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Search Results: 1 - 10 of 403512 matches for " Elaine M Forbes "
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Control of gag-pol gene expression in the Candida albicans retrotransposon Tca2
Elaine M Forbes, Sian R Nieduszynska, Fiona K Brunton, Joanne Gibson, L Anne Glover, Ian Stansfield
BMC Molecular Biology , 2007, DOI: 10.1186/1471-2199-8-94
Abstract: The hypothesis was tested that in Tca2, gag stop codon flanking sequences direct translational readthrough and synthesis of a gag-pol fusion protein. Sequence from the Tca2 gag-UGA-pol junction (300 nt) was inserted between fused lacZ and luciferase (luc) genes in a Saccharomyces cerevisiae dual reporter construct. Although downstream of UGA, luc was expressed, but its expression was unaffected by inserting additional stop codons at the 3' end of lacZ. Luc expression was instead being driven by a previously unknown minor promoter activity within the gag-pol junction region. Evidence together indicated that junction sequence alone cannot direct UGA readthrough. Using reporter genes in C. albicans, the activities of this gag-pol junction promoter and the Tca2 long terminal repeat (LTR) promoter were compared. Of the two promoters, only the LTR promoter was induced by heat-shock, which also triggers retrotransposition. Tca2 pol protein, epitope-tagged in C. albicans to allow detection, was also heat-shock induced, indicating that pol proteins were expressed from a gag-UGA-pol RNA.This is the first demonstration that the LTR promoter directs Tca2 pol protein expression, and that pol proteins are translated from a gag-pol RNA, which thus requires a mechanism for stop codon bypass. However, in contrast to most other retroelement and viral readthrough signals, immediate gag UGA-flanking sequences were insufficient to direct stop readthrough in S. cerevisiae, indicating non-canonical mechanisms direct gag UGA bypass in Tca2.Retrotransposons are mobile genetic elements that replicate via an RNA intermediate. Their replication cycles and genome organisation are similar in many respects to those of retroviruses, and retrotransposons form a cytoplasmic virus-like particle during replication. Both groups of retroelements have a genome comprising gag and pol coding sequences. Gag proteins make up the capsid of the viral particle while pol encodes a polyprotein usually made up of
Orienting a new generation of nurses: Expectations of the millennial new graduate  [PDF]
Elaine M. Riegel
Open Journal of Nursing (OJN) , 2013, DOI: 10.4236/ojn.2013.37062
Abstract:

Generational diversity provides a unique quandary for nursing leaders and educators to enlist, orient, and retain nurses. Millennials are the largest cohort since the Baby Boomers and the age group from which the nursing profession will build its future workforce. This study examined the experiences of the new graduate nurse of the millennial generation as they navigate orientation in an acute care setting. Using a Web-based survey to conduct the study allowed nurses from across the country to participate while providing easy access to the study questions. Analysis of the data through qualitative content analysis demonstrated a dominate theme of seeking structure while expecting an individualized orientation. The survey also revealed the importance of the preceptor and manager in the perceived success of this generation’s transition and a need to assimilate into the professional role of RN. Recognizing that there is a need to promote a more individualized orientation with improved mentoring for novice nurses and their preceptors is evident and challenges nursing leaders and educators to rethink current practices to retain new nurses. Established roles within the hospital and individual nursing units are changing and traditional strategies for recruitment and retention no longer apply. A structured orientation can facilitate the transition from new graduate to professional nurse and assist in the retention of qualified nurses and ultimately safe patient care.

Present Trends in Systematic Entomology.Scientific Names
Wm. T. M. Forbes
Psyche , 1929, DOI: 10.1155/1929/75062
Abstract:
The Family Position of Graphelysia (Lepidoptera)
Wm. T. M. Forbes
Psyche , 1924, DOI: 10.1155/1924/27940
Abstract:
Two Wasp-Guests From Puerto Rico (Microlepidoptera)
Wm. T. M. Forbes
Psyche , 1933, DOI: 10.1155/1933/56460
Abstract:
How a Beetle Folds its Wings
Wm. T. M. Forbes
Psyche , 1924, DOI: 10.1155/1924/68247
Abstract:
On the Tymipanum of Certain Lepidoptera
William T. M. Forbes
Psyche , 1916, DOI: 10.1155/1916/16801
Abstract:
The Persius Group of Thanaos (Lepidoptera, Hesperiidae)
William T. M. Forbes
Psyche , 1936, DOI: 10.1155/1936/81439
Abstract:
The Lepidoptera of the Dry Tortugas
Wm. T. M. Forbes
Psyche , 1941, DOI: 10.1155/1941/87969
Abstract:
The Protocoleoptera
Wm. T. M. Forbes
Psyche , 1928, DOI: 10.1155/1928/56970
Abstract:
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