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Search Results: 1 - 10 of 127001 matches for " Duochuan Li "
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Li Duochuan Shen Chongyao,

微生物学通报 , 1993,
Abstract: Based on the taxonomic system of Nelson et al. (1993) ,a preliminary computerized identification system with groupaverage method of hierachical cluster analysis for the genus Fusarium was established. Three unidentified isolates, Fusarium sp. 1 F. sp. 2 and F. sp. 3 from Zea mays L. , were identified by use of this analysis. The results clearly showed that F. sp. 1 was identified as F. graminearum, F. sp. 2 as F. moniliforme and F. sp. 3 as F. subflutinans or F. proliferatum. The facts proved its applicability to comparison and identification of Fusarium.
Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in thermophilic fungus Thermomyces lanuginosus

Hua Han,Xiaoxue Xu,Yanjie Peng,Dehui Kong,Duochuan Li,

微生物学报 , 2012,
Abstract: 摘要:【目的】建立疏绵状嗜热丝孢菌的稳定遗传转化体系并获得插入突变体。【方法】利用农杆菌介导的方法建立疏绵状嗜热丝孢菌的遗传转化体系;分别通过Southern杂交、克隆转移DNA(T-DNA)侧翼序列来确定T-DNA 在疏绵状嗜热丝孢菌基因组中的拷贝数和插入位点。【结果】成功建立了可靠的疏绵状嗜热丝孢菌的遗传转化体系。共培养过程中使用萌发孢子是成功建立疏绵状嗜热丝孢菌遗传转化体系的必要条件。疏绵状嗜热丝孢菌萌发的孢子与农杆菌在28℃共培养48h时,转化效率最高。乙酰丁香酮(AS)在农杆菌预培养及疏绵状嗜热丝孢菌萌发的孢子与农杆菌的共培养阶段都是必需的,且在共培养阶段当AS浓度为500 μM时转化效率最高。Southern 杂交验证表明,79.2%的转化子为T-DNA单拷贝插入,且通过热不对称PCR(TAIL-PCR)分析得出T-DNA在该菌基因组中的插入位点是随机的。通过该转化系统筛选到部分表型突变体。【结论】我们首次报道了利用ATMT技术成功转化嗜热真菌-疏绵状嗜热丝孢菌,证明了该方法是一种简单有效的获得插入突变体的方法,并为该嗜热真菌进行基因定位提供了工具。
Beneficial Experience from Teaching and Education to Research and Development  [PDF]
Li Li
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B039

Teaching and Education (T&E) constitute the most important activity in knowledge transfer from generation to generation. This can explain why government organizations consider the training of highly qualified personnel as one of the most important criteria in the selection of research and development (R&D) grant applications. A university professor should thus not only play the role of researcher, but also that of teacher. T&E and R&D combine to form an inseparable relationship for university professors. By shooting for excellence in T&E, we could get a new perception of a familiar field or initiate a brand new field altogether, which would in turn enhance our research. The quest for excellence in R&D leads to deeper and better understanding of materials taught, and progress in R&D enriches our T&E endeavors. Here, the author shares a beneficial experience from T&E to R&D.

A Causality Analysis of Coal Consumption and Economic Growth for China and India  [PDF]
Jinke Li, Zhongxue Li
Natural Resources (NR) , 2011, DOI: 10.4236/nr.2011.21007
Abstract: China and India are the two countries with the strongest economic growth in the world. Meanwhile they consume much of the global coal to fuel their economic development. With coal burning as a major factor contributing to global greenhouse gas emissions, China and India are confronted with a dilemma of economic growth and environment protection. Will coal consumption reduction cause economic shocks? Is there a causal relationship between coal consumption and economic growth in China and India? In this paper Granger causality tests were used to examine the relationship between coal consumption and GDP in China and India, using data for the period from 1965 to 2006. It was found that a unidirectional causality from GDP to coal consumption existed in China while a unidirectional causality from coal consumption to GDP did in India. Therefore, developing cleaner and more efficient technologies is essential to reduce their CO2 emissions to reach sustainable development.
An Investigation and Analysis of Information Overload in Manager's Work  [PDF]
Tao Li, Min Li
iBusiness (IB) , 2011, DOI: 10.4236/ib.2011.31008
Abstract: The problem of information overload in management field has been receiving increasing attention, while there are less empirical researches now. This paper proposes a conceptual model and some operational variables, in which information overload is as an intervening variable between the factors of causes and impacts. An investigation and analysis has been conducted from a selected sample of managers. This research suggests that work efficiency and work quality are both not strongly connected with information overload. We also find that the main causes of information overload are the work interruption and work time pressure but the amount of information.
Selection Advantage Pattern of International Sci-Tech Cooperation and Empirical Analysis  [PDF]
Li Li, Bin Yu
Journal of Service Science and Management (JSSM) , 2011, DOI: 10.4236/jssm.2011.41006
Abstract: Traditional international sci-tech cooperation theories mainly focus on the ability and advantage owned by cooperative parties, and according to which to guide the direction of international science and technology cooperation. Based on the integration of traditional theories of international science and technology cooperation and concrete practice, this paper proposes the selection advantage pattern and matching mechanism, which comprehensively take cooperator advantages, specification of project and change in circumstance into account and will play a guiding role in terms of determining the key country or region with which to prior carry out international science and technology cooperation. This paper also gives an analysis of the current status of industry development in Guangdong province and the research upon competitiveness evaluation of the world's related leading technology, puts forward corresponding countermeasures and suggestions on the science and technology cooperation patterns and approaches in terms of technological level and resources of Guangdong province.
Soil reinforcement by a root system and its effects on sediment yield in response to concentrated flow in the loess plateau  [PDF]
Peng Li, Zhanbin Li
Agricultural Sciences (AS) , 2011, DOI: 10.4236/as.2011.22013
Abstract: The importance of roots in soil conservation has long been underestimated due to a lack of sys-tematic studies conducted to evaluate root dis-tribution patterns and their effects on soil ero-sion. Current knowledge regarding root mor-phology and its impact on soil erosion by water is limited; therefore, detailed analysis of the role that root systems play in controlling soil ero-sion is needed. In this study, stratified runoff scouring at different soil depths in the field was conducted in a grassland area. The results in-dicated that both root biomass and soil wa-ter-stable aggregates decreased as soil depth increased at all three sites, while there was al-most no change in soil bulk density at 1.3g/cm3. Sediment yields under different runoff dis-charge at different sites showed similar trends, and the sediment yield increased as the soil depth increased at all three sites. Further analysis revealed that close relationships ex-isted between root biomass and the amount of water-stable aggregates and soil organic matter content, and that these factors greatly influ-enced soil erosion. Based on the data generated by the experiment, equations describing the relationship between sediment production at different soil depths and root biomass were determined.
Second-Order Volition and Conflict between Desires  [PDF]
Hengxi Li, Hengwei Li
Open Journal of Philosophy (OJPP) , 2012, DOI: 10.4236/ojpp.2012.21004
Abstract: In Freedom of the Will and the Concept of a Person, Harry Frankfurt put forward a theory that what is essential to be a person is second-order volition. The notion of second-order volition can be used as a key conceptual tool in understanding the conflict between desires. By means of the notion, this paper argues that the conflict between desires in our minds lies in the conflict between second-order volitions, other than the conflict between first-order desires. Based on this claim, this paper suggests that, due to the misunderstanding of the nature of the conflict between desires, the analysis of unwilling addict and wanton addict given by Frankfurt is thus wrong, and in his follow-up articles he made wrong description of the phenomenon concerning the conflict between desires.
A Fixed-Phase Quantum Search Algorithm with More Flexible Behavior  [PDF]
Xin Li, Panchi Li
Journal of Quantum Information Science (JQIS) , 2012, DOI: 10.4236/jqis.2012.22006
Abstract: When the Grover’s algorithm is applied to search an unordered database, the probability of success usually decreases with the increase of marked items. To address this phenomenon, a fixed-phase quantum search algorithm with more flexible behavior is proposed. In proposed algorithm, the phase shifts can be fixed at the different values to meet the needs of different practical problems. If research requires a relatively rapid speed, the value of the phase shifts should be appropriately increased, if search requires a higher success probability, the value of the phase shifts should be appropriately decreased. When the phase shifts are fixed at , the success probability of at least 99.38% can be obtained in iterations.
Game Analysis of Institutional Investors Participating in Corporate Governance  [PDF]
Yingzhao Li, Zhenqin Li
American Journal of Industrial and Business Management (AJIBM) , 2013, DOI: 10.4236/ajibm.2013.31008

By building a game model between the institutional investors and the management, an analysis has been conducted to uncover the influential factors that are crucial to the role switching of institutional investors when confronting tunneling behaviors of the management: supervision cost, shareholding ratio, invisible income, fines and patience. In cases of lower supervision cost, higher shareholding ratios, less invisible income, larger amount of fines, more patience and pursuing long-term gains, institutional investors will tend to play an active role in corporate governance. They will act as an active supervisor to restrain the tunneling behavior of the management.

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