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Search Results: 1 - 10 of 259765 matches for " Eduardo José de Almeida; Sant'Ana "
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Intestinal Ascending Colon Morphometrics in Rats Submitted to Severe Protein Malnutrition
Hermes,Catchia; Azevedo,Jorge Fernandes de; Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gon?ales;
International Journal of Morphology , 2008, DOI: 10.4067/S0717-95022008000100001
Abstract: this paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). in the following 90 days, the rats of the control group received a 24% protein chow as the malnourished group received 4% protein chow. the animals were submitted to euthanasia according to the anesthetic protocol. colon segments were collected and submitted to routine histological processing. the cuts were stained with he and histochemical techniques for mucines. the morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of goblet cells, the depth of the crypt and the height of the enterocytes as well as their nucleus on malnutritioned animals. the data suggest that protein malnutrition causes alterations on adult rat ascending colon intestinal morphometrics, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells, and crypts.
Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
Franco,Cássia Luiza de Marins; Sant'Ana,Débora de Mello Gon?ales; Araújo,Eduardo José de Almeida;
International Journal of Morphology , 2010, DOI: 10.4067/S0717-95022010000200026
Abstract: the effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. six 90-day-old wistar rats (rattus norvegicus) were assigned into two groups: cg (control group, n = 3) and mg (malnourished group, n = 3). cg animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. after 90 days, part of the jejunum was collected and subjected to routine histology. he-staining and histochemical techniques - periodic acid schiff (pas) and alcian blue (ab), ph 2.5/ph 1.0 - were used for the detection of glycoconjugates in 4μm -thick transverse cuts. morphometric analysis of the he-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to pas and ab ph 2.5, whereas the number of cells reactive to ab ph 1.0 increased. protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium.
Morfometría de la Pared Intestinal del Colon Ascendente de Ratas Sometidas a Desnutrición Proteica Severa Intestinal Ascending Colon Morphometrics in Rats Submitted to Severe Protein Malnutrition
Catchia Hermes,Jorge Fernandes de Azevedo,Eduardo José de Almeida Araújo,Débora de Mello Gon?ales Sant'Ana
International Journal of Morphology , 2008,
Abstract: This paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). In the following 90 days, the rats of the control group received a 24% protein chow as the malnourished group received 4% protein chow. The animals were submitted to euthanasia according to the anesthetic protocol. Colon segments were collected and submitted to routine histological processing. The cuts were stained with HE and histochemical techniques for mucines. The morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of goblet cells, the depth of the crypt and the height of the enterocytes as well as their nucleus on malnutritioned animals. The data suggest that protein malnutrition causes alterations on adult rat ascending colon intestinal morphometrics, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells, and crypts. El objetivo de este trabajo fue verificar los efectos de la desnutrición proteica severa sobre la morfometría de la pared intestinal del colon ascendente de ratas adultas. Fueron utilizadas 12 ratas (90 días de edad), divididas en dos grupos: control (n=5) y desnutrido (n=7). En los 90 días siguientes, las ratas del grupo control recibieron ración con 24% de contenido proteico y los del grupo desnutrido con 4%. Los animales fueron eutanasiados de acuerdo al protocolo anestésico. Segmentos del colon fueron retirados y sometidos a procesamiento histológico de rutina. Los cortes fueron te idos con HE y técnicas histoquímicas para mucinas. El análisis morfométrico mostró la mantención de la pared total y del grosor de la túnica muscular, y reducción en el espesor de la túnica mucosa, en el número de células caliciformes, en la profundidad de las criptas y en la altura de los enterocitos y de sus núcleos, en los animales desnutridos. Los datos obtenidos sugieren que la desnutrición proteica provoca alteraciones en la morfometría intestinal del colon ascendente de ratas adultas, principalmente en tejidos de alto índice de renovación celular como la mucosa y, consecuentemente, de sus estructuras como los enterocitos, células caliciformes y criptas.
Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition Atrofia de la Pared Intestinal y Aumento de la Secreción de Sulfomucinas en el Epitélio Yeyunal de Ratas Sometidas a Intensa Desnutrición Protéica
Cássia Luiza de Marins Franco,Débora de Mello Gon?ales Sant'Ana,Eduardo José de Almeida Araújo
International Journal of Morphology , 2010,
Abstract: The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4μm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium. Fueron evaluados los efectos de la desnutrición proteica severa sobre la morfometría de la pared intestinal del yeyuno de ratas adultas . Para esto, se utilizaron seis ratas (Rattus norvegicus) Wistar, con 90 días de edad, distribuidas en dos grupos: GC (grupo control, n=3) y el GD (grupo desnutrido, n=3). Los animales del GC recibieron ración con un contenido proteico de 26% y los del GD ración con 4% de proteínas. Después de 90 días de experimentación, parte del yeyuno fue retirado y sometido a un procesamiento histológico de rutina. Cortes transversales de 4 μm fueron te idos con HE y técnicas histoquímicas (Periodic Acid Schifff (PAS) y Alcian Blue (AB) pH 2,5 y pH 1,0.) para evidenciar glicoconjugados. El análisis morfométrico te idos con HE demostró reducción en el grosor total de la pared del yeyuno, especialmente de las túnicas muscular y mucosa, además se observó una disminución en la altura de los entericitos. Los cortes te idos con técnicas histoquímicas revelaron que el número de células caliciformes reactivas al PAS y al AB pH 2,5 se mantuvo; por otro lado, hubo un aumento en el número de células reactivas al AB pH 1,0. Se concluye que la desnutrición proteica severa provoca atrofia de la pared yeyunal y aumento de la secreción de sulfomucinas en el epitelio intestinal.
Atrophy of the Nitrergic Myenteric Neurons in the Descending Colon Rats Submitted to Protein and Vitamin Deficiency
Araújo,Eduardo José de Almeida; Hermes,Catchia; Miranda Neto,Marcílio Hubner de; Almeida,Elton Carlos de; Sant'Ana,Débora de Mello Gon?ales;
International Journal of Morphology , 2009, DOI: 10.4067/S0717-95022009000300047
Abstract: effects of protein and b-complex vitamin deficiency were assessed with respect to the morphometry of myenteric neurons in the descending colon of adult rats. sixteen animals were divided into two groups: control group (cg, n=8) and experimental group (eg, n=8). the cg received 22% protein chow and the eg received 4% protein chow for 120 days. the descending colon was submitted to nadh- and nadph-diaphorase technique in order to evidence nervous cells in the whole mounts preparations. in the eg, nadh-d positive neurons presented reduced nuclei, while nadph-d positive neurons showed atrophy of the soma area (~41.7%) inducing an increase of the proportion occupied by the nucleus inside in the soma of these cells.
Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency
Moreira, Neide Martins;Hermes, Catchia;Almeida, Carla Simone Leite de;Santana, Evelyne Cruz;Sant'Ana, Débora de Mello Gon?ales;Araújo, Eduardo José de Almeida;
Arquivos de Neuro-Psiquiatria , 2008, DOI: 10.1590/S0004-282X2008000200020
Abstract: the effects of protein malnutrition on the quantitative aspects of the myenteric plexus in the ileum of adult rattus norvegicus were assessed. thirty 90-day-old rats were divided into two groups: control group (cg, n=15) and experimental group (eg, n=15). the cg received 26% protein chow and the eg received 4% protein chow for 90 days. at the end of the experiment, the animals from the cg weighed 369.63±26.33, and the ones from the eg 215.34±56.31. the ileum was submitted to giemsa, nadh- and nadph-diaphorase technique in order to evidence nervous cells in the whole-mount preparations. animals from the eg presented a 41.75% body weight loss in relation to the cg as well as 17.6% length reduction for the ileum-jejunum. moreover, the organ was 41% lighter for the eg. giemsa-stained neurons were 17.02% more concentrated in the eg (p>0.05). nadh-diaphorase-stained neurons were 26.6% more concentrated in the eg (p<0.05), while the nadph-diaphorase were 26.28% more concentrated in this group (p<0.05).
Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats
Alves,Marcelo Sardeto; Silva,Aristeu Vieira da; Bianchi,Larissa Renata de Oliveira; Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gon?ales;
International Journal of Morphology , 2011, DOI: 10.4067/S0717-95022011000100048
Abstract: the aim of the study was quantifying and morphologically analyzing the myenteric neurons of the small and large gastric curvatures of the glandular stomach of rats infected the tachyzoites of the toxoplasma gondii for 30 days. ten male rats were assigned into two groups: control group (cg) and experimental group (eg). the animals from the cg received saline solution orally whereas the eg animals received 104 tachyzoites of the t. gondii genotype iii strain (btu ii). after 30 days, euthanasia was conducted for the removal of the stomach, which was dissected under the stereomicroscope for removal of the tunica mucosa and the tela submucosa. whole mounts were stained with giemsa. quantification of the myenteric neurons was conducted by using a 40x-objective microscope in 40 microscopic fields for the region of the small gastric curvature and 40 fields for the large gastric curvature of the glandular stomach of the animals from both groups. the cell body of 50 myenteric neurons from each region was measured for each animal. chronic experimental infection caused by the genotype iii strain of toxoplasma gondii was verified to reduce myenteric neuron density only in the small gastric curvature region of the glandular stomach, not resulting in significant changes in the size of the neurons.
Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency
Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gon?ales; Molinari,Sonia Lucy; Miranda Neto,Marcílio Hübner de;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000500013
Abstract: we studied the effects of a severely hypoproteic diet on the quantitative aspects of the myenteric plexus of the descending colon of young rats. eighteen rats were divided into two groups, one of them being fed with a chow having 26% protein (control) and the other with a chow having 4% protein, balanced for minerals and vitamins, during 12 weeks. the whole-mounts of the descending colon had their myenteric neurons stained either with giemsa or nadph diaphorase. the rats from the experimental group had deficits of body weight (54.23%) and area of the descending colon (48.14%); additionally, we observed that there was no alteration in the total number of neurons of the colon, but a decrease in the number of nadph-diaphorase positive neurons (37.80%). the implications of these results concerning the priority that some cellular types may have when nutrients are less available are discussed
Effect of protein and vitamin B deficiency on the morpho-quantitative aspects of the myenteric plexus of the descending colon of adult rats
Araújo, Eduardo José de Almeida;Sant'Ana, Débora de Mello Gon?ales;Molinari, S?nia Lucy;Miranda Neto, Marcílio Hubner de;
Arquivos de Neuro-Psiquiatria , 2003, DOI: 10.1590/S0004-282X2003000200012
Abstract: we carried out this work with the purpose of studying the effects of protein and vitamin b deficiency on the morphologic and quantitative aspects of the myenteric plexus of the descending colon of adult rattus norvegicus. twenty-eight rats were divided in two groups, one of them receiving chow with 22% protein level (control) and the other fed with chow having 8% protein level without vitamin b supplementation, during 120 days. whole-mounts of the descending colon were prepared and stained with giemsa, nadh-diaphorase and nadph-diaphorase. the undernourished rats had a body weight 11.84% less than the control group. relative to the controls, the experimental group had a colonic area 48% smaller, 51.9% less giemsa-stained neurons, 28.3% less nadh-diaphorase positive neurons and 24.2% less nadph-diaphorase positive neurons.
Regional differences in the number and type of myenteric neurons in the descending colon of rats
Araújo, Eduardo José de Almeida;Sant'Ana, Débora de Mello Gon?ales;Molinari, S?nia Lucy;Miranda Neto, Marcílio Hubner de;
Arquivos de Neuro-Psiquiatria , 2003, DOI: 10.1590/S0004-282X2003000200011
Abstract: the purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. whole-mounts were stained with three different techniques of neuronal evidenciation. through counts of the number of neurons in an area of 6.64 mm2 under light microscopy, we found 1,271 ± 227.54 neurons with giemsa in the intermediate region and 1,234 ± 225.92 neurons in the antimesocolic region; with the nadh-diaphorase technique we found 530 ± 92.97 neurons in the intermediate region and 539 ± 146.72 neurons in the antimesocolic region; and through the nadph-diaphorase histochemistry, we found 417 ± 34.42 neurons in the intermediate region and 547 ± 84.01 neurons in the antimesocolic region. we conclude that there is a variation in the density of nadph-diaphorase positive neurons in the intestinal circumference; that the nadh-diaphorase positive neuronal subpopulation represented 42.7% of that stained with giemsa; and that the nadph-diaphorase positive neurons represented 37.8% of the whole myenteric population.
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