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Search Results: 1 - 10 of 32722 matches for " Daniel Degrandi "
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Analyses of murine GBP homology clusters based on in silico, in vitro and in vivo studies
Alexandra Kresse, Carolin Konermann, Daniel Degrandi, Cornelia Beuter-Gunia, Jan Wuerthner, Klaus Pfeffer, Sandra Beer
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-158
Abstract: The guanylate binding proteins (GBPs) were first described in 1979 when Gupta et al. identified a 67 kDa protein induced in human fibroblasts after interferon γ (IFNγ) stimulation [1]. Some years later, it was shown that two orthologous proteins are expressed in murine fibroblasts after stimulation with IFNγ [2]. Besides the strong inducer IFNγ, the GBPs can also be induced by type I interferons [2-6], tumor-necrosis-factor α (TNF-α), interleukin-1β (IL-1β), IL-1α [7,8], and TLR agonists [5].Human and murine GBPs possess the unique ability to bind to agarose-immobilized GMP (guanosine monophosphate), GDP (guanosine diphosphate), and GTP (guanosine triphosphate) with the same affinity, thereby differing from heterotrimeric or Ras-like GTP-binding proteins [2]. In addition, they hydrolyse GTP not only to GDP but also to GMP [9]. Further biochemical properties of the GBPs are the low binding affinity to nucleotides, their stability in the absence of guanine nucleotides and their high turnover GTPase activity [10]. Remarkably, the sequence of the common G4-motif N/TKxD is modified in the GBPs to the unique T(L/V)RD motif [10]. In the case of hGBP1 a nucleotide-dependent oligomerization and concentration-dependent GTPase activity has been observed [11]. These biochemical properties classified the GBPs as distantly related family members of the dynamin superfamily despite the lack of any sequence homology of the primary sequences [11,12]. The similarity to the dynamin family is further corroborated by the analysis of the crystal structure of hGBP1. It has an amino-terminal globular domain containing the GTP binding region and an elongated carboxy-terminal series of α-helices. The GBPs possess the common 'dynamin domain structure' with a GTPase domain (~300 residues), a 'middle' or 'assembly' domain (150–200 residues) and a GTPase effector domain (~100 residues) [11].Although, the GBPs have been discovered almost 30 years ago, only little is known about their biological fu
Host Cell Responses to Persistent Mycoplasmas - Different Stages in Infection of HeLa Cells with Mycoplasma hominis
Miriam Hopfe, René Deenen, Daniel Degrandi, Karl K?hrer, Birgit Henrich
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054219
Abstract: Mycoplasma hominis is a facultative human pathogen primarily associated with bacterial vaginosis and pelvic inflammatory disease, but it is also able to spread to other sites, leading to arthritis or, in neonates, meningitis. With a minimal set of 537 annotated genes, M. hominis is the second smallest self-replicating mycoplasma and thus an ideal model organism for studying the effects of an infectious agent on its host more closely. M. hominis adherence, colonisation and invasion of HeLa cells were characterised in a time-course study using scanning electron microscopy, confocal microscopy and microarray-based analysis of the HeLa cell transcriptome. At 4 h post infection, cytoadherence of M. hominis to the HeLa cell surface was accompanied by differential regulation of 723 host genes (>2 fold change in expression). Genes associated with immune responses and signal transduction pathways were mainly affected and components involved in cell-cycle regulation, growth and death were highly upregulated. At 48 h post infection, when mycoplasma invasion started, 1588 host genes were differentially expressed and expression of genes for lysosome-specific proteins associated with bacterial lysis was detected. In a chronically infected HeLa cell line (2 weeks), the proportion of intracellular mycoplasmas reached a maximum of 10% and M. hominis-filled protrusions of the host cell membrane were seen by confocal microscopy, suggesting exocytotic dissemination. Of the 1972 regulated host genes, components of the ECM-receptor interaction pathway and phagosome-related integrins were markedly increased. The immune response was quite different to that at the beginning of infection, with a prominent induction of IL1B gene expression, affecting pathways of MAPK signalling, and genes connected with cytokine-cytokine interactions and apoptosis. These data show for the first time the complex, time-dependent reaction of the host directed at mycoplasmal clearance and the counter measures of this pestering pathogen.
Guanylate-binding Protein 1 (Gbp1) Contributes to Cell-autonomous Immunity against Toxoplasma gondii
Elizabeth M. Selleck,Sarah J. Fentress,Wandy L. Beatty,Daniel Degrandi,Klaus Pfeffer,Herbert W. Virgin IV,John D. MacMicking,L. David Sibley
PLOS Pathogens , 2013, DOI: 10.1371/journal.ppat.1003320
Abstract: IFN-γ activates cells to restrict intracellular pathogens by upregulating cellular effectors including the p65 family of guanylate-binding proteins (GBPs). Here we test the role of Gbp1 in the IFN-γ-dependent control of T. gondii in the mouse model. Virulent strains of T. gondii avoided recruitment of Gbp1 to the parasitophorous vacuole in a strain-dependent manner that was mediated by the parasite virulence factors ROP18, an active serine/threonine kinase, and the pseudokinase ROP5. Increased recruitment of Gbp1 to Δrop18 or Δrop5 parasites was associated with clearance in IFN-γ-activated macrophages in vitro, a process dependent on the autophagy protein Atg5. The increased susceptibility of Δrop18 mutants in IFN-γ-activated macrophages was reverted in Gbp1?/? cells, and decreased virulence of this mutant was compensated in Gbp1?/? mice, which were also more susceptible to challenge with type II strain parasites of intermediate virulence. These findings demonstrate that Gbp1 plays an important role in the IFN-γ-dependent, cell-autonomous control of toxoplasmosis and predict a broader role for this protein in host defense.
Exposi??o ocupacional a resíduos de gases anestésicos
Oliveira, Carlos Rogério Degrandi;
Revista Brasileira de Anestesiologia , 2009, DOI: 10.1590/S0034-70942009000100014
Abstract: background and objectives: although the absence of negative effects of prolonged exposure to anesthetic gases residue has been reported, controversies on the subject still linger. contradictory data on the variability in individual response to different agents can be found in the literature. the objective of this report was to present a review of occupational exposure to anesthetic gases residue. contents: the results of the main articles on the subject, as well as the causes of contamination of the surgical environment, ventilation, exhaust system, monitoring, and dosage of anesthetic gases residues are discussed. recommendations to minimize the supposed effects of inhalational agents are emphasized. conclusions: even in task-forces studies of renowned international regulating institutions, there are some controversies on the risks of occupational exposure to anesthetic gases residue. minimal values for occupational exposure are stipulated, but acknowledging the lack of epidemiological evidence of any type of damage caused by said exposure in places where standard measures of ventilation and exhaust systems and the use of anesthetic equipment are observed. in our country, most of the time those measures are not implemented and, when they are, they are not supervised properly. besides, differences in techniques and working conditions have to be considered. taking into consideration the multifactorial nature of the exposure of health care professionals, measures should be undertaken to minimize occupational exposure to agents with known or probable toxic potential. the demand for better equipped operating rooms, with adequate ventilation and exhaust systems as well as their maintenance should be stimulated.
Espondilite anquilosante e anestesia
Oliveira, Carlos Rogério Degrandi;
Revista Brasileira de Anestesiologia , 2007, DOI: 10.1590/S0034-70942007000200011
Abstract: background and objectives: ankylosing spondylitis (as) is a chronic inflammatory disease of the joints, included in the group of seronegative spondyloarthropathies. its main characteristic is the fusion of the bones in the spine, which causes loss of flexibility of the back and neck. other large articulations and connective tissues can be affected by the inflammatory process. it affects mainly men between the ages of 20 and 40; it is rare after the age of 50. women represent a minority of patients. there is little information about as in the anesthetic literature. the objective of this article was to review the characteristics of as pertaining anesthesiology for an adequate perioperative handling. contents: the clinical characteristics of ankylosing spondylitis pertaining to the anesthetic conduct are reviewed. conclusions: patients with chronic diseases of the spine represent specific challenges to the anesthesiologist. handling of the airways and the access to the neuroaxis can be difficult. most anesthesiologists prefer to use general anesthesia, avoiding the neuroaxis, in those patients, despite the presence of difficult airways. the degree of spine involvement will determine how difficult the tracheal intubation might be. special care should be taken to avoid excessive manipulation of the neck, which could cause trauma to the spinal cord.
Anestesia em paciente com síndrome de Rubinstein-Taybi: relato de caso
Oliveira, Carlos Rogério Degrandi;Elias, Luciana;
Revista Brasileira de Anestesiologia , 2005, DOI: 10.1590/S0034-70942005000500010
Abstract: background and objectives: rubinstein-taybi syndrome (rts) is a genetic disease caused by mutation or deletion of chromosome 16, and characterized by growth and mental retardation, clubbing thumbs and toes and craniofacial abnormalities. there is little information about rts in the anesthetic literature. this report aimed at describing the anesthetic management of a patient with rubinstein-taybi syndrome submitted to dentistry surgery and at discussing aspects of this syndrome that may be relevant to anesthesia. case report: male patient, 9-year-old, 28 kg, to be submitted to general anesthesia for extraction of abnormally positioned teeth. patient presented with typical rts characteristics (growth and mental retardation, clubbing thumbs and toes and craniofacial abnormalities). anesthesia was induced and initially maintained with sevoflurane under facial mask; after tracheal intubation it was maintained under manually controlled ventilation with bain system. there have been no complications and procedure was performed in outpatient regimen. conclusions: the importance of thorough preanesthetic evaluation is emphasized due to possible associated malformations, including heart diseases. in this particular case, there was no heart disease, which is reported to be present in one-third of cases. attention to difficult airway maintenance is recommended.
Simone Marafiga Degrandi,Adriano Severo Figueiró
Revista Brasileira de Ecoturismo , 2011,
Abstract: Atualmente tem se intensificado a procura/visita a ambientes naturais onde os aspectos geológico-geomorfológicos se destacam como os principais atrativos turísticos. Entre as principais alternativas, ligadas ao desenvolvimento do turismo sobre bases sustentáveis, destaca-se o papel desempenhado pelo Ecoturismo e pelo Geoturismo no processo de interpreta o da paisagem e da Educa o Ambiental, além do aproveitamento do patrim nio natural e cultural das comunidades receptoras, como uma das principais ferramentas de desenvolvimento territorial endógeno. A partir dessa perspectiva, o enfoque central do presente trabalho foi identificar as principais geoformas (patrim nio geológico-geomorfológico) existentes no Município de Ca apava do Sul/RS, com o intuito de discutir o processo de geoconserva o através da valoriza o da geodiversidade e do potencial natural da paisagem em estudo, para o desenvolvimento de atividades de Geoturismo e Ecoturismo. A abordagem dessa temática deve-se a necessidade de uma ampla divulga o e reflex o sobre a importancia da conserva o dos elementos abióticos que comp em paisagens de excepcional beleza cênica e/ou importancia geocientífica no RS e, em especial, no Município de Ca apava do Sul. A metodologia adotada envolveu o levantamento de dados sobre a configura o da paisagem da área em estudo, através de pesquisa bibliográfica, análise do mapeamento geológico, geomorfológico e de cartas topográficas nas escalas 1:250.000 e 1:50.000. A partir da análise das cartas topográficas, foi possível identificar a distribui o espacial das principais eleva es topográficas e afloramentos rochosos do município. Esta etapa, também foi complementada através da observa o destas formas de relevo através do software Google Earth. Por fim, foram realizados trabalhos de campo, com intuito de identificar in loco, as geoformas inventariadas através da pesquisa, formando um banco de dados referente a cada uma das estruturas. A etapa final compreendeu a interpreta o dos dados obtidos e a identifica o das principais geoformas de Ca apava do Sul, que possam vir a ser aproveitadas para o desenvolvimento de atividades de Geoturismo e Ecoturismo. As principais geoformas inventariadas foram: Guaritas (várias geoformas), Minas do Camaqu , Gruta da Varzinha, Toca das Carretas, Pedra do Segredo (várias geoformas), Pedra Furada, Passo do Pessegueiro, Morro da Angélica, Camping Galp o de Pedra (várias geoformas), entre outras. Ca apava do Sul se destaca no cenário eco-geo-turístico por apresentar um singular patrim nio geológico-geomorfológico, acrescido d
Patrim nio Natural e Geoconserva o: a geodiversidade do município gaúcho de Ca apava do Sul
Simone Marafiga Degrandi,Adriano Severo Figueiró
Revista Brasileira de Ecoturismo , 2012,
Abstract: O presente trabalho possui a finalidade de discutir o processo de geoconserva o através da valoriza o da geodiversidade do município gaúcho de Ca apava do Sul para o desenvolvimento de atividades de Ecoturismo e Geoturismo, enfatizando os aspectos macroestruturais do patrim nio natural (fatores geológico-geomorfológicos). Para atingir tal objetivo, foi realizado o levantamento das principais geoformas e afloramentos rochosos com potencialidades interpretativas para o desenvolvimento do turismo no município. Através da abordagem desta temática busca-se discutir também a singularidade da paisagem em estudo e a importancia da conserva o de seu patrim nio natural, considerando-a como um local para a aplica o dos princípios relativos à geoconserva o. A beleza cênica da configura o da paisagem de Ca apava do Sul, inserida no contexto geológico do Escudo Cristalino Sul rio-grandense e da Bacia Sedimentar do Camaqu , é marcada pela ocorrência de diversos afloramentos rochosos (principalmente arenitos paleozóicos) dispostos em forma de complexos e também de morros isolados, que comp em um patrim nio natural de ordem abiótica a ser preservado. A ocorrência de estruturas rochosas de formatos curiosos em meio à vegeta o típica do Pampa gaúcho agu a o espírito aventureiro de turistas que procuram roteiros alternativos e um maior contato com a natureza. A metodologia adotada para a constru o dessa análise partiu do levantamento de dados sobre a configura o da paisagem em estudo, associada às análises cartográficas e trabalhos de campo que deram suporte á pesquisa. A partir da geodiversidade encontrada no Município de Ca apava do Sul com possibilidades interpretativas para o desenvolvimento do Ecoturismo e/ou Geoturismo destacam-se: as Guaritas, a Vila Minas do Camaqu , as geoformas da Vila do Segredo, a Gruta da Varzinha, a Toca das Carretas, a Cascata do Salso, entre outras. O levantamento das geoformas presentes em um determinado território pode auxiliar, entre outros objetivos, na conserva o de paisagens/sítios em situa o vulnerável, a valoriza o do patrim nio natural e histórico-cultural dentro do contexto do turismo sustentável e a complementa o da renda das comunidades que residem nestes locais de rara beleza cênica e importancia científica. ABSTRACT The present study has the purpose of discussing the process of geoconservation through the valorization of the geodiversity the Ca apava do Sul city, Rio Grande do Sul, for the development of ecotourism and geotourism activities, emphasizing macro-natural aspects of the natural heritage (geological and
Desenvolvimento regional: a diversidade regional como potencialidade
Virginia Elisabeta Etges,José Odim Degrandi
Revista Brasileira de Desenvolvimento Regional , 2013, DOI: 10.7867/2317-5443.2013v1n1p085-094
Abstract: Throughout the second half of the twentieth century the debate on regional development in Brazil received special attention, especially among economists and geographers. Two main approaches stand out in the debate: one that understands the promotion of regional development from the reduction / elimination of regional inequalities, and the other proposes the understanding of regional diversity and potentiality for developing regions. The first was and still remains hegemonic, being evidenced both in scientific papers and in documents guiding public policies in the area. The latter is evidenced most clearly from the 1990s on, a period when the concept of region is resumed under a new approach. This paper aims to clarify the meaning of each of these concepts in order to contribute to the qualification of the discussion on regional development.
Exposi??o ocupacional a polui??o sonora em anestesiologia
Oliveira, Carlos Rogério Degrandi;Arenas, Gilberto Walter Nogueira;
Revista Brasileira de Anestesiologia , 2012, DOI: 10.1590/S0034-70942012000200011
Abstract: background and objectives: the harmful effects of workplace noise pollution are well known and described in the literature. the effects of prolonged exposure to noise in areas demanding high level of concentration, such as operating rooms, depend on the variability of individual responses and intensity of different generation sources. the aim of this paper is to present a review of occupational exposure to noise in anesthesiology. content: the results of the main articles in literature on the subject are discussed, concerning the sources of noise pollution and its effects on workers, particularly the anesthesiologist. emphasis is given to legislation and recommendations to minimize the effects caused by noise.
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