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Search Results: 1 - 10 of 76563 matches for " David; Rodríguez Leyes "
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Caracterización preliminar del aceite obtenido del fruto completo de Roystonea regia (Kunth) O. F. Cook
Rodríguez Leyes,Eduardo A.; González Canavaciolo,Víctor L.; Marrero Delange,David; Adames Fajardo,Yuliamny; Vicente Murillo,Roxana;
Revista Cubana de Plantas Medicinales , 2008,
Abstract: introduction: the cuban royal palm tree oil, roystonea regia (kunth) o.f. cook, has possibilities to be used as raw material in the cuban pharmaceutical industry. objectives: to characterize the cuban royal palm tree oil and to study its fatty acid composition. methods: the official methods described in usp 27 were used and the composition of fatty acids was estimated by gas chromatography. results: relative density was 0.874 - 0.970 g/ml; refraction index 1.454 - 1.463; the saponification index ranged 190.0 to 221.8; the acidity index ranged 5.2 to 26.4; iodine index was 49.2 to 55.8; and the contents of non-saponificable material ranged 2.0 to 13.4 %. the total of fatty acids in the analyzed oils varied from 74.4 to 89.4 %. the composition of fatty acids standardized at 100 % was given by the following concentrations: c8:0 y c10:0 (0.1-0.9 %); c12:0 (17.9-27.8 %); c14:0 (9.3-12.7 %); c16:0 (9.3-15.0 %); c16:1 (0.1-0.6 %); c18:0 (2.5-3.7 %); c18:1 (27.3-37.3 %); c18:2 (12.8-17.4 %); y c18:3 (0.1-0.5 %). conclusions: some of the physical and chemical characteristics of r. regia fruit oil were studied in this paper.
Contenidos de aceite y de los ácidos láurico y mirístico en frutos maduros de Roystonea regia colectados durante dos a?os
Rodríguez Leyes,Eduardo A.; González Canavaciolo,Víctor L.; Marrero Delange,David; Adames Fajardo,Yuliamny; Vicente Murillo,Roxana;
Revista Cubana de Plantas Medicinales , 2010,
Abstract: introduction: d004, an promissory active ingredient in treatment and prevention of begnin prostatic hyperplasia, is obtained from the fruit oil of roystonea regia (kunth) o.f. cook. it is composed by a mixture of free fatty acids including lauric and myristic acids, both very important by their pharmacological effects. objective: to determine the potential influence of harvest season on the oily content and the concentration of lauric and myristic acids from r. regia fruits. methods: each month for two years, it was possible to collect mature fruits from r. regia in a selected group. in dry and milled samples it was determined the oily content in a gravimetric way and that of fatty acids by gas chromatography. results: there were differences statistically significant in oily contents and of lauric and myristic acids; however, the contents determined in all the cases remained within the quality specifications established for the plant material. conclusions: the plant material collected for all the year may be used in obtaining of r. regia oil, raw material used in the production of d004.
Fracciones lipídicas obtenidas a partir de frutos de Serenoa repens recolectados en Cuba
Rodríguez Leyes,Eduardo A; González Canavaciolo,Víctor L; Marrero Delange,David; Leiva Sánchez,ángela T;
Revista Cubana de Plantas Medicinales , 2012,
Abstract: introduction: lipid extracts from the fruits of serenoa repens, a native palm from the united states of america, are used to treat benign prostate hyperplasia. although this species was introduced in the national botanic garden of cuba some years ago, the lipid extracts of its fruits had not been studied yet. objective: to determine the main organoleptic, physical and chemical characteristics of lipid extracts from the fruits of s. repens harvested in cuba. methods: dried fruits and fresh ripe fruits of s. repens were harvested. fresh fruits were dried at room temperature and at 80 oc. the three groups of dried fruits were grounded and extracted with hexane in s?xhlet equipment. the main characteristics of the dried lipid extracts were determined. the studied components were determined by gas chromatography, in some cases with mass spectrometry detection. results: organoleptic and physical characteristics as well as the contents of fatty acids (major components) and ethyl esters of the studied extracts agreed with that described in literature, whereas those of sterols and fatty alcohols were higher than those previously reported for this species. some fatty alcohols not previously reported in these extracts, that is, 1-eicosanol, 1-docosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol and 1-tetratriacontanol.were also identified. conclusions: generally speaking, the lipid extracts from the fruits of s. repens harvested in cuba presented characteristics similar to those reported for the extracts of this species, although some differences were also found, such as the content of sterols and fatty alcohols and the presence of some fatty alcohols not previously reported in the literature.
Determinación de alcoholes grasos en el ingrediente activo D004 por cromatografía de gases-espectrometría de masas
Marrero Delange,David; Rodríguez Leyes,Eduardo Antonio; González Canavaciolo,Víctor Luis; Adames Fajardo,Yuliamny; Vicente Murillo,Roxana;
Revista Cubana de Plantas Medicinales , 2012,
Abstract: introduction: d004, a new lipid extract purified from the cuban royal palm (roystonea regia [kunth] f. cook) fruits, has been shown to be effective in experimental models of prostate hyperplasia, and to exhibit antioxidant and anti-inflammatory effects. objectives: to identify the fatty alcohols that could be present in d004 active ingredient using gas chromatography-mass spectrometry. methods: samples of nine batches of d004 were submitted to saponification with koh/etoh solution and the unsaponifiable fractions were extracted with n-hexane. the alcohols were analyzed as trimetilsilil derivatives, which were obtained by reaction with mstfa. the quantification was carried out by the internal standard method using 1-eicosanol. results: the total content of alcohols in d004 ranged from 0.18 to 5.10 %. this fraction was mainly composed of 1-octacosanol (52.94 %), 1-triacontanol (25.91 %), and 1-hexacosanol (14.40 %); whereas the minority components were 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol 1-tetratriacontanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-octadecanol and 1-eicosanol were minor components, being the last six alcohols present in trace levels. conclusions: fatty alcohols were identified and quantified in d004 active ingredient by gas chromatography-mass spectrometry. the major components were 1-octacosanol, 1-triacontanol and 1-hexacosanol.
Determinación de esteroles en la fracción insaponificable del aceite de los frutos de la palma real cubana (Roystonea regia) Determination of sterols determination in unsaponifiable fraction from Cuban royal palm (Roystonea regia) fruit oil
David Marrero Delange,Eduardo Antonio Rodríguez Leyes,Víctor Luis González Canavaciolo,Carmen Luisa Morales Rico
Revista Cubana de Farmacia , 2013,
Abstract: Introducción: los esteroles han sido ampliamente estudiados por su importancia nutricional y farmacéutica. Sin embargo, para nuestro conocimiento, la composición de esteroles del aceite de los frutos de la palma real cubana (Roystonea regia) no ha sido determinada hasta el momento. Objetivos: determinar la composición de esteroles que pudieran estar presentes en la fracción insaponificable del aceite de R. regia mediante CG-EM. Métodos: muestras de dos lotes de aceite de R. regia fueron sometidas al procedimiento para la determinación de esteroles establecido por el del Instituto de Nutracéuticos de los EE.UU., el cual consiste, fundamentalmente, en una saponificación con disolución de KOH/EtOH y posterior extracción con n-hexano de las fracciones insaponificables. Las fracciones obtenidas fueron analizadas por CG-EM como derivados TMS e identificadas por comparación de sus espectros con los de patrones comerciales y los de la base de espectros Wiley. Para la cuantificación se utilizó el colestano como patrón interno. Resultados: en la fracción insaponificable del aceite de R. regia se encontró un contenido total de esteroles de 66,1%; lo que representa un 0,14 % del aceite. La fracción de esteroles, estuvo compuesta principalmente por a -sitosterol (51,2 %), estigmasterol (9,6 %), campesterol (9,2 %), 24-metilen-cicloartanol (9,2 %), 5-avenasterol (8,9 %), cicloartanol (7,5 %); además de otros componentes minoritarios como cicloartenol, -sitosterol y colesterol. Conclusiones: se identificaron y se cuantificaron mediante CG-EM los esteroles de la fracción insaponificable del aceite de R. regia, en la cual el a-sitosterol resultó el componente mayoritario. Estos resultados son una contribución al estudio de la composición química de dicho aceite y pudieran avalar su posible utilidad nutricional y seguridad. Introduction: sterols have been widely studied because of their pharmaceutical and nutritional importance. However, to our knowledge, the sterol composition of the oil from the Cuban Royal Palm fruits (Roystonea regia (Kunth) F. Cook), has not been yet identified Objectives: to determine by GC-MS the sterols that could be present in the unsaponifiable fraction of R. regia oil. Methods: samples of two batches of R. regia oil were subjected to the established procedure of the US Institute for Nutraceutical Advancement to determine sterols, which mainly consists of saponification with KOH/EtOH solution and a subsequent extraction of the unsaponifiable fractions by using n-hexane. These fractions were analyzed by GC-MS as TMS derivatives and they were id
Fracciones lipídicas obtenidas a partir de frutos de Serenoa repens recolectados en Cuba Lipid fractions obtained from fruits of Serenoa repens harvested in Cuba
Eduardo A Rodríguez Leyes,Víctor L González Canavaciolo,David Marrero Delange,ángela T Leiva Sánchez
Revista Cubana de Plantas Medicinales , 2012,
Abstract: Introducción: los extractos lipídicos de los frutos de Serenoa repens, una palma nativa de los EE. UU., se usan para tratar la hiperplasia prostática benigna. Aunque esta especie fue introducida en el Jardín Botánico Nacional de Cuba hace algunos a os, los extractos lipídicos de sus frutos aún no habían sido estudiados. Objetivo: determinar las principales características organolépticas, físicas y químicas de extractos lipídicos obtenidos de frutos de S. repens recolectados en Cuba. Métodos: se recolectaron frutos secos y frutos maduros frescos de S. repens, los últimos se secaron a temperatura ambiente y a 80 oC. Los 3 grupos de frutos secos se molieron y extrajeron con hexano en S xhlet. Los extractos lipídicos se secaron y se determinaron sus principales características. Los componentes estudiados se determinaron mediante cromatografía de gases, en algunos casos con detección por espectrometría de masas. Resultados: los extractos presentaron características organolépticas y físicas, así como contenidos de ácidos grasos (componentes mayoritarios) y ésteres etílicos que coincidieron con lo descrito en la literatura, mientras que los contenidos de esteroles y alcoholes grasos resultaron superiores a los publicados antes para esta especie. También se identificaron alcoholes grasos no encontrados previamente en estos extractos: 1-eicosanol, 1-docosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol y 1-tetratriacontanol. Conclusiones: de manera general, los extractos lipídicos de frutos de S. repens recolectados en Cuba presentaron características similares a las reportadas para los extractos de esta especie, aunque se encontraron algunas diferencias, como son mayores contenidos de esteroles y alcoholes grasos, y la presencia de algunos alcoholes grasos no identificados antes. Introduction: lipid extracts from the fruits of Serenoa repens, a native palm from the United States of America, are used to treat benign prostate hyperplasia. Although this species was introduced in the National Botanic Garden of Cuba some years ago, the lipid extracts of its fruits had not been studied yet. Objective: to determine the main organoleptic, physical and chemical characteristics of lipid extracts from the fruits of S. repens harvested in Cuba. Methods: dried fruits and fresh ripe fruits of S. repens were harvested. Fresh fruits were dried at room temperature and at 80 oC. The three groups of dried fruits were grounded and extracted with hexane in S xhlet equipment. The main characteristics of the dried lipid extracts were determined. The studied components were determ
Determinación de alcoholes grasos en el ingrediente activo D004 por cromatografía de gases-espectrometría de masas Determination of fatty alcohols in D-004 active ingredient by using gas chromatography-mass spectrometry
David Marrero Delange,Eduardo Antonio Rodríguez Leyes,Víctor Luis González Canavaciolo,Yuliamny Adames Fajardo
Revista Cubana de Plantas Medicinales , 2012,
Abstract: Introducción: el D004, nuevo extracto lipídico purificado a partir de los frutos de la palma real cubana (Roystonea regia [Kunth] F. Cook), ha mostrado ser efectivo en modelos experimentales de hiperplasia prostática, y ha mostrado efectos antioxidantes y antiiflamatorios. Objetivos: identificar los alcoholes que pudieran estar presentes en el ingrediente activo D004 utilizando la cromatografía de gases acoplada a espectrometría de masa. Métodos: muestras de 9 lotes de D004 se sometieron a saponificación con una disolución de KOH/EtOH y las fracciones insaponificables fueron extraídas con n-hexano. Los alcoholes se analizaron como derivados trimetilsilil, obtenidos por reacción con N-metil, N-trimetilsilil trifluoroacetamida. La cuantificación se basó en el empleo de 1-eicosanol como patrón interno. Resultados: el D004 presenta un contenido total de alcoholes entre 0,18 y 5,10 %. Esta fracción está compuesta principalmente por 1-octacosanol (52,94 %), 1-triacontanol (25,91 %) y 1-hexacosanol (14,40 %); y presenta como componentes minoritarios: 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol, 1-tetratriacontanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-octadecanol y 1-eicosanol, los seis últimos en niveles de trazas. Conclusiones: se identificaron y se cuantificaron mediante cromatografía de gases acoplada a espectrometría de masa los alcoholes grasos en el ingrediente activo D004. Los componentes mayoritarios fueron 1-octacosanol, 1-triacontanol y 1-hexacosanol. Introduction: D004, a new lipid extract purified from the Cuban royal palm (Roystonea regia [Kunth] F. Cook) fruits, has been shown to be effective in experimental models of prostate hyperplasia, and to exhibit antioxidant and anti-inflammatory effects. Objectives: to identify the fatty alcohols that could be present in D004 active ingredient using gas chromatography-mass spectrometry. Methods: samples of nine batches of D004 were submitted to saponification with KOH/EtOH solution and the unsaponifiable fractions were extracted with n-hexane. The alcohols were analyzed as trimetilsilil derivatives, which were obtained by reaction with MSTFA. The quantification was carried out by the internal standard method using 1-eicosanol. Results: the total content of alcohols in D004 ranged from 0.18 to 5.10 %. This fraction was mainly composed of 1-octacosanol (52.94 %), 1-triacontanol (25.91 %), and 1-hexacosanol (14.40 %); whereas the minority components were 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-no
Estudio de estabilidad acelerada del ingrediente activo D-004 en diferentes envases Accelerated stability study of D-004 active ingredient in different containers
Eduardo A. Rodríguez Leyes,Roxana C. Sierra Pérez,Víctor L. González Canavaciolo,David Marrero Delange
Revista Cubana de Plantas Medicinales , 2009,
Abstract: INTRODUCCIóN: D-004 es un nuevo ingrediente activo en fase de investigación y desarrollo, obtenido de los frutos de Roystonea regia (Kunth) O.F. Cook. OBJETIVO: precisar la estabilidad de D-004 en condiciones aceleradas en frascos de vidrio ámbar, de tereftalato de polietileno y de polietileno de alta densidad. MéTODOS: muestras de 3 lotes se almacenaron a (40 ± 2) oC y (75 ± 5) % de humedad relativa durante 12 meses. Se determinaron las características organolépticas, la densidad relativa, el índice de refracción (USP 27) y el contenido de ácidos grasos mediante cromatografía de gases. RESULTADOS: los parámetros medidos se mantuvieron dentro de las especificaciones de calidad establecidas, con excepción del contenido de ácidos grasos, que en los frascos de tereftalato y polietileno disminuyó significativamente a los 3, 6 y 4,5 meses, respectivamente. CONCLUSIONES: el vidrio ámbar protege al D-004 de la degradación más que los envases plásticos estudiados. INTRODUCTION: D-004 is a new active ingredient under research and development stage, which is obtained from Roystonea regia (Kunth) O. F. Cook fruits. OBJECTIVE: to precise over the D-004 stability under accelerated conditions in amber glass, polyethylene terephtalate and high density polyethylene containers. METHODS: samples from three batches were stored at (40 ± 2) oC and (75 ± 5) % relative humidity for 12 months. Organoleptic characteristics, relative density, refractive index (USP 27) and fatty acids content by gas chromatography, were determined. RESULTS: measured parameters were within the set quality specifications , except for fatty acids content that significantly decreased in polyethylene terephtalate and high density polyethylene containers after 3, 4 and a half and 6 months, respectively. CONCLUSIONS: the amber glass protects more D-004 from degradation than the analyzed plastic containers.
Contenidos de aceite y de los ácidos láurico y mirístico en frutos maduros de Roystonea regia colectados durante dos a os Oily contents and the lauric and myristic acids in mature fruits of Roystonea regia harvested for two year
Eduardo A. Rodríguez Leyes,Víctor L. González Canavaciolo,David Marrero Delange,Yuliamny Adames Fajardo
Revista Cubana de Plantas Medicinales , 2010,
Abstract: INTRODUCCIóN: el D004, un ingrediente activo promisorio en el tratamiento de la hiperplasia prostática benigna, se obtiene a partir del aceite del fruto de Roystonea regia (Kunth) O.F. Cook. Está compuesto por una mezcla de ácidos grasos libres que incluye los ácidos láurico y mirístico, ambos de gran interés por sus efectos farmacológicos. OBJETIVO: determinar la posible influencia de la época de colecta sobre el contenido de aceite y la concentración de los ácidos láurico y mirístico de los frutos de R. regia. MéTODOS: se colectaron cada mes, durante 2 a os, frutos maduros de R. regia en una población seleccionada. En las muestras secas y molidas se determinó el contenido de aceite gravimétricamente y el de ácidos grasos por cromatografía de gases. RESULTADOS: se encontraron diferencias estadísticas significativas en los contenidos de aceite y de los ácidos láurico y mirístico; no obstante, los contenidos determinados en todos los casos se mantuvieron dentro de las especificaciones calidad establecidas para el material vegetal. CONCLUSIONES: el material vegetal colectado durante todo el a o puede ser empleado en la obtención del aceite de R. regia, materia prima empleada en la producción de D004. INTRODUCTION: D004, an promissory active ingredient in treatment and prevention of begnin prostatic hyperplasia, is obtained from the fruit oil of Roystonea regia (Kunth) O.F. Cook. It is composed by a mixture of free fatty acids including lauric and myristic acids, both very important by their pharmacological effects. OBJECTIVE: to determine the potential influence of harvest season on the oily content and the concentration of lauric and myristic acids from R. regia fruits. METHODS: each month for two years, it was possible to collect mature fruits from R. regia in a selected group. In dry and milled samples it was determined the oily content in a gravimetric way and that of fatty acids by gas chromatography. RESULTS: there were differences statistically significant in oily contents and of lauric and myristic acids; however, the contents determined in all the cases remained within the quality specifications established for the plant material. CONCLUSIONS: the plant material collected for all the year may be used in obtaining of R. regia oil, raw material used in the production of D004.
Caracterización preliminar del aceite obtenido del fruto completo de Roystonea regia (Kunth) O. F. Cook Preliminary characterization of the oil from the whole fruit of Roystonea regia (Kunth) O. F. Cook
Eduardo A. Rodríguez Leyes,Víctor L. González Canavaciolo,David Marrero Delange,Yuliamny Adames Fajardo
Revista Cubana de Plantas Medicinales , 2008,
Abstract: INTRODUCCIóN: el aceite de la palma real cubana, Roystonea regia (Kunth) O.F. Cook, tiene perspectivas de aplicación como materia prima, en la industria farmacéutica cubana. OBJETIVOS: caracterizar el aceite de palma real cubana y estudiar su composición de ácidos grasos. MéTODOS: se emplearon métodos oficiales descritos en la USP 27. La composición de ácidos grasos se determinó mediante cromatografía de gases. RESULTADOS: la densidad relativa estuvo entre 0,874 y 0,970 g/mL; el índice de refracción entre 1,454 y 1,463; el índice de saponificación entre 190,0 y 221,8; el índice de acidez entre 5,2 y 26,4; el índice de yodo entre 49,2 y 55,8; y el contenido de material no saponificable entre 2,0 y 13,4 %. El total de ácidos grasos en los aceites analizados varió entre 74,4 y 89,4 %. La composición de ácidos grasos, normalizada 100 %, estuvo dada por las concentraciones siguientes: C8:0 y C10:0 (0,1-0,9 %); C12:0 (17,9-27,8 %); C14:0 (9,3-12,7 %); C16:0 (9,3-15,0 %); C16:1 (0,1-0,6 %); C18:0 (2,5-3,7 %); C18:1 (27,3-37,3 %); C18:2 (12,8-17,4 %); y C18:3 (0,1-0,5 %). CONCLUSIONES: se estudiaron algunas de las características físicas y químicas del aceite obtenido de los frutos enteros de R. regia. INTRODUCTION: the Cuban royal palm tree oil, Roystonea regia (Kunth) O.F. Cook, has possibilities to be used as raw material in the Cuban pharmaceutical industry. OBJECTIVES: to characterize the Cuban royal palm tree oil and to study its fatty acid composition. METHODS: the official methods described in USP 27 were used and the composition of fatty acids was estimated by gas chromatography. RESULTS: relative density was 0.874 - 0.970 g/mL; refraction index 1.454 - 1.463; the saponification index ranged 190.0 to 221.8; the acidity index ranged 5.2 to 26.4; iodine index was 49.2 to 55.8; and the contents of non-saponificable material ranged 2.0 to 13.4 %. The total of fatty acids in the analyzed oils varied from 74.4 to 89.4 %. The composition of fatty acids standardized at 100 % was given by the following concentrations: C8:0 y C10:0 (0.1-0.9 %); C12:0 (17.9-27.8 %); C14:0 (9.3-12.7 %); C16:0 (9.3-15.0 %); C16:1 (0.1-0.6 %); C18:0 (2.5-3.7 %); C18:1 (27.3-37.3 %); C18:2 (12.8-17.4 %); y C18:3 (0.1-0.5 %). CONCLUSIONS: some of the physical and chemical characteristics of R. regia fruit oil were studied in this paper.
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