oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 594 )

2018 ( 819 )

2017 ( 752 )

2016 ( 1106 )

Custom range...

Search Results: 1 - 10 of 462359 matches for " Elke A. Ober "
All listed articles are free for downloading (OA Articles)
Page 1 /462359
Display every page Item
Large-scale mapping of mutations affecting zebrafish development
Robert Geisler, Gerd-J?rg Rauch, Silke Geiger-Rudolph, Andrea Albrecht, Frauke van Bebber, Andrea Berger, Elisabeth Busch-Nentwich, Ralf Dahm, Marcus PS Dekens, Christopher Dooley, Alexandra F Elli, Ines Gehring, Horst Geiger, Maria Geisler, Stefanie Glaser, Scott Holley, Matthias Huber, Andy Kerr, Anette Kirn, Martina Knirsch, Martina Konantz, Axel M Küchler, Florian Maderspacher, Stephan C Neuhauss, Teresa Nicolson, Elke A Ober, Elke Praeg, Russell Ray, Brit Rentzsch, Jens M Rick
BMC Genomics , 2007, DOI: 10.1186/1471-2164-8-11
Abstract: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM.By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes [1,2]. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Genome scanning by bulked segregant analysis with microsatellite markers is the method of choice for such purposes, as a rough map position can be quickly obtained [3,4]. In the zebrafish it is easy to perform mapcrosses against a polymorphic reference line, followed by brother-sister matings among the F1 generation. Linkage to a microsatellite marker can then be found by comparing the band intensities of marker alleles in a pool of mutant F2 individuals with a pool of their wildtype siblings. Because full sibships are analyzed the genetic distance between the mutant locus and a microsatellite can be determine
Short-and long-term health implications of surgical intensive care in the elderly  [PDF]
Stefan Utzolino, Philipp A Ober, Magnus Kaffarnik, Andreas Fischer, Ulrich T Hopt, Peter K Baier
Health (Health) , 2010, DOI: 10.4236/health.2010.212209
Abstract: Background: Treatment of elderly patients on intensive care units is an increasing challenge all over the world. Objectives: To evaluate short- term survival and long-term quality of life im-plications of intensive care for the elderly. Methods: Retrospective analysis of 314 patients 75 years of age or more requiring over 48 hours of intensive care. Results: In multivariate analy-sis, significant risk factors for mortality were chronic renal impairment (OR for survival .30, p < 0.001) and chronic obstructive pulmonary disease (OR 0.48, p = 0.003), pneumonia (OR for non-surviving 3.01, p < 0.001), or thrombosis (OR 1.89, p = 0.003); sepsis was not (OR 1.96, p = 0.055). Therapeutic measures associated with mortality were ventilator therapy > 24 h (OR 4.5), hemodialysis (OR 6.8), and vasopressor therapy (OR 2.5, p < 0.001 for each). A health survey questionnaire in an up to 60-month follow-up of 28 patients revealed considerably lower physi-cal subscores of our patients compared to the general elderly population. Conclusions: Elderly patients benefit from intensive care in terms of survival. Complications are frequent, as are severe consequences for long-term quality of life. Short-term mortality in elderly intensive care patients correlates most closely to pre- existing disease, not age.
Phylogenetic diversification patterns and divergence times in ground beetles (Coleoptera: Carabidae: Harpalinae)
Karen A Ober, Thomas N Heider
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-262
Abstract: According to molecular divergence date estimates, harpalines originated in the mid Cretaceous but did not diversify extensively until the late Cretaceous or early Paleogene about 32 million years after their origin. In a relatively small window of time, harpalines underwent rapid speciation. Harpalines have a relative high net diversification rate and increased cladogenesis in some regions of the clade. We did not see a significant decrease in diversification rate through time in the MCCR test, but a model of diversification with two shift points to lower diversification rates fit the harpaline lineage accumulation through time the best.Our results indicate harpalines are significantly more diverse and have higher diversification than their sistergroup. Instead of an immediate burst of explosive diversification, harpalines may have had a long "fuse" before major lineages diversified during the early Paleogene when other taxa such as mammals, birds, and some flowering plants were also rapidly diversifying.Ever since Darwin, biologists have been interested in using phylogenetic trees to investigate the pattern and tempo of lineage diversification. We see that the branching patterns in phylogenies contain information about the processes of speciation and extinction. Molecular phylogenies of species rich groups supply hypotheses of evolutionary relationships among higher taxa, and also allow us to test hypotheses about the tempo and pattern of diversification [1-4], revealing both the topology of ancestor-descendant relationships and the tempo of descent among members of a clade. It is clear that organismal diversification rates (speciation minus extinction) have been variable across lineages and through time, with some lineages showing slowing rates of lineage accumulation [5] and others showing rapid diversification [6] or radiations. Radiations are generally defined in a way that includes rapid cladogenesis from a common ancestor [7], yielding taxon rich clades. The
Autophagy Induction Is a Tor- and Tp53-Independent Cell Survival Response in a Zebrafish Model of Disrupted Ribosome Biogenesis
Yeliz Boglev,Andrew P. Badrock,Andrew J. Trotter,Qian Du,Elsbeth J. Richardson,Adam C. Parslow,Sebastian J. Markmiller,Nathan E. Hall,Tanya A. de Jong-Curtain,Annie Y. Ng,Heather Verkade,Elke A. Ober,Holly A. Field,Donghun Shin,Chong H. Shin,Katherine M. Hannan,Ross D. Hannan,Richard B. Pearson,Seok-Hyung Kim,Kevin C. Ess,Graham J. Lieschke,Didier Y. R. Stainier,Joan K. Heath
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003279
Abstract: Ribosome biogenesis underpins cell growth and division. Disruptions in ribosome biogenesis and translation initiation are deleterious to development and underlie a spectrum of diseases known collectively as ribosomopathies. Here, we describe a novel zebrafish mutant, titania (ttis450), which harbours a recessive lethal mutation in pwp2h, a gene encoding a protein component of the small subunit processome. The biochemical impacts of this lesion are decreased production of mature 18S rRNA molecules, activation of Tp53, and impaired ribosome biogenesis. In ttis450, the growth of the endodermal organs, eyes, brain, and craniofacial structures is severely arrested and autophagy is up-regulated, allowing intestinal epithelial cells to evade cell death. Inhibiting autophagy in ttis450 larvae markedly reduces their lifespan. Somewhat surprisingly, autophagy induction in ttis450 larvae is independent of the state of the Tor pathway and proceeds unabated in Tp53-mutant larvae. These data demonstrate that autophagy is a survival mechanism invoked in response to ribosomal stress. This response may be of relevance to therapeutic strategies aimed at killing cancer cells by targeting ribosome biogenesis. In certain contexts, these treatments may promote autophagy and contribute to cancer cells evading cell death.
Medical resources presentation on WWW
Anamarija Ro?i?-Hristovski,Sonja Poga?nik,Bo?a Ober
Knji?nica : Revija za Podro?je Bibliotekarstva in Informacijske Znanosti , 1997,
Abstract: Librarians are confronted with new challanges offered by currently developing information technologies. There are more and more information sources in medicine that comprise bibliographic and textual databases, various books and textbooks. These are available on different computer media. The task of librarians is to introduce scientists, health care professionals and students to digital library and present ali available sources, so that they can choose the proper solution for their information needs. CMK has decided to design a home page to present its services and even more medical information resources accessible locally, on the local network and on the Internet.
Treatment of cystoid macular edema with the new-generation NSAID nepafenac 0.1%
Seenu M Hariprasad,Levent Akduman,Joseph A Clever,Michael Ober
Clinical Ophthalmology , 2009,
Abstract: Seenu M Hariprasad1, Levent Akduman2, Joseph A Clever2, Michael Ober3,4, Franco M Recchia5, William F Mieler1,61Department of Ophthalmology and Visual Sciences, Vitreoretinal Service; 6Department of Ophthalmology and Visual Sciences, University of Chicago, Chicago, IL, USA; 2Vitreoretinal Service, Saint Louis University Eye Institute, Saint Louis, MO, USA; 3Vitreoretinal Service, Henry Ford Health Systems, West Bloomfield, MI, USA; 4Retinal Consultants of Michigan, Southfield, MI, USA; 5Vitreoretinal Service, Vanderbilt Eye Institute, Nashville, TN, USAPurpose: To describe the use of nepafenac 0.1% for cystoid macular edema (CME).Methods: This was a multicenter retrospective review of 22 CME cases (20 patients) treated with nepafenac 0.1% (six with concomitant prednisolone acetate 1%) from December 2005 to April 2008: three acute pseudophakic CME cases, 13 chronic/recalcitrant pseudophakic CME cases, and six cases of uveitic CME. Pre- and post-treatment retinal thickness and visual acuity were reported.Results: Following treatment for six weeks to six months, six eyes with uveitic CME showed a mean retinal thickness improvement of 227 ± 168.1 μm; mean best-corrected visual acuity (BCVA) improvement was 0.36 ± 0.20 logMAR. All three cases of acute pseudophakic CME improved after four to 10 weeks of nepafenac, with a mean improvement in retinal thickness of 134 ± 111.0 μm. BCVA improved in two patients (0.16 and 0.22 logMAR) but not in the third due to underlying retinal pigment epithelium changes. Thirteen eyes with chronic/recalcitrant pseudophakic CME demonstrated a mean improvement in retinal thickness of 178 ± 128.7 μm after nepafenac and mean BCVA improvement of 0.33 ± 0.19 logMAR.Conclusion: The positive outcomes of these 22 eyes strongly suggest that nepafenac 0.1% is a promising drug for the treatment of CME. Additional study under randomized controlled conditions is warranted.Keywords: macular edema, NSAID, nepafenac, cataract surgery, uveitis
Tracing planet-induced structures in circumstellar disks using molecular lines
F. Ober,S. Wolf,A. L. Uribe,H. H. Klahr
Physics , 2015, DOI: 10.1051/0004-6361/201526117
Abstract: Circumstellar disks are considered to be the birthplace of planets. Specific structures like spiral arms, gaps, and cavities are characteristic indicators of planet-disk interaction. Investigating these structures can provide insights into the growth of protoplanets and the physical properties of the disk. We investigate the feasibility of using molecular lines to trace planet-induced structures in circumstellar disks. Based on 3D hydrodynamic simulations of planet-disk interactions, we perform self-consistent temperature calculations and produce N-LTE molecular line velocity-channel maps and spectra of these disks using our new N-LTE line radiative transfer code Mol3D. Subsequently, we simulate ALMA observations using the CASA simulator. We consider two nearly face-on inclinations, 5 disk masses, 7 disk radii, and 2 different typical pre-main-sequence host stars (T Tauri, Herbig Ae). We calculate up to 141 individual velocity-channel maps for five molecules/isotopoloques in a total of 32 rotational transitions to investigate the frequency dependence of the structures indicated above. We find that the majority of protoplanetary disks in our parameter space could be detected in the molecular lines considered. However, unlike the continuum case, gap detection is not straightforward in lines. For example, gaps are not seen in symmetric rings but are masked by the pattern caused by the global (Keplerian) velocity field. We identify specific regions in the velocity-channel maps that are characteristic of planet-induced structures. Simulations of high angular resolution molecular line observations demonstrate the potential of ALMA to provide complementary information about the planet-disk interaction as compared to continuum observations. In particular, the detection of planet-induced gaps is possible under certain conditions.(abridged)
Cramer-Rao Lower Bound for Point Based Image Registration with Heteroscedastic Error Model for Application in Single Molecule Microscopy
E. A. K. Cohen,D. Kim,R. J. Ober
Statistics , 2015,
Abstract: The Cramer-Rao lower bound for the estimation of the affine transformation parameters in a multivariate heteroscedastic errors-in-variables model is derived. The model is suitable for feature-based image registration in which both sets of control points are localized with errors whose covariance matrices vary from point to point. With focus given to the registration of fluorescence microscopy images, the Cramer-Rao lower bound for the estimation of a feature's position (e.g. of a single molecule) in a registered image is also derived. In the particular case where all covariance matrices for the localization errors are scalar multiples of a common positive definite matrix (e.g. the identity matrix), as can be assumed in fluorescence microscopy, then simplified expressions for the Cramer-Rao lower bound are given. Under certain simplifying assumptions these expressions are shown to match asymptotic distributions for a previously presented set of estimators. Theoretical results are verified with simulations and experimental data.
Componentes normativas de alta incidencia en la nueva morfología del Santiago Metropolitano: una revisión crítica de la norma de "Conjunto Armónico"
Schlack,Elke; Vicu?a,Magdalena;
EURE (Santiago) , 2011, DOI: 10.4067/S0250-71612011000200006
Abstract: increased height, higher residential density and typological differentiation regarding the environment are the main characteristics of buildings that are currently constructed int eh context of the chilean legalnorm known as "harmonic set". considering that this norm promotes the morphological and functional exception of such projects regarding their environment, it is maintained that this regulation could represent one way to regulate certain exceptional buildings in the city: those that today are known as mega-projects. this articule illustrates two moments in the evoluation of this legal norm. in both, the intent to form relevant urban archetypes can be clearly seen. it shows the evolution of this norm and exemplifies this process through cases from the initial period, associated with the compositional vision (1930-50), and a second period associated with the functionalist vision of the city (1960-80). the objectives associated with each period are compared and contrasted with the qualities that currently promote the legal norm of harmonic set. we reflect upon the pertienence of the norm, and discuss the possibilities that this instrument provides today for the creation of urban archetypes.
Componentes normativas de alta incidencia en la nueva morfología del Santiago Metropolitano: una revisión crítica de la norma de "Conjunto Armónico"
Elke Schlack,Magdalena Vicu?a
EURE (Santiago) , 2011,
Abstract: Mayor altura, mayor densidad habitacional y diferenciación tipológica respecto del entorno son las principales cualidades de los edificios que se construyen actualmente en el marco de la normativa chilena llamada "Conjunto Armónico". Considerando que esta normativa promueve la excepción morfológica y funcional de los proyectos respecto a su entorno, se sostiene que esta regulación podría constituir una manera de normar edificios excepcionales en la ciudad: aquello que hoy se conoce como "megaproyectos". Este artículo ilustra dos momentos en la evolución de esta norma. En ambos se puede visualizar la intención de constituir piezas de relevancia urbana. Se muestra la evolución de la norma y se ejemplifica a través de casos del periodo inicial, asociado a la visión composicional (1930-50), y de un segundo período, asociado a la visión funcionalista de la ciudad (1960-80). Se contrastan los objetivos asociados a estas dos etapas, con las cualidades que actualmente promueve la norma de Conjunto Armónico. Se reflexiona acerca de la pertinencia actual de la norma y se comentan las posibilidades que brinda hoy este instrumento para la gestión de piezas de relevancia urbana. Increased height, higher residential density and typological differentiation regarding the environment are the main characteristics of buildings that are currently constructed int eh context of the Chilean legalnorm known as "Harmonic Set". Considering that this norm promotes the morphological and functional exception of such projects regarding their environment, it is maintained that this regulation could represent one way to regulate certain exceptional buildings in the city: those that today are known as mega-projects. This articule illustrates two moments in the evoluation of this legal norm. In both, the intent to form relevant urban archetypes can be clearly seen. It shows the evolution of this norm and exemplifies this process through cases from the initial period, associated with the compositional vision (1930-50), and a second period associated with the functionalist vision of the city (1960-80). The objectives associated with each period are compared and contrasted with the qualities that currently promote the legal norm of harmonic set. We reflect upon the pertienence of the norm, and discuss the possibilities that this instrument provides today for the creation of urban archetypes.
Page 1 /462359
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.