oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 152 )

2018 ( 214 )

2017 ( 238 )

2016 ( 353 )

Custom range...

Search Results: 1 - 10 of 213168 matches for " Dianelys Qui?ones Pérez "
All listed articles are free for downloading (OA Articles)
Page 1 /213168
Display every page Item
Evaluación del medio cromogénico CromoCen? ENT para el diagnóstico clínico de Enterococcus
Díaz Pérez,Marilyn; Iglesias Torrens,Yaidelys; Zhurbenko,Raisa; Quiones Pérez,Dianelys;
Revista Cubana de Medicina Tropical , 2012,
Abstract: introduction: the frequent incidence of enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means. objective: to broaden the evaluation of functionality of cromocen? ent chromogenic medium for the isolation and identification of enterococcus spp. from clinical samples. methods: one hundred and fifty clinical samples were analyzed (urine, blood, feces, vaginal smears, skin lesion exudates and exudates from catheters) in the january-april period, 2010 by using the chromogenic medium and the corresponding conventional culture media as controls; the incidence of enterococcus spp was evaluated. the isolations were identified with 12 biochemical tests. from the biochemical identification data, it was possible to determine the quality indicators for both cromocen? ent and the reference media. results: the chromogenic medium encouraged the growth of enterococcus species in 24 hours, allowing their easy recognition due to the pink coloration of the colonies. the diagnostic quality indicator values were over 95 %. the highest percentage of isolates was observed in the urine samples. enterococcus faecalis was the mostly found species. conclusions: cromocen? ent allowed quick and accurate identification of enterococcus spp. from various clinical samples.
Evaluación del medio cromogénico CromoCen ENT para el diagnóstico clínico de Enterococcus Assessment of the CromoCen ENT chromogenic medium for clinical diagnosis of Enterococcus species
Marilyn Díaz Pérez,Yaidelys Iglesias Torrens,Raisa Zhurbenko,Dianelys Quiones Pérez
Revista Cubana de Medicina Tropical , 2012,
Abstract: Introducción: la frecuente incidencia de Enterococcus en los hospitales y su creciente resistencia antimicrobiana a nivel mundial, ha incrementado la necesidad de su vigilancia y control intrahospitalario, por lo que resulta imprescindible contar con medios diagnósticos más sensibles y exactos. Objetivo: ampliar la evaluación de la funcionalidad del medio cromogénico CromoCen ENT para el aislamiento e identificación de Enterococcus spp. procedentes de muestras clínicas. Métodos: se analizaron 150 muestras clínicas (orina, sangre, fecales, exudados vaginales, exudados de lesiones de piel y de catéteres) desde enero hasta abril de 2010, empleando el medio cromogénico y los medios convencionales correspondientes como controles, se evaluó la incidencia de Enterococcus spp. Se identificaron los aislamientos con un conjunto de 12 pruebas bioquímicas. A partir de los datos de la identificación bioquímica se determinaron los indicadores de calidad tanto para el medio CromoCen ENT como para los medios de referencia. Resultados: el medio cromogénico promovió el crecimiento de Enterococcus spp. en solo 24 h, lo cual permitió su fácil reconocimiento por la coloración rosada de las colonias. Los indicadores de calidad diagnóstico mostraron valores superiores a 95 %. El mayor porcentaje de aislamientos se obtuvo en las muestras de orina. Enterococcus faecalis resultó la especie mayormente encontrada en el total de las muestras. Conclusiones: CromoCen ENT permitió la correcta y rápida identificación de Enterococcus spp. procedentes de diversas muestras clínicas. Introduction: the frequent incidence of Enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means. Objective: to broaden the evaluation of functionality of CromoCen ENT chromogenic medium for the isolation and identification of Enterococcus spp. from clinical samples. Methods: one hundred and fifty clinical samples were analyzed (urine, blood, feces, vaginal smears, skin lesion exudates and exudates from catheters) in the January-April period, 2010 by using the chromogenic medium and the corresponding conventional culture media as controls; the incidence of Enterococcus spp was evaluated. The isolations were identified with 12 biochemical tests. From the biochemical identification data, it was possible to determine the quality indicators for both CromoCen ENT and the reference media. Results: the chromogenic medium encouraged the grow
Susceptibilidad antimicrobiana y factores de virulencia en especies de Enterococcus causantes de infecciones pediátricas en Cuba Antimicrobial susceptibility and virulence factors found in Enterococcus species causing pediatric infections in Cuba
Dianelys Quiones Pérez,Deisy Marrero,Bárbara Falero,Isis Tamargo
Revista Cubana de Medicina Tropical , 2008,
Abstract: Objetivos: conocer la prevalencia de especies de enterococos causantes de infecciones en ni o, su virulencia, susceptibilidad antimicrobiana e infecciones más frecuentes. Métodos: se estudiaron 156 aislamientos de enterococos causantes de infecciones en ni os ingresados en el Hospital "Octavio de la Concepción y La Pedraja", Holguín. Se llevó a cabo la identificación de especies y detección de factores de virulencia por métodos fenotípicos. Se determinó la concentración inhibitoria mínima para vancomicina, ciprofloxacina, ampicilina, gentamicina, estreptomicina, cloranfenicol, nitrofurantoína, eritromicina, tetraciclina, levofloxacina y norfloxacina, según las normas del Clinical Laboratory Standard Institute. Resultados: las cepas se aislaron de sangre, orina, heridas quirúrgicas, piel y tejido blando, oído medio, vulva, punta de catéter, líquido peritoneal, vagina, esperma, líquido cefalorraquídeo, y otras fuentes. La especie prevalente fue Enterococcus faecalis, seguido por Enterococcus faecium, Enterococcus gallinarum y Enterococcus raffinossus, Enterococcus casseliflavus y Enterococcus avium. La producción de proteasa y hemolisina se detectó solo en E. faecalis. E. faecium mostró porcentajes más altos de resistencia a ampicilina, ciprofloxacina, norfloxacina, levofloxacina, mientras que E. faecalis mostró más resistencia a eritromicina, cloranfenicol y tetraciclina. De E. faecalis 58 aislamientos y 12 de E. faecium presentaron altos niveles de resistencia a gentamicina, así como 45 y 32,3 % mostraron altos niveles de resistencia para estreptomicina, respectivamente. Se detectó una cepa de E. faecium resistente a vancomicina. Conclusiones: la circulación de cepas de enterococos virulentas y resistentes a diferentes antibióticos indica la necesidad del control de la infección enterocócica y la importancia del monitoreo de la susceptibilidad antimicrobiana, para prevenir la selección de cepas con estos rasgos dentro de las unidades hospitalarias y su posible expansión a la comunidad. Objectives: to find out the prevalence of Enterococcus species causing child infections, their virulence, antimicrobial susceptibility and most frequent infections. Methods: One hundred and fifty six isolations from Enterococcus causing infections in children, admitted to "Octavio de la Concepción y La Pedraja" hospital in Holguin, were studied. The phenotypical methods allowed identifying species and detecting virulence factors. Following Clinical Laboratory Standard Institute requirements, minimal inhibitory concentration was determined for vancomycin, ciprofloxacin, amp
Tratamiento de la infección por Helicobacter pylori: Comentario al respecto
Padrón Pérez,Noel; Fernández Vallín-Cárdenas,Eulalia; Quiones Pérez,Dianelys;
Revista Cubana de Investigaciones Biom??dicas , 1999,
Abstract: a bibliographic review on the schedules of treatment recommended in the infection caused by helicobacter pylori was made. the high rates of erradication attained by the triple and quadruple therapies allow their use as first and second line treatment, respectively. those affections associated with the infection, in which the erradicating treatment should be applied, are related. the utilization of antimicrobials in areas where the resistance was high reduced the efficacy of the therapy used. the anti-h. pylori vaccine became a new option of treatment. the use of different mucous sites of vaccination with adjuvants of low toxicity was also dealt with in this paper. the therapeutic effectiveness of the vaccine according to the authors proved to be high in experimental models and achieved 100 % of protection against the infection caused by this gramnegative bacterium.
Tratamiento de la infección por Helicobacter pylori: Comentario al respecto
Noel Padrón Pérez,Eulalia Fernández Vallín-Cárdenas,Dianelys Quiones Pérez
Revista Cubana de Investigaciones Biom??dicas , 1999,
Abstract: Se realizó una revisión bibliográfica sobre las pautas de tratamiento recomendadas en la infección causada por Helicobacter pylori. Las altas tasas de erradicación logradas por los esquemas triple y cuádruple, hacen que éstos sean utilizados como primera y segunda línea de tratamiento respectivamente. Se relacionan las afecciones asociadas con la infección, en las que se debe utilizar el tratamiento erradicador. El uso de antimicrobianos, en zonas donde la resistencia era elevada, redujo la eficacia del esquema utilizado. La vacuna anti-H. pylori constituyó una novedosa opción de tratamiento. La utilización de diferentes sitios mucosos de vacunación con adyuvantes de baja toxicidad, también se trataron en este trabajo. La efectividad terapéutica de la vacuna, según los autores, resultó alta en modelos experimentales y alcanzó 100 % de protección frente a la infección por esta bacteria gramnegativa. A bibliographic review on the schedules of treatment recommended in the infection caused by Helicobacter pylori was made. The high rates of erradication attained by the triple and quadruple therapies allow their use as first and second line treatment, respectively. Those affections associated with the infection, in which the erradicating treatment should be applied, are related. The utilization of antimicrobials in areas where the resistance was high reduced the efficacy of the therapy used. The anti-H. pylori vaccine became a new option of treatment. The use of different mucous sites of vaccination with adjuvants of low toxicity was also dealt with in this paper. The therapeutic effectiveness of the vaccine according to the authors proved to be high in experimental models and achieved 100 % of protection against the infection caused by this gramnegative bacterium.
Susceptibilidad antimicrobiana y factores de virulencia en especies de Enterococcus causantes de infecciones pediátricas en Cuba
Quiones Pérez,Dianelys; Marrero,Deisy; Falero,Bárbara; Tamargo,Isis; Llop,Alina; Kobayashi,Nobumichi; Del Campo,Rosa;
Revista Cubana de Medicina Tropical , 2008,
Abstract: objectives: to find out the prevalence of enterococcus species causing child infections, their virulence, antimicrobial susceptibility and most frequent infections. methods: one hundred and fifty six isolations from enterococcus causing infections in children, admitted to "octavio de la concepción y la pedraja" hospital in holguin, were studied. the phenotypical methods allowed identifying species and detecting virulence factors. following clinical laboratory standard institute requirements, minimal inhibitory concentration was determined for vancomycin, ciprofloxacin, ampicilline, gentamycin, streptomycin, chloranphenicol, nitrofurantoine, erythromycin, tetracycline, levofloxacin and norfloxacine. results: strains were isolated from the blood, the urine, surgical wounds, the skin and the soft tissue, the middle ear, the vulva, catheter point, peritoneal fluid, vagina, sperm, cerebrospinal fluid, and from other sources. the prevailing species was enterococcus faecalis followed by enterococcus faecium, enterococcus gallinarum y enterococcus raffinossus, enterococcus casseliflavus y enterococcus avium. protease and hemolysin was only detected in e. faecalis. e. faecium accounted for the highest percentage of resistance to ampicillin, ciprofloxacine, norfloxacin, levofloxacin whereas e. faecalis was more resistant to erythromycin, chloranphenicol and tetracycline. fifty eight e. faecalis isolates and 12 e. faecium isolates exhibited the highest levels of resistance to gentamycin, as well as 45 % and 32.3 % of samples had high levels of resistance to streptomycin respectively. a vancomycin-resistent e. faecium strain was detected. conclusions: the circulation of virulent and multiple resistant enterococcus strains indicates that enterococcus infection need to be controlled and the importance of monitoring antimicrobial susceptibility, in order to prevent the selection of strains with these characteristics in hospitals and their possible extension to the community .
Susceptibilidad antimicrobiana y bases genéticas de la resistencia de cepas de Enterococcus causantes de infecciones en Cuba
Quiones Pérez,Dianelys; Abreu Capote,Miriam; Marrero,Deisy; Alvarez,Ana Bertha; Ortiz,Cecilia; Salomé,Francisco; Llop,Alina; Campo,Rosa del;
Revista Panamericana de Salud Pública , 2011, DOI: 10.1590/S1020-49892011001200009
Abstract: objective: to identify infection-causing enterococcus species in cuban hospitals and determine their susceptibility to antimicrobial drugs, as well as their resistance mechanisms. methods: a total of 687 enterococcus isolates from 30 cuban hospitals in nine provinces of the country were studied over the period 2000-2009. the species were identified using both the conventional method and the automatic api? system. the minimum inhibitory concentration was determined for 13 antimicrobial drugs following the standards recommended by the clinical laboratory and standards institute. the polymerase chain reaction technique was used to characterize the genes that were resistant to aminoglycosides, erythromycin, tetracycline, and glucopeptides. the presence of beta-lactamase was determined by the chromogenic cephalosporin test. results: the most prevalent species were enterococcus faecalis (82.9%) and e. faecium (12.2%). resistance to glucopeptides (1.0%) was mediated by the vana and vanb genes. the strains resistant to ampicillin (6%) did not produce beta-lactamases. a high percentage of resistance to aminoglycosides was observed. gentamicin (31.0%) and streptomycin and amikacin (29.1%) were mediated by the aac(6')ie-aph(2")ia, aph(3')-iiia, ant(6)ia, and ant(3")(9) genes. a correlation was found between resistance to tetracycline (56.0%) and presence of the tet(m) (75.1%) and tet(l) genes (7.0%), while resistance to erythromycin (34.1%) was due to the erm(b) gene (70.9%). conclusions: resistance to vancomycin is infrequent in cuba, as opposed to a high level of resistance to aminoglycosides, which may be indicative of treatment failures. the microbiology laboratory is a cornerstone of enterococcus infection surveillance, along with ongoing monitoring of the susceptibility of these infections to antimicrobial drugs at a time when resistance of this microorganism is on the rise.
La caries dental como urgencia y su asociación con algunos factores de riesgo en ni?os
Pérez Quiones,José Alberto; Mayor Hernández,Félix; Pérez Padrón,Alejandro;
Revista M??dica Electr?3nica , 2010,
Abstract: the disorders produced by dental caries and other oral affections are multiple, making the patient assist to the urgency service. the purpose of this work was evaluating the relations among the sreptococcus mutans and lactobacillus and some demographic variables with dental caries in children, registered in five medical consulting rooms of contreras area, municipality and province of matanzas, for which we carried out an analytic study of the cases kind and controls. the sample was obtained by means of a polistaged, stratified, probabilistic design, and was formed by 900 6-12 years-old children (300 cases and 600 controls). there was a predominance of 6- 8 years-old children both in the cases group and in the control group, and there were not significant differences between the groups. there was prevalence of female children in controls and of male in cases. children from the cases group had a high infection level by streptococcus mutans and lactobacillus, and there was stated a positive association of dental caries with these variables. we designed an educative strategy to evaluate and increase the information level studied children had on this disease.
La caries dental como urgencia y su asociación con algunos factores de riesgo en ni os Dental caries as urgency and its association with some risk facts in children
José Alberto Pérez Quiones,Félix Mayor Hernández,Alejandro Pérez Padrón
Revista M??dica Electr?3nica , 2010,
Abstract: Las dolencias producidas por la caries dental y otras afecciones bucales son múltiples, lo que hace al paciente acudir a los servicios de urgencia. El propósito de este trabajo fue evaluar la relación del estreptococo mutans y lactobacilo y algunas variables demográficas con la caries dental en ni os, pertenecientes a cinco consultorios del área de Contreras, del municipio de Matanzas, para lo cual se realizó un estudio analítico del tipo de casos y controles. La muestra se obtuvo mediante un dise o muestral probabilístico estratificado polietápico y quedó conformada por 900 ni os (300 casos y 600 controles) de 6 a 12 a os de edad. Hubo un predominio de ni os en las edades de 6 a 8 a os, tanto en los casos como en el grupo control, y no se observaron diferencias significativas entre ambos grupos. El sexo femenino prevaleció en los controles, y el masculino, en los casos. Los ni os pertenecientes al grupo de casos, tenían un alto grado de infección por estreptococo mutans y lactobacilos, y se demostró asociación positiva de la caries dental con estas variables. Se dise ó una estrategia educativa para evaluar y elevar el nivel de información que sobre esta enfermedad tenían los ni os objetosde estudio. The disorders produced by dental caries and other oral affections are multiple, making the patient assist to the urgency service. The purpose of this work was evaluating the relations among the sreptococcus mutans and lactobacillus and some demographic variables with dental caries in children, registered in five medical consulting rooms of Contreras area, municipality and province of Matanzas, for which we carried out an analytic study of the cases kind and controls. The sample was obtained by means of a polistaged, stratified, probabilistic design, and was formed by 900 6-12 years-old children (300 cases and 600 controls). There was a predominance of 6- 8 years-old children both in the cases group and in the control group, and there were not significant differences between the groups. There was prevalence of female children in controls and of male in cases. Children from the cases group had a high infection level by streptococcus mutans and lactobacillus, and there was stated a positive association of dental caries with these variables. We designed an educative strategy to evaluate and increase the information level studied children had on this disease.
Asociación del Estreptococos mutans y lactobacilos con la caries dental en ni?os
Pérez Quiones,José Alberto; Duque de Estrada Riverón,Johany; Hidalgo Gato- Fuentes,Iliana;
Revista Cubana de Estomatolog?-a , 2007,
Abstract: dental caries is a disease widely spread in the world, therefore, it has been and it is still the most frequent disease of modern man. due to the high incidence of this oral disease, and to the great need to prevent it, it was decided to conduct this investigation in order to evaluate the connection of the cariogenic microorganisms and some demographic variables with dental caries in children. a case-control analytical study was undertaken in the municipality of matanzas, province of matanzas, during the academic course 2004-2005. the study group was composed of 11 311 children aged 6-12. a randomized sample of 900 children (300 cases and 600 controls) was selected. the highest percentage of white children (62 %) were affected by caries, 91.3 % and 40 % of the children from the case group had an elevated degree of infection caused by streptococcus mutans y lactobacilli, respectively, which showed a positive association between dental caries and these variables.
Page 1 /213168
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.