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Search Results: 1 - 10 of 5904 matches for " Dora Flores "
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DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence  [PDF]
Carlos Castro, Alejandro Hernandez, Luis Alvarado, Dora Flores
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.61011
Abstract: Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the trnH-psbA sequence. The ITS sequence presented the highest variation rates, while the phylogeny constructed with the matK sequence obtained the highest percentage of solved monophyletic groups. Through Pearson’s correlation analysis, it was possible to determine the existence of a significant correlation between the ITS region and psbA-trnH, and the matK and psbA-trnH sequences, but not between ITS and matK. The phylogenies constructed with the ITS + matK barcodes and ITS + matK + psbA-trnH presented the highest percentage for resolution. However, considering the cost efficiency and the facilitated recovery by using PCR, the matK + ITS combination is recommended.
Propagación por estacas y estudio preliminar del establecimiento in vitro de granadilla (Passiflora ligularis, juss)
Dora Flores,Jaime Brenes,Ana Guzmán
Tecnología en Marcha , 2010,
Abstract: La granadilla (Passiflora ligularis) es una especie de polinización abierta, razón por la cual presenta un alto porcentaje de variabilidad genética. En Costa Rica este cultivo ofrece una opción de diversificación agrícola para la zona de Los Santos y El Guarco de Cartago. El uso de técnicas biotecnológicas como el cultivo de tejidos favorece la explotación de este, ya que permitiría el establecimiento de plantaciones más homogéneas que contribuirían a mejorar el rendimiento y la calidad del fruto. El objetivo de esta investigación fue establecer la metodología de propagación por estacas y realizar un estudio preliminar que conduzca al establecimiento in vitro de material. Se realizaron tres introducciones de estacas al invernadero y es empleado Agrirrot (0,01% AIB) para inducir el enraizamiento; se obtuvo un 50% o más de enraizamiento y brotación de las estacas en cada introducción. Para el establecimiento in vitro se introdujeron brotes provenientes de estacas pretratadas en el invernadero. Se obtuvo un 37,5% de explantes muertos por quema, un 50% presentó contaminación fungosa, bacteriana o ambas y un 12,5% de material limpio y brotado.
Establecimiento in vitro y pruebas preliminares de micropropagación en medio semisólido y líquido de frambuesa (Rubus idaeus L.)
Fiorella Jones Castro,Dora Flores Mora
Tecnología en Marcha , 2007,
Abstract: La propagación de la frambuesa se realiza tradicionalmente de forma vegetativa por separación de corona o brote etiolado; sin embargo, en vista de la demanda masiva de material sano y vigoroso, se ha generado un gran interés por el uso de la técnica de cultivo de tejidos. Para el establecimiento in vitro de la frambuesa, se realizaron tres tratamientos, la mayor supervivencia (45%) se obtuvo con una desinfección superficial de 6gL-1 Agri-mycin y Bisolex y 5gL-1 Ferbam durante 60 min, seguida de una exposición a CaClO2 3,5% por 15 min en bomba al vacío. Se probaron dos sistemas para la micropropagación, el medio semisólido y el medio líquido en inmersión temporal, utilizando el medio de cultivo M&S complementado con BAP, AG3 y ácido ascórbico, con un pH de 5,5. Los resultados obtenidos para ambos sistemas fueron estadísticamente similares. Los dos presentaron buena brotación y crecimiento; sin embargo las vitroplantas en inmersión temporal se observaron más vigorosas.
Effect of Using Two Different Types of Carbon Nanotubes for Blackberry (Rubus adenotrichos) in Vitro Plant Rooting, Growth and Histology  [PDF]
Dora Flores, Randall Chacón, Luis Alvarado, Alexander Schmidt, Carlos Alvarado, Juan Chaves
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.524367
Abstract: Nanoparticles are able to interact with biomolecules, creating functional nanosystems for transportation within in vivo cells, and leading to the study of their potential applications in the field of plant biotechnology. Therefore, the aim of this research was to determine the growth and rooting effect of functionalized (SWCNTs-COOH) and non-functionalized nanoparticles with iron residue inner particles (SWCNTs-Fe) in blackberry (Rubus adenotrichos) in vitro plants. Two types of SWCNTs were used, both of them characterized in a solid sample through Raman spectroscopy (λ = 532 nm) showing differences in the G band between SWCNT + Fe and SWCNT + COOH. The in vitro plants (approximately 15 mm length) were inoculated in a rooting medium. Six treatments were established: 4, 8, 12 μg/ml for each type of SWCNTs and a control without nanotubes. The assessed variables consisted of the average number of days for root emergence, average number of roots per plant, average root length per plant and the average stem length. This study determined that, in general, the SWCNTs-COOH promoted the growth of the in vitro plants under this assay, when compared to the SWCNTs-Fe trials. The lowest SWCNTs-COOH dose evidenced the best results for the assessed variables. Additionally, the histological analysis also evidenced that the plants treated with SWCNTs-COOH nanotubes (4 μg/ml) increased their cellular metabolism when compared to the control group.
Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
Allan Meneses,Dora Flores,Miguel Mu?oz,Griselda Arrieta
Revista de Biología Tropical , 2005,
Abstract: With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of the gelling agent PhytagelTM (1.8, 2.4, and 3 gL-1) and four 2,4-D concentrations (2.26, 4.52, 6.78, and 9.05 mu M) on the induction and subsequent regeneration of embryogenic calli. On the second experiment, the pre-regeneration phase was modified; calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. In embryogenesis induction, 35% calligenesis was obtained using the MS culture medium supplemented with 6.78 mu M of 2,4-D and 2.4 gL-1 PhytagelTM , whereas on the control treatment (MS medium supplemented with 9.05 mu M of 2,4-D and 3 gL-1 PhytagelTM ) 24% calligenesis was obtained. In addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). Furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase. Rev. Biol. Trop. 53(3-4): 361-368. Epub 2005 Oct 3.
New Actors on Stage: Analysis of the Emergent Forms of Collective Action in the European Context  [PDF]
Dora Fonseca
Open Journal of Social Sciences (JSS) , 2014, DOI: 10.4236/jss.2014.21005
Abstract: In this article our focus will be on civil society’s responses triggered by the imposition of the societies of austerity. The analysis will be centred on an emerging collective actor, the indignados, and on the conditions that fostered its formation and processes involved in the construction of its identity. To accomplish this task, we retrieved Ernesto Laclau’s political logic of populism and the concept of political developed by Chantal Mouffe and applied them to the formation of the indignados’ identity. This process is conceived in terms of the construction of antagonistic frontiers which divide the social into two opposing fields. This conception allows us to develop an analysis based on the notion of social conflict, as well as a reflection on the potentialities embodied by this new social actor, despite their presentation by the dominant discourse as utopic and therefore impossible. In this proposal, it is our aim to provide a better understanding of what is at stake when we talk about the indignados and a clearer perception of the political dimension of both struggle and resistance.
Efecto de diferentes tipos de propagación en el rendimiento de mora Vino (Rubus adenotrichus)
Orozco-Rodríguez,Rafael; Flores-Mora,Dora; Argüello-Delgado,Félix;
Agronomía Mesoamericana , 2011,
Abstract: effect of various types of propagation on the yield of wine blackberries (rubus adenotrichus). this research project was intended to determine the effect of two different systems of propagation-macropropagation and micropropagation- on yield of two varieties of blackberries (rubus adenotrichus): "red thorn- wine" and "thornless wine." this experiment was carried out in 2005 at la luchita, in el guarco, cartago, costa rica. the harvest took place during the years 2007 and 2008. significant differences were found (p=0,0034) between the systems for propagation of the "red-thorn wine" variety, in 2007. the plants resulting from macropropagation showed the higher production average. the production for the "thornless wine" variety was null that year. in 2008 there were no significant differences found (p>0,6207) between the two propagation systems for either one of the varieties. when compared, "red-thorn wine" variety showed a higher production under the two propagation systems (p<0,00183).
Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
Meneses,Allan; Flores,Dora; Mu?oz,Miguel; Arrieta,Griselda; Espinoza,Ana M;
Revista de Biología Tropical , 2005,
Abstract: with the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety cr-5272 (oryza sativa l.), two independent experiments were performed. the first experiment consisted in the effect of combination of three concentrations of the gelling agent phytageltm (1.8, 2.4, and 3 gl-1) and four 2,4-d concentrations (2.26, 4.52, 6.78, and 9.05 m m) on the induction and subsequent regeneration of embryogenic calli. on the second experiment, the pre-regeneration phase was modified; calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. in embryogenesis induction, 35% calligenesis was obtained using the ms culture medium supplemented with 6.78 m m of 2,4-d and 2.4 gl-1 phytageltm , whereas on the control treatment (ms medium supplemented with 9.05 m m of 2,4-d and 3 gl-1 phytageltm ) 24% calligenesis was obtained. in addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase. rev. biol. trop. 53(3-4): 361-368. epub 2005 oct 3.
Enraizamiento de mora (Rubus adenotrichus) en medio líquido en el sistema de inmersión temporal y su aclimatación en invernadero
Dora Flores Mora,Randall Chacón Cerdas,Vilma Jiménez Bonilla,Fabián Ortiz Sancho
Tecnología en Marcha , 2012,
Abstract: Se presentan los resultados de una investigación cuyo objetivo fue evaluar el efecto de tres concentraciones de ácido Indolbutírico (AIB) sobre el enraizamiento in vitro de la mora “vino sin espinas”. Se utilizó un sistema de inmersión temporal automatizado para determinar la sobrevivencia de las plántulas durante la aclimatación. Se empleó un medio de cultivo líquido MS con sales al 50%, sacarosa al 3% y pH 5.7, suplementado con 0.125 (T1), 0.250 (T2) y 0.500 (T3=control) mg/L de AIB. En cada recipiente para inmersión temporal se colocaron 200ml de medio y 15 explantes, se programó la inmersión por 5 minutos cada 12 horas, se incubaron a 20°C y 16h luz por ocho semanas. Se aplicó el análisis de ANDEVA y las pruebas de Tukey para evaluar la longitud del tallo, la longitud de las raíces y el número de entrenudos. Las vitroplantas se aclimataron en un sustrato compuesto de suelo, carbón vegetal y granza de arroz (3:1:1) y se determinó el porcentaje de sobrevivencia durante cuatro semanas. T1 registró la mayor longitud del tallo (6.49 cm) y fue estadísticamente diferente a T2 y T3. T3 presentó la mayor longitud radical (4.73 cm) y fue estadísticamente igual a T1 y T2. Ningún tratamiento mostró diferencia estadística en el número de entrenudos (8 promedio). T2 mostró la mayor sobrevivencia de plántulas a la cuarta semana de aclimatación con un 78.6% seguido del T3 (57.9%) y T1 (43.3%). Se concluyó que las plantas provenientes de T2 (0.250 mg/L AIB), las cuales presentaron una longitud promedio de tallo de aproximadamente 6 cm y una longitud promedio de raíz de aproximadamente 4cm, mostraron la mayor sobrevivencia durante la etapa de aclimatación. La investigación se realizó en el Tecnológico de Costa Rica durante el 2008.
CULTIVO DE TEJIDOS EN Ficus carica CON MINIESTACAS
Dora María Flores-Mora,Vilma Jiménez-Bonilla,Randall Chacón-Cerdas
Agronomía Mesoamericana , 2009,
Abstract: Este ensayo se llevó a cabo en el Centro de Investigación en Biotecnología, del Instituto Tecnológico de Costa Rica, Cartago, Costa Rica, entre los a os 2006-2008. Tuvo como objetivo evaluar el cultivo in vitro del higo. Para el establecimiento del material vegetal in vitro se utilizaron miniestacas de 3 cm, de longitud provenientes de material propagado vegetativamente. Para su desinfección se lavaron y se sumergieron en una solución de 6 g/l de benomilo (50 % i.a.), 6 g/l de sulfato de gentamicina (2 % i.a.) y de clorhidrato de oxitetraciclina (6 % i.a.) y 3,5 g/l de dimetiltiocarbamato (76 % i.a.) durante 90 minutos. Luego se realizó una segunda desinfección con hipoclorito de calcio al 3,5 % p/v durante 10 minutos. Se inocularon en un medio M&S (1962) completo, más 1mg/l de BAP, 300 mg/l de acido ascórbico y 175 mg/l de ciprofloxacina. En la etapa de multiplicación se separaron los brotes desarrollados, se inocularon y se empleó como medio base M&S (1962) completo, suplementado con 1,0; 0,5 y 0 mg/l de BAP. Para la etapa de enraizamiento, se implementaron dos tratamie ntos, M&S (1962) completo con 0,5 y 0 mg/l de AI B. Se obtuvo un 31,67 % de supervivencia y asepsia del material en la etapa de introducción y un promedio de tres brotes por explante al mes de incubación. El mayor promedio de brotes por explante se logró en el tratamiento con 1,0 mg/l de BAP y el mayor porcentaje de enraizamiento se obtuvo en el tratamiento sin regulador. Durante la fase de aclimatación, se logró un 100 % de supervivencia.
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