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Search Results: 1 - 10 of 56199 matches for " DONG Xiu-zhu "
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A survey of extromophiles project supported by 973
973项目“极端微生物及其功能利用的基础研究”研究进展

ZHANG Min,DONG Xiu-zhu,
张敏
,东秀珠

微生物学报 , 2006,
Abstract: 中国科学院微生物研究所东秀珠研究员主持承担的国家基础研究发展计划———“973”项目“极端微生物及其功能利用的基础研究”,于2004年10月获得批准立项。参加该项目的有:武汉大学、云南大学、浙江大学、吉林大学、国家海洋局三所、中国科学院北京基因组研究所、中国科学院上
Characterization of hydrogen peroxide production by a novel oral streptococci,S. oligofermentans isolated from human oral cavity
一株口腔链球菌新种—寡发酵链球菌产过氧化氢特性的研究

CHEN Wei,TONG Hui-chun,DONG Xiu-zhu,
陈伟
,佟卉春,东秀珠

微生物学报 , 2006,
Abstract: High level of hydrogen peroxide was produced by S.oligofermentans,a novel oral streptococcus isolated from human oral cavity previously in our laboratory.To characterize the hydrogen peroxide production by S.oligofermentans,hydrogen peroxide yields at different growth phases and from different substrates were assayed.The results turned out that hydrogen peroxide production started at the beginning of logarithmic phase,and reached the maximal yield at the early stationary phase.Peptone and yeast extract could be the main substrates for hydrogen peroxide production.Moreover,lactate,as a fermentative product of glucose,can be another substrate for hydrogen peroxide production.Furthermore three oxidases activities possibly associated with H-2O-2 production were assayed,and both activities of lactate oxidase and NADH oxidase were detected under aerobic condition,while pyruvate oxidase activity was not detected in the permeabilized cells of S.oligofermentans,implying that S.oligofermentans could mainly rely on the two enzymes activities for H-2O-2 production.
Phylogenetic Diversity of Prokaryotes and The Main Functional Groups in A UASB Reactor Treating Bean Curd-farm Wastewater
豆腐废水UASB反应器中的原核生物多样性及主要功能菌群

ZHANG Chun-Yang,LIU Xiao-Li,DONG Xiu-Zhu,
张春杨
,刘晓黎,东秀珠

微生物学报 , 2004,
Abstract: 通过构建16S rRNA基因文库,对豆腐废水UASB反应器中颗粒污泥的原核生物多样性进行了分析,并用MPN法对颗粒污泥中的互养产乙酸细菌和产甲烷菌进行了活菌数量测定。结果表明,33%的16S rRNA基因序列属于产甲烷菌,氢和乙酸盐营养型的产甲烷菌在颗粒污泥中数量最多,分别为1.1×10.9个/mL和4.5×10.8个/mL。低GC革兰氏阳性菌和δ-变形菌纲分支的细菌也是颗粒污泥中的主要菌群,它们的16S rRNA序列分别占22%和9%,其中互养产乙酸细菌在颗粒污泥中的数量可达4.5×10.7个/ml。绿色非硫细菌是另一类丰度很高的细菌,其16S rRNA序列占文库的12%。对各类微生物在颗粒污泥中可能的作用进行了讨论。通过研究不仅了解了特定环境中的微生物组成,还为从中分离特异类群的微生物提供了指导。
Diversity of prokaryotic microorganisms
原核微生物的多样性

DONG Xiu-Zhu,HONG Jun-Hua Institute of Microbiology,Chinese Academy of Sciences,Beijing,
东秀珠
,洪俊华

生物多样性 , 2001,
Abstract: Microorganisms are an important group of organism mainly performing catabolic metabolism, which display extreme abundance in biodiversity. However, due to their microscopic sizes, especially prokaryotes with simple unicellular structure, their asexual propagation, their rapid replication and the lack of base line inventory of their groups and quantity, the biodiversity of microorganisms has so far not been studied thoroughly and has been paid little attention compared with macro-organisms. In the present paper, the biodiversity of microorganisms is reviewed briefly, with consideration of the following aspects: species diversity, diversity of phylogenetic lines, physiological diversity and genetic diversity. The aim is to improve awareness of investigation of microorganism resources, not only in the scientific community but also in society as a whole.
A new hydrogen-producing strain and its characterization of hydrogen production
一个新的高温产氢菌及产氢特性的研究

NIU Li-li,LIU Xiao-li,CHEN Shuang-ya,DONG Xiu-zhu,
牛莉莉
,刘晓黎,陈双雅,东秀珠

微生物学报 , 2006,
Abstract: An anaerobic, thermophilic, hydrogen-producing strain T42 was obtained from a hot spring of South Mountain District, Tibet. Cells are Gram-positive, mobile rod-shaped. Spores were not observed. Temperature range for growth is 32 degrees C to 69 degrees C (optimum temperature, 60 degrees C - 62 degrees C), and pH range for growth is 5.0 to 8.8 (optimum pH, 7.0 - 7.5). The generation time is around 30 min. Organic nitrogen sourc is required for growth. Strain T42 utilizes a wide range of carbohydrates, including starch, dextrin, sucrose, cellobiose, fructose, maltose, ribose, glycogen and galactose. Acetate, ethanol, H2 and CO2 are the end products of glucose fermentation. The (G + C) content of strain T42 is 31.2 mol%. Phylogenetic analysis based on the 16S rDNA sequence similarity indicates that strain T42 is the closest relative to Thermobrachium celere and Caloramator indicus. Biological characteristics and phylogenetic analysis of the 16S rDNA gene indicate the new strain belongs to the genus Thermobrachium. Strain T42 produces H2 from glucose at maximal level when growing at 62 degrees C and initial pH 7.2, the hydrogen yields and maximal hydrogen production rate are 1.06 mol H2/mol glucose and 24.0 mmol H2/gDW/h, respectively. Strain T42 also produced H2 by fermentating from a variety of carbohydrates. 20 mmol/L Magnesium and 2 mmol/L iron increase the hydrogen production content by 20% and 23.3%, respectively, but nickel has no effect on the hydrogen production. In the co-culture of strain T42 and methane-producing strain M. thermautotrophicus Z245, hydrogen pressure is dramatically decreased, meanwhile deduced H2 production and the consumption of glucose are increased markedly by 2.8 fold and 1 fold, and the ratio of acetate/ethanol is enhanced froml to 1.7.
Research on EPA network without collecting switchers
支持线性拓扑的EPA网络研究

JIANG Xiu-zhu,FENG Dong-qin,XU Zhao,CHU Jian,
姜秀柱
,冯冬芹,徐钊,褚健

计算机应用 , 2009,
Abstract: Because EPA protocol realizes accessing media without collision, it supports nodes to share accessing media. Under the specifications of EPA, three EPA network structures were designed without collecting switchers supporting line topology. Methods followed to realize the network form and its real-time property was analyzed.
The Study on Somatic Cell Cloned Cattle in China
我国体细胞克隆牛的研究进展

CHENG Jin-hua,ZHU Hua-bin,DAI Yun-ping,SUN Xiu-zhu,WANG Dong,HAO Hai-sheng,
程金华
,朱化彬,戴蕴平,孙秀柱,王栋,郝海生

中国生物工程杂志 , 2006,
Abstract: Because of the important role of cloning technique in husbandry production, therapy of the disease, study of the basic theory and the conservation of animal resource, the efficiency of the cloning technique was becoming the present focus of the study. The factors related to the cloning technique were reviewed. The effect of different cell lines on the efficiency of somatic cell cloned cattle were different, the blastocyst rate, pregnant rate and calving rate of fetal cells and the cumulus cells were higher than ones of adult fibroblast. The study about refrigerant conservation of cloned embryos and oocyte indicated that the vitrification refrigeration method could be used to freeze the oocyte and the cloned cattle embryos. The new progress of the study on mammalian cell clone and the efficiency of somatic cell clone were also discussed, and the urgent problem in somatic cell clone was presented.
Food-grade Gene Expression Systems for Lactic Acid Bacteria
乳酸菌食品级基因表达系统

ZHANG Zhen-Zhong,CHEN Xiu-Zhu JIA Shi-Fang CHEN Mei-Ling HUAN Lian-Dong,
张振中
,陈秀珠,贾士芳,陈美玲,还连栋

生物工程学报 , 2002,
Abstract: Lactic acid bacteria (LAB) are important industrial microor ganism used in the production and preservation of foodstuffs. Recently, considerable advances have been made in the genetics and molecular biology of LAB. These have resulted in the construction of food-grade gene expression systems for these bacteria. This paper aims to review the essential features for food-grade systems, food-grade selection markers, food-grade controlled gene expression and food-grade inducible signaling molecule, and recent developments on food-grade cloning and expression systems for LAB.These gene expression systems have great potential for studies on gene expression and regulation in LAB and a variety of bioprocessing application in industrial fermentations.
Methanogenic archaea and their mediated methanogenic pathways in cold wetland
低温湿地甲烷古菌及其介导的甲烷产生途径

JIANG N,CHEN Zi-juan,CAO Yi,TIAN Jian-qing,WANG Yan-fen,DONG Xiu-zhu,
蒋娜
,陈紫娟,曹轶,田建卿,王艳芬,东秀珠

微生物学通报 , 2013,
Abstract: Cold wetlands contribute great portion of the global methane. This is attributed to the inhabited diverse microbes including methanogenic archaea, where they convert the organic materials to methane. This review summarized the recent studies on the diversity of methanogenic archaea, and methane emission pathways in the cold wetlands and their correlation with environmental parameters. The so-far studies indicated that acetate is the main methanogenic precursor in the wetlands located in cold regions, hydrogenothophic methanogenesis occurs mainly in wetlands located in the warm regions and acidic peat, while methanol- and methyl amine-derived methanogensis restricts in ocean and saline water. Zoige wetland situates on Qinghai-Tibet Plateau locating in the low latitude region but at high altitude. Our previous study indicated the significant methanogenic contribution of methanol in this wetland. Accordingly, the methylotrophic and aceticlastic Methanosarcinales and hydrogenotrphic Methanomicrobiales constitute the predominant wetland archaeal community. However, methanogenic pathways and methanogen community structures vary with the wetland soil types and their pH as well as the vegetations, like methanol-derived methane accounting for 17% total methane flux in Eleocharis valleculosa growing soil, while acetate contribute the main methane in Carex muliensis planted soil in Zoige wetland. Though playing an important role in methane emission of cold wetlands, the so-far cultured cold-adaptive methanogenic strains are very few. The low-temperature-responsive genomics analyses show that a global gene reperpoire may involve in cold adaptation in methanogenic archaea.
Influences of H on the Adsorption of a Single Ag Atom on Si(111)-7 × 7 Surface
Lin Xiu-Zhu,Li Jing,Wu Qi-Hui
Nanoscale Research Letters , 2009,
Abstract: The adsorption of a single Ag atom on both clear Si(111)-7 × 7 and 19 hydrogen terminated Si(111)-7 × 7 (hereafter referred as 19H-Si(111)-7 × 7) surfaces has been investigated using first-principles calculations. The results indicated that the pre-adsorbed H on Si surface altered the surface electronic properties of Si and influenced the adsorption properties of Ag atom on the H terminated Si surface (e.g., adsorption site and bonding properties). Difference charge density data indicated that covalent bond is formed between adsorbed Ag and H atoms on 19H-Si(111)-7 × 7 surface, which increases the adsorption energy of Ag atom on Si surface.
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