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Search Results: 1 - 10 of 194268 matches for " Elvira; Lam Díaz "
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Caracterización fenotípica de las células madre de médula ósea utilizadas en la terapia celular regenerativa
Macías-Abraham,Consuelo; del Valle-Pérez,Lázaro O; Baganet-Cobas,Aymara; Dorticós-Balea,Elvira; Jaime-Fagundo,Juan C; Lam-Díaz,Rosa M; Socarrás-Ferrer,Bertha B; Sánchez-Segura,Miriam; Marsán-Suárez,Vianed; Palma-Salgado,Lourdes; Hernández-Ramírez,Porfirio; Ballester-Santovenia,José M;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: regenerative medicine is a novel therapeutic method with broad potential for the treatment of various illnesses, based on the use of bone marrow (bm) stem cells, whose phenotypic characterization is limited. the paper deals with the expression of different cell membrane markers in mononuclear bm cells from 14 patients who underwent autologous cell therapy, obtained by medullary puncture and mobilization to peripheral blood, with the purpose of characterizing the different types of cells present in that heterogeneous cellular population and identifying the adhesion molecules involved in their adhesion. a greater presence was observed of adherent stem cells from the marrow stroma in mononuclear cells obtained directly from the bm; a larger population of cd90+ cells in mononuclear cells from cd34-/cd45- peripheral blood with a high expression of molecules cd44 and cd62l, which suggests a greater presence of mesenchymal stem cells (msc) in mobilized cells from the marrow stroma. the higher levels of cd34+ cells in peripheral blood stem cells with a low expression of molecules cd117- and dr- suggests the presence of hematopoietic stem cells, hemangioblasts and progenitor endothelial cells mobilized to peripheral circulation. it was found that mononuclear cells from both the bm and peripheral blood show a high presence of stem cells with expression of adhesion molecule cd44 (mmc marker), probably involved in their migration, settling and differentiation.
Aislamiento de células mononucleares de sangre periférica para trasplante de células madre: Método simplificado
Cortina Rosales,Lázaro; Hernández Ramírez,Porfirio; López De Roux,Maríadel Rosario; Artaza Sanz,Heriberto M; Dorticós Balea,Elvira; Macías Abraham,Consuelo; Socarrás Ferrer,Berta B; Lam Díaz,Rosa María; González Suárez,Tania; Matamoros,María de los ángeles; González Iglesias,Ana Iris; Salgado Arocena,Odalis; Hernández Rego,Yakima;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: in the last years, the topic of stem cells has arisen an increasing interest for its therapeutic potential in diseases that have not an effective treatment so far. a prospsective and exploratory study was conducted in patients with obliterant atherosclerosis of the lower limbs to evaluate the safety and effectiveness of a manual method of collection and processing of mononuclear cells and of cd34+ cells, starting from mobilized peripheral blood. the blood was processed in closed collection systems, using hydroxyethyl starch as a potentiator of erythrocyte sedimentation. the patients were treated with granulocyte colony stimulating factor at total doses of 40μcg/kg of weight during 2 days. self-donation was performed when the leukocyte count was higher than 20 x 109/l. to assess the efficacy of the method, the amounts of nucleated cells, of mononuclear cells and of cd 34+ cells in the cellular concentrate were analyzed. cellular viability was determined and a microbiological study of the material obtained was conducted. it was proved that the method is efficient and safe, since adequate cellular levels with a high viability and absence of bacterial contamination are attained. on the other hand, it is simple and cheap, which allows its application in other health centres, particularly in those with less resources. this makes possible that more patients benefit from the stem cell treatment.
Metodología para la confección de un proyecto de investigación
Lam Díaz,Rosa María;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2005,
Abstract: research is a systematic, organized and objective process directed to answer a question that is a research problem. the basic unit of the research process is the research project that contains in detail the organization given to this activity and the way it will be carried out. that`s why, it represents a guide for the working team during the development of the research. every institution reserves itself the right to demand the project format it considers necessary according to its main interests. however, a project should have the following accurate information: title of the research, list of researchers, institution responsible for the research and its management, summary, definition and formulation of the problem, justification of the study, formulation of the hypothesis and objectives, type of study, universe and sample, operationalization of the variables, data collection plan, information processing plan, ethical considerations, resources, bibliographic references, schedule, form of dissemination of the results and annexes
Metodología para la confección de un proyecto de investigación Methodology for making a research project
Rosa María Lam Díaz
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2005,
Abstract: La investigación es un proceso sistemático, organizado y objetivo destinado a responder a una pregunta problema de investigación. La unidad básica del proceso investigativo es el proyecto de investigación, documento que recoge de manera pormenorizada la organización que se ha dado a esta actividad y la forma en que se ejecutará la misma, por lo que representa una guía para el equipo de trabajo durante el desarrollo de la investigación. Cada institución se reserva el derecho de exigir el formato de proyecto que considere de acuerdo con sus intereses fundamentales, no obstante, un proyecto debe contener la siguiente información detallada: título de la investigación, lista de investigadores, institución responsable de la investigación y su dirección, resumen, definición y formulación del problema, justificación del estudio, formulación de hipótesis y objetivos, tipo de estudio, universo y muestra, operacionalización de las variables, plan de recolección de datos, plan de procesamiento de la información, consideraciones éticas, recursos, referencias bibliográficas, cronograma, forma de divulgación de los resultados y anexos Research is a systematic, organized and objective process directed to answer a question that is a research problem. The basic unit of the research process is the research project that contains in detail the organization given to this activity and the way it will be carried out. That`s why, it represents a guide for the working team during the development of the research. Every institution reserves itself the right to demand the project format it considers necessary according to its main interests. However, a project should have the following accurate information: title of the research, list of researchers, institution responsible for the research and its management, summary, definition and formulation of the problem, justification of the study, formulation of the hypothesis and objectives, type of study, universe and sample, operationalization of the variables, data collection plan, information processing plan, ethical considerations, resources, bibliographic references, schedule, form of dissemination of the results and annexes
Experiencia cubana con el uso terapéutico de células madre adultas
Hernández-Ramírez,Porfirio; Alfonso-Simón,Amel; Aparicio-Suárez,José L.; Artaza-Sanz,Heriberto; Baganet-Cobas,Aymara; Blanco-Díaz,ángela; Cabrera-Zamora,Maritza; Cruz-Tamayo,Fernando; Díaz-Díaz,Antonio J.; Díaz-Ramírez,Francisco; Dorticós-Balea,Elvira; Fernández-águila,Julio; García-Ibarra,Rafael; Goicoechea-Díaz,Pedro; González-Suárez,Tania; González-Iglesias,Ana I.; Hernández-Ca?ero,Alberto; Lam-Díaz,Rosa M.; León-Amado,Lisset; Macías-Abraham,Consuelo; Macías-González,Raúl; Martínez-de Pinillos,María de los á.; Obregón-Santos,ángel; Peix-González,Amalia; Pérez-Borrego,Amparo; Pol-Marró,Natalia; Socarrás-Ferrer,Berta B.; Suárez-Monteagudo,Carlos; del Valle-Pérez,Lázaro O.; Wilford-de León,Mario;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: las investigaciones básicas y clínicas realizadas en los últimos a?os sobre las células madre y sus posibilidades terapéuticas, son en la actualidad uno de los temas más excitantes de la medicina contemporánea. ya se han obtenido importantes avances en el estudio y aplicación de las células madre adultas que muestran notables ventajas sobre las embrionarias, pues su manipulación resulta más simple, económica y se pueden obtener del propio individuo que va a ser tratado. para la introducción en cuba de la terapia celular regenerativa, en el instituto de hematología e inmunología se seleccionaron como fuentes celulares las células madre adultas derivadas de la médula ósea y las movilizadas a la sangre periférica. para facilitar la extensión del tratamiento a otros centros hospitalarios, se estandarizó una técnica para la movilización de las células madre hematopoyéticas a la sangre periférica, mediante un factor estimulador de colonias de granulocitos (filgrastim, de producción nacional) y se desarrolló un método simple, económico y también más tolerable para los enfermos. de esta forma, se ha extendido la terapia celular a 6 provincias cubanas y hasta abril del a?o 2009 se habían tratado 563 casos con trasplante de células madre adultas autólogas, de los cuales el 81,7 % corresponde a pacientes con enfermedades angiológicas, en los que se ha logrado disminuir significativamente la indicación de amputaciones mayores. también los resultados han sido muy prometedores en las lesiones óseas y procesos periodontales, entre otras enfermedades tratadas. los resultados obtenidos hasta el momento se pueden considerar como un nuevo logro de la ciencia revolucionaria y de nuestros sistemas nacionales de salud y de ciencia y técnica. el método empleado es un proceder económico y factible para instituciones con recursos limitados.
Implante percutáneo de células mononucleares de sangre periférica movilizadas con factor estimulante de colonias granulocíticas, en la osteoartrosis de rodilla. Primer caso comunicado en Cuba
Baganet Cobas,Aymara María; Hernández Ramírez,Porfirio; Fernández Delgado,Norma; Forrellat Barrios,Mariela; González Iglesias,Ana Iris; Pérez Fuentes,Enrique; Macías Abraham,Consuelo; Dorticós Balea,Elvira; Lam Díaz,Rosa M.; Salgado Arocena,Odalys; Villanueva Cagigas,Emilio; León Valladares,Dayneri; Smith García-Menocal,Héctor; Pozo Alonso,Desi; álvarez Hernández,Roberto; Simón Pita,Ana María;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2010,
Abstract: the degenerative joint disease, also known as osteoarthrosis affects to 10% of elderlies aged 60. it is mainly characterized by pain in the involved joint, crepitation, morning stiff and a progressive limitation of movement of that joint leading to a partial or total wear of articular cartilage. the treatment of the knee osteoarthrosis is a great challenge. the recent advances in use of regenerative medicine suggest that adult stem cells could represent a promisor alternative in the treatment of this entity. in a female patient aged 61 presenting with knee osteoarthrosis authors placed a percutaneous implant of autologous mononuclear cells mobilized to peripheral blood by granulocyte colony-stimulating factor achieving a fast clinical and radiological improvement. this result suggests that the procedure used is a feasible, simple, safe and less expensive method for treatment of articular degenerative lesions.
Sexo com animais como prática extrema no porn? bizarro*
Díaz-Benítez, María Elvira;
Cadernos Pagu , 2012, DOI: 10.1590/S0104-83332012000100009
Abstract: within the bizarre porn market, the extreme practice is that of sex with animals. brazil has a worldwide known industry producing this kind of porn. this paper discusses the stigma associated to this kind of production within the porn industry, adds ethnographic data on this market's production, introduces a discussion on the legality, consent and rights and treats the theme from the point of view of eroticism and pleasure, thinking about gender and sexuality in these practices. the question is seen as a vast field to think about dyadic relations like human/animal, normal/abnormal, pleasure/danger, nature/culture.
Valores necesarios en la transformación de la Universidad del Zulia
Rodríguez Domínguez,Karina; Annicchiarico Díaz,Elvira; Díaz-Barrios,Jazmín;
Enlace , 2006,
Abstract: currently organizations require individuals with a commitment capacity, open to knowledge, flexible, that share information as quickly as it change, with a global vision that would allow them opportune decision making. in the face of this situation, we ask ourselves whether luz staff?s organizational values are aligned with the needs of our era. in such a manner, the goal of this descriptive research was to determine the presence of such values among the individuals that make up luz central administration. this articles draws the conclusion that university staff does feel transformation processes as their own due to lack of knowledge, mistrust and little sense of belonging. this obstacles commitment, which could explain successive failures in transformation processes.
Valores necesarios en la transformación de la Universidad del Zulia
Karina Rodríguez Domínguez,Elvira Annicchiarico Díaz,Jazmín Díaz-Barrios
Enlace , 2006,
Abstract: Las organizaciones en la actualidad requieren individuos con capacidad de compromiso, abiertos al aprendizaje,flexibles, que compartan la información tan rápidamente como cambia, con una visión global que le permitala toma de decisiones oportunas. Ante esta situación nos preguntamos si los valores organizacionales del personalde LUZ están alineados a las necesidades de esta era. En ese sentido, el objetivo de esta investigación descriptiva fuedeterminar la presencia de los valores mencionados en los individuos que conforman la Administración Central deLUZ. Se concluye que el personal universitario no siente los procesos de cambio como propios debido a desconocimiento,desconfianza y poco sentido de pertenencia. Lo anterior impide el compromiso, lo cual podría explicar lossucesivos fracasos en el proceso de transformación.
Análisis prospectivo de la ense?anza de prevención de consumo de alcohol y salud reproductiva en facultades/escuelas de educación de Lima-Perú. 2003-2004
Díaz, Elvira Sánchez;Luis, Margarita Antonia Villar;Rassol, Goolan Hussein;Pillon, Sandra Cristina;
Revista Latino-Americana de Enfermagem , 2005, DOI: 10.1590/S0104-11692005000700013
Abstract: this study deals with the teaching of contents related to alcohol use prevention and reproductive health at 4 education schools/colleges in lima-peru. 123 persons participated: teaching authorities, faculty, students and ex-students. the instrument for data collection and prospective analysis was elaborated and validated by the pan-american health organization and adapted to this study. 40 indicators were selected for reproductive health and 32 for alcohol use prevention, which were distributed into 4 basic elements: context, structure, function and comprehensiveness, each of which were submitted to three scenarios, whose situations were defined in a continuum from stability to transformation. in view of these scenario, the results indicate moderate changes to different extents in the indicators (and consequently in the two subject areas), which were present across the 4 elements, in spite of a tendency towards stability that represents the maintenance of traditional perspectives and actions.
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