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Search Results: 1 - 10 of 47 matches for " DIAH RATNADEWI "
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LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS
DIAH RATNADEWI
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2006,
Abstract: The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the po ssible roles of the protein in plant tolerance to environmental stress. This gene was fused to gfp marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions.
GFDD4-1 Gene Expression in Physcomitrella patens and Homologous Gene in Arabidopsis thaliana in Response to Abiotic Stress
DIAH RATNADEWI,WOLFGANG FRANK
HAYATI Journal of Biosciences , 2005,
Abstract: A number of abiotic stress responsive genes have been identified from various plant species through reverse genetic strategy. A group of genes are involved in plant responses to stress; they are activated by diverse stress conditions and through different mechanisms. One single gene can be induced by several different stress factors; on the other hand, a number of genes can be up-regulated by a single factor. In Physcomitrella patens, through Northern hybridization, the transcript level of the gene GFDD4-I was detected to be markedly increased by ABA, dehydration and cold, but not by salinity and osmotic stress. In Arabidopsis thaliana, a homologous gene to GFDD4-1 namely At2g47770, was confirmed to fulfill similar function as in P. patens: it is inducible by various abiotic stress treatments, i.e. ABA, dehydration, salinity, and cold. Inducible genes in response to abiotic stress factors may be responsible for plant tolerance to those factors.
Quinoline Alkaloids in Suspension Cultures of Cinchona ledgeriana Treated with Various Substances
DIAH RATNADEWI,SUMARYONO
HAYATI Journal of Biosciences , 2010,
Abstract: Cinchona alkaloids are in extensive uses, not only for drugs but also for soft drink industries. They are harvested from the bark of trees Cinchona spp. after certain ages and therefore are available over a limited time. Cell culture is an alternative way to continuously produce such secondary metabolites in a much shorter time. Various substances were added in the normal growth media to promote quinoline alkaloids production by cell cultures of Cinchona ledgeriana. At the sixth week of culture, quinine and cinchonine contents were suppressed by paclobutrazol (PBZ), abscisic acid (ABA), or even by precursor tryptophan, while cinchonidine content was enhanced by 0.2 mg/l tryptophan to 43 fold of that produced by untreated cells (2.8% dry weight). At the seventh week of culture, the production of quinine and quinidine started to grow whereas the production of cinchonine and cinchonidine tended to decrease. An addition of 5 mg/l PBZ to culture media yielded the highest level of total quinine/quinidine after seven weeks, e.g. quinine 11 times more abundant and quinidine 23 fold higher compared to the untreated cells. Particularly the level of quinine which is the most demanded for medical and industrial purposes still need to be improved to approach to or even higher than that of extracted from the conventional source.
Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets
SUMARYONO,WIRDHATUL MUSLIHATIN,DIAH RATNADEWI
HAYATI Journal of Biosciences , 2012,
Abstract: Sago palm (Metroxylon sagu Rottb.), grown mostly in the tropics, is one of the most productive carbohydrateproducing crops. However, it is still underutilized. Tissue culture of sago through somatic embryogenesis has been developed. The plantlets derived from somatic embryos, however, are usually weak with few leaves and roots and have low survival rates during acclimatization. Carbohydrate is commonly added into culture medium as an energy source and an osmotic agent. Research was conducted to determine a suitable carbohydrate for plantlets growth in order to produce vigorous plantlets of sago. The basal medium used was a modified MS medium with a half-strength of salts. Different types of carbohydrate (sucrose, maltose, glucose, and fructose) at various concentrations (30, 45, and 60 g/l) were added into the medium. A single 2 cm plantlet derived from somatic embryo was cultured on a culture tube. Each treatment consisted of 15 plantlets. The cultures were incubated in a culture room with light intensity at 20 μmol/m2/s and temperature at 26 oC. The results show that different types and concentrations of carbohydrate influenced the growth of sago plantlets significantly, but there was no interaction between the two factors. Sucrose was better than other types of carbohydrate, and the concentration of 30 g/l was better than concentrations of 45 or 60 g/l for the growth and vigor of sago plantlets. Medium with a sucrose level at 30 g/l gave the best performance of sago plantlets based on plantlet height, leaf number, biomass fresh weight, stem diameter, and rooting percentage.
Evaluation of GSMaP Daily Rainfall Satellite Data for Flood Monitoring: Case Study—Kyushu Japan  [PDF]
Martiwi Diah Setiawati, Fusanori Miura
Journal of Geoscience and Environment Protection (GEP) , 2016, DOI: 10.4236/gep.2016.412008
Abstract: In this paper, the Global Satellite Mapping of Precipitation Moving Vector with Kalman filter (GSMaP_MVK) was evaluated and corrected at daily time scales with a spatial resolution of 0.1°; latitude/longitude. The reference data came from thirty-four rain gauges on Kyushu Island, Japan. This study focused on the GSMaP_MVK’s ability to detect heavy rainfall patterns that may lead to flooding. Statistical analysis was used to evaluate the GSMaP_MVK data both quantitatively and qualitatively. The statistical analysis included the relative bias (B), the mean error (E), the Nash-Sutcliffe coefficient (CNS), the Root Mean Square Error (RMSE) and the correlation coefficient (r). In addition, Generalized Additive Models (GAMs) were used to conduct GSMaP_MVK data correction. The results of these analyses indicate that GSMaP_MVK data have lower values than observed data and may be significantly underestimated during heavy rainfall. By applying GAM to bias correction, GSMaP_MVK’s ability to detect heavy rainfall was improved. In addition, GAM for bias correction could effectively be applied for significant underestimates of GSMaP_ MVK (i.e., bias of more than 55%). GAM is a new approach to predict rainfall amount for flood and landslide monitoring of satellite base precipitation, especially in areas where rain gauge data are limited.
ANALISIS TREN PENELITIAN PANGAN FUNGSIONAL: KATEGORI BAHAN SERAT PANGAN [Functional Food Research Trend Analysis: Dietary Fiber Category]
Diah Anggraeni Jatraningrum
Jurnal Teknologi dan Industri Pangan , 2012,
Abstract: Functional food, such as probiotic, prebiotic, dietary fiber, vitamin, flavonoid etc, is one of the convergent products between foods and pharmaceutical industry. Dietary fiber, as a part of functional food, is showing a rapid increment in publications and patents. This review will try to give more information about the current advancement in dietary fiber research in Indonesia versus global research trend and also to compare patentability of research in dietary fiber. There are five technology in dietary fiber that will be highlighted; formulation-process-fortification, extraction-isolation-purification, fermentation, metabolic and biotechnology. Research evaluation between dietary fiber research data in the Indonesian Institute of Science (LIPI) and global publications and patents show that dietary fiber have chance to be developed become new product with a convergent category in functional food. Dietary fiber with probiotic or prebiotic for is as an example.
KOHESIVITAS SUAMI ISTRI DI USIA MADYA
Diah Anindita,Khoiruddin Bashori
HUMANITAS , 2012,
Abstract: In the middle age, adjustment to developmental tasks will affect marital relationship. This study aimed to determine the cohesiveness of husband and wife who have entered middle adulthood, covering aspects of solidarity, marital satisfaction, interest, and affiliates and also the factors that affect the cohesiveness of husband and wife when the marriage age of 20 years or more. Subjects are three pairs of husband and wife who are legally married for over 20 years and each individual’s age between 40-60 years old. This qualitative research used the case study approach and collected data by semi-structured interviews and nonparticipant observation. The results showed that the cohesiveness of husband and wife owned in middle age is different in each pair. In the first couple showed low cohesion, high on the second couple, and medium for the third couple. In the third couple, there is a change before the peak of the conflict in mid-2011 by now. Factors that affect the cohesiveness of husband and wife in middle age is a social perception, social support, emotional regulation, a period of quiet in the middle ages, the intensity of togetherness, understanding of gender, temperament, and love.
Do the Indonesians Receive the Dental Care Treatment They Need? A Secondary Analysis on Self-Perceived Dental Care Need
Diah Ayu Maharani
ISRN Dentistry , 2012, DOI: 10.5402/2012/769809
Abstract:
Do the Indonesians Receive the Dental Care Treatment They Need? A Secondary Analysis on Self-Perceived Dental Care Need
Diah Ayu Maharani
ISRN Dentistry , 2012, DOI: 10.5402/2012/769809
Abstract: Increasing access for citizens to health services, including dental care, is one of the primary targets of the Indonesian Ministry of Health. To assess progress toward this goal, we sought to describe the magnitude of unmet needs for dental care among Indonesians. Secondary data of nationally representative surveys conducted from 2003 to 2007 were analysed to describe the associations between unmet needs for dental care in different demographic groups. In average, 2.28% of the Indonesian population reported perceiving need for dental care and 0.74% reported utilizing dental care. The average of unmet need was 72.04%. Logistic regression analysis indicated that respondents who lived in rural areas, who were uninsured, had higher odds ratios in reporting unmet dental care needs. Perceived need for and utilization of dental care among Indonesians was found to be low. Moreover, the unmet need for dental care is relatively high. 1. Introduction In a WHO [1] publication, it is stated that one of the primary targets of the Indonesian Ministry of Health is to increase access for citizens to health services, including dental care. But facts indicated that dental health problems can still be found in almost every area in Indonesia. The Indonesian Basic Health Survey 2007 showed that the rates of edentulous were 2% of the whole population, and only 4.5% of them used dentures. The National DMF-T (Decayed Missing Filling Tooth) index was 4,85. The biggest component was missing teeth (M-T), which was 3.86, describing that in average every Indonesian has 4 teeth extracted or was indicated for extraction. Moreover, 17.6% of Indonesians aged 65 or older had lost all their teeth. This percentage is far from the WHO target of less than 5% edentulous for year 2010 [2]. One of the oral health objectives of the Indonesian Ministry of Health for 2010 is to increase in the proportion of Indonesians who utilize dental health care annually [3]. Given this situation, it is important to evaluate the extent of those who have a need for dental care but do not receive any dental treatments or in other words those who had unmet dental care needs. This kind of evaluation is important to study the impact of government’s policy to dental health in Indonesia. Nevertheless, there is a lack of information about how dental health care have changed over time causing the inability to assess the effects of government policies, whether these policies were leading toward or away from greater social justice. Therefore, the objective of this study was to assess the magnitude of unmet need for
Fermentation product of RS3 inhibited proliferation and induced apoptosis in colon cancer cell HCT-116  [PDF]
Endang Yuli Purwani, Diah Iskandriati, Maggy Thenawidjaja Suhartono
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.38145
Abstract: Cell free supernatant containing short chain fatty acid (SCFA) resulted from fermentation of resistant starch type three (RS3) by Clostridium butyricum BCC B2571 or Eubacterium rectale DSM 17629 were investigated for their ability to inhibit proliferation and induce apoptosis of human colon cancer cell line HCT-116. HCT-116 was cultured in complete medium and after 50% confluent, incubation was continued for another 48 hours in the absence or presence cell free supernatant containing SCFA mixture at butyrate levels up to 10 mM. The study revealed that the proliferation inhibition effect was higher (>80%) on HCT-116 treated with supernatant of C. butyricum BCC B2571 than that (<70%) of HCT-116 treated with supernatant of E. rectale DSM 17629. The cells were induced to undergo apoptosis by both supernatant. The apoptosis occured through mitochondrial pathway by changing the expression of gene Bcl-2 and Bax, thus incresed the Bax/Bcl-2 ratio by more than 3.5 fold. The protein caspase-3 was increased by more than 250% in the presence of the cell free supernatant.
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