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Search Results: 1 - 10 of 180162 matches for " Dulcinéia Beirigo de Souza "
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Dulcinéia Beirigo de Souza
Nuances : Estudos sobre Educa??o , 2011,
Interna o por ordem judicial: dilemas éticos vivenciados por enfermeiros Internación por orden judicial: dilemas éticos vividos por enfermeros Hospitalization by court order: ethical dilemmas experienced by nurses
Mara Ambrosina de Oliveira Vargas,Flávia Regina Souza Ramos,Dulcinéia Ghizoni Schneider,Nadir Schneider
Revista Gaúcha de Enfermagem , 2013,
Abstract: Pesquisa qualitativa, cujo objetivo foi descrever as situa es vivenciadas e os dilemas éticos dos enfermeiros no percurso de encaminhamento e recebimento, por ordem judicial, de pacientes com indica o de interna o em Unidade de Terapia Intensiva (UTI). Foi efetivada entrevista parcialmente estruturada com 10 enfermeiros, que atuam em UTI e 10 que atuam em emergência de hospitais públicos e privados da regi o metropolitana de Porto Alegre, Brasil. Os dados foram submetidos à análise temática. Os resultados indicam que os enfermeiros vivenciam dilemas éticos consequentes aos problemas de superlota o das UTI e das emergências, da precária tecnologia especializada, da orienta o quanto ao benefício concedido pela lei. Concluiu-se que é fundamental a participa o dos enfermeiros em debates que possibilitem mapear as diferentes instancias que têm promovido esta situa o, muitas vezes caótica. Investigación cualitativa cuyo objetivo fue describir las situaciones vividas y los dilemas éticos de los enfermeros en el transcurso del envío y recibimiento por orden judicial de pacientes con indicación de internamiento en la Unidad de Cuidados Intensivos (UCI). Se realizó una entrevista parcialmente estructurada con 10 enfermeros que actuaban en la UCI y 10 que actuaban en Emergencias de hospitales públicos y privados de la región metropolitana de Porto Alegre, Brasil. Los datos fueron analizados según el Análisis Temático. Los resultados indican que los enfermeros experimentan dilemas éticos consiguientes problemas de hacinamiento en emergencia y UCI, especialista en tecnología pobre, la orientación en cuanto a los beneficios establecidos por la ley. Se concluye que es fundamental la participación de los enfermeros en debates que posibiliten mapear las diferentes instancias que han promovido esta situación muchas veces caótica. A qualitative study aimed at describing the situations experienced and the ethical dilemmas of nurses in the process of referring and receiving hospitalized patients by court order who require admission to the Intensive Care Unit (ICU). A partially structured interview was conducted with 10 nurses who worked in the ICU and 10 who worked in the Emergency Room (ER) in public and private hospitals in the metropolitan area of Porto Alegre, Brazil. The data was analyzed following the Semantic Analysis. The results indicated that nurses experienced ethical dilemmas associated with problems of overcrowding in emergency rooms and ICUs, poor specialized technology and orientation as to the benefits provided by law. We concluded that it is essentia
Landscape-Level and Fine-Scale Genetic Structure of the Neotropical Tree Protium spruceanum (Burseraceae)
Fábio de Almeida Vieira,Cristiane Gouvêa Fajardo,Anderson Marcos de Souza,Dulcinéia de Carvalho
International Journal of Forestry Research , 2010, DOI: 10.1155/2010/120979
Abstract: Knowledge of genetic structure at different scales and correlation with the current landscape is fundamental for evaluating the importance of evolutionary processes and identifying conservation units. Here, we used allozyme loci to examine the spatial genetic structure (SGS) of 230 individuals of Protium spruceanum, a native canopy-emergent in five fragments of Brazilian Atlantic forest (1 to 11.8 ha), and four ecological corridors (460 to 1?000?m length). Wright's statistic and Mantel tests revealed little evidence of significant genetic structure at the landscape-scale ( ; , ). At fine-scale SGS, low levels of relatedness within fragments and corridors ( , ) were observed. Differences in the levels and distribution of the SGS at both spatial scales are discussed in relation to biological and conservation strategies of corridors and forest fragments. 1. Introduction Forest loss and spatial isolation of natural populations affect negatively the reproductive success of many tropical plants [1, 2]. In contrast, some studies have shown that the habitat fragmentation had resulted in facilitated pollen movement [3, 4], whilst gene flow measured among populations of several insect-pollinated woody plant species [5, 6] and in a wind-pollinated tree species [7] was not reduced, facilitating too the long distance dispersal and high genetic diversity of remnant populations [8]. However, when comparing continuous and naturally fragmented populations in a tropical tree species, fragmented populations exhibited slightly lower genetic diversity [9]. Landscape structures, such as ecological corridors, are then an important alternative in the demographic and genetic connection of isolated forest fragments, thus minimizing the negative effects of habitat fragmentation [10]. Nonetheless, most studies deal only with the use of corridor’s importance for fauna conservation [11]; little is known about corridor importance for plant species mainly on genetic processes [12]. The genetic differentiation in fragment/corridor may be quantified with indirect methods which are based on the structure of neutral genetic diversity amongst populations, contributing to the knowledge of how landscape characteristics structure populations [13]. Moreover, studies of spatial genetic structure (SGS) provide insight into micro-evolutionary patterns by elucidating the movement of genes at a range of spatial scales [14]. At landscape-level scales between natural populations, genetic structure has been attributed to historical factors and isolation by distance [15]. At fine-scale SGS, seed
Anderson Marcos de Souza,Dulcinéia de Carvalho,Fábio de Almeida Vieira,Leandra Helena do Nascimento
CERNE , 2007,
Abstract: Calophyllum brasiliense Camb. (Clusiaceae) is a tree of great ecological plasticity and it is present in different forestphysiognomy. Due to its preference to water-saturated soils, it is considered specialist in habitat, due to this, it occurs frequently inthe riparian forest. In order to access and understand the inter and intrapopulation genetic variability patterns, two populations of C.brasiliense in gallery forest were sampled. The results obtained by isoenzyme electrophoresis analysis showed a high heterozygosityfor this species, equal 0.444 and 0.492. The genetic structure analysis indicated the absence of intra and inter populationsinbreeding ( f = -0.078; F = -0.063). Most of the genetic variability was distributed within the populations ( = 0.14) and the geneflow was low ( m N= 0.83). The coancestry coefficient estimated showed positive spatial structure in small distance classes. Thisinformation is important to programs of conservation genetics in situ and ex situ of the species. Moreover, it is necessary to improvethe preservation and the maintenance of natural populations of C. brasiliense, since this species demonstrates fragility to factors thatput at risk its genetic variability. op
Anderson Marcos de Souza,Dulcinéia de Carvalho,Sebasti?o Carlos da Silva Rosado,Nilza de Lima Pereira Sales
CERNE , 2001,
Abstract: Pisolithus spp. is a cosmopolitan fungus living in symbiosis with a number of tree species, mainly Eucalyptus and Pinus. Isolates from Brazil, USA and Canadá were used for theevaluation of the diversity of Pisolithus spp. Morphological characteristics and genetic marker wereanalysed for the identification of potential genotypes with broad genetic base that could be used in programs aiming at improving the ectomycorrhizal symbiosis. It was possible to group the isolates onthe basis of morphological characteristics and genetic marker. The divergence rate for morphologicalcharacteristics ranged from 0 to 82% and for enzyme from 0 to 79%. The crosses apt to broaden thegenetic base for morphological characteristics were P4 x P21 and P4 x P25 and enzymes were P13 xP18 and P15 x P33.
Nilza de Lima Pereira Sales,Sebasti?o Carlos da Silva Rosado,Dulcinéia de Carvalho,Anderson Marcos de Souza
CERNE , 2003,
Abstract: This research developed a strategy for genetic improvement of theectomycorhizal f ungus Pisolithus microcarpus aiming at producing commercial innoculumfor Eucalyptus seedlings. After mapping the spatial and temporal distribution of fruitingbodies in two experimental plots of a Eucalyptus understory, basidiospores were collectedfrom six basidiomata and germinated to produce parental monokaryons isolates in vitro.After the identification of the sexual compatibility system of monokaryons, some compatiblesparental isolates were crossed in vitro to produce hybrid dikaryotic progenies and to developa model applicable to a diallell mating design. The diallell analyses were done for de trait ofmycelia growth. The use of such model allowed the estimation of the values of GeneralCombining Ability (GCA) of each parental monokaryons isolates and the values of SpecificCombining Ability (SCA), for each one of the crossing system between parentalmonokaryons. These combining abilities showed the potential use of basiospores to producecommercial innoculum.
Estimativas de parametros genéticos e fenotípicos em mam?o
Foltran, Dulcinéia Elizabete;Gon?alves, Paulo de Souza;Sabino, José Carlos;Igue, Toshio;Vilela, Regina Célia F.;
Bragantia , 1993, DOI: 10.1590/S0006-87051993000100002
Abstract: genetic and phenotypic parameters of papaya (carica papaya l.) were estimated for plant height, stem diameter at 10 and 50 cm height (above the soil surface), number of leaves, internodes length and foliar index at the ages of three, five and seven months old. the experiment was conducted using eight half-sib progenies at tietê experimental station, state of s?o paulo, brazil. the results of the variance analyses showed significant effects for plant height for the three ages; for stem diameter at 10 cm height, in seven month old plants; and for median internodes length in three and five month old plants. estimates for the genotypic determination coefficient, for the average of progenies, were high in most of the cases. these results showed the possibility of a successful mass selection of papaya, for the variables studied.
Caracteriza??o genética de Calophyllum brasiliense Camb. em duas popula??es de mata ciliar
Botrel, Maria Carolina Gaspar;Souza, Anderson Marcos de;Carvalho, Dulcinéia de;Pinto, Sheila Isabel do Carmo;Moura, Márcia Cristina de Oliveira;Estopa, Regiane Abjaud;
Revista árvore , 2006, DOI: 10.1590/S0100-67622006000500016
Abstract: calophyllum brasiliense camb. is a predominant species in areas with high water saturation such as the riparian forests in southern minas gerais state. the objective of this research was to study the genetic variability of c. brasiliense populations. twenty individuals were sampled from two populations located in funil dam in lavras-mg. isozyme eletrophoresis analysis provided evidence of 17 alleles distributed in 8 loci, which were represented in five enzymatic systems: a-esterase, b esterase, acid phosphatase, malate dehydrogenase and transaminase oxalacetatum glutamate. the diversity indexes showed a low number of alleles per loci in both populations, pop i (1.75) and pop ii (1.50). the polymorphic loci percentage (p) were 37.5% and 50% in populations i and ii respectively. the mean heterozygosities were 0.119 and 0.111, while the expected was 0.131 and 0.112. the number of migrants (nm) between populations was 2.70. the estimated effective size was 18 individuals for population i, and 19 for population ii.
Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests
Evania Galv?o Mendon?a,Anderson Marcos de Souza,Fábio de Almeida Vieira,Regiane Abjaud Estopa,Cristiane Aparecida Fioravante Reis,Dulcinéia de Carvalho
International Journal of Forestry Research , 2014, DOI: 10.1155/2014/305286
Abstract: The objective of this study was to assess the genetic variability in two natural populations of Calophyllum brasiliense located along two different rivers in the state of Minas Gerais, Brazil, using RAPD molecular markers. Eighty-two polymorphic fragments were amplified using 27 primers. The values obtained for Shannon index ( ) were 0.513 and 0.530 for the populations located on the margins of the Rio Grande and Rio das Mortes, respectively, demonstrating the high genetic diversity in the studied populations. Nei’s genetic diversity ( ) was 0.341 for the Rio Grande population and 0.357 for the Rio das Mortes population. These results were not significantly different between populations and suggest a large proportion of heterozygote individuals within both populations. AMOVA showed that 70.42% of the genetic variability is found within populations and 29.58% is found among populations ( ). The analysis of kinship coefficients detected the existence of family structures in both populations. Average kinship coefficients between neighboring individuals were 0.053 ( ) in Rio das Mortes and 0.040 ( ) in Rio Grande. This could be due to restricted pollen and seed dispersal and the history of anthropogenic disturbance in the area. These factors are likely to contribute to the relatedness observed among these genotypes. 1. Introduction Of all the ecosystems that constitute the Brazilian Semideciduous Forests, riparian forests contribute significantly to the conservation of biodiversity, mainly due to the relationship between ecological corridors and ecosystem functioning [1]. Because of their interconnectedness, riparian forests play a central role in biogeographical and evolutionary shifts as they facilitate seed dispersal [2]. In Brazil, even though riparian forests are protected by law in the Forest Code (Law 4.771 of 1965), these environments suffer the consequences of human activity, mainly resulting from fragmentation and degradation. The reduction of population sizes and the isolation of populations are direct consequences of anthropogenic activities, resulting in inbreeding and genetic drift [3, 4]. Understanding the distribution of genetic variability of tree populations in these areas is fundamental in management programs aimed at conservation and the survival of riparian forest species. There are a large number of tree species that occur in riparian forests and as such the choice of the target species for genetic and ecological studies is crucial. Among them, Calophyllum brasiliense Camb. (Clusiaceae), a Neotropical tree commonly known as “guanandi,”
Diversidade e estrutura genética espacial de Calophyllum brasiliense Camb. (Clusiaceae) em uma floresta paludosa
Reis, Cristiane Aparecida Fioravante;Souza, Anderson Marcos de;Mendon?a, Evania Galv?o;Gon?alvez, Flávio Rodrigues;Melo, Rodrigo Magno Guimar?es;Carvalho, Dulcinéia de;
Revista árvore , 2009, DOI: 10.1590/S0100-67622009000200008
Abstract: the areas of occurrence of swampy forests have been altered and restricted due to destruction and fragmentation processes, with consequent reduction of their population size. calophyllum brasiliense camb. is an abundant tree species in riparian environments for its preference for colonizing high water saturation soils, being considered specialist in habitat. besides the ecological consequences, such as mortality and low recruitment, genetic variability loss can occur, compromising the viability of the species in the place. thus, molecular markers are necessary to evaluate the genetic effects. within this context, a census of the species was conducted in a swampy forest fragment, with four height classes being established for genetic analysis. the alloenzymatic markers revealed a total of 11 polymorphic loci with a mean number of 2.0 alleles in each locus it was not observed the loss or alleles fixation. the observed heterozigosity was larger than that expected for classes ii and iv, evidencing heterozygote excess. classes i and iii presented homozygote excess, the value being non significant for class i. the genetic diversity values found were considered high compared to other tree species. thus, the occurrence of mutations and the incorporation of new alleles in the population are high, besides increasing the recombination number and multiple paternities in the progenies. overall, the analysis of the spatial distribution of the genotypes was random for most classes analyzed, except for class i, where the individuals close to a 10m distance presented family structure. the high genetic diversity of this species and absence of spatial structuring of the genotypes in most of the classes analyzed should be considered for conservation planning of this area.
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