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Search Results: 1 - 10 of 433896 matches for " Cristina Martínez-Fernández de la Cámara "
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Altered Antioxidant-Oxidant Status in the Aqueous Humor and Peripheral Blood of Patients with Retinitis Pigmentosa
Cristina Martínez-Fernández de la Cámara, David Salom, Ma Dolores Sequedo, David Hervás, Cristina Marín-Lambíes, Elena Aller, Teresa Jaijo, Manuel Díaz-LLopis, José María Millán, Regina Rodrigo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074223
Abstract: Retinitis Pigmentosa is a common form of hereditary retinal degeneration constituting the largest Mendelian genetic cause of blindness in the developed world. It has been widely suggested that oxidative stress possibly contributes to its pathogenesis. We measured the levels of total antioxidant capacity, free nitrotyrosine, thiobarbituric acid reactive substances (TBARS) formation, extracellular superoxide dismutase (SOD3) activity, protein, metabolites of the nitric oxide/cyclic GMP pathway, heme oxygenase-I and inducible nitric oxide synthase expression in aqueous humor or/and peripheral blood from fifty-six patients with retinitis pigmentosa and sixty subjects without systemic or ocular oxidative stress-related disease. Multivariate analysis of covariance revealed that retinitis pigmentosa alters ocular antioxidant defence machinery and the redox status in blood. Patients with retinitis pigmentosa present low total antioxidant capacity including reduced SOD3 activity and protein concentration in aqueous humor. Patients also show reduced SOD3 activity, increased TBARS formation and upregulation of the nitric oxide/cyclic GMP pathway in peripheral blood. Together these findings confirmed the hypothesis that patients with retinitis pigmentosa present reduced ocular antioxidant status. Moreover, these patients show changes in some oxidative-nitrosative markers in the peripheral blood. Further studies are needed to clarify the relationship between these peripheral markers and retinitis pigmentosa.
The role of phenotypic plasticity on the proteome differences between two sympatric marine snail ecotypes adapted to distinct micro-habitats
Mónica Martínez-Fernández, María de la Cadena, Emilio Rolán-Alvarez
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-65
Abstract: We compared qualitative and quantitative differences in protein expression between pools of both ecotypes from different environments (field and laboratory conditions). The results suggested that ecotype differences may affect about 7% of the proteome in agreement with previous studies, and moreover these differences are basically insensitive to environmental changes. Thus, observed differences between wild ecotypes can be mainly attributed to genetic factors rather than phenotypic plasticity.These results confirm the mechanism of adaptation already proposed in this species and a minor role of phenotypic plasticity in this ecological speciation process. In addition, this study provides a number of interesting protein spots potentially involved in adaptation, and therefore candidates for a future identification.The ability of populations to adapt to a particular habitat is a key topic in evolutionary biology because the exploitation of new niches is a key component of the speciation process [1]. In order to survive in heterogeneous habitats, organisms can adopt three main evolutionary strategies [2]. One is to fix a generalist genotype more or less suitable over a broad range of environmental conditions. A second possibility is to adapt to a particular environmental condition by heritable variation in a particular trait (direct genetic determination). Finally, a third option is to (genetically) control the sensitivity of the genotype to changes in the environment (i.e. indirect genetic determination; phenotypic plasticity). The first and third strategies are expected to be typical of organisms with relatively high dispersal abilities, while the second strategy is preferentially expected for species with restricted dispersal ability, although different exceptions are known [3]. In addition, in the case of sedentary organisms the level of environmental fluctuation might also affect which strategy is used [4,5].Phenotypic plasticity is defined as occurring when the phen
Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors
Olalla Otero-Estévez,Mónica Martínez-Fernández,Lorena Vázquez-Iglesias,María Páez de la Cadena,Francisco J. Rodríguez-Berrocal,Vicenta S. Martínez-Zorzano
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140816986
Abstract: In previous studies we described a decreased alpha-L-fucosidase activity in colorectal tumors, appearing as a prognostic factor of tumoral recurrence. The aim of this work was to extend the knowledge about tissue alpha-L-fucosidase in colorectal cancer by quantifying the expression of its encoding gene FUCA1 in tumors and healthy mucosa. FUCA1 mRNA levels were measured by RT-qPCR in paired tumor and normal mucosa tissues from 31 patients. For the accuracy of the RT-qPCR results, five candidate reference genes were validated in those samples. In addition, activity and expression of alpha-L-fucosidase in selected matched tumor and healthy mucosa samples were analyzed. According to geNorm and NormFinder algorithms, RPLP0 and HPRT1 were the best reference genes in colorectal tissues. These genes were used for normalization of FUCA1 expression levels. A significant decrease of more than 60% in normalized FUCA1 expression was detected in tumors compared to normal mucosa ( p = 0.002). Moreover, a gradual decrease in FUCA1 expression was observed with progression of disease from earlier to advanced stages. These findings were confirmed by Western blot analysis of alpha-L-fucosidase expression. Our results demonstrated diminished FUCA1 mRNA levels in tumors, suggesting that expression of tissue alpha-L-fucosidase could be regulated at transcriptional level in colorectal cancer.
La dependencia funcional del anciano institucionalizado valorada mediante el índice de Barthel
Fuente Sanz,M.a Milagros de la; Bayona Marzo,Isabel; Fernández de Santiago,Francisco José; Martínez León,Mercedes; Navas Cámara,Francisco José;
Gerokomos , 2012, DOI: 10.4321/S1134-928X2012000100004
Abstract: the aim of this article is to evaluate the dependency level of 156 elderly people (101 women and 55 men), residing in private nursing homes in soria (spain), using the barthel index, in order to establish an interdisciplinary performance planning for the prevention and reversal of dependency situations. conclusions: the dependency level of institutionalized elderly people increases by several factors such as, age, sex, the time they have been institutionalized and a bad perception of their health status.
Eficacia de la gabapentina y de la amitriptilina en el dolor neuropático del diabético
Vidal,M. A.; Martínez-Fernández,E.; Martínez-Vázquez de Castro,J.; Torres,L. M.;
Revista de la Sociedad Espa?ola del Dolor , 2004,
Abstract: neuropathic pain is a type of chronic pain that has a great impact on the patients. diabetes mellitus is one of the main causes of neuropathic pain. it is one of the most complex pain syndromes, with diverse symptoms and signs that change over time both in number and severity. it is usually associated to clinical manifestations that interfere with social and job activity, with the resulting impairment of quality of life. its treatment is difficult and, in general, scarcely satisfactory due to the lack of knowledge regard-ing its physiopathological mechanisms. antidepressants have been considered until now as the first-line drugs, but due to their limited effectiveness and their side effects, therapeutic alternatives have been looked for. through a search in the medline database, we reviewed controlled trials that have studied the effectiveness of amytriptiline and gabapentine (gbp) for the management of diabetic neuropathic pain. for years, antidepressants have been considered the best drugs for this type of pain, amytriptiline being the most frequently used. currently, anti-epileptics are being increasingly used for the management of this type of pain, and gbp seems to be the most effective one. anticholinergic side effects of the amytriptiline restrict its use, particularly in aged patients. gbp seems to be a well-tolerated drug, with dizziness and somnolence as its most frequent side effects, so it seems to be a good alternative as first-line therapy for neuropathic pain in aged patients. only two controlled and randomized studies have been published that compare both treatments. however, these studies cannot be considered conclusive due to their small sample size. gbp can be considered the first-line treatment for diabetic neuropathic pain. the medication must be decided individually for each patient, based on the risk-benefit ratio.
Neuropatía diabética: Eficacia de la amitriptilina y de la gabapentina
Vidal,M. A.; Martínez-Fernández,E.; Martínez-Vázquez de Castro,J.; Torres,L. M.;
Revista de la Sociedad Espa?ola del Dolor , 2004,
Abstract: neuropathic pain is a form of chronic pain that has a great impact on the patients. diabetes mellitus is one of the major causes of neuropathic pain. it is one of the most complex painful syndromes, with different symptoms and signs that vary over time both in their number and severity. the accompanying clinical signs affect the social and occupational activity of the patient, with the resulting decrease in the quality of life. its treatment is difficult and, in general, unsatisfactory due to the lack of knowledge regarding its pathophysiological mechanisms. antidepressants have been, up to now, the first-line drugs, but given its limited effectiveness and side effects, other therapeutic alternatives have been looked for. through a search in the medline database, we reviewed controlled trials that study the effectiveness of amitriptyline and gabapentin for the management of painful diabetic neuropathy. antidepressants have been considered for years the first-line treatment for neuropathic pain in the diabetic patient, amitriptyline being the most widely used. antiepileptics are increasingly important the management of this type of pain and they are believed to be the most effective ones. the anticolinergic side effects of amitriptyline restrict its use, the most common side effects being dizziness and somnolence, so it seems to be a good alternative as first-line treatment for neuropathic pain in aged patients. just two controlled and randomized studies have been published that compare both treatments. however, these studies cannot be considered conclusive due to their small sample size. gabapentin can also be considered a first-line treatment for neuropathic pain in the diabetic. the election of one or the other drug must be made in each individual case based on the risk-benefit relation.
Neuropatía diabética: Eficacia de la amitriptilina y de la gabapentina Diabetic neuropathy: Effectiveness of amitriptyline and gabapentin
M. A. Vidal,E. Martínez-Fernández,J. Martínez-Vázquez de Castro,L. M. Torres
Revista de la Sociedad Espa?ola del Dolor , 2004,
Abstract: El dolor neuropático es una forma de dolor crónico con gran repercusión sobre los pacientes. La diabetes mellitus es una de las prinicipales causas de de dolor neuropático. Es uno de los síndromes dolorosos más complejos, con diversos síntomas y signos que fluctúan con el tiempo tanto en su número como en intensidad. Suele asociar clínica acompa ante repercutiendo sobre la actividad social y laboral, con la consiguiente disminución de la calidad de vida. Su tratamiento es difícil y, en general, poco satisfactorio, debido a la falta de conocimiento de los mecanismos fisiopatológicos. Los antidepresivos han sido, hasta ahora, los fármacos de primera elección, pero dada su limitada eficacia y efectos secundarios, se han tenido que buscar alternativas terapéuticas. Mediante una búsqueda en la base de datos del Medline, hemos analizado los estudios controlados que estudian la eficacia de la amitriptilina y de la gabapentina en el tratamiento del dolor neuropático del diabético. Los antidepresivos se han considerado, durante a os, tratamiento de primera elección en el dolor neuropático del diabético, siendo la amitriptilina el más empleado. Es creciente la importancia de los anticomiciales para el tratamiento de este tipo de dolor, y se apunta a la gabapentina como el más efectivo. Los efectos secundarios anticolinérgicos de la amitriptilina limitan su uso, sobre todo en los pacientes de mayor edad. La gabapentina parece ser un fármaco bien tolerado, siendo los efectos secundarios más frecuentes el mareo y la somonolencia, por lo parece una buena alternativa como tratamiento de primera elección del dolor neuropático en pacientes a osos. únicamente se han publicado dos estudios controlados y aleatorizados que comparan ambos tratamientos. No obstante, estos estudios no pueden considerarse concluyentes debido al peque o tama o muestral. La gabapentina puede considerarse de primera elección en el tratamiento del dolor neuropático del diabético. La elección de uno u otro fármaco debe hacerse de forma individualizada, basándose en la relación riesgo/beneficio. Neuropathic pain is a form of chronic pain that has a great impact on the patients. Diabetes mellitus is one of the major causes of neuropathic pain. It is one of the most complex painful syndromes, with different symptoms and signs that vary over time both in their number and severity. The accompanying clinical signs affect the social and occupational activity of the patient, with the resulting decrease in the quality of life. Its treatment is difficult and, in general, unsatisfactory due to the lack of knowl
Eficacia de la gabapentina y de la amitriptilina en el dolor neuropático del diabético Effectiveness of gabapentine and amytriptiline in the diabetic neuropathic pain
M. A. Vidal,E. Martínez-Fernández,J. Martínez-Vázquez de Castro,L. M. Torres
Revista de la Sociedad Espa?ola del Dolor , 2004,
Abstract: El dolor neuropático es una forma de dolor crónico con gran repercusión sobre los pacientes. La diabetes mellitus es una de las prinicipales causas de dolor neuropático. Es uno de los síndromes dolorosos más complejos, con diversos síntomas y signos que fluctúan con el tiempo tanto en su número como en intensidad. Suele asociar clínica acompa ante repercutiendo sobre la actividad social y laboral, con la consiguiente disminución de la calidad de vida. Su tratamiento es difícil y, en general, poco satisfactorio, debido a la falta de conocimiento de los mecanismos fisiopatológicos. Los antidepresivos han sido, hasta ahora, los fármacos de primera elección, pero dada su limitada eficacia y efectos secundarios, se han tenido que buscar alternativas terapéuticas. Mediante una búsqueda en la base de datos del Medline, hemos analizado los estudios controlados que estudian la eficacia de la amitriptilina y de la gbapentina (GBP) en el tratamiento del dolor neuropático del diabético. Los antidepresivos se han considerado, durante a os, tratamiento de primera elección en el dolor neuropático del diabético, siendo la amitriptilina el más empleado. Es creciente la importancia de los anticomiciales para el tratamiento de este tipo de dolor, y se apunta a la GBP como el más efectivo. Los efectos secundarios anticolinérgicos de la amitriptilina limitan su uso, sobre todo en los pacientes de mayor edad. La GBP parece ser un fármaco bien tolerado, siendo los efectos secundarios más frecuentes el mareo y la somnolencia, por lo que parece una buena alternativa como tratamiento de primera elección del dolor neuropático en pacientes a osos. únicamente se han publicado dos estudios controlados y aleotorizados que comparan ambos tratamientos. No obstante, estos estudios no pueden considerarse concluyentes debido al peque o tama o muestral. La GBP puede considerarse de primera elección en el tratamiento del dolor neuropático del diabético. La elección de uno u otro fármaco debe hacerse de forma individualizada, basándose en la relación riesgo/beneficio. Neuropathic pain is a type of chronic pain that has a great impact on the patients. Diabetes mellitus is one of the main causes of neuropathic pain. It is one of the most complex pain syndromes, with diverse symptoms and signs that change over time both in number and severity. It is usually associated to clinical manifestations that interfere with social and job activity, with the resulting impairment of quality of life. Its treatment is difficult and, in general, scarcely satisfactory due to the lack of knowledge regard-ing its
Deformación plástica en compresión de Si3N4 policristalino: consideraciones sobre los modelos de solución-reprecipitación y de formación de cavidades
Martínez-Fernández, J.,Valera-Feria, F.,Ramírez de Arellano-López, A.
Revista de Metalurgia , 2001,
Abstract: Compressive creep of silicon nitride has been studied in several commercial and experimental grades of creep-resistant Si3N4. The temperature range was 1400-1500°C, and inert atmosphere was used to avoid long-term degradation of the samples. The creep rates at a given temperature showed more than one order of magnitude of grade to grade variability. Microstructural analysis showed the presence of cavities after deformation. When analyzed by a classic power-law equation the creep parameters n= 1 for all the grades, while Q varied from 444 to 951 kJ/mol. The activity of diffusional mechanisms is discussed. Alternatively, the results have been analyzed by means of the Luecke-Wiederhorn cavitation model. La fluencia del nitruro de silicio se ha estudiado en varios materiales comerciales y experimentales. El rango de temperaturas ha sido 1.400-1.500 °C y, para evitar la degradación de las muestras, se usó atmósfera inerte. Los resultados de la fluencia para una temperatura dada mostraron una variación de más de un orden de la magnitud entre las distintas muestras. El análisis microestructural mostró la presencia de cavidades después de la deformación. El exponente de tensión, usando la ecuación clásica de fluencia, fue 1 para todos los tipos, mientras que la energía de activación varió entre 444 y 951 kJ/mol. Se discute la actividad de los mecanismos difusionales. Alternativamente, los resultados se han analizado por medio del modelo de cavitación de Luecke-Wiederhorn.
Hyperhomocysteinemia and methylenetetrahydrofolate reductase 677C→T and 1298A→C mutations in patients with inflammatory bowel disease
Fernández-Miranda,C.; Martínez Prieto,M.; Casis Herce,B.; Sánchez Gómez,F.; Gómez González,P.; Martínez López,J.; Sáenz-López Pérez,S.; Gómez de la Cámara,A.;
Revista Espa?ola de Enfermedades Digestivas , 2005, DOI: 10.4321/S1130-01082005000700005
Abstract: background: hyperhomocysteinemia has been recently described in patients with inflammatory bowel disease (ibd), that could be related to the increased risk for thrombosis that exists in this disease. the aim of this study was the assessment of hyperhomocysteinemia in patients with ibd and its relation among vitamin b12 and folate levels, and methylenetetrahydrofolate reductase (mthfr) 677c→ t and 1298a→c mutations. patients and methods: fifty two consecutive patients with ibd were studied (29 women and 23 men); age: mean (standard deviation 41.7 [11.9] years) and 186 controls with no difference in age and gender. hyperhomocysteinemia was considered as homocysteine levels higher than mean plus two standard deviations of the control group (≥ 13 μmol/l). results: patients had an elevated prevalence of hyperhomocysteinemia (17.3 vs. 3.7%; p = 0.002) and lower folate (7.6 [4.1] vs. 8.9 [3.7] ng/ml; p = 0.01) and b12 vitamin levels (499 [287] vs. 603 [231] pg/ml; p = 0.003). homocysteinemia was higher (14.3 [5.8] vs. 9.1 [3.9] μmol/l; p = 0.006) in 6 patients (11.5%) that had suffered thromboembolism. frequency of mthfr 677c→t (13.5 vs. 11.3%; p = 0.66) and 1298a→c (7.8 vs. 7.0%; p = 0.76) mutations was not increased in patients. odds ratio (or) for ibd in hyperhomocysteinemic patient was 5.51, 95% confidence interval (ci), 1.81-16.76; p = 0.002). hyperhomocysteinemia was negatively associated with feminine gender (or 0.08, 95% ci 0.01-0.49; p = 0.006) and folate levels (or 0.04, 95%ci: 0.007-0.20; p < 0.001). conclusions: hyperhomocysteinemia is associated with ibd and low folate levels, and could be involved in development of thromboembolism. mthfr 677c→t and 1298a→c mutations are not related with the disease.
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