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Search Results: 1 - 10 of 24020 matches for " Daiming Jiang "
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Comparative Transcriptomes Profiling of Photoperiod-sensitive Male Sterile Rice Nongken 58S During the Male Sterility Transition between Short-day and Long-day
Wei Wang, Zhenwei Liu, Zhibin Guo, Gaoyuan Song, Qin Cheng, Daiming Jiang, Yingguo Zhu, Daichang Yang
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-462
Abstract: To investigate the transcriptome during the male sterility transition in PGMS rice, the transcriptome of Nongken 58S under short-day (SD) and long-day (LD) at the glume primordium differentiation and pistil/stamen primordium forming stages was compared. Seventy-three and 128 differentially expressed genes (DEGs) were identified at the glume primordium differentiation and pistil/stamen primordium forming stages, respectively. Five and 22 genes were markedly up-regulated (≥ 5-fold), and two and five genes were considerably down-regulated (≥ 5-fold) under SD during the male sterility transition. Gene ontology annotation and pathway analysis revealed that four biological processes and the circadian rhythms and the flowering pathways coordinately regulated the male sterility transition. Further quantitative PCR analysis demonstrated that the circadian rhythms of OsPRR1, OsPRR37, OsGI, Hd1, OsLHY and OsDof in leaves were obviously different between Nongken 58S and Nongken 58 under LD conditions. Moreover, both OsPRR37 and Hd1 in the inflorescence displayed differences between Nongken 58S and Nongken 58 under both LD and SD conditions.The results presented here indicate that the transcriptome in Nongken 58S was significantly suppressed under LD conditions. Among these DEGs, the circadian rhythm and the flowering pathway were involved in the male sterility transition. Furthermore, these pathways were coordinately involved in the male sterility transition in PGMS rice.Photoperiod-sensitive genic male sterile (PGMS) rice, Nongken 58S, was discovered as a spontaneous mutant in the japonica rice cultivar Nongken 58 (Oryza sativa ssp. japonica) grown in Hubei Province, China in 1973 [1]. It has since been used for production of hybrid rice, and during the past two decades, great achievements have been made in improving rice yields in China using two-line hybrid rice. Several important features of Nongken 58S have been characterised. Its fertility is highly regulated by day lengt
Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
Na An,Jiquan Ou,Daiming Jiang,Liping Zhang,Jingru Liu,Kai Fu,Ying Dai,Daichang Yang
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14023556
Abstract: Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains ( Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins.
Ectopic Expression of MiR-125a Inhibits the Proliferation and Metastasis of Hepatocellular Carcinoma by Targeting MMP11 and VEGF
Qian Bi, Shanhong Tang, Lin Xia, Rui Du, Rui Fan, Liucun Gao, Jiang Jin, Shuhui Liang, Zheng Chen, Guanghui Xu, Yongzhan Nie, Kaichun Wu, Jie Liu, Yongquan Shi, Jie Ding, Daiming Fan
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040169
Abstract: Background Studies have been shown that miR-125a plays an important role in carcinogenesis, however, the role of miR-125a in hepatocellular carcinoma (HCC) remains elusive. Methodology/Principal Real time-PCR (qRT-PCR) was performed to test the significance of miR-125a in HCC. Ectopic expression of miR-125a was used to test the influences of miR-125a on proliferation and metastasis of HCC cells in vitro and in vivo. Predicted target genes of miR-125a were determined by dual-luciferase reporting, qRT-PCR, and western blot (WB) analyses. Then immunohistochemical staining (IHC) was used to detect the expression of target genes, and the correlations and prognostic values of miR-125a and its target genes were also investigated. Conclusions/Significance Decreased miR-125a was observed in both HCC tissues and cell lines, and associated with patients’ aggressive pathologic features. Up-regulating miR-125a significantly inhibited the malignant phenotypes by repressing the expression of matrix metalloproteinase 11 (MMP11) and vascular endothelial growth factor A (VEGF-A) both in vitro and in vivo. Furthermore, miR-125a expression was inversely correlated with both MMP11 and VEGF-A expression in HCC tissues. Inhibiting miR-125a could increase both MMP11 and VEGF-A expression, and RNA interference targeting MMP11 or VEGF-A mRNA could rescue the loss of miR-125a functions. MiR-125a inhibits the proliferation and metastasis of HCC by targeting MMP11 and VEGF-A. Up-regulation of miR-125a might be a promising approach and a prognostic marker for HCC.
An Improved Electric Model with Online Parameters Correction for Large Li-Ion Battery Packs
Daiming Yang,Chao Lu,Guoguang Qi
International Journal of Computer and Electrical Engineering , 2013, DOI: 10.7763/ijcee.2013.v5.725
Abstract: Lithium-ion battery packs based battery energy storage system (BESS) has proved its high potential in energy storage. In studying the BESS, A precise battery model is critical for implementing the system simulation, estimating the SOC, SOH of the battery, as well as optimizing the design. An electric model which can reflect the hysteresis effect and dynamic characteristic is adopted in this paper, and based on that, we proposed a novel method for battery model identification and on-line correction of model parameters by extended Kalman filter (EKF). Thus the parameters of battery model can be regulated in the progress of battery charge and discharge. The method is proved to be efficient and accurate by comparing the simulation result with the experiment data.
Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres
Qing Ji, Xinbao Hao, Yang Meng, Min Zhang, Jeffrey DeSano, Daiming Fan, Liang Xu
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-266
Abstract: Human gastric cancer cells were transfected with miR-34 mimics or infected with the lentiviral miR-34-MIF expression system, and validated by miR-34 reporter assay using Bcl-2 3'UTR reporter. Potential target gene expression was assessed by Western blot for proteins, and by quantitative real-time RT-PCR for mRNAs. The effects of miR-34 restoration were assessed by cell growth assay, cell cycle analysis, caspase-3 activation, and cytotoxicity assay, as well as by tumorsphere formation and growth.Human gastric cancer Kato III cells with miR-34 restoration reduced the expression of target genes Bcl-2, Notch, and HMGA2. Bcl-2 3'UTR reporter assay showed that the transfected miR-34s were functional and confirmed that Bcl-2 is a direct target of miR-34. Restoration of miR-34 chemosensitized Kato III cells with a high level of Bcl-2, but not MKN-45 cells with a low level of Bcl-2. miR-34 impaired cell growth, accumulated the cells in G1 phase, increased caspase-3 activation, and, more significantly, inhibited tumorsphere formation and growth.Our results demonstrate that in p53-deficient human gastric cancer cells, restoration of functional miR-34 inhibits cell growth and induces chemosensitization and apoptosis, indicating that miR-34 may restore p53 function. Restoration of miR-34 inhibits tumorsphere formation and growth, which is reported to be correlated to the self-renewal of cancer stem cells. The mechanism of miR-34-mediated suppression of self-renewal appears to be related to the direct modulation of downstream targets Bcl-2, Notch, and HMGA2, indicating that miR-34 may be involved in gastric cancer stem cell self-renewal/differentiation decision-making. Our study suggests that restoration of the tumor suppressor miR-34 may provide a novel molecular therapy for p53-mutant gastric cancer.MicroRNAs (miRNAs) are a conserved class of non-coding 20–22 nt small RNAs that regulate gene expression by binding to mRNA, leading to mRNA degradation or inhibition [1]. miRNAs re
The function and mechanism of COX-2 in angiogenesis of gastric cancer cells
Liping Yao, Fei Liu, Liu Hong, Li Sun, Shuhui Liang, Kaichun Wu, Daiming Fan
Journal of Experimental & Clinical Cancer Research , 2011, DOI: 10.1186/1756-9966-30-13
Abstract: The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2.Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN.COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.Gastric cancer is the second leading cause of cancer associated death in the world, particularly in Asian countries. The treatment outcome of this common malignancy is still not satisfactory and various chemotherapeutic attempts in an adjuvant setting have failed to improve the survival rate in gastric cancer. Recently, angiogenesis has been found related to hematogenous recurrence and poor prognosis in gastric cancer [1]. Angiogenesis is the growth of new vessels from existing vasculature. A balance of angiogenic and angiostatic growth factors tightly controls physiological angiogenesis. Tipping of this balance towards a pro-angiogenic environment is termed the 'angiogenic switch' and occurs in situations such as tissue hypoxia, inflammation or neoplasia [2].COX-2, a COX isoenzyme catalyzing the production of prostaglandins, has been observed in most gastric cancer tissues compared with the accompanying normal mucosa. Studies in different cancers have suggested a relationship between COX-2 and increased pro-angiogenic growth factors, in particular VEGF [3]. COX-2 is thought to promote angioge
Dynamic expression of CEACAM7 in precursor lesions of gastric carcinoma and its prognostic value in combination with CEA
Jinfeng Zhou, Liyun Zhang, Yong Gu, Kai Li, Yongzhan Nie, Daiming Fan, Yichao Feng
World Journal of Surgical Oncology , 2011, DOI: 10.1186/1477-7819-9-172
Abstract: CEACAM7 and CEA expression was detected by immunohistochemistry in consecutive sections of 345 subjects with gastric carcinoma and precancerous lesions. Laser confocal analysis was performed to determine CEACAM7 and CEA localization. Correlation between CEACAM7 and CEA expression with clinicopathological parameters was statistically analyzed.CEACAM7 expression correlated with pathologic grading (P = 0.006), Lauren's classification (P = 0.023), and CEA expression (Spearman R = 0.605, P < 0.001) in gastric carcinoma. CEACAM7 co-localized with CEA predominantly in the cytoplasmic membrane of cancerous cells. CEA expression was correlated with lymph node metastasis (P = 0.031). CEACAM7 and CEA expression increased progressively from precursor lesions to gastric carcinomas. A combination of CEACAM7 and CEA expression was determined to be an independent predictor for patients with gastric carcinoma by multivariate analysis (P = 0.001).CEACAM7 expression correlates with tumor differentiation and CEA expression in gastric carcinoma. CEACAM7 and CEA expression may synergistically promote gastric carcinogenesis. Combined CEACAM7 and CEA expression analysis can be a useful postoperative predictor for patients with gastric carcinoma.In-depth knowledge of the genotypic and phenotypic characteristics of gastric carcinoma and precancerous lesions is extremely important for preventing their development, curtailing their progression to a higher grade tumor once they develop, and providing prognostic information. Gastric carcinomas have generally been divided into intestinal-type and diffuse-type by Lauren [1]. The intestinal-type cancer was thought to display a predominantly intestinal phenotype because it is preceded by a precancerous stage characterized by the sequential steps of atrophic gastritis, intestinal metaplasia, intraepithelial neoplasia (GIN), and intramucosal carcinoma [2,3]. GIN is recognized as an important precancerous lesion, and is categorized as either high- or l
A practical pilot-based channel estimation method for space-time coded ofdm system

Qu Daiming,Zhu Guangxi,Chen Zhonglin,

电子与信息学报 , 2003,
Abstract: This paper deals with pilot-based channel estimation of space-time coded OFDM system in frequency selective channels. DFT-based LS and LMMSE method are studied. A practical channel estimation method is proposed. In proposed method the complexity of LS and LMMSE methods is greatly reduced by optimally designing the pilot symbols. And the performance demonstrated by simulation is close to idea channel information.
Find Duplicates among the PubMed, EMBASE, and Cochrane Library Databases in Systematic Review
Xingshun Qi, Man Yang, Weirong Ren, Jia Jia, Juan Wang, Guohong Han, Daiming Fan
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071838
Abstract: Background Finding duplicates is an important phase of systematic review. However, no consensus regarding the methods to find duplicates has been provided. This study aims to describe a pragmatic strategy of combining auto- and hand-searching duplicates in systematic review and to evaluate the prevalence and characteristics of duplicates. Methods and Findings Literatures regarding portal vein thrombosis (PVT) and Budd-Chiari syndrome (BCS) were searched by the PubMed, EMBASE, and Cochrane library databases. Duplicates included one index paper and one or more redundant papers. They were divided into type-I (duplicates among different databases) and type-II (duplicate publications in different journals/issues) duplicates. For type-I duplicates, reference items were further compared between index and redundant papers. Of 10936 papers regarding PVT, 2399 and 1307 were identified as auto- and hand-searched duplicates, respectively. The prevalence of auto- and hand-searched redundant papers was 11.0% (1201/10936) and 6.1% (665/10936), respectively. They included 3431 type-I and 275 type-II duplicates. Of 11403 papers regarding BCS, 3275 and 2064 were identified as auto- and hand-searched duplicates, respectively. The prevalence of auto- and hand-searched redundant papers was 14.4% (1640/11403) and 9.1% (1039/11403), respectively. They included 5053 type-I and 286 type-II duplicates. Most of type-I duplicates were identified by auto-searching method (69.5%, 2385/3431 in PVT literatures; 64.6%, 3263/5053 in BCS literatures). Nearly all type-II duplicates were identified by hand-searching method (94.9%, 261/275 in PVT literatures; 95.8%, 274/286 in BCS literatures). Compared with those identified by auto-searching method, type-I duplicates identified by hand-searching method had a significantly higher prevalence of wrong items (47/2385 versus 498/1046, p<0.0001 in PVT literatures; 30/3263 versus 778/1790, p<0.0001 in BCS literatures). Most of wrong items originated from EMBASE database. Conclusion Given the inadequacy of a single strategy of auto-searching method, a combined strategy of auto- and hand-searching methods should be employed to find duplicates in systematic review.
Child-Na Score: A Predictive Model for Survival in Cirrhotic Patients with Symptomatic Portal Hypertension Treated with TIPS
Hui Chen, Ming Bai, Xingshun Qi, Lei Liu, Chuangye He, Zhanxin Yin, Daiming Fan, Guohong Han
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0079637
Abstract: Background and Aim Several models have been developed to predict survival in patients with cirrhosis undergoing TIPS; however, few of these models have gained widespread acceptance, especially in the era of covered stents. The aim of this study was to establish an evidence-based model for predicting survival after TIPS procedures. Methods A total of 210 patients with cirrhosis treated with TIPS were considered in the study. We comprehensively investigated factors associated with one-year survival and developed a new predictive model using the Cox regression model. Results In the multivariate analysis, the Child-Pugh score and serum sodium levels were independent predictors of one-year survival. A new score incorporating serum sodium into the Child-Pugh score was developed: Child-Na score. We compared the predictive accuracy of Child-Na score with that of other scores; only the Child-Na and MELD-Na scores had adequate predictive ability in patients with serum Na levels <138 mmol/L. The best Child-Na cut-off score (15.5) differentiated two groups of patients with distinct prognoses (one-year cumulative survival rates of 80.6% and 45.5%); this finding was confirmed in a validation cohort (n = 86). In a subgroup analysis stratifying patients by indication for TIPS, the Child-Na score distinguished patients with different prognoses. Conclusions Patients with variceal bleeding and a Child-Na score ≤15 had a better prognosis than patients with a score ≥16. Patients with refractory ascites and a Child-Na score ≥16 had a high risk of death after the TIPS procedures; caution should be used when treating these patients with TIPS.
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