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Search Results: 1 - 10 of 13547 matches for " Diana Bruno "
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Comparison of fluorescence polarization assay with Rose Bengal (RB) test and complement fixation tests for the diagnosis of buffalo (Bubalus bubalis) brucellosis in a high-prevalence area
S. Montagnaro,U. Pagnini,T. Diana,L. Bruno
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.s2.858
Abstract: The fluorescence polarisation assay (FPA) was evaluated for the serological diagnosis of bovine brucellosis in buffalo (Bubalus Bubalis) in southern Italy. This assay uses O-polysaccharide prepared from Brucella Abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. It has many methodological advantages over older, more established tests and can be performed in short time. To measure the fluorescence polarization, a Tecan GENios Pro (Prionics) fluorescence-polarization analyzer was used with the procedure described by Nielsen et al. 1996. A cut-off value of 117 millipolarization (mP) units was used for testing 912 buffalo sera from Campania Region (526 positive sera and 386 negative sera according to the complement fixation test; CFT). All samples were tested with the Rose Bengal plate (RB). Sensitivity and specificity (Sn) for RB were 84.0% and 87.8% and for FPA were 92.6% and 88.9 percent. The FPA gave a kappa coefficient of agreement with respect to CFT of 0.755, while RB (relative to the CFT) gave coefficients of 0,715. Finally, ROC analysis suggested a cut-off value which did not agree with the one recommended in the test procedure for cow.
Creatine HCl and Creatine Monohydrate Improve Strength but Only Creatine HCl Induced Changes on Body Composition in Recreational Weightlifters  [PDF]
Elias de Fran?a, Bruno Avelar, Caroline Yoshioka, Jeferson Oliveira Santana, Diana Madureira, Leandro Yanase Rocha, Cesar Augustus Zocoler, Fabrício Eduardo Rossi, Fabio Santos Lira, Bruno Rodrigues, érico Chagas Caperuto
Food and Nutrition Sciences (FNS) , 2015, DOI: 10.4236/fns.2015.617167
Abstract: Background: Creatine supplementation is a subject that is very well studied. New forms of creatine are suggesting improvements in this supplement performance. Creatine HCl is supposed to have better solubility and absorption than creatine. The aim of this study was to compare the effects of two different doses of creatine HCl with creatine monohydrate on the strength and body composition in recreational weightlifters and to verify the relationship between strength and body composition. Methods: 40 subjects were divided in four groups: Creatine Monohydrate (CMG) 5 g/daily; Creatine HCl-1 (HCl-1) 5 g/daily, Creatine HCl-2 (HCl-2) 1.5 g/daily and Control group (CG) = 5 g of resistant starch/daily. All groups performed a resistance training program during 4 weeks. Body composition and strength were evaluated pre and post intervention. Results: The 1 RM at the Leg press was increased significantly in all groups (CMG: pre = 264.4 ± 83.8 × post = 298.1 ± 90.9; HCl-1: pre = 295.0 ± 88.3 × post = 338.3 ± 86.8 and HCl-2: pre = 274.3 ± 57.1 × post = 305.7 ± 59.4; p< 0.05), Bench press 1 RM was increased significantly only in HCl-2 (pre = 72.4 ± 25.7 × post = 76.0 ± 25.0; p = 0.003), however, there was no statistically significant difference between groups. Fatmass was significantly decreased in HCl-1 (pre = 14.5 ± 8.0 × post = 13.3 ± 8.3; p = 0.034) and HCl-2 (pre = 13.8 ± 5.8 × post = 12.7 ± 5.6; p = 0.005) but fat-free mass was increased only in HCl-1 (pre = 52.2 ± 8.9 × post = 53.8 ± 8.9; p = 0.031), with no differences between groups again. We observed strong correlations between upper limb strength and fat mass (r = 0.93, p < 0.05), and between lower limb strength and FFM (r = 0.93, p < 0.05) only in HCl-1 group. Conclusions: We concluded that creatine HCl and creatine Monohydrate improve performance but only creatine HCl induces changes on body composition in recreational weightlifters with differences between creatine HCl doses.
An interview with Diana from Libya
International Journal of Inclusive Democracy , 2011,
Abstract: IJID Note:The interviewer will be referred below as JR and the interviewee as DL. Both the interviewer as well as the interviewee preferred not to reveal publicly their names for security reasons.JR’s Notes:In the 1980s, when Gaddafi’s policies veered to the left and nationalization of the economy became widespread, Diana father’s business was severely affected. So her own background is not what one might expect of someone who now is speaking up passionately against the NATO assault on Libya…Unfortunately, most mainstream western journalists seem interested only in hearing from anti-Gaddafi Libyans. So it is the fact that westerners continue to get a one-sided picture of Libya which helps support the case for NATO bombing. Here’s another side to the story…
Energy Drinks Do Not Improve Le Parkour Performance: A Randomized Controlled Study  [PDF]
Diana Madureira, Jeferson Oliveira Santana, Bruno Avelar, Cesar Augustus de Sousa Zocoler, Bruno Rodrigues, érico Chagas Caperuto
Food and Nutrition Sciences (FNS) , 2016, DOI: 10.4236/fns.2016.74035
Abstract: Le Parkour is a sport where the athletes transpose common day-to-day obstacles in the best possible way. This sport demands elements like strength, focus and decision making; elements supposedly affected by energy drinks, with performance improvement. The aim of this study was to investigate the effects of energy drink ingestion on Le Parkour athlete’s performance. Twelve male amateur athletes, mean age of 23.5 ± 2.74, took part in this randomized, double-blind, placebocontrolled study. Subjects were randomly assigned to drink: 250 ml of energy drink, 500 ml of energy drink or placebo. Forty minutes after the beverage consumption, they were evaluated in a specific circuit performance test (time to complete the circuit) followed by a perceived exertion and a circuit technical difficulty evaluation. We also evaluated heart rate in 3 different moments: rest, pre-circuit and post-circuit. All variables were collected in 3 different experimental days. Time, perceived exertion and technical difficulty were higher in the first day compared to the others. Heart rate did not show a difference when we compared the days. Drinking one or two cans of an energy drink did not improve performance of Le Parkour athletes in a sport specific test. It also did not change heart rate or perceived exertion.
Mechanical Properties of Organelles Driven by Microtubule-Dependent Molecular Motors in Living Cells
Luciana Bruno,Marcelo Salierno,Diana E. Wetzler,Marcelo A. Despósito,Valeria Levi
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0018332
Abstract: The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, respectively. In this work, we used single particle tracking to characterize the mechanical properties of motor-driven organelles during transport along microtubules. We tracked organelles with high temporal and spatial resolutions and characterized their dynamics perpendicular to the cytoskeleton track. The quantitative analysis of these data showed that the dynamics is due to a spring-like interaction between melanosomes and microtubules in a viscoelastic microenvironment. A model based on a generalized Langevin equation explained these observations and predicted that the stiffness measured for the motor complex acting as a linker between organelles and microtubules is ~ one order smaller than that determined for motor proteins in vitro. This result suggests that other biomolecules involved in the interaction between motors and organelles contribute to the mechanical properties of the motor complex. We hypothesise that the high flexibility observed for the motor linker may be required to improve the efficiency of the transport driven by multiple copies of motor molecules.
Ordered structure of the transcription network inherited from the yeast whole-genome duplication
Diana Fusco, Luigi Grassi, Bruno Bassetti, Michele Caselle, Marco Cosentino Lagomarsino
BMC Systems Biology , 2010, DOI: 10.1186/1752-0509-4-77
Abstract: We studied the evolution of transcriptional interactions of whole-genome duplicates, to understand how they are wired into the yeast regulatory system. Our work combines network analysis and modeling of the large-scale structure of the interactions stemming from the WGD.The results uncover the WGD as a major source for the evolution of a complex interconnected block of transcriptional pathways. The inheritance of interactions among WGD duplicates follows elementary "duplication subgraphs", relating ancestral interactions with newly formed ones. Duplication subgraphs are correlated with their neighbours and give rise to higher order circuits with two elementary properties: newly formed transcriptional pathways remain connected (paths are not broken), and are preferentially cross-connected with ancestral ones. The result is a coherent and connected "WGD-network", where duplication subgraphs are arranged in an astonishingly ordered configuration.An organism can respond to internal and environmental cues by the coordinated activation of large sets of genes through transcriptional regulation. This process can be described as a complex "network" of interactions, connecting binding sites of regulatory proteins (transcription factors, TFs) to regulatory DNA regions of their target genes [1]. Achieving an understanding of the evolutionary forces that shape the architecture of this network is fundamental for contemporary biology, where large-scale functional genomic data are increasingly accessible experimentally.In particular, gene duplication is among the most widespread mechanisms for evolutionary genomic innovations [2,3]. Duplication gives rise to the widespread existence of gene families, where members share a common ancestor. Gene duplication can occur with different functional consequences [4,5] at the scale of a single gene or a medium-sized genomic segment, but also of a whole genome [3,6-8]. Specifically, the rare but revolutionary events of whole-genome duplicatio
The relationship between executive functions and fluid intelligence in schizophrenia
María Roca,Facundo Manes,Diana Bruno,Agustín Ibá?ez,Teresa Torralva
Frontiers in Behavioral Neuroscience , 2014, DOI: 10.3389/fnbeh.2014.00046
Abstract: An enduring question is unity vs. separability of executive deficits resulting from impaired frontal lobe function. In previous studies, we have asked how executive deficits link to a conventional measure of fluid intelligence, obtained either by standard tests of novel problem-solving, or by averaging performance in a battery of novel tasks. For some classical executive tasks, such as the Wisconsin Card Sorting Test (WCST), Verbal Fluency, and Trail Making Test B (TMTB), frontal deficits are entirely explained by fluid intelligence. However, on a second set of executive tasks, including tests of multitasking and decision making, deficits exceed those predicted by fluid intelligence loss. In this paper we discuss how these results shed light on the diverse clinical phenomenology observed in frontal dysfunction, and present new data on a group of 15 schizophrenic patients and 14 controls. Subjects were assessed with a range of executive tests and with a general cognitive battery used to derive a measure of fluid intelligence. Group performance was compared and fluid intelligence was introduced as a covariate. In line with our previous results, significant patient-control differences in classical executive tests were removed when fluid intelligence was introduced as a covariate. However, for tests of multitasking and decision making, deficits remained. We relate our findings to those of previous factor analytic studies describing a single principal component, which accounts for much of the variance of schizophrenic patients' cognitive performance. We propose that this general factor reflects low fluid intelligence capacity, which accounts for much but not all cognitive impairment in this patient group. Partialling out the general effects of fluid intelligence, we propose, may clarify the role of additional, more specific cognitive impairments in conditions such as schizophrenia.
Role of enzymatic activity in muscle damage and cytotoxicity induced by Bothrops asper Asp49 phospholipase A2 myotoxins: are there additional effector mechanisms involved?
Diana Mora-Obando,Cecilia Díaz,Yamileth Angulo,José María Gutiérrez,Bruno Lomonte
PeerJ , 2015, DOI: 10.7717/peerj.569
Abstract: Viperid venoms often contain mixtures of Asp49 and Lys49 PLA2 myotoxin isoforms, relevant to development of myonecrosis. Given their difference in catalytic activity, mechanistic studies on each type require highly purified samples. Studies on Asp49 PLA2s have shown that enzyme inactivation using p-bromophenacyl bromide (p-BPB) drastically affects toxicity. However, based on the variable levels of residual toxicity observed in some studies, it has been suggested that effector mechanisms independent of catalysis may additionally be involved in the toxicity of these enzymes, possibly resembling those of the enzymatically inactive Lys49 myotoxins. A possibility that Lys49 isoforms could be present in Asp49 PLA2 preparations exists and, if undetected in previous studies, could explain the variable residual toxicity. This question is here addressed by using an enzyme preparation ascertained to be free of Lys49 myotoxins. In agreement with previous reports, inactivation of the catalytic activity of an Asp49 myotoxin preparation led to major inhibition of toxic effects in vitro and in vivo. The very low residual levels of myotoxicity (7%) and cytotoxicity (4%) observed can be attributed to the low, although detectable, enzyme remaining active after p-BPB treatment (2.7%), and would be difficult to reconcile with the proposed existence of additional catalytic-independent toxic mechanisms. These findings favor the concept that the effector mechanism of toxicity of Asp49 PLA2 myotoxins from viperids fundamentally relies on their ability to hydrolyze phospholipids, arguing against the proposal that membrane disruption may also be caused by additional mechanisms that are independent of catalysis.
Harmonizing Budgeting and Accounting: The Case of Italy  [PDF]
Adriana Bruno
Open Journal of Accounting (OJAcct) , 2014, DOI: 10.4236/ojacct.2014.31005
Abstract: Many reforms have been undertaken in recent years; indeed, scholars have concentrated their attention on ac-counting techniques, financial management, and the potential for adding tools to support managerial reform agendas [1-3]. This paper extends prior research by focusing on the manner of diffusion ongoing reform process, through the adoption of the new version of Financial Management Reform process model [3]. The objective of this research is to investigate how a new accounting system has been introduced in the public sector in a processual and outcome-oriented analysis [4]. The study setting for this research is the Italian government, specifically the intermediate or regional level government, Campania Region, where a trial period is underway. The objective is to discover what obstacles and difficulties might exist when implementing a harmonized accounting and budgeting system while moving toward an accrual accounting system. The study reveals the main paradox in the implementation of the reform. This kind of study could add fresh knowledge to the literature and highlight common problems encountered when implementing accounting reforms. Additionally it could help set a standard to assist public entities in developing and implementing accounting guidelines.
A Few Reflections on the Reasons Why Cooperative Firms Have Failed to Gain a Firm Foothold  [PDF]
Bruno Jossa
Open Journal of Business and Management (OJBM) , 2015, DOI: 10.4236/ojbm.2015.33027
Abstract: After the inception of the cooperative movement in the mid-19th century, it was widely held that capitalistic businesses would soon be replaced by a system of worker-controlled firms and a wealth of policy proposals called for a major impulse to cooperation in later years also. But cooperation has not met with the hoped-for success. How do we account for this? To our day, employee management specialists have been unable to provide a satisfactory explanation for the fact that the policy proposal to introduce democratic firm control still carries little consensus despite the collapse of the Soviet model of communism. In this paper, we give some answers to the question and emphasise the idea that progress in the direction of a generalised system of cooperative firms would amount to a considerable improvement over capitalism, but will hardly be made without the effective contribution of intellectuals and political parties.
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