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First isolation of enterovirus 71 (EV-71) from Northern Brazil
Lamar?o, Leticia Martins;Maciel, Ana Maria T. R.;Gomes, Maria de Lourdes Contente;
Brazilian Journal of Infectious Diseases , 2003, DOI: 10.1590/S1413-86702003000400009
Abstract: enterovirus 71 (ev-71) has been associated to cases of neurological disease in many countries including brazil. this virus has now been reported from three of the five brazilian regions. our study relates the findings concerning to the first isolate of this virus in northern region of brazil. a 15-month old female patient, from the rural zone of the municipality of santana do araguaia in southern pará state was admitted at the hospital with acute, flaccid, asymmetric and ascending motor deficiency, located in the right lower limb. stools samples from this child were inoculated in rd cells and was isolated an ev-71. we plan to sequence our strain and compare it to other isolates in brazil. differences at the molecular level can explain why ev-71 strains circulating in other continents, such as asia, appear to be more virulent.
First isolation of enterovirus 71 (EV-71) from Northern Brazil
Lamar?o Leticia Martins,Maciel Ana Maria T. R.,Gomes Maria de Lourdes Contente
Brazilian Journal of Infectious Diseases , 2003,
Abstract: Enterovirus 71 (EV-71) has been associated to cases of neurological disease in many countries including Brazil. This virus has now been reported from three of the five Brazilian regions. Our study relates the findings concerning to the first isolate of this virus in Northern region of Brazil. A 15-month old female patient, from the rural zone of the municipality of Santana do Araguaia in southern Pará state was admitted at the hospital with acute, flaccid, asymmetric and ascending motor deficiency, located in the right lower limb. Stools samples from this child were inoculated in RD cells and was isolated an EV-71. We plan to sequence our strain and compare it to other isolates in Brazil. Differences at the molecular level can explain why EV-71 strains circulating in other continents, such as Asia, appear to be more virulent.
Gendrowahyuhono Gendrowahyuhono,Suharyono Wuryadi
Bulletin of Health Research , 2012,
Abstract: Telah dilakukan survey enterovirus infection di antara anak-anak di bawah umur 5 tahun di Purwa-karta. Isolasi virus dari 404 specimen berhasil mendapatkan 66 (16,3 %) enterovirus strain. Dari 66 entero-virus strain tersebut, virus Coxsackie B-5 merupakan yang terbanyak didapatkan, dan frekwensinya pa-ling tinggi pada bulan Juli 1978, yang kemudian hampir menghilang pada bulan-bulan berikutnya. Le-bih dari 50 % anak-anak yang berumur satu tahun, menunjukkan serologis positip terhadap virus polio type-1 dan type-2, sedangkan serologis positip terhadap virus polio type-3, pada umur yang sama, hanya 25 %. Proporsi dari triple negatip menurun dengan bertambahnya umur, akan tetapi 30 % dari anak-anak dibawah umur satu tahun dan 14 % dari anak-anak umur 1-2 tahun tidak mempunyai antibody terha-dap masing-masing ketiga jenis virus polio. Dari penelitian ini dapat disimpulkan bahwa vaksinasi polio paling baik dilaksanakan pada bulan antara September sampai dengan Maret, dan diberikan pada anak-anak di bawah umur 2 tahun.
Chronic Enterovirus D68 Bronchiolitis Causing Severe Respiratory Insufficiency  [PDF]
John Chia, Andrew Chia, David Wang, Rabiha El-Habbal, Deren Sinkowitz
Open Journal of Respiratory Diseases (OJRD) , 2016, DOI: 10.4236/ojrd.2016.63007
Abstract: Human enteroviruses are less well-known causes of acute bronchiolitis. In recent years, Enterovirus D68 [EV D68] has emerged as significant cause of epidemic viral bronchiolitis and pneumonia in the United States and other countries. Chronic bronchiolitis has not been previously attributed to EV D68. We documented EV D68 in open lung biopsies of a young adult patient who was frequently admitted to the hospital for severe exacerbation of respiratory infections and subsequently developed progressive respiratory insufficiency. The difficulty of diagnosis and potential economic impact of this illness is discussed.
Mycophenolate mofetil inhibits the development of Coxsackie B3-virus-induced myocarditis in mice
Elizaveta Padalko, Erik Verbeken, Patrick Matthys, Joeri L Aerts, Erik De Clercq, Johan Neyts
BMC Microbiology , 2003, DOI: 10.1186/1471-2180-3-25
Abstract: Four week old C3H-mice were infected with CBV3 and received twice daily, for 7 consecutive days (from one day before to 5 days post-virus inoculation) treatment with MMF via oral gavage. Treatment with MMF resulted in a significant reduction in the development of CBV-induced myocarditis as assessed by morphometric analysis, i.e. 78% reduction when MMF was administered at 300 mg/kg/day (p < 0.001), 65% reduction at 200 mg/kg/day (p < 0.001), and 52% reduction at 100 mg/kg/day (p = 0.001). The beneficial effect could not be ascribed to inhibition of viral replication since titers of infectious virus and viral RNA in heart tissue were increased in MMF-treated animals as compared to untreated animals.The immunosuppressive agent MMF results in an important reduction of CBV3-induced myocarditis in a murine model.Viral myocarditis is a common pathological condition detected in approximately 1% of unselected asymptomatic individuals [1]. Many viruses have been shown to be involved as causative agents, but the principal agents belong to enteroviruses in general and coxsackieviruses in particular [2,3]. The proportion of cases of myocarditis with coxsackieviral ethiology varies but can in 25–50% of the cases be attributed to coxsackie B group viruses (CBV) [4,5]. Although most enterovirus-related cardiac illnesses are subclinical, severe arrythmias and sudden cardiac death may appear. About 10 to 20% of symptomatic patients will eventually develop chronic cardiac disease, which may progress to dilated cardiomyopathy, a severe pathological condition, often requiring heart transplantation [6-8]. Overall, the enteroviral genome is present in the hearts of 15–20% of patients with dilated cardiomyopathy [4,5,9,10]. Both direct viral injury and the immune response of the host are believed to play a role in the pathogenesis of viral heart disease [11]. Recent studies in mouse models show that direct viral-induced damage to the heart is necessary to cause CBV3-induced myocarditis [12
Echovirus 30 associated with cases of aseptic meningitis in state of Pará, Northern Brazil
Castro, Ceyla Maria Oeiras de;Oliveira, Darleise S;Macedo, Olinda;Lima, Maria José L;Santana, Marquete B;Wanzeller, Ana Lucia Monteiro;Silveira, Edna da;Gomes, Maria de Lourdes Contente;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000300008
Abstract: investigation of the aetiology of viral meningitis in brazil is most often restricted to cases that occur in the southern and southeastern regions; therefore, the purpose of this study is to describe the viral meningitis cases that occurred in state of pará, northern brazil, from january 2005-december 2006. the detection of enterovirus (ev) in cerebrospinal fluid was performed using cell culture techniques, rt-pcr, nested pcr and nucleotide sequencing. the ages of the 91 patients ranged from < one year old to > 60 years old (median age 15.90 years). fever (87.1%), headache (77.0%), vomiting (61.5%) and stiffness (61.5%) were the most frequent symptoms. of 91 samples analyzed, 18 (19.8%) were positive for ev. twelve were detected only by rt- pcr followed by nested pcr, whereas six were found by both cell culture and rt-pcr. from the last group, five were sequenced and classified as echovirus 30 (echo 30). phylogenetic analyses revealed that echo 30 detected in northern brazil clustered within a unique group with a bootstrap value of 100% and could constitute a new subgroup (4c) according to the phylogenetic tree described by oberste et al. (1999). this study described the first molecular characterization of echo 30 in brazil and this will certainly contribute to future molecular analyses involving strains detected in other regions of brazil.
Hepatitis y coagulopatía secundaria a infección neonatal por enterovirus
Pérez,Ana C.; Soler Vigil,Virginia; Giannivelli,Silvina; López Guinea,Alejandra; Pérez-Piaya Moreno,Rosa;
Archivos argentinos de pediatr?-a , 2009,
Abstract: enteroviruses cause a wide spectrum of clinical manifestations in neonates, from asymptomatic infection to severe and fulminant, sometimes fatal disease. a septic disease is the clinical manifestation of one fourth of the cases in neonates, with multiorganic affection. hepatitis and coagulopathy are related to bad prognosis, generally associated to catastrophic hemorrhages. in those cases, an aggressive support therapy and correct administration of platelet and clotting factor replacement is necessary. the liver, in survivors, frequently has sufficient regenerative capacity to allow normalization of hepatic function and adequate growth of the child. we present the case of a newborn who developed hepatitis and coagulopathy secondary to an enteroviral infection, with total recovery and absence of hemorrhagic complication. her mother had a febrile syndrome 24 hours ante partum.
Miocarditis aguda primaria. Experiencia de diez a?os en el Instituto Nacional de Cardiología "Ignacio Chávez"
Guillen-Ortega, Fernando;Soto, María Elena;Reyes, Pedro A;
Archivos de cardiología de México , 2005,
Abstract: acute myocarditis (am) is associated with viral infections: coxsackie and echoviruses among others. autoimmunity has been proposed as a pathogenic mechanism. benefit of classic immunosuppression (prednisone-azathioprine) or immunomodulation (monomeric-human igg) is still uncertain. objective: to review incidence and clinical approach to am at a cardiology referral center. material and methods: a 10-yeard period (1992-2003) is reviewed. a standard ques-tionary was applied to 49 consecutive patients referred by clinicians with a diagnosis of am. results: am was found in 17 women and 32 men, median age 24 and 28 years, respectively. they presented heart failure with dyspnea/ortop-nea (70-47%), peripheral edema/jugular vein plethora (41-37%), chest pain, and tachycardia (50%), nyha functional class was iii-iv in 22. the ekg showed sinus tachycardia or conduction defects. transthoracic echocardiograms in 47 cases showed ef (mean) of 41% with enlarged left ventricle diameter. antivirus antibodies were present in 54% of those cases studied, coxsackie or echovirus were identified through a serologic assay. twenty-nine (61%) of our cases developed dilated cardiomyopathy, three patients died. it is not possible to reach a conclusion regard to immunomodulation therapy, because it was applied to only 12 patients. conclusions: at the instituto nacional de cardiología "i. chávez", am depicts an incidence of 1/1,000 patients a year. it is necessary to standardize the clinical approach for diagnosis and treatment, progression to dilated cardiomyopathy and deadth during acute stage occurs in two-thirds of our patients
Análisis de un brote de meningitis viral en la provincia de Tucumán, Argentina
Freire,María Cecilia; Cisterna,Daniel Marcelo; Rivero,Karina; Fabián Palacios,Gustavo; Casas,Inmaculada; Tenorio,Antonio; Gómez,Jorge Alberto;
Revista Panamericana de Salud Pública , 2003, DOI: 10.1590/S1020-49892003000300007
Abstract: objective: to confirm the occurrence of an outbreak of viral meningitis in 1996 in the province of tucuman, argentina, and to study the outbreak's epidemiological characteristics. methods: we analyzed information from the national epidemiological surveillance system of the ministry of health (moh) of argentina for 1994-1998 that had been provided by the moh's bureau of epidemiology. we calculated incidence rates using population estimates for the years 1994-1998 developed by the national statistics and census institute, based on the 1991 census. we studied frequencies with contingency tables, using the chi-square method with yates' correction. results were considered significant when p < 0.05. results: we confirmed the occurrence of an outbreak of 189 cases of viral meningitis between 11 february and 18 may 1996. the incidence of cases in tucuman province increased between 1995 and 1996, from 0.5 to 19.3 cases per 100 000 person-years. that 1996 rate in tucuman was significantly higher than what was seen in the rest of the country (2.8 cases per 100 000 person-years). of the 189 cases, 142 of them (75.1%) occurred in children less than 9 years old. out of 111 samples studied, 65 of them (58.6%) were enterovirus-positive. through reverse transcription-nested polymerase chain reaction, enteroviruses were found in 66.3% (53/80) of the cases studied by this method, versus in only 29.6% (24/81) of the cases studied through viral isolation. in the 22 samples that were serotyped, echovirus type 4 was identified in 15 of them (68%): 5 by isolation, 3 by sequencing, and 7 by both methods. during the tucuman outbreak, at least 56% of the cases were hospitalized. this viral meningitis outbreak shows the capacity of enteroviruses to spread and cause disease. conclusions: the use of molecular methods makes it possible to rapidly diagnose the etiological virus and to better control an outbreak. recognizing this outbreak in tucuman sooner could have averted the majority of the hos
Aplicación de la secuenciación nucleotídica de VP1 a la identificación de Enterovirus humanos
Barrios Olivera,Julio Antonio; Sarmiento Pérez,Luis; Valdés,Odalys; Mas Lago,Pedro; Palomera Puentes,Rosa;
Revista Cubana de Medicina Tropical , 2003,
Abstract: the introduction of a mollecular method to identify the entoviruses based on the amplification, sequencing and phylogenetic analysis of protein vp1 was described. it was proved that this method reduces significantly the time required for the identification of the isolated entoviruses and that it is very useful in the characterization of isolates which are difficult to typify by the routine immunoloigical reagents. as it is a very fast technqiue, its use is very important during epidemics to determine the causal agent rapidly.
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