OALib Journal期刊

ISSN: 2333-9721




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匹配条件: “Daami-Remadi M.” ,找到相关结果约400196条。
Présence en Tunisie d'isolats de Fusarium sambucinum résistants aux benzimidazoles : développement in vitro et agressivité sur tubercules de pomme de terre
Daami-Remadi M.,El Mahjoub M.
Biotechnologie, Agronomie, Société et Environnement , 2006,
Abstract: Presence in Tunisia of Fusarium sambucinum isolates resistant to benzimidazoles: in vitro growth and aggressiveness on potato tubers. The behaviour of 55 isolates of Fusarium spp. causing dry rot of the potato tubers, is studied against some enzimidazoles fungicides. Tunisian isolates of F. solani (12), F. oxysporum (23) and F. graminearum (10) are sensitive in vitro to carbendazime and benomyl at 5 mg.l-1. Their interaction with thiophanate-methyl is different; a complete inhibition of their mycelial growth is observed at doses higher than 500 mg.l-1. Tunisian isolates of F. sambucinum collected during 2002, 2003 and 2004 are resistant to these benzimidazoles showing existence of a cross-resistance. In fact, these isolates tolerated carbendazime (and benomyl) at 200 mg.l-1 and thiophanate-methyl at 1000 mg.l-1. This is the first study in Tunisia indicating emergence of this type of F. sambucinum resistance. Control isolates of F. sambucinum and those treated with carbendazime at 100 mg.l-1 showed a similar aggressiveness on potato tubers of the Spunta cultivar.
Effets de certains fongicides de synthèse et biologiques sur la croissance mycélienne et l'agressivité de Fusarium oxysporum f.sp. radicis-lycopersici
Hibar, K.,Daami-Remadi, M.,El Mahjoub, M.
Tropicultura , 2007,
Abstract: Effect of some Chemical and Biological Fungicides on Mycelial Growth and Disease Severity of Fusarium oxysporum f.sp. radicis-lycopersici. Fusarium is among the most aggressive telluric fungi causing wilt and root rots in several vegetable crops. A new disease caused by Fusarium oxysporum f. sp. radicis-lycopersici was recorded in southern Tunisia in the "Cinquième Saison" farm, situated in Hammet Gabès during 2000-2001 crop season. It caused death of up to 90% of tomato plants in some greenhouses. In the present research, the effect of 3 chemical and 4 biological fungicides was tested in vitro on mycelial growth and in vivo on disease severity of F. oxysporum f. sp. radicis-lycopersici. Hymexazol, benomyl and manebe were used as chemical fungicides whereas four biological products, i.e. two based on Trichoderma harzianum, one on Bacillus subtilis and another one on Bacillus thuringiensis were also tested. Among the chemical fungicides which were used, hymexazol and benomyl were the most effective in vitro and inhibited mycelial growth up to 80%. However, in vivo assays showed that only hymexazol was effective with a reduction in disease incidence of about 76%. In vitro, the product based on B. thuringiensis entailed a mycelial growth inhibition of less than 20%. This value is more than 75% higher than what was obtained through the other biological fungicides based on either T. harzianum or in B. subtilis. The efficacy of the latter 2 biological fungicides was more important in vivo assays using inoculated tomato plants. Indeed, by the use of the product based on B. subtilis, the reduction of disease incidence exceeded 95%. These results show that some biological fungicides can be used in controlling Fusarium crown and root rot of tomato.
In vitro and in vivo Interaction of Four Fungicides with the Fusarium Species Complex Causing Tuber Dry Rot in Tunisia
M. Daami-Remadi,H. Jabnoun-Khiareddine,F. Ayed,M. El Mahjoub
Plant Pathology Journal , 2006,
Abstract: Several fungicides were tested against some isolates belonging to four Fusarium species causing potato tuber dry rot in Tunisia. Incorporated into the culture media PDA, the tested fungicides significantly inhibited the mycelial growth, observed after incubation at 25°C for 4 days, of all Fusarium isolates including those of F. sambucinum resistant to benzimidazoles. A significant interaction (p =< 0.05) was observed between both fixed factors where inhibition percentage varied depending on tested pathogens and fungicides. Applied on potato tubers (tuber immersion for 10 min) prior inoculation, certain tested fungicides such as azoxystrobin and fludioxonil significantly reduced by more than 50%, comparatively to the untreated controls, the development of dry rot occasioned by F. graminearum and F. sambucinum observed after 21 days of incubation at 25-27°C. A significant interaction (p =< 0.05) was noted between the treatments and the Fusarium species traduced by a variable inhibition percentage depending on tested pathogen and fungicides.
Effet inhibiteur in vitro et in vivo du Trichoderma harzianum sur Fusarium oxysporum f. sp. Radicis-lycopersici
Hibar K.,,Daami-Remadi M.,Khiareddine H.,El Mahjoub M.
Biotechnologie, Agronomie, Société et Environnement , 2005,
Abstract: In vitro and in vivo antagonistic effect of Trichoderma harzianum against Fusarium oxysporum f. sp. radicis-lycopersici. Tests of direct confrontation, on PDA medium or remote confrontation, between Fusarium oxysporum f. sp. radicislycopersici and Trichoderma harzianum, revealed that the latest has inhibited mycelial growth of the pathogen by more than 65% compared to the control and this after an incubation period of about four days at 25°C. Moreover, beyond this period and after six days, T. harzianum invades and also sporulates on F. oxysporum f. sp. radicis-lycopersici colonies revealing its high myco-parasitism. Some interesting results have also been obtained in vivo: indeed, transplantation of tomato seedlings in a mixture of perlite inoculated by F. oxysporum f. sp. radicis-lycopersici and T. harzianum reduced Fusarium crown and root rot incidence compared to seedlings planted in perlite only inoculated by the pathogen. Even better, seedlings planted in perlite containing the pathogen and the studied antagonist present a better vegetative growth and a vigorous root system compared to those of the healthy control (not inoculated and untreated).
Effect of Potato Cultivars on Incidence of Fusarium oxysporum f.sp. tuberosi and its Transmission to Progeny Tubers
F. Ayed,M. Daami-Remadi,H. Jabnoun-Khiareddine,M. El Mahjoub
Journal of Agronomy , 2006,
Abstract: Behaviour of some potato cultivars to Fusarium wilt caused by Fusarium oxysporum f.sp. tuberosi was studied. Plants were inoculated after two weeks by irrigation with a conidial suspension (107 spores mL-1). Variations of Fusarium wilt incidence were observed on potato 14 cultivars. Cv. Baraka was the most tolerant, Lyra and Platina were the most susceptible and Asterix, Alaska, Safrane and Timate were found to be intermediate with various degrees of response. Pathogen transmission to progeny tubers was assessed by planting daughter tubers coming from inoculated and non inoculated plants. Differences in incidence of cultivar susceptibility were significant. Disease incidence of Latona and Timate was the most and the least important, respectively.
Effect of Temperature on Aggressivity of Tunisian Fusarium species Causing Potato (Solanum tuberosum L.) Tuber Dry Rot
M. Daami-Remadi,H. Jabnoun-Khiareddine,F. Ayed,M. El Mahjoub
Journal of Agronomy , 2006,
Abstract: Mycelial growth on PDA of Fusarium graminearum, F. oxysporum f. sp. tuberosi, F. sambucinum and F. solani, causal agents of potato tuber dry rot, depends upon temperatures ranging from 5 to 40°C. An interaction between pathogens tested and temperatures used for culture incubation was observed (at p=<0.05). These Fusarium species showed in vivo both thermal pics of aggressivity: a first one at low temperatures (10-15°C) and a second at higher temperatures (30-35°C). Nevertheless, at temperatures less than 25°C, F. sambucinum and F. graminearum were the most aggressive, while F. solani was the most aggressive at temperatures superior to 30°C.
Effet des extraits de compost sur la croissance mycélienne et l'agressivité du Fusarium oxysporum f. sp. radicis-lycopersici
Hibar K.,Daami-Remadi M.,Jabnoun-Khiareddine H.,El Akram Zna?di I.
Biotechnologie, Agronomie, Société et Environnement , 2006,
Abstract: Effect of compost tea on mycelial growth and disease severity of Fusarium oxysporum f. sp. radicis-lycopersici. Simultaneous addition, on culture media, of Fusarium oxysporum f. sp. radicis-lycopersici and compost teas revealed that the latest induced the inhibition of the mycelial growth of the pathogen. This inhibition, noted after an incubation period of about six days at 25°C, was more important when compost teas were enriched in PDB (Potato Dextrose Broth), where it may reach 70% compared to the control. Transplantation of tomato seedlings, previously inoculated by a conidial suspension of F. oxysporum f. sp. radicis-lycopersici, in a container media (peat, perlite or the mixture of the two substrates) treated by compost teas has signifi cantly reduced Fusarium crown and root rot incidence compared to inoculated and untreated control seedlings. Disease incidence is more reduced when tomato inoculated plants are transplanted in peat treated by compost teas; indeed, these plants don t show any wilting and present a vigorous root system and a better vegetative growth.
In vitro and in vivo Evaluation of Individually Compost Fungi for Potato Fusarium Dry Rot Biocontrol
M. Daami-Remadi,H. Jabnoun-Khiareddine,F. Ayed,K. Hibar
Journal of Biological Sciences , 2006,
Abstract: Eight thermo-resistant fungi isolated from compost extracts are tested, for the first time in Tunisia, for their antagonistic activity, in vitro by dual culture with Fusarium spp. on PDA and in vivo by wound treatment prior to tuber inoculation. In vitro experiments showed that all tested compost fungi significantly inhibited mycelial growth of F. graminearum, F. sambucinum, F. solani and F. oxysporum f.sp. tuberosi, observed after incubation at 25°C during 6 days, revealing a higher competition. Light microscopic studies of tested pathogen mycelium, removed from the confrontation zone of both microorganisms, showed multiple mechanisms of action including mycoparasitism, lysis, early formation of chlamydospores and induction of mycelial cords via anatomosis between mycelial filaments. Tuber (cv. Spunta) treatment, 24 h before their inoculation by individually thermoresistant fungi suspensions, reduced development of dry rot after incubation for 21 days at 25-27°C, comparatively to the untreated control and this for the majority of the Fusarium complex tested.
Effects of Some Bacillus sp. Isolates on Fusarium spp. in vitro and Potato Tuber Dry Rot Development in vivo
Mejda Daami-Remadi,Fakher Ayed,Hayfa Jabnoun-Khiareddine,Khaled Hibar
Plant Pathology Journal , 2006,
Abstract: Four Bacillus sp. isolates were individually essayed against Fusarium solani, F. oxysporum f.sp. tuberosi, F. graminearum and F. sambucinum following an in vitro dual culture plate technique and in vivo pre-inoculation tuber treatment. All tested bacterial isolates significantly reduced radial mycelial growth of Fusarium spp., on PDA after 3 days of incubation at 25°C, comparatively to the untreated controls. Light microscopic studies of antagonist x Fusarium spp. in vitro interaction showed several hyphal abnomalies traduced particularly by lesser mycelium density, severe hyphal lysis, lesser sporulation, mycelial cords formation and early chlamydospores induction observed only at the confrontation zone. Potato tubers, cv. Spunta, treated by Bacillus sp. 24 h before individually inoculation by Fusarium species, showed reduced dry rot development after 21 days of incubation at 25-27°C comparatively to untreated controls.
Evaluation of Fungicides for Control of Fusarium Wilt of Potato
Fakher Ayed,Mejda Daami-Remadi,Hayfa Jabnoun-Khiareddine,Khaled Hibar
Plant Pathology Journal , 2006,
Abstract: Four fungicides, which active ingredients are hymexazol, azoxystrobin, fludioxonil and quinoline, are tested in vitro and in vivo against five isolates of Fusarium oxysporum f. sp. tuberosi, causal agent of potato Fusarium wilt. Pathogen radial growth noted on PDA amended with fungicides after incubation for four days at 25°C varied upon pathogen isolates. Mycelial growth of all pathogen isolates was significantly inhibited by fungicide PDA amendment; inhibition obtained by hymexazol treatment reached 77% comparatively to untreated control. All chemical fungicides reduced disease incidence compared to the untreated control. Hymexazol and azoxystrobin are the most active during all the bioassay period. Whereas, fludioxonil and quinoline showed a limited effect in controlling Fusarium wilt development.

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