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Search Results: 1 - 5 of 5 matches for " DIDY SOPANDIE "
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Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L.
RADITE TISTAMA,UTUT WIDYASTUTI,DIDY SOPANDIE,AKIHO YOKOTA
HAYATI Journal of Biosciences , 2012,
Abstract: We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.
ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P
Ratna Yuniati,Utut Widyastuti1,2,,Didy Sopandie,Akiho Yokota
Makara Seri Sains , 2011,
Abstract: Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutivelyand is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequentlyused as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotidesequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of thisresearch was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin genesequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed fromconserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNAsequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% withactin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins fromPrunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet,they can be used as reference in J. curcas L. gene expression analysis.
Isolation and Effect of Al-Tolerant Phosphate Solubilizing Microorganism for Production and Phosphate Absorption of Grasses and Phosphour Dissolution Mechanism
PDMH Karti,S Yahya,D Sopandie,S Hardjosuwignyo
Journal of Animal Production , 2012,
Abstract: The objective of this research was to study the isolation and effect of Al-tolerant phosphate solubilizing microorganisms to growth, production of grasses and phosphate dissolution mechanism. The planting materials used were S. splendida and C. gayana pols. The treatment consisted of four selected isolates, namely Po = without phosphate solubilizing bacteria(PSB), P1 = RJM.30.2, P2 = FT.3.2, P3 = FT.3.4, P4 = B8016495, P5 = B8016498, P6 = the mixture from four isolates P2-P5. Observed variables were pH, shoot and root dry weight, and P absorption. The best phosphate solubilizing microorganism on acid soil were FP.3.2, FP.3.3, B8016495 and B8016498. Phosphate solubilizing microorganism could not yet increase shoot and root dry weight production on grasses S. splendida and C. gayana, but had shown increasing P shoot and root content and P uptake. On grass S. splendida the best isolate to increase P shoot and root of shoot and root, organic acid. The best phosphate solubilizing microorganism on acid soil content and P uptake was FT.3.3. On grass C gayana the best isolate to increase P shoot and root content and P uptake were RJM.30.2. and FT.3.3. Organic acid exudated by FT.3.3. were oxalic and acetic acid as phosphate dissolution mechanism. Keywords: phosphate solubilizing microorganism, acid soil, forage grasses, Setaria splendida, Chloris gayana
Efficacy of tibolone and raloxifene for the maintenance of skeletal muscle strength, bone mineral density, balance, body composition, cognitive function, mood/depression, anxiety and quality of life/well-being in late postmenopausal women ≥ 70 years: Study design of a randomized, double-blind, double-dummy, placebo-controlled, single-center trial
Didy E Jacobsen, Monique M Samson, Yvonne Schouw, Diederick E Grobbee, Harald JJ Verhaar
Trials , 2008, DOI: 10.1186/1745-6215-9-32
Abstract: We recruited 318 elderly women in our single-center randomized, double-blind, double-dummy, placebo-controlled trial. Participants were randomized to tibolone 1.25 mg (Org OD 14, Organon NV, the Netherlands) plus placebo, raloxifene 60 mg (Evista?, Eli Lilly, United States) plus placebo or two placebo tablets daily for 24 months.The primary aim is to determine if there is a difference between tibolone and placebo or if there is a difference between raloxifene and placebo. Primary endpoints are muscle strength and bone mineral density. The secondary endpoints are postural balance, body composition, cognitive function, anxiety, mood and quality of life. The secondary aim is to determine if there is a difference between tibolone and raloxifene.The measure of effect is the change from the baseline visit to the visits after 3 months, 6 months, 12 months, and 24 months. A follow-up measurement is planned at 30 months to determine whether any effects are sustained after cessation of the study. By December 2007 the blind will be broken and the data analyzed.NTR: 1232Aging is associated with muscle atrophy, physical frailty, and impaired cognitive function. This functional decline can severely affect quality of life and reduce the likelihood of a person being able to live independently. Women are more vulnerable to the effects of this age-related muscle loss because their peak muscle mass is lower than that of men and around the time of menopause they experience an additional 15% loss of muscle mass [1-3]. Hormonal replacement therapy (HRT) in postmenopausal women may prevent muscle strength decline [2,4-9]. Unfortunately, however conventional hormone replacement therapy has serious side-effects such as an increased risk of breast cancer, thrombo-embolism, cholecystitis, stroke and coronary events, especially in elderly women. Unopposed estrogen replacement therapy increases the risk of endometrial carcinoma. [10,11]For this reason, newer drugs, such as tibolone, a synthetic
Physiological Adaptation and Biomass Production of Macroptilium bracteatum Inoculated with AMF in Drought Condition
S. Sowmen,L. Abdullah,P. D. M. H. Karti,D. Sopandie
Media Peternakan , 2012,
Abstract: The objective of this experiment was to evaluate the effect of drought stress and mycorrhizal inoculation on physiological adaptation and biomass production of Macroptilium bracteatum. This experiment was arranged in completely randomized design with four treatments: M0 (no AM + watered), M1 (AM + watered), M2 (no AM + drought), and M3 (AM + drought) with three replicates. The observed variables were soil water content, leaf water potential, leaf relative water content, leaf proline, leaf water soluble carbohydrate (WSC), root and shoot dry weight. Data were analyzed by ANOVA and differences between treatments were tested by DMRT. Drought treatments (M2 and M3) significantly (P<0.05) decrease soil water content, leaf water potential, leaf relative water content and increased the leaf proline content. The result in root and shoot dry weight appear that M1 treatment was significantly different (P<0.05) with treatment M0, M2, and M3. For leaf WSC, M0 and M2 treatments were significantly different (P<0.05) with treatment M1 and M3. It is concluded that mycorrhiza inoculation was more effective on M. bracteatum, in drought stress. One mechanism of drought resistance of M. bracteatum is the accumulation of osmotic compounds proline. Therefore, proline can be used as an indicator of drought resistance in leguminous plants.
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