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Search Results: 1 - 10 of 181671 matches for " Douglas Vinícius Bassalobre de Freitas "
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Construction of Vector for Functional Analysis of the Intrinsically Bent DNA in the ACE3 Replicator from Drosophila melanogaster  [PDF]
Douglas Vinícius Bassalobre de Freitas, Fabrícia Gimenes, Adriana Fiorini, Fabiana dos Santos Rando, Quirino Alves de Lima Neto, Maria Aparecida Fernandez
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41002

DNA replication is a crucial process for species survival, nevertheless it is not clear which factors define origin selection in multicellular eukaryotes. Developmental gene amplification systems, such as the one described during ovarian follicles development in Drosophila melanogaster, are useful tools for studying of DNA replication process in these organisms. We previously described that the well characterized third chromosome amplified domain of D. melanogater displays three intrinsically bent DNA sites: b1, localized at an amplification control element (ACE3), b2 and b3, both localized at the preferential origin ori-β. This proposal aimed to construct a Drosophila transformation vector, which contains a short deletion at the ACE3, in order to reduce the intrinsically bent DNA site b1, and analyze the functional role of this site in the gene amplification process. Through a series of cloning steps, we obtained a Big Parent vector derivative, containing a deletion at the positions 176-

Press?o arterial elevada e obesidade abdominal em adolescentes
Casonatto, Juliano;Ohara, David;Christofaro, Diego Giuliano D;Fernandes, R?mulo Araújo;Milanez, Vinícius;Dias, Douglas Fernando;Freitas Júnior, Ismael;Oliveira, Arli Ramos de;
Revista Paulista de Pediatria , 2011, DOI: 10.1590/S0103-05822011000400015
Abstract: objective: to analyze the association between abdominal obesity and high blood pressure among adolescents of public schools from londrina (pr), brazil. methods: the sample was composed by 656 adolescents with age ranging from ten to 13 years old. the following measures were taken: body mass, height, waist circumference and blood pressure at rest. mann-whitney and student's t-test were used to compare and analyze numerical variables. the chi-square test analyzed the association between blood pressure and waist circumference. results: association between abdominal obesity and high blood pressure was present in both genders (pr 2.7; 95%ci 1.8-4.2). abdominal obesity was associated with higher blood pressure independently of age. conclusions: abdominal obesity was associated to high blood pressure in adolescents.
Neoplasia papilar cística do pancreas
Pinto Junior, Francisco Edilson Leite;Moraes, Marcus Vinícius de;Oliveira, álisson Giovani Freitas de;
Revista do Colégio Brasileiro de Cirurgi?es , 2001, DOI: 10.1590/S0100-69912001000400015
Abstract: papyllary cystic tumor of the pancreas, so-called frantz's tumor, is rare. clinical presentation of this disease is usually a slowly growing abdominal mass with or without abdominal pain, affecting predominantly young females. its pathogenesis is still unknown . surgical resection is usually curative, and prognosis is excellent. the authors report two pancreatic tumor cases(frantz's tumor) in women aged 26 and 31 years old. pre operative assessment showed a solid-cystic tumor of the tail and body of the pancreas. an extended distal pancreatectomy was performed without splenic preservation.
Bilateral nongranulomatous anterior uveitis associated with chancre of the tongue: initial presentation of syphilis
Clovis Arcoverde de Freitas-Neto, Vinícius Monteiro Castro, Daniel Vitor Vasconcelos-Santos
Journal of Ophthalmic Inflammation and Infection , 2013, DOI: 10.1186/1869-5760-3-33
Abstract: Findings: A 51-year-old male presented with bilateral nongranulomatous anterior uveitis associated with an isolated syphilitic chancre of the tongue that was successfully treated with parenteral benzathine penicillin.Syphilis has the potential to lead to any type of intraocular inflammation. A careful review of the symptoms is useful for the detection of extraocular signs of syphilitic infection.
Polipose hiperplásica: relato de caso
Poswar, Fabiano de Oliveira;Carneiro, Jair Almeida;Monteiro, Vinícius Afonso;Freitas, Magno Otávio Salgado de;
Revista Brasileira de Coloproctologia , 2010, DOI: 10.1590/S0101-98802010000300010
Abstract: hyperplastic polyposis is a rare condition, characterized by the presence of multiple hiperplastic polyps in the colon. it is reported a case of a 29-year-old patient who presented hyperplastic polyposis associated with mixed polyps.
Contribui??o da imuno-histoquímica na avalia??o de fatores prognósticos e preditivos do cancer de mama e no diagnóstico de les?es mamárias
Salles, Marcio de Almeida;Cúrcio, Vinícius Silva;Perez, Amanda Arantes;Gomes, Douglas Soltau;Gobbi, Helenice;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2009, DOI: 10.1590/S1676-24442009000300006
Abstract: objective: to critically analyze the contribution of immunohistochemistry to the assessment of prognostic and predictive factors of breast cancer, to the search for micrometastases in sentinel lymph nodes, and to the differential diagnosis of mammary lesions. methods: an observational retrospective study was carried out reviewing all cases of mammary lesions submitted to immunohistochemistry at the breast pathology laboratory of federal university of minas gerais between 2001 and 2007. according to immunohistochemistry indication, the cases were classified into three categories: assessment of prognostic and predictive factors, search for epithelial metastatic cells in sentinel lymph nodes and differential diagnosis. results: immunohistochemistry was performed in 1,294 cases, with 4,101 reactions using 21 different antibodies. in most cases, immunohistochemistry was conducted to assess predictive/prognostic factors (1,106 cases, 85.5%), followed by search for epithelial metastatic cells in sentinel lymph nodes (134 cases, 10.6%) and differential diagnosis of breast lesions (51 cases, 3.9%). good quality reactions, with significant contribution to the immunohistochemistry study, were obtained in 1,247 cases (96.4%). in 47 cases (3.6%) the results were inconclusive due to fixation problems (autolysis) in the pre-analytical phase. conclusion: our data confirm that the immunohistochemical study is an important tool to the assessment of predictive and prognostic factors, search for micrometastases in sentinel lymph nodes, and differential diagnosis of mammary lesions. the widest use of immunohistochemistry was in the assessment of predictive and prognostic factors of breast cancer.
Composi??o química, vitreosidade e digestibilidade de diferentes híbridos de milho para suínos
Cantarelli, Vinícius de Souza;Fialho, Elias Tadeu;Sousa, Raimundo Vicente de;Freitas, Rilke Tadeu Fonseca de;Lima, José Augusto de Freitas;
Ciência e Agrotecnologia , 2007, DOI: 10.1590/S1413-70542007000300036
Abstract: this study was conducted in order to evaluate the chemical composition, vitreousity endosperm and nutritional values of different hybrids of corn through of chemical analysis and a metabolism assay with growing pigs. the metabolism assay were conducted by utilizing 24 crossbred (ld x.lw) barrows with 35,6kg+- 3,05kg keept in metabolism cages . the experimental treatments were formed by six (6) diets formulated with hybrids of corn: "corn oil", corn qpm, corn-dent, semi-dent, and two corns-flints. the values obtained vitreousity, digestibility coefficients of the crude protein, and energy digestible for corns high oil, qpm, dent, semi-dent, hard 1 and hard 2 were 78,5%; 71,7%; 57,2%; 68,2%; 75,9%; 82,8%; 80,14%; 82,35%; 69,71%; 76,98%; 75,61%; 3680, 3426; 3597; 3441; 3340 and 3469 kcal/kg, respectively. the data shown significant differences (p<0,005) for the values of vitreousity (p<0,005) among the corns studied. corns varieties with hard texture show a higher vitreousity as compared with those semi-dent and corn dent varieties. there were significant differences in digestibility coefficients of dry matter, digestibility coefficients of protein crude and energy digestible among the corn varieties studied (p<0,05). the "corn oil" and the corn dent varieties shown higher digestibility values than others. the nitrogen retention showed significant differences (p<0,05) among the corns studied. the corns varieties dent, qpm and "corn oil" showed higher digestibility as compared with the semi-dent and corns-flints varieties. in conclusion the corn-dent, "corn oil"and corn qpm varieties showed better nutritional values than others varieties studied.
Abundance of Conepatus chinga (Carnivora, Mephitidae) and other medium-sized mammals in grasslands of southern Brazil
Kasper, Carlos B.;Bastazini, Vinícius A. G.;Soares, José B. G.;Freitas, Thales R. O. de;
Iheringia. Série Zoologia , 2012, DOI: 10.1590/S0073-47212012000300009
Abstract: between january 2007 and december 2010, the abundance of medium-sized mammals was studied, with special focus on the molina's hog-nosed skunk, conepatus chinga (molina, 1782), at four locations in southern brazil. in this study, transect line methodology was used to obtain data for distance analyses. transects were traveled by car at night, searching with spotlights along the edges of secondary roads in agricultural landscapes. along 1,811 km, we obtained 620 observations of 20 mammal species. the most common species was the exotic european hare, lepus europaeus (pallas, 1778); the highest abundance estimated for south america was observed in one of the study areas, where its density was estimated as 32 individuals/km2. carnivores were the most commonly recorded mammals, represented by 10 species and comprising 51% of all observations. molina's hog-nosed skunk occurred in all study areas, but occurred in sufficient numbers to obtain density estimates in only two of the areas. we estimated 1.4 to 3.8 individuals/km2, in the first density estimate made by the transect method for a member of conepatus in the neotropics. these values are similar to those estimated for north american species of mephitidae. in brazil, c. chinga is apparently more abundant in the pampa biome than in the grasslands of the atlantic forest. for two other carnivores, lycalopex gymnocercus (fisher, 1814) and cerdocyon thous (linnaeus, 1766), we estimated preliminary densities that were similar to those previously cited for different regions.
Brazilian kefir: structure, microbial communities and chemical composition
Magalh?es, Karina Teixeira;Pereira, Gilberto Vinícius de Melo;Campos, Cássia Roberta;Dragone, Giuliano;Schwan, Rosane Freitas;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000200034
Abstract: microbial ecology and chemical composition of brazilian kefir beverage was performed. the microorganisms associated with brazilian kefir were investigated using a combination of phenotypic and genotypic methods. a total of 359 microbial isolates were identified. lactic acid bacteria (60.5%) were the major isolated group identified, followed by yeasts (30.6%) and acetic acid bacteria (8.9%). lactobacillus paracasei (89 isolates), lactobacillus parabuchneri (41 isolates), lactobacillus casei (32 isolates), lactobacillus kefiri (31 isolates), lactococcus lactis (24 isolates), acetobacter lovaniensis (32 isolates), kluyveromyces lactis (31 isolates), kazachstania aerobia (23 isolates), saccharomyces cerevisiae (41 isolates) and lachancea meyersii (15 isolates) were the microbial species isolated. scanning electron microscopy showed that the microbiota was dominated by bacilli (short and curved long) cells growing in close association with lemon-shaped yeasts cells. during the 24 h of fermentation, the protein content increased, while lactose and fat content decreased. the concentration of lactic acid ranged from 1.4 to 17.4 mg/ml, and that of acetic acid increased from 2.1 to 2.73 mg/ml. the production of ethanol was limited, reaching a final mean value of 0.5 mg/ml.
Características da carca?a e viabilidade econ?mica do uso de cloridrato de ractopamina para suínos em termina??o com alimenta??o à vontade ou restrita
Cantarelli, Vinícius de Souza;Fialho, Elias Tadeu;Almeida, Erin Caperuto de;Zangeronimo, Márcio Gilberto;Amaral, Níkolas de Oliveira;Lima, José Augusto de Freitas;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000300032
Abstract: the objective of this research was to evaluate the supplementation of 5ppm of ractopamine (rac), associated or not with feed restriction, on carcass characteristics on finishing pigs and the economic viability of its use. thirty hybrid barrows (life weight of 107.2±6.2kg), receiving the experimental diets during 28 days before the slaughter. a randomized blocks design in factorial scheme 2 x 2 + 1 (with or without rac in diets with 1.04% of total lysine; ad libitum or feed restriction of 15% and an additional treatment - control - ad libitum, without rac containing 0.8% of lysine - standard diet), totalizing five treatments and six replicates. the addiction of rac per se increased the loin eye area, the meat percentage, the carcass meat:fat ratio, the allowance index, the gross earnings and the production net earnings and decreased the animals backfat thickness. when associated with feed restriction, the rac decreased the carcass fat percentage. compared to standard diet, the rac increased the carcass yield, the carcass meat percentage and the allowance index paid to the producer and decreased the backfat thickness. the rac associated at the feed restriction decreased the fat carcass percentage and increased the carcass meat:fat ratio and the gross earnings. thus, the supplementation with 5ppm of rac, associated or no at the feed restriction, improves the carcass characteristics and the economic viability of production.
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