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Search Results: 1 - 10 of 6472 matches for " Edisson; Gil-Villa "
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Evaluation of atypical semen parameters in individuals whose couples had a history of early recurrent embryo death: in search for a reference value Evaluación de parámetros seminales no convencionales en individuos cuyas parejas presentan muerte embrionaria temprana recurrente: en busca de un valor de referencia
Edisson Rodríguez,Aura María Gil-Villa,Daniel Camilo Aguirre-Acevedo,Walter Cardona-Maya
Biomédica , 2011,
Abstract: Introduction. Previous studies related alterations in non-conventional seminal parameters with recurrent early embryonic death for one couple. A reference standard of clinical assessment is required for the management of these kinds of patients. Objective. Normal semen parameters were established based on functional tests including lipid peroxidation of sperm membranes, antioxidant capacity of seminal plasma and integrity of sperm chromatin to compare with men whose partners have recurrent early embryonic death. These parameters set reference values to identify subfertile individuals whose condition can be attributed to altered semen parameters. Materials and methods. The conventional and non-conventional semen parameters of 47 samples of semen were evaluated. Thirty-six samples were from subfertile individuals whose partners had a history of early recurrent embryo death, and 11 samples were from individuals with recent evidence of normal fertility. Results. By discriminant analysis, the two groups were classified as follows: a value below 0.50 for 86.1% of individuals in the group of recurrent early embryonic death, and a value above 0.50 to classify 81.8% of individuals in the group of recent fertility. Conclusions. This reference value of 0.5 based on the results of sperm tests can identify infertile male patients whose partners have a history of early embryonic death. This will aid the physician to suggest treatments more focused on the possible cause of subfertility. Introducción. En estudios previos se relacionaron las alteraciones en los parámetros espermáticos no convencionales con la presencia de muerte embrionaria temprana recurrente de la pareja; debido a esto, se planteó la necesidad de establecer un valor de referencia de utilidad clínica para el manejo de estos pacientes. Objetivo. Evaluar los parámetros seminales convencionales y las pruebas funcionales de lipoperoxidación de las membranas espermáticas, capacidad antioxidante del plasma seminal e integridad de la cromatina espermática, en individuos cuyas parejas presentan muerte embrionaria temprana recurrente, con el fin de obtener un valor de referencia y poder identificar aquellos individuos en quienes la disminución de la fertilidad se pueda demostrar con estas pruebas. Materiales y métodos. Se evaluaron los parámetros espermáticos convencionales y no convencionales de 47 muestras de semen, de las cuales, 36 pertenecían al grupo de individuos con disminución de la fertilidad por historia de muerte embrionaria temprana recurrente en sus parejas, y 11 muestras de individuos sanos con fe
Evaluación de parámetros seminales no convencionales en individuos cuyas parejas presentan muerte embrionaria temprana recurrente: en busca de un valor de referencia
Rodríguez,Edisson; Gil-Villa,Aura María; Aguirre-Acevedo,Daniel Camilo; Cardona-Maya,Walter; Cadavid,ángela P;
Biomédica , 2011,
Abstract: introduction. previous studies related alterations in non-conventional seminal parameters with recurrent early embryonic death for one couple. a reference standard of clinical assessment is required for the management of these kinds of patients. objective. normal semen parameters were established based on functional tests including lipid peroxidation of sperm membranes, antioxidant capacity of seminal plasma and integrity of sperm chromatin to compare with men whose partners have recurrent early embryonic death. these parameters set reference values to identify subfertile individuals whose condition can be attributed to altered semen parameters. materials and methods. the conventional and non-conventional semen parameters of 47 samples of semen were evaluated. thirty-six samples were from subfertile individuals whose partners had a history of early recurrent embryo death, and 11 samples were from individuals with recent evidence of normal fertility. results. by discriminant analysis, the two groups were classified as follows: a value below 0.50 for 86.1% of individuals in the group of recurrent early embryonic death, and a value above 0.50 to classify 81.8% of individuals in the group of recent fertility. conclusions. this reference value of 0.5 based on the results of sperm tests can identify infertile male patients whose partners have a history of early embryonic death. this will aid the physician to suggest treatments more focused on the possible cause of subfertility.
Muerte embrionaria temprana: ?Tiene influencia el factor masculino?
Gil Villa,Aura María; Cardona-Maya,Wálter Darío; Cadavid Jaramillo,ángela Patricia;
Archivos Espa?oles de Urología (Ed. impresa) , 2007, DOI: 10.4321/S0004-06142007000900002
Abstract: objective: to discuss the possible role of the male factor in early embryo death. method: a detailed bibliographic review has been put together to establish which alterations in spermatozoa can be associated with early embryo death. results: before the fusion between plasma membranes of the sperm and the oocyte occurs, both germ cells must undergo a maturation process that allows successful fertilization and embryo development. the study of couples with early embryo loss is usually approached from the side of the woman due to the obvious relationship that exists between the female and the developing embryo. however, it is not illogical to suppose that a genetic or epigenetic alteration of the sperm could have important consequences on these losses due to the necessary contribution of the male gamete not only to embryonic but also to placental development. on the other hand, spermatozoa have certain characteristics such as a highly compact dna, they undergo apoptosis and the seminal plasma contains antioxidants that protect the structural and functional integrity of the germ cell. these factors assure fertilization and embryo development. nevertheless, epigenetic alterations of the sperm such as altered chromatin packing, mistakes in imprinting, absence or alteration of the centrosome, telomeric shortening and absence of sperm rna, could affect functions leading to early embryo loss. conclusions: knowledge concerning sperm intervention previous to embryo development will provide the basis for better understanding and for possible diagnosis and treatment of diverse reproductive alterations in men that could impede embryo development.
Uso de triamcinolona intravítrea en el tratamiento del edema macular diabético difuso persistente
García Fernández,M.; García Alonso,A.; Fonollá Gil,M.; Rodríguez Villa,S.;
Archivos de la Sociedad Espa?ola de Oftalmología , 2011, DOI: 10.4321/S0365-66912011001000002
Abstract: purpose: to determine the efficacy of intravitreal triamcinolone injections (iv ta) for diffuse persistent diabetic macular oedema (dmo) based on the functional parameter of modification in best corrected visual acuity (bcva) and the anatomic parameter of quantitative changes in central macular thickness, as determined by optical coherence tomography (oct). the secondary outcome is to analyse the safety of the procedure. methods: in this retrospective study, 16 patients (22 eyes) were included over a period of six months. type and time of evolution of diabetes mellitus, previous treatments, bcva, lens status, intraocular pressure (iop) and central macular thickness, were analysed. during the follow-up period were collected: number of injections, changes in bcva, iop, central macular thickness, and complications observed. results: improvement in bcva was recorded in 30.77%, 47.37% and 52.63%, at one, three and six months, respectively (p<.05 at 3 months). the iop increased in 57.69% at one month, and 75 and 47.05%, at 3 and 6 months, respectively (p<.05 at 3 months). progression of cataracts was found in 22.72%. no cases of endophthalmitis were observed. conclusions: intravitreal ta is a good therapeutic option for patients with persistent dmo, increasing bcva and decreasing central macular thickness in the short term, with a percentage of clinical resolution of more than 70%. however, due to the transient effect, and potential adverse effects, it should be administered to selected refractory cases with caution.
TENDENCIAS POBLACIONALES EN ALGUNAS ESPECIES DE AVES ACUáTICAS EN LA LAGUNA DEL OTúN ENTRE 1998 Y 2007
Gil-Ospina,Raul Fernando; Bedoya-Zuluaga,Felipe Alberto; Casta?o-Villa,Gabriel Jaime;
Boletín Científico. Centro de Museos. Museo de Historia Natural , 2010,
Abstract: based on census conducted in 1998, 2000 and 2007 to anas flavirostris, oxyura jamaicensis and fulica sp. (coots) in the el otún lake (colombian central andes), the trends of population size of these species were described. it was determined that the populations of o. jamaicensis and coots were reduced between 1998 and 2007 in a 63 and 54% respectively, and that the variations in the lake level during 2000 were correlated with the changes in the abundance of a. flavirostris. the results suggest that these species change in abundance both intra and inter annually and that they could be related with environmental variations. on the other hand, some disturbances (the fire occurred in 2006) could have affected the state of these populations reducing them even more. it is a priority to continue with the population monitoring of these species because of their reduced population size which makes them more vulnerable to stochastic environmental and demographic factors.
Absceso esplénico amebiano Amebic splenetic abscess
Lázaro Fidel Gil Manrique,Caridad Soler Morejón,Lourdes Crespo Acebal,Miriam Villa Valdés
Revista Cubana de Cirugía , 2011,
Abstract: La localización extraintestinal es una complicación temible de la amebiasis intestinal, con una elevada mortalidad, que oscila entre el 4 al 14 % de los casos diagnosticados. La forma de presentación más común es el absceso hepático amebiano, y aunque se han reportado localizaciones pulmonares, peritoneales, y hasta cerebrales, la localización esplénica es extremadamente infrecuente. Se reporta un caso de amebiasis esplénica que evolucionó tórpidamente, a pesar de del tratamiento específico con metronidazol desde su admisión al Departamento de Emergencia, y que fue intervenido quirúrgicamente al séptimo día, con evolución favorable después de la resección del bazo. Se realiza una revisión de la literatura actualizada sobre el tema. The extraintestinal location is a fearsome complication of the intestinal amebiasis, with a high mortality fluctuating between the 4 and the 14 % of cases diagnosed. The commonest presentation way is the amebic hepatic abscess and although others have reported pulmonary, peritoneal and up to cerebral locations, the splenetic one is extremely infrequent. A case of splenetic amebiasis is reported which evolved in a torpid way despite the specific treatment with metronidazole from its admission to Emergence Department and that was operated on at seventh day with a favorable evolution after spleen resection. Authors made a review of the updated literature on this subject.
Uso de triamcinolona intravítrea en el tratamiento del edema macular diabético difuso persistente Intravitreal triamcinolone acetonide use in diffuse persistent diabetic macular edema
M. García Fernández,A. García Alonso,M. Fonollá Gil,S. Rodríguez Villa
Archivos de la Sociedad Espa?ola de Oftalmología , 2011,
Abstract: Propósito: Explorar la eficacia de las inyecciones de triamcinolona intravítrea (TA iv) en el edema macular diabético (EMD) difuso persistente, según el parámetro funcional de la modificación en la mejor agudeza visual corregida (MAVC) y el parámetro anatómico del cambio cuantitativo en el espesor foveal central, determinado por tomografía de coherencia óptica (OCT). El objetivo secundario es analizar la seguridad del procedimiento. Métodos: En este estudio retrospectivo se incluyeron 16 pacientes (22 ojos), en un periodo de seis meses. Se analizó tipo y tiempo de evolución de la diabetes mellitus, tratamientos previos, MAVC, estado del cristalino, presión intraocular (PIO) y espesor macular central. A lo largo del seguimiento se recogieron datos relativos al número de inyecciones, así como a cambios en MAVC, PIO, grosor foveal, y complicaciones observadas. Resultados: Se produjo mejoría en la MAVC en el 30,77, 47,37 y 52,63%, al mes, 3 y 6 meses, respectivamente (p<0,05 a los 3 meses). Se observó incremento de la PIO en el 57,69% al mes, y en el 75% y 47,05%, a los 3 y 6 meses (p<0,05 a los 3 meses). Se cuantificó una disminución en el grosor foveal al mes, 3 y 6 meses (p<0,05). Se observó progresión de catarata en 22,72%. No se apreció ningún caso de endoftalmitis. Conclusiones: La TA iv es una buena opción terapeútica para los pacientes con EMD persistente, mejorando la MAVC y reduciendo el grosor macular a corto plazo, con porcentajes de resolución clínica de más del 70%. Sin embargo, su efecto transitorio y sus potenciales efectos adversos hacen que deba administrarse en casos refractarios con precaución. Purpose: To determine the efficacy of intravitreal triamcinolone injections (iv TA) for diffuse persistent diabetic macular oedema (DMO) based on the functional parameter of modification in best corrected visual acuity (BCVA) and the anatomic parameter of quantitative changes in central macular thickness, as determined by optical coherence tomography (OCT). The secondary outcome is to analyse the safety of the procedure. Methods: In this retrospective study, 16 patients (22 eyes) were included over a period of six months. Type and time of evolution of diabetes mellitus, previous treatments, BCVA, lens status, intraocular pressure (IOP) and central macular thickness, were analysed. During the follow-up period were collected: number of injections, changes in BCVA, IOP, central macular thickness, and complications observed. Results: Improvement in BCVA was recorded in 30.77%, 47.37% and 52.63%, at one, three and six months, respectively (P<.05 at 3 m
Produ o de xarope de a úcar invertido obtido por hidrólise heterogênea, através de planejamento experimental
RODRIGUES Marili Villa Nova,RODRIGUES Rodney Alexandre Ferreira,SERRA Gil Eduardo,ANDRIETTA Sílvio Roberto
Ciência e Tecnologia de Alimentos , 2000,
Abstract: A utiliza o de a úcar líquido na indústria alimentícia constitui em vantagem nas aplica es onde o a úcar é adicionado em solu o. O xarope de a úcar invertido reúne a elevada solubilidade da frutose à difícil cristaliza o da glicose, aumentando seu poder edulcorante e diminuindo os riscos de cristaliza o. Essas propriedades contribuem para aumentar o valor desses xaropes para uso em vários produtos alimentícios, sobretudo na indústria de refrigerantes. O produto de maior interesse comercial é aquele com nível de invers o próximo a 55%, pois nessa faixa a solubilidade é máxima possibilitando trabalhar com concentra es em torno de 76,5% de sólidos solúveis, diminuindo assim a susceptibilidade à contamina o microbiana, sem riscos de cristaliza o. O objetivo deste trabalho foi desenvolver as etapas de produ o do xarope de a úcar invertido produzido a partir de resinas de troca-i nica, visando obter um produto final de alta qualidade que atenda às necessidades da indústria de refrigerantes, utilizando como ferramenta o planejamento experimental. O processo de obten o do xarope invertido foi iniciado com a descolora o do xarope de sacarose através de duas colunas contendo resina ani nica, para ent o ser invertido pela elui o numa coluna de resina cati nica. O produto obtido foi isento de qualquer obje o ao paladar, inodoro, praticamente livre de cor (56 ICUMSA) e hidroximetil furfural (11ppm). A contagem microbiológica para fungos, leveduras e bactérias foi menor que 1 unidade/mL.
Influencia de la impronta genómica masculina en la reproducción
Vasco,G.C.; Gil Villa,A.M.; Piedrahita Ochoa,C.; Cardona Maya,W.; Cadavid Jaramillo,A.;
Actas Urológicas Espa?olas , 2008, DOI: 10.4321/S0210-48062008001000009
Abstract: objective: genomic imprinting is the epigenetic change that occurred differentially in the specific genes in spermatozoa and oocyte according to their paternal or maternal origin, thus allowing a monoallelic expression. this review is a critical analysis of the published information relating to the role of the male imprinting on the successful reproduction. methods: we performed a literature search on some of the components that regulate the male genomic imprinting and the possible role on reproductive events such as spermatogenesis, and placental and embryo development. results: the literature analysis allowed us to appreciate structural, genetic and epigenetic changes occurring during the formation of the male gamete that could have an impact on embryo development, mainly in the formation of extra-embryonic tissues as the placenta. conclusion: alterations in the molecular mechanisms involved in the sperm dna methylation during the spermatogenesis, could induce alterations in the normal pattern of expression required in the fetal-placental components development.
Produ??o de xarope de a?úcar invertido obtido por hidrólise heterogênea, através de planejamento experimental
RODRIGUES, Marili Villa Nova;RODRIGUES, Rodney Alexandre Ferreira;SERRA, Gil Eduardo;ANDRIETTA, Sílvio Roberto;FRANCO, Telma Teixeira;
Ciência e Tecnologia de Alimentos , 2000, DOI: 10.1590/S0101-20612000000100020
Abstract: the use of liquid sugar in the food industry constitutes a great advantage in applications where the sugar is added in solution. the invert syrup gathers the high solubility of the frutose to the difficult crystallization of the glucose, increasing its sweetness and decreasing the crystallization risks. those properties contribute to increase the value of those syrups for use in several products, above all in the industry of soft drinks. the objective of this work was improve the stages of invert syrup production using ion exchange resins, seeking to obtain a final product of high quality that assists to the needs of the industry of soft drinks using the experimental design. the production of inverted syrup began with sucrose decolorization process employing two anionic resins columns and subsequent elution through a cationic resin column, which promoted sucrose hydrolysis until inversion level desired. the obtained product was exempted practically of any objection to the palate, scentless, low levels of color (56 icumsa) and hmf (11 ppm). determination of molds, yeasts and bacteria were smaller than 1 unit/ml.
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