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Search Results: 1 - 10 of 275 matches for " Erel Ozcan "
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Estado oxidante/antioxidante total em recém-nascidos ictéricos antes e depois da fototerapia
Aycicek, Ali;Erel, Ozcan;
Jornal de Pediatria , 2007, DOI: 10.1590/S0021-75572007000500006
Abstract: objective: to assess the effect of phototherapy on serum oxidant and antioxidant status in hyperbilirubinemic full-term newborns. method: thirty-four full-term infants from 3 to 10 days of age exposed to phototherapy were studied. the serum antioxidant status was assessed by measuring the total antioxidant capacity (tac) and individual antioxidant components: vitamin c, uric acid, albumin, thiol contents and total bilirubin. the oxidant status was assessed by determining the total oxidant status (tos), oxidative stress index (osi) and individual oxidant components: malondialdehyde (mda), and lipid hydroperoxide levels. results: vitamin c, uric acid, total bilirubin and mda concentration were significantly lower, whereas serum tos, lipid hydroperoxide and osi levels were significantly higher after phototherapy (p < 0.05). there were significant positive correlations between serum total bilirubin and mda (r = 0.434, p = 0.001). conclusions: although the mda level was reduced after phototherapy, phototherapy has a negative impact on numerous parts of the oxidant/antioxidant defense system in jaundiced full-term newborns, exposing them to potential oxidative stress.
Dynamic Thiol-Disulphide Homeostasis in Patients with Multiple Sclerosis  [PDF]
Gonul Vural, Sadiye Gumusyayla, Hesna Bektas, Orhan Deniz, Merve Ergin, Ozcan Erel
World Journal of Neuroscience (WJNS) , 2016, DOI: 10.4236/wjns.2016.63026
Abstract: Objective: The aim of this study was to evaluate thiol-disulphide homeostasis in patients with Multiple Sclerosis (MS) during attack and remission. Design and Methods: A total of 82 patients admitted to our clinic were divided into 2 groups according to whether they are having an attack or not. Peripheral blood samples were taken from all patients and their native thiol, disulphide and total thiol concentrations were measured by a newly developed automated method. In addition, their disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios were calculated. Results: The average disulphide values, disulphide/native thiol and disulphide/total thiol ratios of the patients with MS who were having an attack were found to be significantly higher compared to those in remission (p = 0.001, p = 0.001, p = 0.001, respectively). Conclusion: Our results have shown that thiol-disulphide balance was shifted to the oxidative area during the relapse. These homeostasis parameters, which can be easily measured by this newly developed method at low costs, may be important in terms of showing the disease activity and presenting the underlying mechanisms of the disease.
Fototerapia causa danos ao DNA de leucócitos mononucleares periféricos em recém-nascidos a termo
Aycicek, Ali;Kocyigit, Abdurrahim;Erel, Ozcan;Senturk, Hakan;
Jornal de Pediatria , 2008, DOI: 10.1590/S0021-75572008000200009
Abstract: objective: our aim was to determine whether endogenous mononuclear leukocyte dna strand is a target of phototherapy. methods: the study included 65 term infants aged between 3-10 days that had been exposed to intensive (n = 23) or conventional (n = 23) phototherapy for at least 48 hours due to neonatal jaundice, and a control group (n = 19). dna damage was assayed by single-cell alkaline gel electrophoresis (comet assay). plasma total antioxidant capacity and total oxidant status levels were also measured, and correlation between dna damage and oxidative stress was investigated. results: mean values of dna damage scores in both the intensive and conventional phototherapy groups were significantly higher than those in the control group (p < 0.001). mean values and standard deviation were 32 (9), 28 (9), 21 (7) arbitrary unit, respectively. total oxidant status levels in both the intensive and conventional phototherapy groups were significantly higher than those in the control group (p = 0.005). mean (standard deviation) values were 18.1 (4.2), 16.9 (4.4), 13.5 (4.2) μmol h2o2 equivalent/l, respectively. similarly, oxidative stress index levels in both the intensive and conventional phototherapy groups were significantly higher than those in the control group (p = 0.041). plasma total antioxidant capacity and total bilirubin levels did not differ between the groups (p > 0.05). there were no significant correlations between dna damage scores and bilirubin, total oxidant status and oxidative stress levels in either phototherapy group (p > 0.05). conclusions: both conventional phototherapy and intensive phototherapy cause endogenous mononuclear leukocyte dna damage in jaundiced term infants.
Diagnostic performance characteristics of rapid dipstick test for Plasmodium vivax malaria
Aslan, Gonul;Ulukanligil, Mustafa;Seyrek, Adnan;Erel, Ozcan;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000500018
Abstract: we compared the diagnostic performance characteristics of newly developed method, the rapid dipstick test, which provides colorimetric determination by developing antibody to the lactate dehydrogenase enzyme of parasites, with conventional standard thick-blood film examination. for the rapid test, optimal commercial kits were used. the results were also evaluated with clinical findings from patients. the parasites were determined by microscopic examination of thick-blood films from 81 patients with vivax malaria from southeastern anatolia, turkey. the optimal test results were found to be negative in five patients who were diagnosed clinically and through thick-film testing as having vivax malaria. there was no false positivity observed with the optimal test. we concluded that this rapid malaria test has a lower level of sensitivity than the classical thick-blood-film test for malaria, but that these methods have equal specificity.
Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis
Erel, Ozcan;Kocyigit, Abdurrahim;Bulut, Vedat;Gurel, Mehmet Salih;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000200009
Abstract: the metabolisms of reactive nitrogen and oxygen intermediates (rni and roi) in patients with cutaneous leishmaniasis (cl) were investigated and compared with those of healthy subjects. to determine rni metabolism, nitrite plus nitrate concentrations were measured spectrophotometrically. nitrite concentration in plasma was determined directly by the griess method. nitrate levels in plasma were measured after reduction into nitrite by using copper-cadmium-zinc. roi metabolism was evaluated by measuring erythrocyte superoxide dismutase, catalase and glutathione peroxidase activities. plasma nitrite plus nitrate levels and erythrocyte superoxide dismutase activity were higher in the patient group than healthy subjects (p<0.01). in contrast, erythrocyte catalase and glutathione peroxidase activities were lower (p<0.05, p<0.01, respectively). roi metabolism was altered in relation to hydrogen peroxide elevation in patients with cl. these alterations in roi enable nitric oxide (no) to amplify its leishmanicidal effect. the determination of roi and rni in patients with cl may be a useful tool to evaluate effector mechanisms of no and clinical manifestations.
Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis
Erel Ozcan,Kocyigit Abdurrahim,Bulut Vedat,Gurel Mehmet Salih
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: The metabolisms of reactive nitrogen and oxygen intermediates (RNI and ROI) in patients with cutaneous leishmaniasis (CL) were investigated and compared with those of healthy subjects. To determine RNI metabolism, nitrite plus nitrate concentrations were measured spectrophotometrically. Nitrite concentration in plasma was determined directly by the Griess method. Nitrate levels in plasma were measured after reduction into nitrite by using copper-cadmium-zinc. ROI metabolism was evaluated by measuring erythrocyte superoxide dismutase, catalase and glutathione peroxidase activities. Plasma nitrite plus nitrate levels and erythrocyte superoxide dismutase activity were higher in the patient group than healthy subjects (p<0.01). In contrast, erythrocyte catalase and glutathione peroxidase activities were lower (p<0.05, p<0.01, respectively). ROI metabolism was altered in relation to hydrogen peroxide elevation in patients with CL. These alterations in ROI enable nitric oxide (NO) to amplify its leishmanicidal effect. The determination of ROI and RNI in patients with CL may be a useful tool to evaluate effector mechanisms of NO and clinical manifestations.
Diagnostic performance characteristics of rapid dipstick test for Plasmodium vivax malaria
Aslan Gonul,Ulukanligil Mustafa,Seyrek Adnan,Erel Ozcan
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: We compared the diagnostic performance characteristics of newly developed method, the rapid dipstick test, which provides colorimetric determination by developing antibody to the lactate dehydrogenase enzyme of parasites, with conventional standard thick-blood film examination. For the rapid test, OptiMAL commercial kits were used. The results were also evaluated with clinical findings from patients. The parasites were determined by microscopic examination of thick-blood films from 81 patients with vivax malaria from southeastern Anatolia, Turkey. The OptiMAL test results were found to be negative in five patients who were diagnosed clinically and through thick-film testing as having vivax malaria. There was no false positivity observed with the OptiMAL test. We concluded that this rapid malaria test has a lower level of sensitivity than the classical thick-blood-film test for malaria, but that these methods have equal specificity.
Adenosine Deaminase and Guanosine Deaminase Activities in Sera of Patients with Viral Hepatitis
Kalkan Ahmet,Bulut Vedat,Erel Ozcan,Avci Senel
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: In order to investigate purin and primidin metabolism pathways in hepatitis, adenosine deaminase (ADA) and guanosine deaminase (GDA) activities in sera of patients with different types and manifestations of viral hepatitis disease (A, B, C, D, E, chronic, acute) were investigated and compared with the control group of healthy individuals. Hepatitis cases were classified with respect to their serological findings and clinics. When compared all the hepatitis cases with the controls, levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzymes, as well as ADA and GDA, were significantly higher than the control group (p<0.01). Levels of ADA and GDA in hepatitis cases were determined as 26.07±11.98 IU/l and 2.37±1.91 IU/l, respectively. When compared their ADA and GDA levels amongst the classified hepatitis groups, there was no difference in ADA levels amongst cases (p>0.05). However, GDA levels in hepatitis A group were closed to the controls. Increase in serum ADA activities in hepatitis forms may be dependent on and reflect the increase in phagocytic activity of macrophages and maturation of T-lymphocytes, and may be valuable in monitoring in viral hepatitis cases.
Adenosine Deaminase Activities in Sera, Lymphocytes and Granulocytes in Patients with Cutaneous Leishmaniasis
Erel Ozcan,Kocyigit Abdurrahim,Gurel Mehmet Salih,Bulut Vedat
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract: Adenosine deaminase (ADA) activities in sera, lymphocytes and granulocytes in patients with cutaneous leishmaniasis were investigated and compared with control groups. Fifty patients and 50 healthy individuals were studied. The clinical diagnosis was parasitologically confirmed by culture and Giemsa stain. ADA activities were measured by colorimetric method. Serum ADA activities 37.80 ± 11.90, 18.28 ± 6.08 IU/L (p<0.0001), lymphocyte specific ADA activities 14.90 ± 7.42, 8.38 ± 7.42 U/mg protein (p = 0.04), granulocyte specific ADA activities 1.15 ± 0.73 , 1.09 ± 0.67 U/mg protein ( p>0.05) were found in patients and control groups, respectively. ADA activity increases in some infectious diseases were cell mediated immune mechanisms are dominant. In cutaneous leishmaniasis, lymphokine-mediated macrophage activity is the main effector mechanism. Increase in serum and lymphocyte ADA activities in patients with cutaneous leishmaniasis may be dependent on and reflects the increase in phagocytic activity of macrophages and maturation of T-lymphocytes.
Antioxidant and Oxidant Levels of Pepper (Capsicum annuum cv. ‘Charlee’) Infected with Pepper Mild Mottle Virus
Murat DIKILITAS,Mehmet Ertugrul GULDUR,Ahmet DERYAOGLU,Ozcan EREL
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2011,
Abstract: Total antioxidant status (TAS), total oxidant status (TOS), as well as total phenol (TP), free phenol (FP), conjugated phenol (CP), and the vitamin C content of pepper plants (Capsicum annuum cv. ‘Charlee’) infected with Pepper mild mottle virus (PMMoV) were determined via a novel automated antioxidant system using test kits. PMMoV was first identified in Turkey in 1994 in commercial pepper (Capsicum annuum cv. ‘Charlee’) fields and caused an outbreak in southern Turkey by 2006, especially in greenhouses. Infected plants were confirmed by ELISA using commercial kits for the virus. The infected plants had stunted growth; and leaves and fruits became mottled, puckered, malformed, deformed, and marked by off-colored sunken areas. Fruits expressing virus symptoms were utilized for biochemical analyses. The percent ratio of TOS to TAS was calculated as the oxidative stress index (OSI). The TAS level and the vitamin C content were found to be significantly lower in infected peppers as compared to healthy peppers. However, TOS, OSI, TP, FP, and CP levels were significantly higher in infected pepper fruits than in healthy ones. In this study, it has been demonstrated that low levels of TAS and high levels of TOS, as well as high OSI status, reflects the quality of peppers. Using this method, the quality of fruits and the condition of plants can be determined in advance of stress development.
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