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Search Results: 1 - 10 of 309035 matches for " Diana J.; Guerrero-Castillo "
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Detección del Polimorfismo 1843 en el Gen Receptor de Ryanodina Mediante la Técnica de PCR-SSCP
Castro-Molina,Susan L.; Ariza-Botero,Manuel F.; Ríos-Rodriguez,Marcela; Moreno,Diana J.; Guerrero-Castillo,Germán H;
ORINOQUIA , 2011,
Abstract: pig stress syndrome (pss) is a genetic disease caused by a nucleotide mutation in the ryr-2 gene encoding the ryanodine receptor (ryr-1), considered an autosomal recessive condition. pss, or malignant hyperthermia, is characterised by the lowering of meat quality and animal death, leading to a pale, smooth and exhudative (pse) carcass. the syndrome is triggered in genetically-susceptible individuals either by anaesthetic agents, like halothane, or stress conditions, such as transport, crowding and mating. information about this syndrome's incidence and presence in colombia is currently quite scarce. this study was aimed at determining the presence of the ryr-i t-allele in a porcine population from the universidad national de colombia's marengo farm to identify individuals which were more susceptible to the syndrome. a total of 50 pigs (27 males and 23 females) were selected at random. cc genotype frequency for males was 0.59, 0.37 for the ct genotype and 0.04 for the tt genotype; in females this was 0.65 for the cc genotype, 0.13 for ct and 0.22 for tt. a total of 31 (62 %) individuals were considered healthy (cc), 13 (26 %) were carriers (ct) and six (12 %) were susceptible (tt). c and t allele frequency was 0.75 and 0.25, respectively. hardy-weinberg equilibrium comparison tests revealed that the population was in genetic disequilibrium (p ≤ 0.05). the results did show the presence of the allele responsible for the syndrome; this is a very significant factor to be considered when establishing an appropriate animal breeding programme by using marker-assisted selection.
Immunophenotypical Switch versus Tumor Heterogeneity in a Patient with HIV-Associated Diffuse Large B-Cell Lymphoma
Jorge J. Castillo,Tina Rizack,Diana Treaba
Pathology Research International , 2011, DOI: 10.4061/2011/563216
Abstract: Patients with HIV/AIDS have a higher risk of developing aggressive B-cell lymphomas, such as diffuse large B-cell lymphoma (DLBCL). Lymphomas are rather heterogeneous in nature and in a few cases can switch their genetic or immunohistochemical phenotype, transform into other lymphomas or carry more than one malignant clone. In this report, we present the case of a 47-year-old man with HIV infection who was diagnosed with an apparent low-risk, early-stage DLBCL, but became refractory to therapy while undergoing treatment with rituximab-containing chemotherapy. We postulate that the development of his refractory disease occurred in the context of an immunohistochemical switch or the surge of a clone refractory to therapy. This phenomenon was not associated with a superinfection with EBV or HHV-8.
Estudio eco-epidemiológico de la tripanosomiasis americana en el municipio de Amalfi (Antioquia)
Martha Wolff,Diana Castillo,J. Arboleda
Iatreia , 2000,
Abstract: El departamento de Antioquia no ha sido considerado endémico para la enfermedad de Chagas, sin embargo en el nordeste antioque o, probablemente ocasionado por la tala y quema excesivas y la creciente deforestación para establecer medios de producción agrícola y ganadera, en las áreas naturales, se ha observado la aparición de insectos triatominos al interior de viviendas rurales, alimentándose de sus habitantes. Esta situación motivó la realización de un estudio ecoepidemiológico de Tripanosomiasis americana en nueve veredas del municipio de Amalfi con el propósito de identificar las especies de triatominos presentes en la zona, el grado de domiciliación, el índice de infección natural por Trypanosoma cruzi, los porcentajes de infección de animales domésticos (caninos) y de mamíferos silvestres, la determinación de la seroprevalencia humana y finalmente la identificación de las acciones del hombre que están llevando a los triatominos a las viviendas para refugio y alimentación. En las veredas estudiadas se capturaron 196 triatominos, consideradas especies silvestres: Panstrongylus geniculatus (49%), P. rufotuberculatus (47%) y Triatoma dispar (3%). El índice de infestación domiciliaria fue 14.53 % y las veredas con mayor infestación fueron Montebello (32.73 %) y La Gardenia (27.5 %). Igualmente teniendo en cuenta el total de insectos infectados naturalmente por T. cruzi, fueron estas dos veredas las que obtuvieron el mayor porcentaje de infección 43.75 % y 25.0 % respectivamente. En viviendas de la vereda La Gardenia se hallaron huevos y ninfas de P. geniculatus y P. rufotuberculatus, los cuales fueron corroborados por morfología en ninfas y adultos y por microscopía electrónica de barrido en huevos, teniendo como base de comparación huevos de colonias preestablecidas de ambas especies. Se capturaron 33 animales silvestres pertenecientes a géneros como: Didelphis, Marteja, Marmosa, Hoplomys), Dasypus, Proechymis, Poechinus, Oryzomis, Philander y Rodentia. Un 20.0% de ellos resultaron positivos para T. cruzi, mediante ELISA. En cuanto a los reservorios domésticos, el 47.6 % de las muestras de caninos fueron positivas por ELISA, el 10 % por ELISA e IFI. Al determinar la seroprevalencia humana se encontró en la vereda La Gardenia una mujer de 22 a os, que presentó positividad por T. cruzi para las pruebas de ELISA e IFI con títulos de 1:160, éste sería el primer caso de seropositividad confirmada para T. cruzi en Antioquia. Debido a la presencia T. cruzi en vectores, reservorios y en un humano y a las evidencias de reproducción intradomiciliaria, éste es el
Areas with Potential for Commercial Timber Plantations of Enterolobium cyclocarpum (Jacq.) Griseb. in Michoacán, México  [PDF]
Hipólito Jesús Mu?oz-Flores, J. Trinidad Sáenz-Reyes, Agustín Rueda-Sánchez, David Castillo-Quiroz, Francisco Castillo-Reyes, Diana Yemilet Avila-Flores
Open Journal of Forestry (OJF) , 2016, DOI: 10.4236/ojf.2016.65036
Abstract: Michoacán has a deforested area of 525,260 ha, representing 52,526 ha per year, mainly caused by anthropogenic disturbances such as agricultural burning and forest fires (50%), change of use of land for extensive livestock farming (28%), agriculture (17%) and illegal logging (5%). The establishment of forest plantations is an alternative for reducing the pressure on natural forests and creating options for sustainable development and diversification of production and conversion of land for agricultural and livestock fragmented for forestry purposes. The aim of this study was to determine the potential areas for commercial forest plantations of Enterolobium cyclocarpum (Jacq.) Griseb. in the State of Michoacán, México, through the use of Geographic information systems (GIS). Areas were identified using IDRISI 32, and ArcView software. Screening variables include annual temperature, annual precipitation, land use, soil type, elevation and slope. Products obtained were field verified. Two maps where potential areas for the establishment of commercial forest plantations of E. cyclocarpum shown were obtained. Potential areas for commercial forest plantation establishment are (0% - 15% slope) for commercial forest plantations mechanized was 57,227 ha and (15% - 30% slope) for manual plantations was 6273 ha. The total area in the state with potential for the establishment of the species in the study was 63,500 ha.
Efecto inmunosupresor de Trypanosoma lewisi (Kinetoplastidae) sobre la multiplicación de Toxoplasma gondii (Sarcocystidae) en macrófagos alveolares y peritoneales de rata blanca
Ríos Carrera,Nivia J; Chinchilla Carmona,Misael; Guerrero,Olga M; Castro Castillo,Alfredo;
Revista de Biología Tropical , 2009,
Abstract: the immunosuppressant effect of t. lewisi (kinetoplastidae) infection on the multiplication of toxoplasma gondii (sarcocystidae) on alveolar and peritoneal macrophages of the white rat. the immunosuppressant effect of t. lewisi infection on the multiplication of t. gondii was compared in peritoneal (mp) and alveolar macrophages (ma) of white rat. two animal groups were infected with t. lewisi and sacrificed after four days and seven days post infection. a group without infection was maintained as a control. the number of intracellular parasites (tachyzoites) (it) was counted by light microscopy, calculating the rate infection rate per 100 total cells (tc) and per infected cells (ic) for each group of phagocyte cells. the relation quotient it, tc or ic multiplied percent, provided a statistical ratio (re) of the relative number of parasites in both cellular types for each time interval. ma as well as mp obtained after 4 days showed a significant increase in the multiplication of t. gondii with respect to the control. unlike the mp (which had an increase in the multiplication of t. gondii the fourth day of infection with t. lewisi diminishing towards the seventh day), the ma had an increase in the multiplication of the parasite from the fourth to the seventh day. this difference can be related to the route of infection used for the experiments, that affect the mp directly with a greater effect in comparison with the ma of the lungs. lung compartment will be affected later, when the infection becomes systemic between the fourth and sixth day of infection. the immunity against t. gondii is similar between both phagocytes, but the time of infection and the compartment where the cells are located, makes the difference in the response time against t. gondii. supernatants from macrophage cultures or t. lewisi by rat did not induced any immunosuppression. rev. biol. trop. 57 (1-2): 13-22. epub 2009 june 30.
Efecto inmunosupresor de Trypanosoma lewisi (Kinetoplastidae) sobre la multiplicación de Toxoplasma gondii (Sarcocystidae) en macrófagos alveolares y peritoneales de rata blanca
Nivia J Ríos Carrera,Misael Chinchilla Carmona,Olga M Guerrero,Alfredo Castro Castillo
Revista de Biología Tropical , 2009,
Abstract: El efecto inmunosupresor de la infección de T. lewisi sobre la multiplicación de T. gondii fue comparado en macrófagos peritoneales (MP) y alveolares (MA) de rata. El número de parásitos (taquizoitos) intracelulares (TI) fue contado por microscopía de luz. Los macrófagos alveolares y peritoneales (MP) de animales con 4 días de infección con T. lewisi muestran un aumento significativo en la multiplicación de T. gondii. A diferencia de los MP (que muestran un aumento en la multiplicación de T. gondii al cuarto día de infección con T. lewisi disminuyendo hacia el séptimo día), los MA mantienen un aumento en la multiplicación del parásito desde el cuarto, aumentando hacia el séptimo día de infección. Esta diferencia se puede deber a la ruta de infección utilizada para los experimentos que afectan directamente los MP donde se observa un efecto mayor y más temprano en comparación con los MA aislados de los pulmones, compartimiento afectado cuando la infección se vuelve sistémica entre el cuarto y sexto día de infección. La inmunidad contra T. gondii es similar entre ambas células fagocíticas, pero el tiempo de infección y el compartimiento donde se encuentren las células hace la diferencia en el tiempo de respuesta contra un parásito dado, en nuestro caso T. gondii. No hubo evidencia de que los sobrenadantes de cultivos de macrófagos provenientes de ratas infectadas ni el lisado de tripanosomas indujeran el efecto inmunosupresor. The immunosuppressant effect of T. lewisi (Kinetoplastidae) infection on the multiplication of Toxoplasma gondii (Sarcocystidae) on alveolar and peritoneal macrophages of the white rat. The immunosuppressant effect of T. lewisi infection on the multiplication of T. gondii was compared in peritoneal (MP) and alveolar macrophages (MA) of white rat. Two animal groups were infected with T. lewisi and sacrificed after four days and seven days post infection. A group without infection was maintained as a control. The number of intracellular parasites (tachyzoites) (IT) was counted by light microscopy, calculating the rate infection rate per 100 total cells (TC) and per infected cells (IC) for each group of phagocyte cells. The relation quotient IT, TC or IC multiplied percent, provided a statistical ratio (RE) of the relative number of parasites in both cellular types for each time interval. MA as well as MP obtained after 4 days showed a significant increase in the multiplication of T. gondii with respect to the control. Unlike the MP (which had an increase in the multiplication of T. gondii the fourth day of infection with T. lewisi dimi
Porcine Respiratory Pathogens in Swine Farms Environment in Mexico  [PDF]
Victor M. Loera-Muro, Abraham Loera-Muro, Marcela Morfín-Mata, Mario Jacques, Francisco J. Avelar-González, Flor Ramírez-Castillo, Elsa M. Ramírez-López, Alma L. Guerrero-Barrera
Open Journal of Animal Sciences (OJAS) , 2014, DOI: 10.4236/ojas.2014.44025
Abstract: Respiratory pathogens are the main health problem in the swine industry worldwide. These pathogens are transmitted by direct contact between animals or by aerosols and however are not well known yet, if the environment works as its reservoir, inoculum and/or dispersion medium. The objective of this study was to determine the presence of respiratory pathogens in environmental samples from swine farms in Aguascalientes, Mexico, through of PCR and RT-PCR techniques. The bacteria Actinobacillus pleuropneumoniae and Pasteurella multocida were found viable in samples from water, food, soil and air. Streptococcus suis was found in a viable state in water samples. Haemophilus parasuis, Porcine Reproductive and Respiratory Syndrome virus and Swine Influenza virus (H1N1 and H3N2) were detected in drinking water samples. Mycoplasma hyopneumoniae and Porcine Circovirus type 2 (PCV2) were not detected in environmental samples. These results suggest that the environment of the farms acts as a reservoir, inoculum and/or vehicle of dispersion for these pathogens except for M. hyopneumoniae and PCV2.
Acute Myeloid Leukemia Evolving from JAK 2-Positive Primary Myelofibrosis and Concomitant CD5-Negative Mantle Cell Lymphoma: A Case Report and Review of the Literature
Diana O. Treaba,Salwa Khedr,Shamlal Mangray,Cynthia Jackson,Jorge J. Castillo,Eric S. Winer
Case Reports in Hematology , 2012, DOI: 10.1155/2012/875039
Abstract: Primary myelofibrosis (formerly known as chronic idiopathic myelofibrosis), has the lowest incidence amongst the chronic myeloproliferative neoplasms and is characterized by a rather short median survival and a risk of progression to acute myeloid leukemia (AML) noted in a small subset of the cases, usually as a terminal event. As observed with other chronic myeloproliferative neoplasms, the bone marrow biopsy may harbor small lymphoid aggregates, often assumed reactive in nature. In our paper, we present a 70-year-old Caucasian male who was diagnosed with primary myelofibrosis, and after 8 years of followup and therapy developed an AML. The small lymphoid aggregates noted in his bone marrow were neoplastic in nature and represented bone marrow involvement by a CD5-negative mantle cell lymphoma (MCL) that presented without any associated lymphadenopathy. We reviewed the English medical literature to identify a single case report of simultaneous association of AML and a MCL in the bone marrow. The unusual association presented here suggests an increase in observer awareness to apparently benign lymphoid aggregates in chronic myeloproliferative neoplasms.
Clinical applications of scintigraphy in small animals in Buenos Aires, Argentina
Soler,Elber A; Castillo,Víctor A; Calcagno,Fernando; Velandia,Diana J;
Revista Colombiana de Ciencias Pecuarias , 2011,
Abstract: scintigraphy (cgf) is a diagnostic imaging tool utilizing specific radiopharmaceuticals to assess the anatomical and functional status for each organ or tissue. this technology allows the veterinarian to anticipate, supplement or complete the diagnosis, and also to monitor the treatment. unfortunately, this diagnostic method is little known, and its lack of dissemination among clinical veterinarians prevents its optimal use. in order to understand the benefits of cgf as an effective imaging tool in the city of buenos aires during the years 2004-2006, we analyzed the studies that utilized cgf in canine and cats during this period. from a total of 142 studies, it was found that 38.6% of the assessments corresponded to thyroid disorders, while 61.4% of the remaining studies that involved cgf included, in descending order, pathologies associated with liver, bone, kidneys, parathyroid gland and lungs. the main findings in this paper highlight the use of cgf in the diagnosis of functional thyroid carcinoma followed by portosystemic shunt, bone neoplasms, evaluation of individual kidney function, parathyroid hyperplasia, parathyroid adenoma and pulmonary embolism.
Indicaciones y aplicaciones clínicas de la centellografía realizada en peque os animales en Buenos Aires, Argentina Indica es e aplica es clínicas da cintilografia de pequenos animais realizada em Buenos Aires, Argentina Clinical applications of scintigraphy in small animals in Buenos Aires, Argentina
Elber A Soler,Víctor A Castillo,Fernando Calcagno,Diana J Velandia
Revista Colombiana de Ciencias Pecuarias , 2011,
Abstract: La centellografía (CGF) es una ayuda diagnóstica a través de imágenes, que permite evaluar el estado anatómico-funcional por medio del empleo de radiofármacos específicos para cada órgano o tejido en los animales de compa ía, permitiendo anticipar, complementar o concluir un diagnóstico, al igual que permite hacer el seguimiento al tratamiento instaurado. Lamentablemente es un método diagnóstico muy poco conocido y difundido entre los clínicos veterinarios, llegando a omitirse su realización. Con el propósito de conocer los diferentes tipos de estudios, aportes e indicaciones que se obtuvieron mediante el uso de la cámara gamma en la ciudad de Buenos Aires durante los a os 2004-2006, se analizaron los informes de los estudios CGF que fueron realizados a caninos y felinos durante este periodo. Al finalizar el estudio se logro determinar que el 38.6% del total de los 142 estudios correspondía a evaluaciones de la glándula tiroides, mientras el 61.37% estaba constituido por cinco diferentes estudios entre los que se mencionan en orden decreciente, CGF hepática, ósea, renal, paratiroidea y pulmonar. Las principales indicaciones encontradas en el presente trabajo se destacó el diagnóstico de carcinoma de glándula tiroides funcional seguido por el shunt porto sistémico, neoplasias de tejido óseo, evaluación de la función renal individual, hiperplasia paratiroidea, adenoma paratiroides y embolismo pulmonar. A cintilografia (CGF) é um auxílio diagnóstico através de imagens, que avalia o estado anat mico e funcional através do uso de radiofármacos específicos para cada órg o ou tecido em animais domésticos, permitindo antecipar, complementar ou completar o diagnóstico, Também permite monitorar o tratamento iniciado. Infelizmente é um método de diagnóstico pouco conhecido e divulgado entre os clínicos veterinários. Com o propósito de compreender os diferentes tipos de estudos, aportes e indica es que foram realizados utilizando-se uma camara gamma, na cidade de Buenos Aires, durante o período de 2004-2006. Foram analisados os informes dos estudos de CGF que foram conduzidos para c es e gatos durante este período. No final do estudo foi determinado que o 38,6% do total de 142 estudos correspondem às avalia es da glandula tireóide, enquanto que 61,37% era composto por cinco diferentes estudos entre os listados em ordem decrescente, fígado, ósseo, renal , paratireóide e pulm o. As principais indica es encontrados neste trabalho destacaram o diagnóstico de carcinoma da tiróide funcional, seguido pelo shunt sistêmico, neoplasias ósseas, da fun o renal individual, h
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