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Behavioural and Neurophysiological Effects of a Stroke Rehabilitation Program on Emotional Processing in Tuberothalamic Infarct—Case Study  [PDF]
Katarzyna Kucharska, Ewelina Wilkos, Roman Stefanski, Grzegorz Makowicz, Danuta Ryglewicz, Ksenia Slawinska, Ewa Piatkowska-Janko
Journal of Behavioral and Brain Science (JBBS) , 2016, DOI: 10.4236/jbbs.2016.61006
Abstract: The aim of the study was to assess the neurophysiological and behavioural effects of a stroke rehabilitation involving Treatment As Usual (TAU) combined with Social Cognitive Training (SCT) in a right-handed patient suffered from tuberothalamic infarct compared to healthy controls (HCs) (n = 13). Methods: Both HCs and the patient were assessed by means of the following measures: Penn Emotion Perception Battery (ER40, EmoDiff40, PEAT40, PFMT), Reading the Mind in the Eyes Test, and Toronto Alexithymia Scale alongside clinical scales (Mini Mental State Examination, The State-Trait Anxiety Inventory, and Hamilton Depression Scale). The SCT was delivered individually for 60 minutes weekly in a 12-week program (12 sessions). The subject participated twice in a fMRI scanning session including the event-related task of implicit processing of 100% fearful expressions to detect physiological changes after TAU plus SCT and compared them with HCS who underwent the same assessment once. Results: Compared with HCs, the patient before therapy revealed lower scores in emotion recognition; particularly perception of anger was affected alongside worse performance on both emotion discrimination and acuity tests. After therapy, B.D. showed improvement in emotional processing. B.D. had less post-therapy activation maps compared with pretherapy ones and more significantly activated pre-and post-central gyrus and right cerebellum in response to fearful faces. Interestingly, no amygdala was significantly activated as the response to fearful stimuli before or after therapy was completed. Conclusions: Further research was needed to increase understanding about efficacy of SCT and the theory of neuroplasticity, thus helping rehabilitation programs.
Regeneration of Paeonia mlokosewitschii Lom. and P. tenuifolia L. in vitro from different explants
Teresa Orlikowska,Agnieszka Marasek,Danuta Kucharska
Acta Societatis Botanicorum Poloniae , 1998, DOI: 10.5586/asbp.1998.026
Abstract: The pattern of regeneration from tissues of Paeonia mlokosewitschii and P. tenuifolia cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spherical structures within 1.5 years. After that time also from those cultures arised nodular, shoot regenerating callus developed.
Properties and Structure of Microcrystalline Chitosan and Hydroxyapatite Composites  [PDF]
Luciano Pighinelli, Magdalena Kucharska
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2014, DOI: 10.4236/jbnb.2014.52015

The skeletal system in the human body is very important, provides support and gives shape to the body and provides a network between all soft tissues. The most common problems in hard tissues are bone fractures, defects or diseases which needed to be treated. The developments in artificial bone area seem to solve most of the hard tissue problems, on the other hand artificial bones themselves may cause other problems and in many cases they do not have sufficient mechanical properties and/or good biocompatibility. The importance of chitosan and its derivatives like microcrystalline chitosan has grown significantly over the last two decades due to its renewable and biodegradable source, and also because of the increase in the knowledge of its functionality in the technological and biomedical applications. The excellent biocompability, biofunctionality, and non-antigenic property make the chitosan and its derivatives as a microcrystalline chitosan an ideal material for tissue regeneration. To improve the suitability of chitosan for bone tissue engineering, the composites of MCCh and hydroxyapatite were studied. In the present work the characterization of the MCCh and composites with HAp in form of films and sponges, is based on physico-chemical tests, morphology, structure, particle size of HAp powder and distribution in the polymer matrix. The compositions with film and sponge shape are derived from the junction of two different materials, containing organic and inorganic substances. All sponge preparations, with HAp/MCCh have a well-shaped 3-dimensional structure, which could be used as implants in orthopedic surgery for a scaffolds base for medical applications.

Properties of Microcrystalline Chitosan-Calcium Phosphate Complex Composite  [PDF]
Luciano Pighinelli, Magdalena Kucharska
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2013, DOI: 10.4236/jbnb.2013.44A003

Nature itself uses materials like, cellulose to provide the structure of plants, chitin as the exoskeleton of several insects and molluscs, collagen for mechanical support in connective tissues and so on. At present, the socioeconomic situation of the modern world has raised the interest in renewable materials being used in regenerative medicine. The composition of MCCh/?-TCP complex in sponge shape is derived from the junction of two or more different materials, containing organic and inorganic materials, including bioactivity and biodegradability as a characteristic. The chemical characteristics of MCCh/?-TCP complex composites showed that both of the components organic and inorganic exist in the material. All sponge preparations, with MCCh/?-TCP have a well-shaped 3-dimentional structure, a highly porous and interconnected and homogenous pore structure to ensure a biological environment conducive to cell attachment and proliferation as well as tissue growth, providing the passage of nutrient flow. These materials can be used in future for medical applications as a base for scaffolds production and as implants in regenerative medicine.

Influence of Ageing with UV Radiation on Physicochemical Properties of Acrylic-Polyurethane Coatings  [PDF]
Danuta Kotnarowska
Journal of Surface Engineered Materials and Advanced Technology (JSEMAT) , 2018, DOI: 10.4236/jsemat.2018.84009
Abstract: Investigated systems of acrylic-polyurethane coatings consisted of three kinds of coatings: base coat (acrylic), intermediate (polyurethane) and top coat (acrylic); each made up of two layers. Mean thickness of the coating system was equal (145 ± 1) μm. In time of accelerated UV ageing, electromagnetic radiation was emitted of wave length in the range (300 - 400) nm which was progressively inducing a destruction of coatings chemical structure, especially photooxidation and photolysis of coating material. Carried out X-ray investigations revealed increased contents of oxygen in aged coatings due to their photooxidation which resulted in increased tendency of coating microfragments to chip off from surface layer with the ageing time flow. The hardness increase of UV aged coatings was also observed which intensified their surface layer brittleness. This contributed to their thickness decrease by more than 10% after 2016 h of ageing. Photodestruction of UV aged coatings was documented as well by characteristics obtained during investigations carried out with DMA method use. A noticeable physical destruction of the coatings was observed in the form of silver cracks, etchings and grooves in surface layer as well as craters extending also into interlayer. Processes of physical and chemical destruction undergoing in aged coatings influenced roughness profile change and coatings surface topography shaping. After 2016 h of UV ageing, the roughness parameters (Ra, Rz, Rt) increased several times. Long-lasting UV radiation influence on the surface of investigated acrylic-polyurethane coating systems contributed to their decorativeness loss. Microscopic examinations revealed colour change (yellowing) of blue pigment contained in polyurethane interlayer. Decline in coating gloss was also observed as the result of surface roughness increase.
Screening for Psychopathology Using the Three Factors Model of the Structure of Psychopathology: A Modified Form of GAIN Short Screener  [PDF]
Ibrahim A. Kira, Hanaa Shuwiekh, Justyna Kucharska
Psychology (PSYCH) , 2017, DOI: 10.4236/psych.2017.814152
Abstract: The goal of this paper is to develop a valid and reliable screening tool for mental health that is based on empirically and conceptually valid structure of psychopathology. Recently several studies of the structure of psychopathology found a general factor and three specific factors: internalizing, externalizing and thought disorder. We adapted the previously validated GAIN Short Screener to include the thought disorder that was not included in its original version and further developed its internalizing subscale. We conducted an exploratory and confirmatory factor analysis of the new adapted measure and produced 20 items screening tool that parsimoniously represents the three factors. The adapted screener and its subscales were found to have good reliability, stability, structural validity in two Egyptian and Polish samples. Additionally, all its subscales significantly correlated with different trauma types and with cumulative trauma, and negatively with self-esteem. The new adapted measure is the first that is based on robust scientific evidence of the structure of psychopathology and can be used in a broad scope of settings.
Assessment of the Erosive Wear Kinetics of Epoxy Coatings Modified with Nanofillers  [PDF]
Danuta Kotnarowska, Micha? Przerwa
Journal of Surface Engineered Materials and Advanced Technology (JSEMAT) , 2013, DOI: 10.4236/jsemat.2013.31A013

The paper presents results of investigation on the erosive wear kinetics of epoxy coatings modified with alumina or silica nanoparticles. Natural weathering caused a decrease of their erosive wear resistance. After a 3-year natural weathering, highest erosive wear resistance showed the epoxy coating modified with alumina nanoparticles.

The effect of the T6 heat treatment on the surface structure and oxide layer of an Al-Si coat
Barbara Kucharska,Anna Wrobel
Optica Applicata , 2009,
Abstract: The paper presents investigation results for an Al-Si protective coat deposited from a liquid phase on the ferritic steel surface. A coat of chemical composition of Al - 6.5 wt.% Si was subjected to a T6 heat treatment at temperatures of 420 °C (solutionizing), and 80 °C and 120 °C (ageing). The effect of soaking on the structure of the Al-Si coat was determined by microscopic examination. By XRD examination, the effect of soaking on the phase composition of the coat, while by GXRD examination, the oxide layer composition were determined. It was found that relaxation of the coat surface layer (a decay of the technological cold work effects) occurred, and a highly dispersed oxide layer Al2SiO5 formed as a result of the heat treatment.
Czynno tarczycy u dzieci chorych na wirusowe zapalenie opon mózgowo-rdzeniowych
Zofia Szychowska,Wioletta Kucharska
Pediatric Endocrinology, Diabetes and Metabolism , 1998,
Abstract: Zbadano czynno tarczycy u dzieci chorych na wirusowe zapalenie opon mózgowo-rdzeniowych, wywo ane wirusem winki lub enterowirusami. Mierzono st enia hormonu tyreotropowego (TSH), trójjodotyroniny (T3), wolnej trójjodotyroniny (FT3), tyroksyny (T4), wolnej tyroksyny (FT4) w surowicy lub osoczu krwi dwukrotnie w przebiegu choroby: przy przyj ciu do szpitala oraz w okresie zdrowienia, tj. mi dzy 10. a 14. dniem choroby. Hormony oznaczano metod radioimmunologiczn (RIA) b d fluoroimmunoenzymatyczn (ELFA). Wykazano obni enie st eń TSH, T3, FT3 oraz T4 (T4 - tylko w badaniu RIA) na pocz tku wirusowego zapalenia opon mózgowo-rdzeniowych, w porównaniu z grupami kontrolnymi, co wskazuje na obecno tzw. zespo u obni onego T3 u dzieci z wirusowym zapaleniem opon mózgowo-rdzeniowych. Zaburzenia te utrzymywa y si równie w okresie zdrowienia. Porównuj c funkcj tarczycy u dzieci chorych na ropne i wirusowe zapalenie opon mózgowo-rdzeniowych wykazano istotnie wi ksze obni enie hormonów tarczycy (szczególnie T3 i FT3) na pocz tku ropnego zapalenia opon mózgowo-rdzeniowych ni wirusowego. Przyczyny i znaczenie zespo u obni onego T3 w wirusowych zapaleniach opon mózgowo-rdzeniowych pozostaj nierozwi zanymi problemami.
Czynno tarczycy u dzieci chorych na ropne zapalenie opon mózgowo-rdzeniowych
Zofia Szychowska,Wioletta Kucharska
Pediatric Endocrinology, Diabetes and Metabolism , 1998,
Abstract: Bakteryjne zapalenie opon mózgowo-rdzeniowych jest z o onym procesem chorobowym, na którego wynik istotny wp yw ma odpowied organizmu, zarówno zapalna, jak i endokrynna, na zaka enie. W obecnej pracy zbadano czynno tarczycy u dzieci chorych na ropne zapalenie opon mózgowo-rdzeniowych, mierz c st enia hormonu tyreotropowego (TSH), trójjodotyroniny (T3), wolnej trójjodotyroniny (FT3), tyroksyny (T4), wolnej tyroksyny (FT4) w surowicy lub osoczu krwi trzykrotnie w przebiegu choroby: przy przyj ciu do szpitala, po 24-48 godzinach leczenia oraz w okresie zdrowienia, tj. mi dzy 10.-14. dniem od pocz tku choroby. Hormony oznaczano metod radioimmunologiczn (RIA) b d fluoroimmunoenzymatyczn (ELFA). Wykazano obni enie st eń wszystkich badanych hormonów na pocz tku ropnego zapalenia opon m.-rdz., w porównaniu z grupami kontrolnymi, co mo e odpowiada wariantowi eutyreotycznego zespo u chorobowego z niskim T4, który z regu y wyst puje u ci ko chorych. St enia badanych hormonów w okresie zdrowienia wzrasta y w porównaniu z pocz tkiem choroby, ale utrzymywa o si nadal obni enie T3, mierzonego obiema metodami, i TSH - mierzonego tylko metod RIA, w porównaniu z grupami kontrolnymi. Wykazano istotnie ni sze st enia T3 u dzieci, u których wyst pi y nast pstwa r.z.o., co wskazywa oby na niekorzystny wp yw zaburzeń czynno ci tarczycy w postaci zespo u obni onego T3 na wynik leczenia ropnego zapalenia opon m.-rdz. Wspomagaj ce leczenie przeciwzapalne deksametazonem, w dawce 0,4 mg/kg co 12 godzin przez 2 dni (pierwsza dawka podawana by a na 10-15 min przed zastosowaniem antybiotyku), prowadzi o do wi kszego obni enia st eń T3, FT3, T4 i FT4 w porównaniu z dzie mi nie leczonymi steroidem.
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