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Search Results: 1 - 10 of 37509 matches for " Ernestina; Grimaldo-Juárez "
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Detección de geminivirus asociados a la alstroemeria (alstroemeria l.) En villa guerrero, estado de México
Cervantes-Díaz,Lourdes; Zavaleta-Mejía,Emma; Rojas-Martínez,Reina Isabel; Alanís-Martínez,Iobana; Ochoa-Martínez,Daniel Leobardo; Valadez-Moctezuma,Ernestina; Grimaldo-Juárez,Onécimo;
Interciencia , 2009,
Abstract: in alstroemeria (alstroemeria l.) plantations located in villa guerrero, mexico state, plants with symptoms similar to those induced by geminivirus in other horticultural crops have been detected. in addition, the presence of whiteflies, which are considered the most efficient vectors of these viruses, has been observed in these plantations. the goal of this work was to detect the presence of this geminivirus species in alstroemeria plants. by means of pcr analysis using primers motcp2118/motcp2123, a fragment of ~600pb similar to the amplicon obtained from phyvv-infected positive control was amplified only from symptomatic plants. nicotiana glutinosa, n. benthamiana, n. rustica, n. tabacum var. xanthi and datura stramonium plants were inoculated by bombardment with total dna obtained from symptomatic alstroemerias and positive to phyvv by means of pcr. inoculated plants showed mild mosaics and deformation of leaves, whereas in the leaves of capsicum annum plants, mosaics, vein necrosis and blisters were observed. using dna from these plants as template in pcr, amplicons corresponded to phyvv were also obtained; however, in bombarded monocotyledons, including alstroemeria, this fragment was not detected. the sequence of oligonucleotides from the pcr products showed 98% homology to phyvv geminivirus. even though symptoms presented by alstroemeria plants in the field were not reproduced, the presence of a geminivirus similar to phyvv in tissue of symptomatic plants was evidenced through pcr.
Hongos ectomicorrícicos y la tolerancia a la salinidad en plantas Ectomycorrhizal fungi and tolerance to salinity in plants
SELENE AGUILAR-AGUILAR,JESúS PéREZ-MORENO,RONALD FERRERA-CERRATO,ONéCIMO GRIMALDO-JUáREZ
Revista chilena de historia natural , 2009,
Abstract: El proceso de salinización de los suelos constituye un problema generalizado a nivel global. En este sentido, los hongos ectomicorrícicos tienen una importante participación en la recuperación de suelos forestales ya que involucran una serie de mecanismos celulares que pueden contribuir a la tolerancia a la salinidad en plantas que habitan los bosques templados o boreales. La participación de los hongos ectomicorrícicos en la tolerancia a la salinidad involucra la regulación homeostática de los iones, la mejora de captación de agua y la inducción de genes específicos en las raíces colonizadas. Los hongos ectomicorrícicos pueden estimular la presencia de osmolitos como la prolina, azúcares y polioles que contribuyen en la protección de las células vegetales. Además, estos organismos inducen la síntesis de enzimas antioxidantes y glutatión que participan en la disminución de especies reactivas de oxígeno. Esta revisión ofrece una descripción de la participación de los hongos ectomicorrícicos en la tolerancia a la salinidad en plantas. The process of salinization of the soil is a widespread problem at the global level. In this sense, ectomycorrhizal fungi have an important role in the recovery of forest soil, as it involves a number of cellular mechanisms that may contribute to the salinity tolerance in plants that inhabit temperate and boreal forests. The participation of ectomycorrhizal fungi on the salinity tolerance involves the ion-homeostasis regulation, improving uptake water and inducing specific gene in roots colonized. Likewise ectomycorrhizal fungi can stimulate the presence of osmolytes as proline, sugars and polyols that contribute to the protection of plant cells. Additionally, these organisms stimulate the synthesis of glutathione and antioxidant enzymes involved in the decrease of reactive oxygen species. This review provides an overview of participation of ectomycorrhizal fungi in the salinity tolerance in plants.
Alternativas de control en la pudrición radical de cebolla para el Valle de la Trinidad, Baja California
Pulido-Herrera, Armando;Zavaleta-Mejía, Emma;Cervantes-Díaz, Lourdes;Grimaldo-Juárez, Onécimo;
Revista mexicana de ciencias agrícolas , 2012,
Abstract: onion's root rot is the most important disease in the trinidad valle, baja california, mexico. in orderto evaluate strategies to control this disease, three experiments were conducted during 2007, 2008 and 2009. in experiment i (2007) the treatments were: 1) thiophanate methyl; 2) smicobac (trichoderma+bacillus sp. + azospirillum sp. + pseudomonas sp.); 3) cattle manure (eb); 4) waste of onion (rc); 5) clear plastic solarization (spt); 6) black plastic solarization (spn); 7) spt + eb; 8) spt + cr; 9) spn + eb; 10) spn + so; 11) trichodef (trichoderma harzianum); and 12) control. in experiment ii (2008), treatments were the same except treatment 2, replaced by a protective agent (plant extracts), and in experiment iii (2009) the treatments were: 1) spt + sheep manure (eo); 2 ) spn + eo; 3) spt; 4) spn; 5) biological control (trichoderma spp.); and 6) chemical control (2 - (thiocyanomethylthio) benzothiazole 30%. the results indicated that the clear plastic solarization treatments with or without organic amendments showed significant increases (p<0.05) from 22 to 34%yield and diameterwith respect to biological and chemical control, but the latter two treatments in the incidence and severity decreasedsignificantly (p<0.05 ), so that the clearplastic solarization and biological control, are viable alternatives to control root rot in onion crops in baja california, mexico.
Alternativas de control en la pudrición radical de cebolla para el Valle de la Trinidad, Baja California Control Alternatives for Onion root rot in the Trinity Valley, Baja California
Armando Pulido-Herrera,Emma Zavaleta-Mejía,Lourdes Cervantes-Díaz,Onécimo Grimaldo-Juárez
Revista mexicana de ciencias agrícolas , 2012,
Abstract: La pudrición radical en cebolla es la enfermedad más importante en el Valle de la Trinidad, Baja California, México. Con el propósito de evaluar estrategias de control para la enfermedad se realizaron tres experimentos durante 2007, 2008 y 2009. En el experimento I (2007) los tratamientos fueron: 1) Tiofanato metílico; 2) Smicobac (Trichoderma + Bacillus sp. + Azospirillum sp. + Pseudomonas sp.); 3) estiércol de bovino (EB); 4) residuos de cebolla (RC); 5) solarización plástico transparente (SPT), 6) solarización plástico negro (SPN); 7) SPT + EB; 8) SPT + RC; 9) SPN + EB; 10) SPN + RC; 11) Trichodef (Trichoderma harzianum);y 12)testigo. En el experimento II (2008), los tratamientos fueron los mismos excepto el tratamiento 2, sustituido porprotector (extractos vegetales); y en el experimento III (2009) los tratamientos fueron: 1) SPT + estiércol de ovino (EO); 2) SPN + EO; 3) SPT; 4) SPN; 5) control biológico (Trichoderma spp.); y 6) control químico (2- (tiocianometiltio) benzotiazol 30%. Los resultados indicaron que los tratamientos solarización plástico transparente con o sin enmiendas orgánicas, presentaron incrementos significativos (p≤ 0.05) de 22 a 34% en el rendimiento y diámetro del bulbo con respecto al control biológico y químico; sin embargo, en estos dos últimos tratamientos la incidencia y severidad se redujo significativamente (p≤ 0.05), por lo que la solarización con plástico transparente y el control biológico, son alternativas viables para el control de la pudrición radical en cultivos de cebolla en Baja California, México. Onion's root rot is the most important disease in the Trinidad Valle, Baja California, Mexico. In orderto evaluate strategies to control this disease, three experiments were conducted during 2007, 2008 and 2009. In experiment I (2007) the treatments were: 1) Thiophanate methyl; 2) Smicobac (Trichoderma+Bacillus sp. + Azospirillum sp. + Pseudomonas sp.); 3) cattle manure (EB); 4) waste of onion (RC); 5) clear plastic solarization (SPT); 6) black plastic solarization (SPN); 7) SPT + EB; 8) SPT + CR; 9) SPN + EB; 10) SPN + SO; 11) Trichodef (Trichoderma harzianum); and 12) control. In Experiment II (2008), treatments were the same except treatment 2, replaced by a protective agent (plant extracts), and in experiment III (2009) the treatments were: 1) SPT + sheep manure (EO); 2 ) SPN + EO; 3) SPT; 4) SPN; 5) biological control (Trichoderma spp.); and 6) chemical control (2 - (thiocyanomethylthio) benzothiazole 30%. the results indicated that the clear plastic solarization treatments with or without organic amendments sho
Hongos ectomicorrícicos y la tolerancia a la salinidad en plantas
AGUILAR-AGUILAR,SELENE; PéREZ-MORENO,JESúS; FERRERA-CERRATO,RONALD; GRIMALDO-JUáREZ,ONéCIMO; CERVANTES-DíAZ,LOURDES; GONZáLEZ-MENDOZA,DANIEL;
Revista chilena de historia natural , 2009, DOI: 10.4067/S0716-078X2009000100011
Abstract: the process of salinization of the soil is a widespread problem at the global level. in this sense, ectomycorrhizal fungi have an important role in the recovery of forest soil, as it involves a number of cellular mechanisms that may contribute to the salinity tolerance in plants that inhabit temperate and boreal forests. the participation of ectomycorrhizal fungi on the salinity tolerance involves the ion-homeostasis regulation, improving uptake water and inducing specific gene in roots colonized. likewise ectomycorrhizal fungi can stimulate the presence of osmolytes as proline, sugars and polyols that contribute to the protection of plant cells. additionally, these organisms stimulate the synthesis of glutathione and antioxidant enzymes involved in the decrease of reactive oxygen species. this review provides an overview of participation of ectomycorrhizal fungi in the salinity tolerance in plants.
Los elementos potencialmente tóxicos en las plantas de manglar: una revisión de los mecanismos de tolerancia involucrados
González-Mendoza,Daniel; Grimaldo Juárez,Onécimo; Cervantes Díaz,Lourdes;
Interciencia , 2008,
Abstract: mangroves have developed through evolution a number of potential mechanisms at the cellular level that let them settle in environments with high concentrations of metals. externally, they involve the participation of rhizosphere microorganisms, the formation of iron plaques and changes in their roots. internally, they involve the participation of radical exudates, metallothioneins, and phytochelatins. the comprehension of metal tolerance mechanisms by mangroves is a key factor in understanding of the physiology of these plants of great significance to ecology and biotechnology. this paper describes the mechanisms of tolerance to potentially toxic elements present in some species of mangroves.
Effect of Nicotine on Dopamine and Glutathione Levels in Presence of Oligoelements in Brain Regions of Young Rats——Effect of Nicotine on Brain Regions of Rat  [PDF]
David Calderón Guzmán, Ernestina Hernández García, Francisca Trujillo Jiménez, Gerardo Barragán Mejía, Hugo Juárez Olguín, José A. Saldivar González, Daniel Santamaria del Angel, Norma Osnaya Brizuela
Neuroscience & Medicine (NM) , 2012, DOI: 10.4236/nm.2012.33032
Abstract: Aim: The purpose of this study was to understand the mechanism of nicotine mediated addiction and the role of oligoelements in reducing its effect. Methods: Male Wistar rats (weight 80 g) were treated with single and repeated doses of nicotine and/or oligoelements as follows: group 1 (control) NaCl 0.9%; group 2, nicotine (1 mg/kg); group 3, oligoelements (50 μl/rat); and group 4, nicotine (1 mg/kg) + oligoelements (50 μl/rat). All drugs were intraperitoneally administered for 4 days. Blood for the measurement of glucose was obtained from all the animals. Samples of the brain regions (cortex, hemispheres and cerebellum + medulla oblongata) of each rat were obtained and used to measure the concentrations of dopamine, GSH levels, and lipid peroxidation (TBARS) using fluorescence and spectrophotometric methods. Results: Glucose level increased in rats treated with nicotine and oligoelements (p < 0.05), while GSH level decreased in cerebellum/medulla oblongata and hemispheres (p < 0.05) of the same animals. TBARS levels increased in cerebellum/medulla oblongata and hemispheres of animals treated with nicotine and oligoelements, but decreased in the same regions (p < 0.05) in rats treated only with oligoelements. The levels of dopamine decreased in cortex and hemispheres, but increased in cerebellum/medulla and oblongata regions of rats treated with both compounds (p < 0.05). Conclusions: Nicotine and oligoelements are associated with increase in the level of glucose, an effect that was more pronounced in the group treated with both drugs. Reduction of oxidative stress and dopamine metabolism may be involved in this effect.
Dinámica poblacional de la lombriz Eisenia foetida en estiércol composteado y fresco de bovino y ovino (Dynamics population earthworm Eisenia foetida in fresh and composted manure of bovine and ovine)
Gutiérrez Vázquez Ernestina,Juárez Caratachea Aureliano,Mondragón Ancelmo Jaime | Rojas Sandoval A. Luis:
REDVET , 2007,
Abstract: Se evaluó la dinámica poblacional de la lombriz Eisenia foetida, alimentada con estiércol fresco y composteado de bovinos, alimentados a base de (rastrojo molido y bloques multinutricionales) y de ovinos, alimentados con (silo de maíz y concentrado). Los tratamientos fueron: T1. Estiércol composteado de bovino (ECB), T2. Estiércol composteado de ovino (ECO), T3. Estiércol fresco de bovino (EFB) y T4. Estiércol fresco de ovino (EFO), en un dise o completamente al azar con cuatro repeticiones. Las variables de respuesta fueron: temperatura, pH, número de cocones, número y biomasa total de lombrices adultos y jóvenes. En los estiércoles frescos, en los primeros tres días, las lombrices murieron (P< 0.05). En el estiércol composteado de bovino fue en donde se presentaron mayor número de cocones (201.75±94.46), lombrices jóvenes (3.25±2.50) y la viabilidad de lombrices adultos (45.25±15.19), en comparación con el estiércol composteado de ovino que presentó menor número de cocones (89.0±11.74), no presentó ninguna lombriz joven, sin embargo, tuvo mayor viabilidad de lombrices adultos (81.75±16.47). Los resultados indicaron que los estiércoles composteados son los mejores en cuanto a la dinámica poblacional de las lombrices, en comparación con los estiércoles frescos.
Detección de mutaciones relacionadas con resistencia a herbicida en avena silvestre (Avena Fatua L.)
Tovar Hernández, Homero;Cruz Villegas, Manuel;Ponce Medina, Francisco;Mu?iz Salazar, Raquel;Avenda?o Reyes, Leonel;Grimaldo Juárez, Onésimo;Medina Basulto, Gerardo;
Agricultura técnica en México , 2008,
Abstract: two oligonucleotid groups were evaluated in order to detect two punctual mutations of the gene acetyl-coa carboxilase (accasa) in avena fatua l., which are related to the resistance to herbicides that inhibit fatty acid synthesis. four populations of wild oat, two resistant (m5r and m28r) and two susceptible (m25s and m43s), to field application of clodinafop propargil were selected. two groups of oligonucleotids were developed based on the sequence of the gene of the accasa from wild oat (genebank access number af231334). the first group, vse11gm, vre11rgm, acvii 11gm and acvii 11rgm, was designed to detect the change of timine (t) by adenine (a) in position 1 322 of the described gene and which originated the change of isoleucine (att) by asparagine (aat). the second group of oligonucleotids, vrdicgm, vrditrgm, acvrg1gm and acvrg1rgm, was designed to detect the change of citosine or timine (t or c) by adenine (a) in position 541 of the same gene, and which originated the change of isoleucine (ata) by leucine (cta or tta). consequently, it was not possible to differentiate the populations that offered resistance in field from those which were susceptible and which would cause the change of isoleucine by leucine in the position 541 of the gene for both mutations. the absence of amplification of the fragment of 480 pb for the resistant population m5r, with the first group of oligonucleotids, as well as the absence of the amplification of the fragments 496 pb and 330 pb in the four populations with the second group of oligonucleotids, indicated the probability that it is a different substitution the one that confers resistance to the wild oat toward this group of herbicides.
Respuesta fisiológica de Euglena gracilis al estrés por cobre
García, David Cervantes;González-Mendoza, Daniel;Cervantes-Díaz, Lourdes;Trejo, Adriana Morales;Juárez, Onecimo Grimaldo;Zamora-Bustillos, Roberto;
Química Nova , 2011, DOI: 10.1590/S0100-40422011000700020
Abstract: the objective of this study was to evaluate the toxic effect of cu2 + in the physiological development of e. gracilis. the results showed that e. gracilis had an effect on the dose-dependent growth to the concentration of metal. the exposure of e. gracilis metal at doses of 0.8 and 1.6 mm of cu2+ showed a significant negative effect on the stability of dna and photosynthetic pigments involved in capturing light in the antenna complex after 144 h of exposure to the metal.
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