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Search Results: 1 - 10 of 71 matches for " DWIRINI RETNO GUNARTI "
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Properties of Folate Binding Protein Purified from Cow’s Milk
HAYATI Journal of Biosciences , 2012,
Abstract: Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after -mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.
Isolation and Purification of Thiamine Binding Protein from Mung Bean
HAYATI Journal of Biosciences , 2013,
Abstract: Thiamine has fundamental role in energy metabolism. The organs mostly sensitive to the lack of thiamine levels in the body are the nervous system and the heart. Thiamine deficiency causes symptoms of polyneuritis and cardiovascular diseases. Because of its importance in the metabolism of carbohydrates, we need to measure the levels of thiamine in the body fluids by using an easy and inexpensive way without compromising the sensitivity and selectivity. An option to it is thiamine measurement based on the principle of which is analogous to ELISA, in which a thiamine binding protein (TBP) act by replacing antibodies. The presence of TBP in several seeds have been reported by previous researchers, but the presence of TBP in mung beans has not been studied. This study was aimed to isolate and purify TBP from mung bean. The protein was isolated from mung bean through salting out by ammonium sulphate of 40, 70, and 90% (w/v). TBP has a negative charge as shown by cellulose acetate electrophoresis. The result obtained after salting out by ammonium sulphate was further purified bymeans of DEAE-cellulose chromatography and affinity chromatography. In precipitation of 90% of salting out method, one peak protein was obtained by using affinity chromatography. The protein was analyzed by SDS PAGE electrophoresis. The result of SDS PAGE electrophoresis showed that TBP has a molecular weight of 72.63 kDa.
Retno Ardianti
Jurnal Manajemen dan Kewirausahaan , 2006,
Abstract: Efforts to assess the effect of information technology on firm performance to date, have given mixed results. Some of the research have been unable to conclude the positive effect of information technology on performance. This paper aims to discuss how an organization can better use its information technology assets to realize this positive impact. In order to realize this positive impact, an organization can not just relying itself on investing in information technology equipment. Moreover organization should direct its human resources to innovate on technology that is possessed, fixing its business process, and choosing the right business model by using its technology. Abstract in Bahasa Indonesia : Upaya untuk meneliti dampak teknologi informasi pada kinerja perusahaan selama ini, telah memberikan temuan yang bertolak belakang. Sebagian dari penelitian tersebut tidak dapat menyimpulkan adanya dampak positif dari teknologi informasi terhadap kinerja. Penelitian ini bertujuan untuk membahas bagaimana suatu organisasi dapat menggunakan asset teknologi informasinya dengan lebih baik untuk memperoleh dampak positif dari teknologi tersebut. Untuk dapat merealisasikan dampak positif tersebut, suatu organisasi tidak dapat berhenti pada melakukan investasi pada perangkat teknologi informasi saja, lebih jauh lagi organisasi tersebut harus mengerahkan sumber daya manusianya untuk berinovasi pada teknologi yang dimiliki, memperbaiki proses bisnis yang ada, serta memilih model bisnis yang sesuai dengan memanfaatkan teknologi yang telah dimilikinya. Kata kunci:. teknologi informasi, productivity paradoks, business model.
Retno Gitawati
Media of Health Research and Development , 2012,
Abstract: Drug-drug interactions can initiate undesirable or adverse effects. This could have a clinical implication and could also become serious health problems. Since a large number of drugs are introduced and marketed every year, new interactions between concomitant medications might possibly increase. The safety profile of drugs including the impacts of drug-drug interactions can be provided from pharmacoepidemiology and pharmacovigilance data. There are some mechanisms of drug-dug interactions, i.e. pharmaceutics interaction, pharmacokinetics and pharmacodynamics interactions. However, only pharmacodynamic interaction can be predicted and the effect usually applies equally to any drugs in the same class therapy. On the other hand, pharmacokinetic interactions cannot be predicted or extrapolated to any drugs in the same class therapy because of differences in their pharmacokinetics profiles. Information on how is the mechanism of drug-drug interaction is needed, so the possible adverse effect due to drug-drug interactions can be anticipated. Keywords: drug-drug interaction, adverse effect, pharmacokinetics, pharmacodynamics
Effect of MOS, Baker Yeast, or Noni Leaves Extracts on Antibody Titers to NDV and AI Vaccine of Broilers Fed Corn-mungbean Base Diets
Retno Murwani
International Journal of Poultry Science , 2010,
Abstract: A research was carried out to study the effect of MOS, baker yeast, or Noni leave extracts on broilers antibody titers to NDV and AI vaccine. Broilers were fed corn-mungbean base basal diet. Two hundreds 204 Lohman MB-202 day old chicks were randomly assigned into four treatment diets i.e. 1) commercial diet (Dc), 2) basal diet with addition of MOS (DMOS), (3) basal diet with inactivated baker yeast (DYEAST), (4) basal diet with inactivated baker yeast and Noni leaves extracts (DY+L). Basal diet, commercial diet and water were given ad libitum. NDV and AI vaccine were given simultaneously via subcutaneous on day 4. Serum antibody titers were measured on day 18, 21, 25 and 30 by Haemaglutination Inhibition Test and expressed as Geometric Mean Titer (log2). Antibody titers to NDV and AI vaccine were already detectable two weeks after vaccination (on day 18). There were no significant difference of antibody titers to both vaccines among all groups. These results showed that either MOS, or yeast, or combined yeast and noni leaves extracts can be used in corn-mungbean base diet to support broilers production against NDV and AI routine vaccination.
Effect of Corn or Sorghum in Combination with Soybean Meal or Mungbean as Feed Ingredients on the Serum Antibody Titres to NDV Vaccine in Broiler Chickens
Retno Murwani
International Journal of Poultry Science , 2008,
Abstract: A research was carried out to study the effect of feed types in the diet based on corn or sorghum in combination with soybean meal or broken green beans on titre antibody to NDV vaccine of broilers. Three hundreds day old chicks broilers with initial body weight of 46.35 ± 4.66 grams were randomly assigned into four treatment groups i.e. T1 (diet based on corn and soybean meal), T2 (diet based on corn and green-bean), T3 (diet based on sorghum and soybean meal), T4 (diet based on sorghum and green-bean). Isoenergy and isoprotein diet and water were given ad libitum. NDV vaccines were given via eyedrop on day 4 and intramuscularly on day 21. Serum antibody titres were measured on day 38, 42 and 48 by Haemaglutination Inhibition Test and expressed as Geometric Mean Titre (log2). Antibody titre to NDV vaccine was already detectable on day 38 and the value was highest in T4, reaching 5.2 and lowest in T3 i.e. 3.6 (P < 0.05). On day 42 the titre of all groups were increased reaching a value of greater than 5.0 (protective) except in T3 which remains lower than 5.0 (not protected). On day 48 the highest titre was reached in T2 (6.4), while T3 remained low below 5 (4.2). These results showed that the types of feed ingredients in the diet can influence antibody titre against NDV vaccination.
Exploring Values, Integrity and Anticorruption of Javanese Government Officer
Retno Kumolohadi
International Journal of Social Science and Humanity , 2013, DOI: 10.7763/ijssh.2013.v3.216
Abstract: This study aimed to explore five values orientation, integrity and culture, the core values that can lead javanese government officer to behave corruption or anticorruption. The five values orientation are theoretical, economic, social, aesthetic, politic and religious. Study of Values (S.O.V) from Allport, Vernon and Lindzey based on Spranger theory and A.C.P Integrity Scale based on the Corruption Eradication Commision (C.E.C) are used to collected the data. Subject were 91 head of government office in “X” province in Indonesia, echelon II,II,IV. both gender, under 57 years old, javanese. The results showed that religious was the highest values of most subjects. Subject with high score of integrity had two dominant values, religious and social. Subjects with low integrity had higher values on economic and religious rather than others. Subjects with low integrity could be involved on conflict between two values and perhaps make a wrong decision and to fall into misfortune.
Retno Hastijanti
Dimensi : Journal of Architecture and Built Environment , 2005,
Abstract: Most of Urban violence researches have been done in social-psychology context. One of it is the book by Dr.A.latief Wiyata (2002), Carok, Konflik Kekerasan dan Harga Diri Orang Madura. This book bases on his dissertation on social-cultural; social-economic; and histories context. One of his focuses is on the spreading of Madurese traditional settlement pattern. Based on that, there is an indication that carok ritual has been related to the Madurese traditional settlement. Through the study that is based on the book, is need to be understood that the carok ritual has influenced the Madurese traditional settlement, architecturally. And by discourse analysis it is found that carok ritual has influenced the quality of traditional settlement element intensity, specifically. Then, it is concluded that traditional architecture has a role as violence tradition conservation. Abstract in Bahasa Indonesia : Penelitian tentang kekerasan, banyak dilakukan dalam disiplin ilmu sosial dan psikologi. Salah seorang yang meneliti kekerasan adalah Dr.A.Latief Wiyata, dengan bukunya Carok, Konflik Kekerasan dan Harga Diri Orang Madura (2002). Buku ini ditulis berdasarkan disertasinya. Dijelaskan bahwa carok diteliti sebagai masalah, dalam konteks sosial-kultural, sosial-ekonomis dan historis. Karenanya, penulis memfokuskan salah satunya pada pola permukiman yang tersebar. Berdasarkan hal tersebut, terlihat adanya indikasi pengaruh ritual carok terhadap permukiman tradisional Madura, yang merupakan bentukan arsitektur tradisional Madura. Melalui kajian terhadap buku ini, ingin dipahami bagaimana pengaruh ritual carok dalam permukiman tradisional Madura, secara arsitektural. Dan kemudian melalui analisis wacana, secara khusus, dapat disimpulkan bahwa ritual carok mempengaruhi kwalitas intensitas ikatan elemen-elemen permukiman tradisional Madura. Secara umum, disimpulkan bahwa permukiman tradisional sebagai suatu bentuk arsitektur tradisional berperan untuk melestarikan suatu ritual tradisi kekerasan. Kata kunci: Carok, Elemen Permukiman, Permukiman Tradisional Madura
Retno Hastijanti
Dimensi : Journal of Architecture and Built Environment , 2002,
Abstract: Saminist is Saminism followers. Their culture based on the history of the Samin's rebellion against the Dutch. In 1940, The Dutch exploded Saminist cleansing. For saving their life, the Saminist then made an unwritten-agreement among them to undercover and blend in the middle of society surround, and always assumed the society as sedulur. This unwritten-agreement became their way of life and was reflected in their settlement. This research is qualitative-phenomenology research and using meaning-translation method for analyzing the exclusive space of the Saminist settlement. It had been found that sedulur concept anticipated the formed of exclusive space of Saminist settlement. Abstract in Bahasa Indonesia : Kaum Samin merupakan sekumpulan orang pengikut Saminisme. Budaya Saminisme, berlatar belakang sejarah pemberontakan Samin Surontiko melawan penjajahan Belanda (1890). Pada th.1940, Belanda melakukan 'pembersihan' Kaum Samin, sehingga jumlah mereka menyusut dan tercerai berai. Demi keselamatan mereka, Kaum Samin membuat kesepakatan tak tertulis untuk menyamar dan membaur dengan orang disekitar mereka dan selalu menganggap orang sekeliling mereka adalah sedulur. Kesepakatan tersebut tidak hanya menjadi konsep hidup mereka tetapi tercermin pula dalam permukiman mereka. Melalui penelitian kualitatif-phenomenologis dan penggunaan metoda penerjemahan makna, dilakukan analisa terhadap ruang eksklusif pada permukimannya. Dan terbukti bahwa konsep sedulur mengantisipasi terbentuknya ruang eksklusif pada permukiman kaum Samin. Kata kunci: Kaum samin, Konsep sedulur, Ruang eksklusif.
Variation of Calcium Oxalate (CaOx) Crystals in Porang (Amorphophallus muelleri Blume)  [PDF]
Nurul Chairiyah, Nunung Harijati, Retno Mastuti
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.49217

This research had aimed to determine variations in form of calcium oxalate (CaOx) crystals in porang. Slides for microscopic observation were prepared from sliced leaf, petiole, and tuber of porang. Sliced organs were cleared by using modified clearing method. The parameters that were observed included form and variety of morphology type of CaOx crystals. Microscopic observation showed there were four basic forms of crystals, i.e., druse, raphide, prism and styloid with some variation of diverse types, each of which had 49, 10, 8, and 5 variations. CaOx crystals, based on the size, were grouped into large (20-250 μm) and small (1-15 μm) crystals.

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