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Search Results: 1 - 10 of 218 matches for " Cucunubá Zulma "
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Haga usted el diagnóstico. Segunda parte
Nohora Marcela Mendoza,María Eugenia Leal,Zulma Milena Cucunubá
Biomédica , 2011,
Abstract:
Haga usted el diagnóstico. Primera parte
Nohora Marcela Mendoza,María Eugenia Leal,Zulma Milena Cucunubá
Biomédica , 2011,
Abstract:
Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients
Flechas Ivonne,Cuellar Adriana,Cucunubá Zulma,Rosas Fernando
BMC Infectious Diseases , 2009,
Abstract: Background Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude T. cruzi extract and three recombinant antigens: the T. cruzi kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the T. cruzi HSP-70 protein192-433, and the entire Trypanosoma rangeli HSP-70 protein. Methods Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests. Results The T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass. Conclusion T. cruzi KMP-11 and the T. rangeli HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease. Polarising the IgG1 subclass of the IgG response to T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.
Validez de los estudios de asociación entre geohelmintos e incidencia de malaria: ?Debería impactar las políticas de salud?
Fernández, Julián Alfredo;Idrovo, álvaro Javier;Cucunubá, Zulma Milena;Reyes, Patricia;
Revista Brasileira de Epidemiologia , 2008, DOI: 10.1590/S1415-790X2008000300004
Abstract: introduction: the global distribution of malaria and soil-transmitted helminths is widely overlapped. some studies suggest a possible association between helminth infection and incidence of malaria. objectives: to identify the available epidemiologic evidence and to assess the validity of these studies. methods: a systematic review was carried out in specialized databases. the studies identified were critically analyzed and ranked according to the u.s. preventive services task force's classification. the major methodological limitations of each study were identified. results: six studies on the topic were found. only two studies had a high evidence level (level i), three had level ii-2, and one had level iii-3. there are important methodological limitations for clarifying the association between soil-transmitted helminths and the incidence of malaria. conclusion: it is too early to discuss the potential public health implications of these findings, given the lack of studies and limited validity of the evidence available. further studies with new methodological considerations could improve the knowledge on the association. however, it is more important to carry out actions on structural determinants to control and prevent the occurrence of both diseases.
Characterization of the National Malaria Diagnostic Network, Colombia, 2006-2010 Caracterización de la Red Nacional de Diagnóstico de Malaria, Colombia, 2006-2010
Olga Lucía Ospina,Liliana Jazmín Cortés,Zulma Milena Cucunubá,Nohora Marcela Mendoza
Biomédica , 2011,
Abstract: Introduction. The implementation and development of activities of the quality assurance system of malaria diagnosis, allows the adequate operation of the national diagnostic network, needed to strengthen prevention and control actions of this important public health problem. Objective. To characterize the malaria diagnosis network in Colombia between 2006 and 2010. Materials and methods. A retrospective study was made by reviewing the annual reports of malaria diagnosis network activities that were sent by the Public Health Laboratories (PHL) between 2006 and 2010. The study included analysis of diagnostic coverage in population at risk and an evaluation of activities and training to people responsible for malaria diagnosis. Results. Malaria diagnostic coverage has increased in Colombia, from a 53% of municipalities covered in 2006 to 80% in 2010. The number of places that perform diagnosis increased in 31% with a significant increase, for the same period, of the number of microscopists (50%) and laboratories (30%) for a total of 1195 and 1780 respectively, registered in 2010. During the period of study, the percentage of PHL that carried out at least 3 of the activities of the quality assurance system for the diagnostic network at local level was 67%. Conclusions. It is necessary to continue with strengthening the malaria diagnosis network to provide timely and adequate diagnosis in order to reduce the morbidity and mortality by malaria. Introducción. La implementación y desarrollo de actividades del sistema de gestión de la calidad del diagnóstico de malaria, permiten el adecuado funcionamiento de la red nacional de diagnóstico, necesario para fortalecer acciones de prevención y control de este evento importante en salud pública Objetivo. Caracterizar la Red de Diagnóstico de Malaria en Colombia entre 2006 y 2010. Materiales y métodos. Se realizó un estudio retrospectivo mediante la revisión de los informes anuales de actividades de la Red de Diagnóstico de Malaria, enviados por los Laboratorios de Salud Pública (LSP) entre 2006 y 2010. Se analizó cobertura de diagnóstico en población a riesgo, las actividades de evaluación del desempe o y las capacitaciones al personal que realiza el diagnóstico. Resultados. La cobertura del diagnóstico de malaria se ha incrementado en el país, pasando del 53% de los municipios en 2006 al 80% en 2010. El número de sitios que realizan diagnóstico aumentó en un 31% con un incremento en el número de microscopistas (50%) y de laboratorios (30%) para un total de 1.195 y 1.780 respectivamente, registrados en 2010. En el pe
Acute Chagas disease in Colombia: a rarely suspected disease. Report of 10 cases presented during the 2002-2005 period Enfermedad de Chagas aguda en Colombia, una entidad poco sospechada. Informe de 10 casos presentados en el periodo 2002 a 2005
Rubén Santiago Nicholls,Zulma Milena Cucunubá,Angélica Knudson,Astrid Carolina Flórez
Biomédica , 2010,
Abstract: Introduction. In Colombia, reported cases of acute Chagas disease are sporadic. Objective. Ten cases were described that had been reported to the Parasitology Laboratory of the Colombian National Health Institute between December 2002 and November 2005. Materials and methods. Information from clinical records, epidemiological report forms, laboratory and blood tests was collated. In addition the following data were compiled: demographic variables, clinical findings, results of laboratory tests and other exams (such as peripheral blood smears), IFAT for IgG antibodies, isolation in culture medium, inoculation in mice, polymerase chain reaction tests and isoenzyme eletrophoresis. Results. All the cases presented in known endemic areas for Chagas disease transmission in Colombia. Three cases were from Putumayo Province, two each from the provinces of Arauca, Casanare, Norte de Santander and one from Santander Province. The probable mode of transmission was vector-borne. Seven cases presented in adults aged 18 to 50, three in children aged 6 months to 2 years. Seven were male and three were female. The most frequent symptom was fever in nine cases. Signs of portal of entry were rare; only one patient presented a possible Roma a′s sign. Three patients presented myocarditis, two acute cardiac failure and one cardiac tamponade. Parasitemia was evident in nine cases; five had positive IgG serological tests; five cases were confirmed through parasite isolation; isoenzyme electrophoresis showed Trypanosoma cruzi group I. Conclusions. Clinical variability prevailed. In none of the cases was a clinical diagnosis suspected. The diagnosis was made and confirmed through laboratory tests alone. The results highlight the importance of including this disease in the differential diagnosis of febrile syndrome in endemic regions due to its good response to etiological treatment and thereby preventing its progression to the chronic phase. Introducción. Los casos de enfermedad de Chagas aguda informados en Colombia son esporádicos. Objetivo. Describir 10 casos notificados al Grupo de Parasitología del Instituto Nacional de Salud entre 2002 y 2005. Materiales y métodos. Utilizando información de historias clínicas, fichas epidemiológicas, informes de resultados de exámenes de laboratorio y análisis de sangre, se tabularon variables demográficas, hallazgos clínicos, paraclínicos y de laboratorio. Se realizaron extendidos de sangre periférica, identificación serológica para IgG mediante inmunofluorescencia indirecta, aislamiento en cultivo e inoculación en ratones, pruebas de rea
Caracterización de la Red Nacional de Diagnóstico de Malaria, Colombia, 2006-2010
Ospina,Olga Lucía; Cortés,Liliana Jazmín; Cucunubá,Zulma Milena; Mendoza,Nohora Marcela; Chaparro,Pablo;
Biomédica , 2012,
Abstract: introduction. the implementation and development of activities of the quality assurance system of malaria diagnosis, allows the adequate operation of the national diagnostic network, needed to strengthen prevention and control actions of this important public health problem. objective. to characterize the malaria diagnosis network in colombia between 2006 and 2010. materials and methods. a retrospective study was made by reviewing the annual reports of malaria diagnosis network activities that were sent by the public health laboratories (phl) between 2006 and 2010. the study included analysis of diagnostic coverage in population at risk and an evaluation of activities and training to the people responsible for malaria diagnosis. results. malaria diagnostic coverage has increased in colombia, from 53% of municipalities covered in 2006 to 80% in 2010. the number of places that perform diagnosis increased by 31% with a significant increase, for the same period, of the number of microscopists (56%) and laboratories (30%), to 1,195 and 1,780 respectively, registered in 2010. during the period of study, the percentage of phl that carried out at least 3 of the activities of the quality assurance system for the diagnostic network at local level was 67%. conclusions. it is necessary to continue strengthening the malaria diagnosis network to provide timely and adequate diagnosis in order to reduce the morbidity and mortality by malaria.
Molecular Epidemiology of Human Oral Chagas Disease Outbreaks in Colombia
Juan David Ramírez,Marleny Montilla,Zulma M. Cucunubá,Astrid Carolina Floréz,Pilar Zambrano,Felipe Guhl
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002041
Abstract: Background Trypanosoma cruzi, the causative agent of Chagas disease, displays significant genetic variability revealed by six Discrete Typing Units (TcI-TcVI). In this pathology, oral transmission represents an emerging epidemiological scenario where different outbreaks associated to food/beverages consumption have been reported in Argentina, Bolivia, Brazil, Ecuador and Venezuela. In Colombia, six human oral outbreaks have been reported corroborating the importance of this transmission route. Molecular epidemiology of oral outbreaks is barely known observing the incrimination of TcI, TcII, TcIV and TcV genotypes. Methodology and Principal Findings High-throughput molecular characterization was conducted performing MLMT (Multilocus Microsatellite Typing) and mtMLST (mitochondrial Multilocus Sequence Typing) strategies on 50 clones from ten isolates. Results allowed observing the occurrence of TcI, TcIV and mixed infection of distinct TcI genotypes. Thus, a majority of specific mitochondrial haplotypes and allelic multilocus genotypes associated to the sylvatic cycle of transmission were detected in the dataset with the foreseen presence of mitochondrial haplotypes and allelic multilocus genotypes associated to the domestic cycle of transmission. Conclusions These findings suggest the incrimination of sylvatic genotypes in the oral outbreaks occurred in Colombia. We observed patterns of super-infection and/or co-infection with a tailored association with the severe forms of myocarditis in the acute phase of the disease. The transmission dynamics of this infection route based on molecular epidemiology evidence was unraveled and the clinical and biological implications are discussed.
Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients
Ivonne D Flechas, Adriana Cuellar, Zulma M Cucunubá, Fernando Rosas, Víctor Velasco, Mario Steindel, María Thomas, Manuel López, John González, Concepción Puerta
BMC Infectious Diseases , 2009, DOI: 10.1186/1471-2334-9-186
Abstract: Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.The T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass.T. cruzi KMP-11 and the T. rangeli HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease.Polarising the IgG1 subclass of the IgG response to T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.Antibodies against several parasitic antigens are copious during blood-borne parasite infections such as malaria and Chagas' disease. These humoral immune responses have been used for diagnosis, following up individuals throughout the course of a natural infection, vaccination protocols and evaluating drug therapy efficacy. However, little is known about these antibodies' specific role or profile according to disease phases. Concerning humoral responses, it has been described that antibodies against repeat and/or evolutionary conserved sequences are highly predominant in parasitic infections such as that caused by Trypanosoma cruzi, the aetiological agent of Chagas' disease [1-3]. B lymphocytes and antigen specific antibodies seem to be crucial for controlling acute infection during the course of T. cruzi infection and cou
Asymptomatic Plasmodium spp. infection in Tierralta, Colombia
Cucunubá, Zulma Milena;Guerra, ángela Patricia;Rahirant, Sonia Judith;Rivera, Jorge Alonso;Cortés, Liliana Jazmín;Nicholls, Rubén Santiago;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000700007
Abstract: with the aim of determining the prevalence of asymptomatic plasmodium spp. infection by thick smear and pcr and its association with demographic and epidemiological characteristics in the village of nuevo tay, tierralta, córdoba, colombia, a cross-sectional population study was carried out, using random probabilistic sampling. venous blood samples were taken from 212 people on day 0 for thick smear and pcr. clinical follow-up and thick smears were carried out on days 14 and 28. the prevalence of plasmodium spp. infection was 17.9% (38/212; 95% ci: 12.5-23.3%) and the prevalence of asymptomatic plasmodiumspp. infection was 14.6% (31/212; 95% ci: 9.6-19.6%). plasmodium vivax was found more frequently (20/31; 64.5%) than plasmodium falciparum (9/31; 29%) and mixed infections (2/31; 6.5%). a significantly higher prevalence of asymptomatic infection was found in men (19.30%) than in women (9.18%) (prevalence ratio: 2.10; 95% ci: 1.01-4.34%; p = 0.02). people who developed symptoms had a significantly higher parasitemia on day 0 than those who remained asymptomatic, of 1,881.5 ± 3,759 versus 79 ± 106.9 (p = 0.008). pcr detected 50% more infections than the thick smears. the presence of asymptomatic plasmodium spp. infection highlights the importance of carrying out active searches amongst asymptomatic populations residing in endemic areas.
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