21 (also designated Longus) is one of the most prevalent CFs encoded by a 14 kb lng DNA cluster located in a virulence plasmid of ETEC; yet limited information is available on the prevalence of CS21 positive ETEC isolates in different countries. The aim of this study was to evaluate the prevalence of CS21 among ETEC clinical isolates from Mexican and Bangladeshi children under five years old with diarrhea and to determine the phenotypic and genotypic features of these isolates. Methods: ETEC clinical isolates positive to lngA gene were characterized by genotype, multidrug-resistance, self-aggregation, biofilm formation and adherence to HT-29 cell line. Results: A collection of 303 E. coli clinical isolates were analyzed, the 81.51% (247/303) were identified as ETEC, 30.76% (76/247) were st+/lt+, and 25.10% (62/247) were positive for the lngA gene. Among the lngA+ ETECs identified, 50% of isolates (31/62) were positive for LngA protein. The most frequent serotype was O128ac:H12 found in 19.35% (12/62) of lngA+ ETEC studied. Multidrug-resistance (MDR) lngA+ ETEC isolates was identified in 65% (39/60), self-aggregation in 48.38% (30/62), and biofilm formation in 83.87% (52/62). ETEC lngA+ isolates were able to adhere to HT-29 cells at different levels. Two lngA isogenic mutants were constructed in the ETEC E9034A and ETEC73332 clinical isolate, showing a 77% and 98% reduction in adherence respectively with respect to the wild type. Conclusion: ETEC isolates that har the lngA gene showed features associated with self-aggregation, and adherence to HT-29 cells important characteristics in the human gut colonization process and pathogenesis.
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