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Search Results: 1 - 10 of 35 matches for " Ebensperger "
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Nanoencapsulation of Human Adipose Mesenchymal Stem Cells: Experimental Factors Role to Successfully Preserve Viability and Functionality of Cells  [PDF]
Daniel Hachim, Jaime Melendez, Roberto Ebensperger
Journal of Encapsulation and Adsorption Sciences (JEAS) , 2013, DOI: 10.4236/jeas.2013.31001
Abstract:

Cell nanoencapsulation is a novel delivery system based on a self-assembly technique mediated by electrostatic interactions called Layer-by-Layer (LbL) deposition, without an increase in volume implant because of the nanometric thickness of its layers. LbL coats the entire surface of individual cells, providing mechanical resistance to cells against manipulation and storage conditions prior to implantation in the patient. In this work, single-cell nanocapsule formation using human adipose-derived mesenchymal stem cells (ADSC) given their potentiality in regenerative medicine was assessed by fluorescence microscopy and Zeta potential assays. Both methodologies were conclusive in showing layer-by-layer nanocapsule formation of every single ADSC. Significant differences in terms of viability and cell functionality preservation were observed depending on the polycation used. Using a combination of fluorescence microscopy and fluorimetric assays, we found that cell survival after nanocapsulation was only efficient when chitosan was added to cells. These results were consistent with other cell types used in this study. Other polycations such as poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride) (PDADMAC) and poly-L-lysine (PLL) markedly decreased cell viability (22%, 11% and 15%, respectively). In addition, the use of potassium-enriched saline solutions, such as Hanks and Ringer’s solution, during the nanoencapsulation process on ADSCs was harmful on cell viability compared to standard media (36% vs 79%, respectively). The addition of a mixture of polyanions such as hyaluronic acid and chondroitin sulfate did not affect cell viability (79% and 81%). The combination of chitosan/hyaluronic acid and chondroitin sulfate was also effective in

Dustbathing and intra-sexual communication of social degus, Octodon degus (Rodentia:Octodontidae)
EBENSPERGER,LUIS A.;
Revista chilena de historia natural , 2000, DOI: 10.4067/S0716-078X2000000200011
Abstract: i observed the behavior of captive male and female octodon degus to assess if dustbathing behavior plays a role in social communication among unfamiliar, same-sex conspecifics. degus of a first group (control responders) were individually exposed during 10-min tests to an arena containing loose, clean soil. i compared the latency to first dustbathing as well as the overall frequency of dustbathing events recorded to control responders with the corresponding figures recorded to a second group of degus (experimental responders) after they were individually introduced into the same arena but with soil previously used for dustbathing by a same-sex conspecific (depositor). i also compared the location of dustbathing events by experimental responders with that of depositor individuals. although male degus tended to exhibit shorter latencies to first dustbathing event when in clean soil, this variable was not significantly influenced by sex of responders or the type of soil (clean or used). in contrast, a significant interaction between both factors revealed that males dustbathe at a higher rate than females when on clean soil, but similarly so when in a substratum previously dustbathed by a same-sex conspecific. the place chosen by both male and female responders to conduct their dustbathing behavior was unrelated to the presence of previous marks left by a depositor degu. i conclude that dustbathing is involved in communication during male-male, but not during female-female, interactions in the degu. i suggest that such male-male interactions represent competition for mates
Medición "in situ" de la permeabilidad al aire del hormigón: status quo
Ebensperger,Luis; Torrent,Roberto;
Revista ingeniería de construcción , 2010, DOI: 10.4067/S0718-50732010000300003
Abstract: the authors have been involved in the creation, preliminary tests and development of the "torrent method", which is intended to test air permeability in concrete. it's been more than 15 years since such foundational research and, now, this paper presents a review of the evolution and current situation of the method, included in the switzerland standards in 2003. application examples conducted in laboratory and civil works (bridges, tunnels, and so on) are introduced, including data from different countries worldwide. correlations between the air permeability coefficient kt and other durability indicators, such as chloride migration (astm c1202), penetration of water under pressure (en 12390-8) or capillary action are presented. finally the future prospective uses are discussed, as quality control tool for new structures, considering relevant implications they would lead to, as well as the diagnosis on existing structures.
Pruebas hepáticas en la toxicosis
INES EBENSPERGER,AUGUSTO WINTER
Revista chilena de pediatría , 1953,
Abstract:
CéLULAS MADRES: NUEVAS FRONTERAS PARA LA MEDICINA
ENRIQUE OYARZúN EBENSPERGER
Revista Chilena de Obstetricia y Ginecología , 2005,
Abstract:
Salud materna en las américas
ENRIQUE OYARZúN EBENSPERGER
Revista Chilena de Obstetricia y Ginecología , 2005,
Abstract:
INFORME DEL PRESIDENTE DE LA SOCIEDAD CHILENA DE OBSTETRICIA Y GINECOLOGíA DE LA REUNIóN DEL COMITé EJECUTIVO DE FIGO EN GINEBRA Y DE OTRAS ACTIVIDADES
Enrique Oyarzún Ebensperger
Revista Chilena de Obstetricia y Ginecología , 2004,
Abstract:
EDITORIAL
ENRIQUE OYARZúN EBENSPERGER
Revista Chilena de Obstetricia y Ginecología , 2004,
Abstract:
Abstract
Enrique Oyarzún Ebensperger
Revista Chilena de Obstetricia y Ginecología , 2004,
Abstract:
Sociedad Chilena de Obstetricia y Ginecología, 1935-2005
Enrique Oyarzún Ebensperger
Revista Chilena de Obstetricia y Ginecología , 2005,
Abstract:
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