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Search Results: 1 - 2 of 2 matches for " Dararad Choosawad "
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Anti-tumour activities of fucoidan from the aquatic plant Utricularia aurea lour
Dararad Choosawad,Ureporn Leggat,Chavaboon Dechsukhum,Amornrat Phongdara
Songklanakarin Journal of Science and Technology , 2005,
Abstract: Fucoidan, a sulfated polysaccharide with several biological activities, is usually isolated from marine seaweeds or from echinoderms. Here, we report on the anti-tumour activity of fucoidan isolated from the aquatic plant Utricularia aurea Lour (Lentibulariaceae). A crude extract (CE) prepared by incubating U.aurea with hot water at 95oC for 12 hr was partially purified by Sephadex G-50, eluting with a 50mM sodium acetate buffer, at pH 5.0, containing 0.5M NaCl. Partially purified fucoidan (PPF) had a 3- fold increase in fucose content when compared with the CE and a molecular weig t of 11.6 kDa as determined by Sephadex G-200. Chemical analysis showed that CE consisted of 62.5% glucuronic acid, 5.0% fucose, 1.7% sulfate and 12.0% proteins while PPF consisted of 65.0% glucuronic acid, 15.3% fucose, 2.1 % sulfate and 8.3% proteins.The anti-tumour activity of the CE and PPF was determined by the MTT test. The CE at 125 μg/mL fucoidan and PPF at 250 μg/mL inhibited the growth of KB cells (a nasopharynx tumour cell line), but did not inhibit that of normal fibroblast cells. The inhibition was postulated to occur via apoptosis as significantlymore apoptotic cells were found after treatment than in the untreated KB cells (P<0.05) by the TUNNEL (TdT-mediated dUTP Nick-End Labelling) assay.
Biological activity of fucoidan from leafy bladderwort (Utricularia aurea Lour.)
Choosawad, D.,Phongdara, A.,Chotigeat, W.
Songklanakarin Journal of Science and Technology , 2005,
Abstract: Fucoidan extracted from aquatic plant Utricularia aurea was 1.3% of dry weight and comprised glucuronic acid 62.5% and fucose 4.98% of the crude extract and 28.74% of the fucose was sulfate. The fucoidan was investigated for antibacterial activity. The minimal inhibitory concentration (MIC) of crude fucoidan against Vibrio harveyi and Escherichia coli was 20 and 10 mg/ml. Moreover, the fucoidan had anticoagulant activity. The crude fucoidan had activated partial thromboplastin time (APTT) at 4.47 IU/mg.
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