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Search Results: 1 - 10 of 380484 matches for " Débora de Mello Gon?ales Sant'Ana "
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Miedo y Deshumanización: una Visión Crítica sobre la Realidad Cotidiana en el Laboratorio de Anatomía Humana
Castilho,Marco Antonio SantAna; Oda,Juliano Yasuo; SantAna,Débora de Mello Gonales;
International Journal of Morphology , 2009, DOI: 10.4067/S0717-95022009000300043
Abstract: human anatomy has the human body as an important source of studies. it uses non-claimed, usually pauper, bodies delivered as a benefit to society without a counterpart, thus deserving full respect. however, within current society, death is not faced as something proper to life, what results in a state of fear, agony and loath within the human anatomy laboratory. therefore, negation mechanisms which may generate dehumanization are produced. to be confronted with someone else's death is the same as to be confronted with our own death. within this context, professors and technicians have great responsibility on having the students aware that the cadaver is the expression of life and respect, as such concepts will be very important for the students' future professional life. with this article we aim at reflecting on the reality of death and respect inside the human anatomy laboratory.
Miedo y Deshumanización: una Visión Crítica sobre la Realidad Cotidiana en el Laboratorio de Anatomía Humana Fear and Deshumanization: A Critical View on the Daily Routine of the Human Anatomy Laboratory
Marco Antonio SantAna Castilho,Juliano Yasuo Oda,Débora de Mello Gonales SantAna
International Journal of Morphology , 2009,
Abstract: La anatomía humana tiene como importante fuente de estudio al cuerpo humano. Ella utiliza cuerpos no reclamados, usualmente de indigentes, cuerpos entregados como un beneficio a la sociedad sin nada pedir nada a cambio, siendo dignos de pleno respeto. Sin embargo, preocupa que la muerte no es mirada socialmente como algo natural a la vida, llevando la convivencia en el laboratorio de anatomía humana a un estado de miedo, angustia y repulsión. Con eso se produce un mecanismo de negación, que puede generar deshumanización hacia la pieza anatómica. Ser confrontado con la muerte de otro, es lo mismo que confrontarse con nuestra propia muerte. En este contexto, los profesores y técnicos tienen una gran responsabilidad en discutir y reflejar la cuestión, de modo que puedan promover la idea que el cadáver es la expresión de vida y respeto, pues tal concepto será de gran importancia para la futura vida profesional de los estudiantes. Con este artículo, nuestro objetivo es reflexionar sobre la realidad de la muerte y el respeto en el laboratorio de anatomía humana. Human anatomy has the human body as an important source of studies. It uses non-claimed, usually pauper, bodies delivered as a benefit to society without a counterpart, thus deserving full respect. However, within current society, death is not faced as something proper to life, what results in a state of fear, agony and loath within the human anatomy laboratory. Therefore, negation mechanisms which may generate dehumanization are produced. To be confronted with someone else's death is the same as to be confronted with our own death. Within this context, Professors and technicians have great responsibility on having the students aware that the cadaver is the expression of life and respect, as such concepts will be very important for the students' future professional life. With this article we aim at reflecting on the reality of death and respect inside the human anatomy laboratory.
ANATOMICAL ASPECTS OF THE PROXIMAL FEMUR OF ADULT BRAZILIANS
Silva,Valter José da; Oda,Juliano Yasuo; Mello Gonales Sant'Ana,Débora de;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000400008
Abstract: this study had the purpose of enlarging the data on the brazilian people, providing anatomic fundamentals that allow a better comprehension of clinical and pathological states involving the hip joint. sixty-six human femurs, 33 right and 33 left, were used, coming from the laboratory of human anatomy of the universidade paranaense - unipar. all the bones had adult characteristics, but no sex, race or age identifications. none of them presented visible abnormalities or signs of arthrosis. the following morphometric measures were made: angle of the femur neck, circumference of the femur head and neck, length of the femur neck and of the whole bone. the comparative analysis of the angle of the femur neck between the right and left sides of adults demonstrated values similar to those described in the classic literature for adults with mean significantly smaller on the right side (122.5o) than on the left side (125.6o). as for the length of the femur neck in our study we observed larger values on the left side (23.5 mm) than on the right side (22.3 mm). these data demonstrate the asymmetry of the bones of brazilians, with greater angle and neck on the left side than on the right side, agreeing with authors that state that the left inferior limb is dominant relative to the left. for the other measures of the femur, circumference of the head and neck, differences were not observed between the right and left sides. we suggest that other studies should be carried out to enlarge the scarce literature concerning the normal measures of brazilians
ANATOMICAL ASPECTS OF THE PROXIMAL FEMUR OF ADULT BRAZILIANS ASPECTOS ANATóMICOS DE LA EPíFISIS PROXIMAL DEL FéMUR EN BRASILE OS ADULTOS
Valter José da Silva,Juliano Yasuo Oda,Débora de Mello Gonales Sant'Ana
International Journal of Morphology , 2003,
Abstract: This study had the purpose of enlarging the data on the Brazilian people, providing anatomic fundamentals that allow a better comprehension of clinical and pathological states involving the hip joint. Sixty-six human femurs, 33 right and 33 left, were used, coming from the Laboratory of Human Anatomy of the Universidade Paranaense - UNIPAR. All the bones had adult characteristics, but no sex, race or age identifications. None of them presented visible abnormalities or signs of arthrosis. The following morphometric measures were made: angle of the femur neck, circumference of the femur head and neck, length of the femur neck and of the whole bone. The comparative analysis of the angle of the femur neck between the right and left sides of adults demonstrated values similar to those described in the classic literature for adults with mean significantly smaller on the right side (122.5o) than on the left side (125.6o). As for the length of the femur neck in our study we observed larger values on the left side (23.5 mm) than on the right side (22.3 mm). These data demonstrate the asymmetry of the bones of Brazilians, with greater angle and neck on the left side than on the right side, agreeing with authors that state that the left inferior limb is dominant relative to the left. For the other measures of the femur, circumference of the head and neck, differences were not observed between the right and left sides. We suggest that other studies should be carried out to enlarge the scarce literature concerning the normal measures of Brazilians El propósito de este estudio fue ampliar los datos óseos sobre individuos brasile os, otorgando un aporte anatómico que permita facilitar una mejor comprensión de cuadros clínicos y patológicos que involucran la articulación de la cadera. Fueron utilizados 66 fémures, (33 de cada lado) proporcionados por el Laboratorio de Anatomía Humana de la Universidad Paranaense (UNIPAR). Todos los huesos presentaban características adultas, sin identificación de sexo, raza ni edad. Ningún hueso presentaba alteraciones aparentes o signos de artrosis. Se realizaron las siguientes mediciones morfométricas: ángulo del cuello del fémur, circunferencia de la cabeza del fémur y del cuello, longitud del cuello del fémur y de todo el hueso. El análisis comparativo del ángulo del cuello del fémur, entre los lados derecho e izquierdo de adultos, presentó valores iguales a los descritos en la literatura clásica para adultos, con promedio significativamente menor para el lado derecho (122,5°) comparado con el lado izquierdo (125,6°). Respecto a la
Intestinal Ascending Colon Morphometrics in Rats Submitted to Severe Protein Malnutrition
Hermes,Catchia; Azevedo,Jorge Fernandes de; Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gonales;
International Journal of Morphology , 2008, DOI: 10.4067/S0717-95022008000100001
Abstract: this paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). in the following 90 days, the rats of the control group received a 24% protein chow as the malnourished group received 4% protein chow. the animals were submitted to euthanasia according to the anesthetic protocol. colon segments were collected and submitted to routine histological processing. the cuts were stained with he and histochemical techniques for mucines. the morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of goblet cells, the depth of the crypt and the height of the enterocytes as well as their nucleus on malnutritioned animals. the data suggest that protein malnutrition causes alterations on adult rat ascending colon intestinal morphometrics, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells, and crypts.
Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
Franco,Cássia Luiza de Marins; Sant'Ana,Débora de Mello Gonales; Araújo,Eduardo José de Almeida;
International Journal of Morphology , 2010, DOI: 10.4067/S0717-95022010000200026
Abstract: the effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. six 90-day-old wistar rats (rattus norvegicus) were assigned into two groups: cg (control group, n = 3) and mg (malnourished group, n = 3). cg animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. after 90 days, part of the jejunum was collected and subjected to routine histology. he-staining and histochemical techniques - periodic acid schiff (pas) and alcian blue (ab), ph 2.5/ph 1.0 - were used for the detection of glycoconjugates in 4μm -thick transverse cuts. morphometric analysis of the he-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to pas and ab ph 2.5, whereas the number of cells reactive to ab ph 1.0 increased. protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium.
Morfometría de la Pared Intestinal del Colon Ascendente de Ratas Sometidas a Desnutrición Proteica Severa Intestinal Ascending Colon Morphometrics in Rats Submitted to Severe Protein Malnutrition
Catchia Hermes,Jorge Fernandes de Azevedo,Eduardo José de Almeida Araújo,Débora de Mello Gonales Sant'Ana
International Journal of Morphology , 2008,
Abstract: This paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). In the following 90 days, the rats of the control group received a 24% protein chow as the malnourished group received 4% protein chow. The animals were submitted to euthanasia according to the anesthetic protocol. Colon segments were collected and submitted to routine histological processing. The cuts were stained with HE and histochemical techniques for mucines. The morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of goblet cells, the depth of the crypt and the height of the enterocytes as well as their nucleus on malnutritioned animals. The data suggest that protein malnutrition causes alterations on adult rat ascending colon intestinal morphometrics, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells, and crypts. El objetivo de este trabajo fue verificar los efectos de la desnutrición proteica severa sobre la morfometría de la pared intestinal del colon ascendente de ratas adultas. Fueron utilizadas 12 ratas (90 días de edad), divididas en dos grupos: control (n=5) y desnutrido (n=7). En los 90 días siguientes, las ratas del grupo control recibieron ración con 24% de contenido proteico y los del grupo desnutrido con 4%. Los animales fueron eutanasiados de acuerdo al protocolo anestésico. Segmentos del colon fueron retirados y sometidos a procesamiento histológico de rutina. Los cortes fueron te idos con HE y técnicas histoquímicas para mucinas. El análisis morfométrico mostró la mantención de la pared total y del grosor de la túnica muscular, y reducción en el espesor de la túnica mucosa, en el número de células caliciformes, en la profundidad de las criptas y en la altura de los enterocitos y de sus núcleos, en los animales desnutridos. Los datos obtenidos sugieren que la desnutrición proteica provoca alteraciones en la morfometría intestinal del colon ascendente de ratas adultas, principalmente en tejidos de alto índice de renovación celular como la mucosa y, consecuentemente, de sus estructuras como los enterocitos, células caliciformes y criptas.
Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition Atrofia de la Pared Intestinal y Aumento de la Secreción de Sulfomucinas en el Epitélio Yeyunal de Ratas Sometidas a Intensa Desnutrición Protéica
Cássia Luiza de Marins Franco,Débora de Mello Gonales Sant'Ana,Eduardo José de Almeida Araújo
International Journal of Morphology , 2010,
Abstract: The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4μm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium. Fueron evaluados los efectos de la desnutrición proteica severa sobre la morfometría de la pared intestinal del yeyuno de ratas adultas . Para esto, se utilizaron seis ratas (Rattus norvegicus) Wistar, con 90 días de edad, distribuidas en dos grupos: GC (grupo control, n=3) y el GD (grupo desnutrido, n=3). Los animales del GC recibieron ración con un contenido proteico de 26% y los del GD ración con 4% de proteínas. Después de 90 días de experimentación, parte del yeyuno fue retirado y sometido a un procesamiento histológico de rutina. Cortes transversales de 4 μm fueron te idos con HE y técnicas histoquímicas (Periodic Acid Schifff (PAS) y Alcian Blue (AB) pH 2,5 y pH 1,0.) para evidenciar glicoconjugados. El análisis morfométrico te idos con HE demostró reducción en el grosor total de la pared del yeyuno, especialmente de las túnicas muscular y mucosa, además se observó una disminución en la altura de los entericitos. Los cortes te idos con técnicas histoquímicas revelaron que el número de células caliciformes reactivas al PAS y al AB pH 2,5 se mantuvo; por otro lado, hubo un aumento en el número de células reactivas al AB pH 1,0. Se concluye que la desnutrición proteica severa provoca atrofia de la pared yeyunal y aumento de la secreción de sulfomucinas en el epitelio intestinal.
Morphologic and quantitative study of the myenteric neurons of the jejunum of malnourished rats (Rattus norvegicus)
MIRANDA NETO MARCíLIO HUBNER DE,MOLINARI SONIA LUCY,STABILLE SANDRA REGINA,SANT'ANA DéBORA DE MELLO GONALES
Arquivos de Neuro-Psiquiatria , 1999,
Abstract: We studied the effects of maternal proteic desnutrition on the neurons of the myenteric plexus of the jejunum of rats from Rattus norvegicus species. It was used litters of female rats which received diet with normal proteic level during gestation and lactation (group NN), normal diet during gestation and hypoproteic diet during lactation (group ND); hypoproteic diet during gestation and normal diet during lactation (group DN); hypoproteic diet during both gestation and lactation (group DD). After weaning all the animals received diet of normal proteic level until the 60th day of age, when they were killed. The jejunum of the animals was subjected to whole-mount preparations stained by the method of Giemsa and used for the morphologic and quantitative analyses of the neurons of the myenteric plexus. We verified that maternal proteic malnutrition does not cause decrease on the number of myenteric neurons per unit area of jejunum in rats, but elicits mechanisms which assure that, when the animal again receives normal proteic level diet (22%) there occurs storage of proteic material on the cytoplasm of the neurons, thus rendering them larger and strongly basophylic.
NADH-DIAPHORASE POSITIVE MYENTERIC NEURONS OF THE AGLANDULAR REGION OF THE STOMACH OF RATS (Rattus norvegicus) SUBJECTED TO DESNUTRITION NURONAS MIOENTéRICAS NADH-DIAFORASA POSITIVAS DE LA REGIóN NO GLANDULAR DEL ESTóMAGO DE RATONES (Rattus norvegicus) SOMETIDOS A DESNUTRICIóN
Sonia Lucy Molinari,Carlos Alexandre Fernandes,Larissa Renata de Oliveira,Débora de Mello Gonales Sant'Ana
Revista chilena de anatomía , 2002,
Abstract: The stomach carried out movements characterized by potent peristaltic slow waves which serve mixture, mechanical digestion and slow emptying through the pylorus. The control of this movement is made by hormone action and neural activity, especially the intrinsic innervation by the neurons of the myenteric plexus. Once the condition of desnutrition can cause morphological alterations even upon the neurons this work was proposed with the purpose of verifying the effects of protein desnutrition lasting 120 days on the quantitative aspects of the NADH-diaphorase positive neurons. It was used 10 adult rats aging 90 days, which were divided in two groups, control and disnurtured. The rats of the disnurtured group (n=5) were subjected to a diet of 8% protein level, and those of the control group (n=5) received chow with normal protein level (22%). After 120 days, the aglandular stomach was subjetec to the histoenzymologic technique of the NADH-diaphorase for neuronal evidenciation. The aglandular regions was divided in a region near the major gastric curvature and a region anterior to the limitant ridge. According to the sampling method of counting, the observed neurons in 40 microscopic fields (6.64 mm2) of each region in all the animals of the two groups were counted using light microscope with 40X objective. We verified that the neurons can be isolated or grouped into ganglia. Next to the limitant ridge it was found a mean of 570.8 neurons in the control group and 718.4 in the disnurtured group. On the other hand, next to the major gastric curvature, we observed 25.8 neurons in the control group and 52.6 neurons in the disnurtured group. The low-protein diet resulted in less body growth with a weight decrease of about 30.33% when compared to the control animals, as well as a decrease in the area of the stomach of 20.13%. We concluded that the neurons are not uniformly distributed in the stomach wall and that the disnurtured animals showed less neuronal dispersion, thus exhibiting a greater neuronal density per mm2 El estómago realiza movimientos caracterizados por potentes ondas peristálticas lentas, útiles en la mezcla del bolo alimenticio, la digestión mecánica y su lenta liberación por el píloro. El control de este movimiento se realiza por acción hormonal y por actividad nerviosa, destacando la inervación intríseca que representan las neuronas del plexo mioentérico. Considerando que un estado de desnutrición puede provocar alteraciones morfológicas, incluso neuronales, se ha propuesto este trabajo, con el objetivo de verificar los efectos provocados por
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