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Search Results: 1 - 10 of 404160 matches for " Elenice M Sequetin; Lara "
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Isolamento do vírus da raiva em Artibeus fimbriatus no Estado de S?o Paulo
Cunha,Elenice M Sequetin; Lara,Maria do Carmo C S H; Nassar,Alessandra Figueiredo de Castro; Sodré,Miriam M; Amaral,Luis Flávio Vani;
Revista de Saúde Pública , 2005, DOI: 10.1590/S0034-89102005000400025
Abstract: this is the first report of the isolation and identification of the rabies virus in the frugivorous bat artibeus fimbriatus in the city of sao josé do rio preto, sao paulo state, brazil. the virus was isolated from an animal found in an urban area. the animal was found on the ground under a tree, still alive. diagnosis was made by direct immunofluorescence and intracerebral inoculation of mice.
Seroprevalence of equine viral arteritis in paulista mesoregions between 2007 and 2008 Soroprevalência da arterite viral equina em mesorregi es paulistas entre 2007 e 2008
Pollyana Rennó Campos Braga,Maria do Carmo Custódio de Souza Hunold Lara,Adriano Dias,Elenice Maria Sequetin Cunha
Semina : Ciências Agrárias , 2012,
Abstract: O presente estudo investigou a ocorrência de anticorpos contra o vírus da arterite equina, utilizando a técnica de soroneutraliza o em 1.400 equinos criados nas mesorregi es macro metropolitana paulista e Campinas do estado de S o Paulo (SP), entre os anos de 2007 e 2008. Do total das amostras, 80 (5,7%) apresentaram anticorpos para o vírus (títulos entre 4 e 4096). Dentre os 42 municípios amostrados, 15 (35,7%) apresentaram pelo menos um animal sororeagente. Foram analisadas 238 propriedades das quais 41 apresentaram ao menos um animal sororeagente. A ocorrência de animais reagentes foi maior em cavalos que eram utilizados para práticas esportivas, particularmente das ra as utilizadas para salto e Quarto de Milha e foi semelhante entre machos e fêmeas. A soropositividade também foi maior em animais com idade superior a 24 meses. Os resultados obtidos sugerem a circula o do vírus da arterite equina nos criatórios amostrados e refor am a necessidade de estudos continuados de vigilancia epidemiológica. O presente estudo investigou a ocorrência de anticorpos contra o vírus da arterite equina, utilizando a técnica de soroneutraliza o em 1.400 equinos criados nas mesorregi es macro metropolitana paulista e Campinas do estado de S o Paulo (SP), entre os anos de 2007 e 2008. Do total das amostras, 80 (5,7%) apresentaram anticorpos para o vírus (títulos entre 4 e 4096). Dentre os 42 municípios amostrados, 15 (35,7%) apresentaram pelo menos um animal sororeagente. Foram analisadas 238 propriedades das quais 41 apresentaram ao menos um animal sororeagente. A ocorrência de animais reagentes foi maior em cavalos que eram utilizados para práticas esportivas, particularmente das ra as utilizadas para salto e Quarto de Milha e foi semelhante entre machos e fêmeas. A soropositividade também foi maior em animais com idade superior a 24 meses. Os resultados obtidos sugerem a circula o do vírus da arterite equina nos criatórios amostrados e refor am a necessidade de estudos continuados de vigilancia epidemiológica.
Isolamento do vírus rábico em Molossus ater (Chiroptera: Molossidae) no Estado de S o Paulo
Silva Luzia H Queiroz da,Cunha Elenice M Sequetin,Pedro Wagner André,Cardoso Teresa C
Revista de Saúde Pública , 1999,
Abstract: Descreve-se o isolamento e a identifica o do vírus rábico em morcegos insetívoros Molossus ater, no Estado de S o Paulo, nos municípios de Ara atuba, Penápolis e S o José do Rio Preto. A maioria dos exemplares foi capturada ainda com vida, n o havendo, porém, contato com pessoas ou animais. O diagnóstico foi realizado pelas provas de imunofluorescência direta e inocula o intracerebral em camundongos.
Detection of anti-Toxoplasma gondii antibodies in carthorses in the metropolitan region of Curitiba, Paraná, Brazil Detec o de anticorpos anti-Toxoplasma gondii em cavalos carroceiros da regi o metropolitana de Curitiba, Paraná, Brasil
Mariane Angélica Finger,Eliana Monteforte Cassaro Villalobos,Maria do Carmo Custódio de Souza Hunold Lara,Elenice Maria Sequetin Cunha
Revista Brasileira de Parasitologia Veterinária , 2013,
Abstract: Toxoplasma gondii, the agent for toxoplasmosis, has worldwide distribution. Horses normally play a secondary role in its life cycle, but movement around urban areas, feeding on grass and the increasing use of carthorses for gathering recyclable material in some urban areas of Brazil may increase their exposure to T. gondii infection. The aim of the present study was to investigate the frequency of anti-T. gondii antibodies in carthorses in the metropolitan region of Curitiba, PR. IgG antibodies against T. gondii were detected using the indirect fluorescence antibody test (IFAT) (titers ≥ 64). Seventeen (17.0%) of the 100 horses sampled were seropositive. There were no statistical differences in relation to sex (p = 0.28) or age (p = 0.15). Our findings suggest that carthorses are exposed to T. gondii infections and that no associations with age or sex exist. Toxoplasma gondii, agente da toxoplasmose, tem distribui o mundial. Geralmente cavalos desempenham papel secundário no ciclo de vida do T. gondii. Entretanto, a circula o em meio urbano, a alimenta o com gramíneas e o aumento no uso de cavalos carroceiros para recolhimento de material reciclável em algumas áreas urbanas do Brasil podem aumentar a exposi o desses cavalos ao T. gondii. O objetivo deste estudo foi investigar a frequência de anticorpos anti-T. gondii em cavalos carroceiros da regi o metropolitana de Curitiba, PR. Os anticorpos da classe IgG contra T. gondii foram detectados pela rea o de imunofluorescência indireta (RIFI) (títulos ≥ 64). Dezessete (17%) dos 100 cavalos avaliados foram positivos e n o houve diferen a entre sexos (p = 0,28) ou idade (p = 0,15). Esses achados sugerem que cavalos carroceiros est o expostos a infec es por T. gondii, n o existindo associa o com a idade ou o sexo dos animais.
Rabies laboratory diagnosis: peculiar features of samples from equine origin
Peixoto Zélia M.P.,Cunha Elenice M. Sequetin,Sacramento Débora R.V.,Souza M. Concei??o A.M.
Brazilian Journal of Microbiology , 2000,
Abstract: Rabies laboratory diagnosis is performed by using microscopic examination for Negri bodies (MEN), fluorescent-antibody test (FAT) and mouse inoculation test (MIT). In the majority of cases, when specimens are properly collected and conserved and the laboratory worker has good experience, agreement among employed techniques is verified. Comparing the sensitivity of these three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from bovine (983 positives) and 703 from equine (111 positives) species, it was observed that in equine rabid samples, this agreement was not maintained. For the latter specie, only in few opportunities the Negri bodies could be observed. With respect to FAT, the test detected a lower porcentage of positive equine samples compared to bovine species. Statistical analysis demonstrated that the difference was significative. Mouse inoculation test proved to be more sensitive. However, a significant difference in mice incubation period was observed for samples from both species. The absence of inclusion bodies and the longer incubation period for equine samples suggest that rabies pathogenesis studies for equine species have to be intensified.
Isolamento do vírus rábico em Molossus ater (Chiroptera: Molossidae) no Estado de S?o Paulo
Silva,Luzia H Queiroz da; Cunha,Elenice M Sequetin; Pedro,Wagner André; Cardoso,Teresa C; Souza,Maria do Carmo C de; Ferrari,Clara Isabel L;
Revista de Saúde Pública , 1999, DOI: 10.1590/S0034-89101999000600016
Abstract: this is a report of rabies infection in insectivorous bat molossus ater in the city districts of ara?atuba, penápolis and s?o josé do rio preto, in s?o paulo state, brazil. fluorescent antibody test detected the virus in the brain and isolation was obtained by intracerebral inoculation of mice with nervous tissue and organs suspension. there was no contact with humans or other animals.
Bat rabies in the north-northwestern regions of the state of S?o Paulo, Brazil: 1997-2002
Cunha,Elenice Maria Sequetin; Silva,Luzia Helena Queiroz da; Lara,Maria do Carmo Custódio Souza Hunold; Nassar,Alessandra Figueiredo Castro; Albas,Avelino; Sodré,Mirian Matos; Pedro,Wagner André;
Revista de Saúde Pública , 2006, DOI: 10.1590/S0034-89102006000700017
Abstract: objective: reports on bat rabies in brazil are sporadic and isolated. this study aimed at describing the detection of rabies virus in bats in the state of s?o paulo. methods: a total of 7,393 bats from 235 municipalities of the north and northwestern areas of the state of s?o paulo, southeastern brazil, were assessed according to their morphological and morphometric characteristics from 1997 to 2002. fluorescent antibody test and mice inoculation were used for viral identification. results: of all samples examined, 1.3% was rabies virus positive, ranging from 0.2% in 1997 to 1.6% in 2001. there were found 98 bats infected, 87 in the urban area. fluorescent antibody test was detected in 77 positive samples, whereas 92 produced rabies signs in mice; incubation period ranging from 4 to 23 days. in 43 cities at least one rabid bat was observed. the highest proportion (33.7%) of rabies virus was found in artibeus lituratus. eptesicus and myotis were the most frequent positive species (24.5%) of the vespertilionidae family. the species molossus molossus and molossus rufus showed 14.3% positive bats. there were no differences in the distribution of positive rabies between females (33; 48.5%) and males (35; 51.5%). conclusions: rabies-infected bats were found in environments that pose a risk to both human and domestic animal population and there is a need for actions aiming at the control of these species and public education.
Rabies laboratory diagnosis: peculiar features of samples from equine origin
Peixoto, Zélia M.P.;Cunha, Elenice M. Sequetin;Sacramento, Débora R.V.;Souza, M. Concei??o A.M.;Silva, Luzia H. Queiroz da;Germano, Pedro Leal;Kroeff, Suzana S.;Kotait, Ivanete;
Brazilian Journal of Microbiology , 2000, DOI: 10.1590/S1517-83822000000100017
Abstract: rabies laboratory diagnosis is performed by using microscopic examination for negri bodies (men), fluorescent-antibody test (fat) and mouse inoculation test (mit). in the majority of cases, when specimens are properly collected and conserved and the laboratory worker has good experience, agreement among employed techniques is verified. comparing the sensitivity of these three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from bovine (983 positives) and 703 from equine (111 positives) species, it was observed that in equine rabid samples, this agreement was not maintained. for the latter specie, only in few opportunities the negri bodies could be observed. with respect to fat, the test detected a lower porcentage of positive equine samples compared to bovine species. statistical analysis demonstrated that the difference was significative. mouse inoculation test proved to be more sensitive. however, a significant difference in mice incubation period was observed for samples from both species. the absence of inclusion bodies and the longer incubation period for equine samples suggest that rabies pathogenesis studies for equine species have to be intensified.
Detection of Neospora sp. antibodies in cart horses from urban areas of Curitiba, Southern Brazil
Villalobos, Eliana Monteforte Cassaro;Furman, Keiko Endo;Lara, Maria do Carmo Custódio de Souza Hunold;Cunha, Elenice Maria Sequetin;Finger, Mariane Angélica;Busch, Ana Paula Brenner;Barros Filho, Ivan Roque de;Deconto, Ivan;Dornbusch, Peterson Triches;Biondo, Alexander Welker;
Revista Brasileira de Parasitologia Veterinária , 2012, DOI: 10.1590/S1984-29612012000100014
Abstract: neospora caninum is a protozoan parasite which affects dogs as definitive hosts and several mammalian species as intermediate hosts mainly causing abortions and central nervous system disorders. the reemerging population of cart horses for carrying recycling material in urban areas of major cities in brazil may have an impact on disease spreading, and these animals may be used as sentinels for environmental surveillance. thus, the present study investigated the frequency of neospora sp. antibodies in cart horses from curitiba and surrounding areas, paraná state, southern brazil. igg antibodies against neospora sp. were detected using indirect fluorescence antibody test (ifat), and titers equal to or higher than 1:50 were considered reactive. of all samples, 14/97 (14.4%) were positive: 2/29 (6.9%) were younger than 5; 5/26 (19.2%) between 6 and 9; and 6/31 (19.4%) older than 10 years of age. one of the 11 animals with unknown age was positive (9.1%). cart horses are likely to be more exposed to dog feces and to neospora sp. oocyst contamination in urban settings and a lower frequency of disease in dogs may have a negative impact on horse infection risk in these areas.
Diagnóstico laboratorial da raiva na regi?o oeste do Estado de S?o Paulo
Albas, Avelino;Zoccolaro, Paulo Tadeu;Rosa, Talita Zacarias;Cunha, Elenice Maria Sequetin;
Revista da Sociedade Brasileira de Medicina Tropical , 2005, DOI: 10.1590/S0037-86822005000600009
Abstract: the pólo da alta sorocabana laboratory, presidente prudente, sp, brazil, and the biological institute in s?o paulo state, performed an evaluation of rabies diagnosis from 1996 to 2003 in the west region of s?o paulo state. for the tests, the laboratories used direct immunofluorescence and mice inoculation in 4,950 samples, that were sent for analysis involving dogs, cats, cattle, chiroptera (bats) and other animals. according to the results, the laboratories found 74 positive samples; of which 58 (78.4%)were non-hematophagous bats and 16 (21.6%) related to cattle. the present epidemiological study verified that in spite of the high positive index in chiroptera compared to the other species, there was not an outbreak of rabies in the species in the region of presidente prudente, from 1996 to 2003 but a rise in the positive index due to a marked increase in the number of chiroptera samples sent to the laboratories for virus rabies research.
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