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Search Results: 1 - 10 of 2409 matches for " Elaine Pittner "
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Aflatoxinas: um risco a saúde humana e animal / Aflatoxins: a risk animal and human health
Helder Ferreira,Elaine Pittner,Hermes Francisco Sanches,Marta Chagas Monteiro
Ambiência , 2006,
Abstract: O Aspergillus flavus e a subespécie próxima relacionada parasiticus têm sido reconhecidas por muito tempo como contaminadores principais de produtos organicos e inorganicos. A. flavus, um fungo comum do solo, pode infestar uma larga escala de produtos agrícolas. Algumas variedades de A. flavus produzem as aflatoxinas, que s o toxinas neoplásicas capazes de induzir neoplasia hepática em animais de laboratório. O crescimento de A. flavus e a biossíntese da aflatoxina depende da carca a, da umidade, da temperatura, do pH, da aera o e da microflora competindo. As aflatoxinas s o consideradas contaminantes naturais; cuja aproxima o ideal de controle é a preven o do crescimento do fungo e produ o das aflatoxinas nos produtos. As aflatoxinas (B1, B2, G1 e G2) s o metabólitos secundários associados à toxicidade causada por alimenta es em animais. As aflatoxinas s o relatadas como sendo hepatotóxicas, mutagênicas, imunossupressora e neoplásicas. A exposi o as aflatoxinas dietéticas é considerada um fator de risco importante para o desenvolvimento de neoplasia hepatocelular em determinadas regi es do mundo. A indu o de adutos no DNA pela aflatoxina B1 no fígado foi revista extensivamente em uma avalia o quantitativa de fatores de risco a neoplasias por aflatoxinas. As aflatoxinas s o pró-neoplásicas, reagindo com o ácido desoxirribonucléico (DNA), ácido ribonucléico (RNA) e as proteínas. Atualmente, vários avan os científicos têm sido feitos a fim de compreender a toxicologia clínica das aflatoxinas.
Detec??o de casos novos de hanseníase no município de Prudentópolis, PR: uma análise de 1998 a 2005
Sanches, Lucas Augusto Thomé;Pittner, Elaine;Sanches, Hermes Francisco;Monteiro, Marta Chagas;
Revista da Sociedade Brasileira de Medicina Tropical , 2007, DOI: 10.1590/S0037-86822007000500010
Abstract: the aim of this study was to evaluate the detection of new cases of leprosy in prudentópolis, state of paraná, from 1998 to 2005. additionally, we investigated the age group and predominant clinical form of leprosy cases. data were obtained from clinical-epidemiological records of confirmed leprosy cases that were notified in the information system for notifiable diseases, between 1998 and 2005. over this period, 222 leprosy cases were notified, of which 63% presented the multibacillary form, with predominance of the virchow clinical form, thus indicating a process of intense disease transmission. among these leprosy cases, 35% of the patients were at an economically active age (31 to 45 years old). these indicators showed that there was a high level of bacillus circulation in the community, considering that one risk factor for infection by the bacillus mycobacterium leprae is contact with untreated individuals who have the multibacillary form. this is despite the drastic reduction in leprosy prevalence over the last two decades, through the implementation of polychemotherapy and other preventive measures.
Bericht & Report
Pittner H
Journal für Ern?hrungsmedizin , 2007,
Abstract:
Spatial distribution of enteroparasites among school children from Guarapuava, State of Paraná, Brazil
Buschini, Maria Luisa Tunes;Pittner, Elaine;Czervinski, Tiago;Moraes, Ivonete de Fátima;Moreira, M?nica Monteiro;Sanches, Hermes Francisco;Monteiro, Marta Chagas;
Revista Brasileira de Epidemiologia , 2007, DOI: 10.1590/S1415-790X2007000400015
Abstract: the most common infections in human beings are caused by intestinal parasites. they can lead to a number of harmful effects, which could include, among others, intestinal obstruction, malnutrition, iron deficiency anemia, diarrhea, and poor absorption. in brazil, enteroparasites are one of the main public health issues. the present study aims at analyzing the distribution and frequency of enteroparasite occurrence in 635 children from seven community schools in the city of guarapuava, paraná (pr). in addition, we used similarity and diversity indices to analyze the parasite community. we found 475 samples with at least one parasite showing that 75.27% of children had enteroparasites. a smaller fraction (26.73%) of children harbored several parasites (multiparasitism), especially giardia duodenalis (56%), and ascaris lumbricoides (18%). statistical analysis showed that three (out of seven) children communities had higher similarity in frequency and amount of parasites. our results suggest that the children studied were highly infected by enteroparasites. these levels of infestation could be related to several factors, such as climate, social and economic conditions and characteristics of the parasites.
Quantum computing applied to calculations of molecular energies: CH2 benchmark
Libor Veis,Ji?í Pittner
Physics , 2010, DOI: 10.1063/1.3503767
Abstract: Quantum computers are appealing for their ability to solve some tasks much faster than their classical counterparts. It was shown in [Aspuru-Guzik et al., Science 309, 1704 (2005)] that they, if available, would be able to perform the full configuration interaction (FCI) energy calculations with a polynomial scaling. This is in contrast to conventional computers where FCI scales exponentially. We have developed a code for simulation of quantum computers and implemented our version of the quantum full configuration interaction algorithm. We provide a detailed description of this algorithm and the results of the assessment of its performance on the four lowest lying electronic states of CH2 molecule. This molecule was chosen as a benchmark, since its two lowest lying 1A1 states exhibit a multireference character at the equilibrium geometry. It has been shown that with a suitably chosen initial state of the quantum register, one is able to achieve the probability amplification regime of the iterative phase estimation algorithm even in this case.
Biocompatible medical implant materials with binding sites for a biodegradable drug-delivery system
Al-Dubai H,Pittner G,Pittner F,Gabor F
Nanotechnology, Science and Applications , 2011,
Abstract: Haifa Al-Dubai1, Gisela Pittner1, Fritz Pittner1, Franz Gabor21Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria; 2Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, Vienna, AustriaAbstract: Feasibility studies have been carried out for development of a biocompatible coating of medical implant materials allowing the binding of biodegradable drug-delivery systems in a way that their reloading might be possible. These novel coatings, able to bind biodegradable nanoparticles, may serve in the long run as drug carriers to mediate local pharmacological activity. After biodegradation of the nanoparticles, the binding sites could be reloaded with fresh drug-delivering particles. As a suitable receptor system for the nanoparticles, antibodies are anchored. The design of the receptor is of great importance as any bio- or chemorecognitive interaction with other components circulating in the blood has to be avoided. Furthermore, the binding between receptor and the particles has to be strong enough to keep them tightly bound during their lifetime, but on the other hand allow reloading after final degradation of the particles. The nanoparticles suggested as a drug-delivery system for medical implants can be loaded with different pharmaceuticals such as antibiotics, growth factors, or immunosuppressives. This concept may enable the changing of medication, even after implantation of the medical device, if afforded by patients’ needs.Keywords: antibody immobilization, biocompatible coating, chitosan nanoparticles, drug targeting, medical device
Four-Reference State-Specific Brillouin-Wigner Coupled-Cluster Method: Study of the IBr Molecule
Ji?í Pittner,Ond?ej Demel,Petr ?ársky,Ivan Huba?
International Journal of Molecular Sciences , 2001, DOI: 10.3390/i2060281
Abstract: We implemented the state-specific Brillouin–Wigner coupled-cluster method for the complete model space spanned by four reference configurations generated by two electrons in two active orbitals. We applied the method (together with the previously suggested a posteriori size-extensivity correction) to the calculation of spectroscopic constants of the IBr molecule, using averaged relativistic effective core potential.
Orienting a new generation of nurses: Expectations of the millennial new graduate  [PDF]
Elaine M. Riegel
Open Journal of Nursing (OJN) , 2013, DOI: 10.4236/ojn.2013.37062
Abstract:

Generational diversity provides a unique quandary for nursing leaders and educators to enlist, orient, and retain nurses. Millennials are the largest cohort since the Baby Boomers and the age group from which the nursing profession will build its future workforce. This study examined the experiences of the new graduate nurse of the millennial generation as they navigate orientation in an acute care setting. Using a Web-based survey to conduct the study allowed nurses from across the country to participate while providing easy access to the study questions. Analysis of the data through qualitative content analysis demonstrated a dominate theme of seeking structure while expecting an individualized orientation. The survey also revealed the importance of the preceptor and manager in the perceived success of this generation’s transition and a need to assimilate into the professional role of RN. Recognizing that there is a need to promote a more individualized orientation with improved mentoring for novice nurses and their preceptors is evident and challenges nursing leaders and educators to rethink current practices to retain new nurses. Established roles within the hospital and individual nursing units are changing and traditional strategies for recruitment and retention no longer apply. A structured orientation can facilitate the transition from new graduate to professional nurse and assist in the retention of qualified nurses and ultimately safe patient care.

Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo
Wolfgang Benetka, Manfred Koranda, Sebastian Maurer-Stroh, Fritz Pittner, Frank Eisenhaber
BMC Biochemistry , 2006, DOI: 10.1186/1471-2091-7-6
Abstract: We describe a new technique for detecting prenyl anchors in N-terminally glutathione S-transferase (GST)-labeled constructs of target proteins expressed in vitro in rabbit reticulocyte lysate and incubated with 3H-labeled anchor precursors. Alternatively, hemagglutinin (HA)-labeled constructs expressed in vivo (in cell culture) can be used. For registration of the radioactive marker, we propose to use a thin layer chromatography (TLC) analyzer. As a control, the protein yield is tested by Western blotting with anti-GST- (or anti-HA-) antibodies on the same membrane that has been previously used for TLC-scanning. These protocols have been tested with Rap2A, v-Ki-Ras2 and RhoA (variant RhoA63L) including the necessary controls. We show directly that RasD2 is a farnesylation target.Savings in time for experimentation and the higher sensitivity for detecting 3H-labeled lipid anchors recommend the TLC-scanning method with purified GST- (or HA-) tagged target proteins as the method of choice for analyzing their prenylation capabilities in vitro and in vivo and, possibly, also for studying the myristoyl and palmitoyl posttranslational modifications.Prenylation is a lipid posttranslational modification (PTM) of proteins at cysteine residues in the C-terminal region [1-7]. The specific sequence environment recognized by prenyltransferases consists either of the CaaX box for farnesyltransferase (FTase) and geranylgeranyltransferase 1 (GGTase1) or C-terminal cysteines of Rab GTPases in the case of geranylgeranyltransferase 2 (GGTase2). In all instances, the cysteine-containing region must be preceded on the N-terminal side by approximately 10 residues providing a generally polar, flexible, so called linker segment without inherent conformational preferences [7]. The anchor can be of farnesyl (3 isoprenyl units) or of geranylgeranyl (4 isoprenyl units) type [8]. Targeting to cellular membranes [1,9] and mediation of protein-protein interactions [10-16] are well documented biolo
Adaptation and Validation of a Proteolytic Activity Methodology Using Dye-Crosslinked Substrate Tablets  [PDF]
Fernanda Martins, Elaine C. Ramires
Advances in Enzyme Research (AER) , 2014, DOI: 10.4236/aer.2014.23012
Abstract: Enzymatic activities are important to be quantified in products as enzymatic cleaners, which are used in medical and surgical devices reprocessing. Enzymatic activities are critical for the proper chemical cleaning that intends to remove solid organic dirt from inaccessible sites. The most important enzyme for this purpose is the protease, which is able to dissolve the main dirt attached to medical and surgical instruments. In this context, this study contributes to the development of a new proteolytic activity quantification method and its validation. The methodology is based on colorimetry and uses a UV-Vis spectrophotometer to measure the substrate hydrolysis by the blue color intensity, employing Protazyme AK tablets as substrate.
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