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Search Results: 1 - 10 of 463041 matches for " Cory A. Leonard "
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Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi
Carrie E. Jolly,Cory A. Leonard,J. Russell Hayman
International Journal of Microbiology , 2010, DOI: 10.1155/2010/523654
Abstract: Microsporidia spore surface proteins are an important, under investigated aspect of spore/host cell attachment and infection. For comparison analysis of surface proteins, we required an antibody control specific for an intracellular protein. An endoplasmic reticulum-associated heat shock protein 70 family member (Hsp70; ECU02_0100; “C1”) was chosen for further analysis. DNA encoding the C1 hsp70 was amplified, cloned and used to heterologously express the C1 Hsp70 protein, and specific antiserum was generated. Two-dimensional Western blotting analysis showed that the purified antibodies were monospecific. Immunoelectron microscopy of developing and mature E. cuniculi spores revealed that the protein localized to internal structures and not to the spore surface. In spore adherence inhibition assays, the anti-C1 antibodies did not inhibit spore adherence to host cell surfaces, whereas antibodies to a known surface adhesin (EnP1) did so. In future studies, the antibodies to the ‘C1’ Hsp70 will be used to delineate spore surface protein expression.
Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi
Carrie E. Jolly,Cory A. Leonard,J. Russell Hayman
International Journal of Microbiology , 2010, DOI: 10.1155/2010/523654
Abstract: Microsporidia spore surface proteins are an important, under investigated aspect of spore/host cell attachment and infection. For comparison analysis of surface proteins, we required an antibody control specific for an intracellular protein. An endoplasmic reticulum-associated heat shock protein 70 family member (Hsp70; ECU02_0100; “C1”) was chosen for further analysis. DNA encoding the C1 hsp70 was amplified, cloned and used to heterologously express the C1 Hsp70 protein, and specific antiserum was generated. Two-dimensional Western blotting analysis showed that the purified antibodies were monospecific. Immunoelectron microscopy of developing and mature E. cuniculi spores revealed that the protein localized to internal structures and not to the spore surface. In spore adherence inhibition assays, the anti-C1 antibodies did not inhibit spore adherence to host cell surfaces, whereas antibodies to a known surface adhesin (EnP1) did so. In future studies, the antibodies to the ‘C1’ Hsp70 will be used to delineate spore surface protein expression. 1. Introduction Microsporidia are spore-forming, obligate intracellular divergent fungi with an extensive host range that includes most vertebrates and invertebrates. Although the first species of microsporidia was described over 150 years ago, microsporidiosis was rarely diagnosed in humans prior to the AIDS pandemic. Today, microsporidia are recognized as opportunistic pathogens of humans [1]. Most microsporidia infections in humans are thought to arise via the fecal-oral route. Ingestion of the environmentally stable spores leads to primary infection in the small intestine where replication of the organisms results in destruction of the epithelium. Therefore, the most common clinical manifestations of microsporidiosis are self-limiting diarrhea in immunocompetent individuals and persistent diarrhea perhaps leading to a wasting syndrome in the immunocompromised [2]. All microsporidia possess a unique invasion apparatus known as the polar tube or polar filament, which must be discharged in order to infect the host cell. Upon extrusion, the polar tube penetrates the host cell plasma membrane and allows the passage of infectious sporoplasm from the spore through the hollow polar tube into the host cell cytoplasm where replication occurs. We hypothesize that infection of the host cell is facilitated by adherence of the microsporidia spore to the host cell surface prior to or during the activation process. Our previous studies have demonstrated that microsporidia spores of the genus Encephalitozoon adhere to the
Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity
Cory A. Leonard,Stacy D. Brown,J. Russell Hayman
International Journal of Microbiology , 2013, DOI: 10.1155/2013/901697
Abstract: Multidrug-resistant bacteria cause severe infections in hospitals and communities. Development of new drugs to combat resistant microorganisms is needed. Natural products of microbial origin are the source of most currently available antibiotics. We hypothesized that random mutagenesis of Aspergillus oryzae would result in secretion of antibacterial compounds. To address this hypothesis, we developed a screen to identify individual A. oryzae mutants that inhibit the growth of Methicillin-resistant Staphylococcus aureus (MRSA) in vitro. To randomly generate A. oryzae mutant strains, spores were treated with ethyl methanesulfonate (EMS). Over 3000 EMS-treated A. oryzae cultures were tested in the screen, and one isolate, CAL220, exhibited altered morphology and antibacterial activity. Culture supernatant from this isolate showed antibacterial activity against Methicillin-sensitive Staphylococcus aureus, MRSA, and Pseudomonas aeruginosa, but not Klebsiella pneumonia or Proteus vulgaris. The results of this study support our hypothesis and suggest that the screen used is sufficient and appropriate to detect secreted antibacterial fungal compounds resulting from mutagenesis of A. oryzae. Because the genome of A. oryzae has been sequenced and systems are available for genetic transformation of this organism, targeted as well as random mutations may be introduced to facilitate the discovery of novel antibacterial compounds using this system. 1. Introduction Antibiotic resistant microorganisms became a medical concern shortly after antibiotics became readily available in the 1940s. In fact, resistance has typically been identified within four years of Food and Drug Administration approval of antibacterial agents [1]. Currently, multidrug-resistant bacteria such as MRSA, coagulase negative Staphylococci, and Enterococci cause severe infections in both hospital and community settings [2]. Development of new drugs to combat the increasing host of drug resistant microorganisms is essential if we are to avoid the emergence of pathogens for which there exist no effective antimicrobial therapies. Most classes of antibiotics were developed from natural products produced by fungi or filamentous bacteria. Significantly, the majority of antibiotics still commonly used today are natural product compounds, or their derivatives, discovered during the “golden era” of antibiotic discovery from the 1940s through the 1960s [3]. More recent efforts to generate new antibiotics based on high-throughput, target-focused screening of large libraries of synthetic compounds have largely
The Information standard, What It Is, How It Affects Librarianship, and Why It Has Been Overlooked
  Kenneth A. Cory
Journal of Library and Information Science , 1994,
Abstract: 頁次:1-12
The Presenting Symptom of Metastatic Prostate Carcinoma: Case of a Large Supraclavicular Mass and Review of Literature  [PDF]
Cory A. Vaughn, Kaitlin R. Jaqua, Ryan K. Meacham, Fransisco Vieira
International Journal of Otolaryngology and Head & Neck Surgery (IJOHNS) , 2013, DOI: 10.4236/ijohns.2013.25043
Abstract: Prostate cancer is the most common noncutaneous malignancy of American males and typically presents with genitourinary symptoms, however, head and neck extension is a rare finding. We present a case of a 53-year-old male initially presenting with a large supraclavicular mass as the only complaint. After an initial non-diagnostic FNA biopsy, PSA levels, histology and immunohistochemical findings were consistent with metastatic prostatic adenocarcinoma. The patient was started on anti-hormonal treatment after diagnosis. Due to the increasing reports of such cases, we suggest PSA levels and appropriate immunohistochemical staining should be obtained on all unknown neck masses.
A CONCEPTUAL FRAMEWORK FOR MANAGING RELATIONSHIPS BETWEEN ALL PARTICIPANTS DURING IT SERVICE AND SUPPORT ACTIVITIES
A.C. Leonard
South African Journal of Industrial Engineering , 2012,
Abstract: ENGLISH ABSTRACT: Since the early days of computing, IT professionals have been struggling with their end users (customers) to such an extent that end users became sceptic about the quality of service and support IT professionals can offer. As such, relationships between IT professionals and end users were in many cases very poor, which impacts negatively on the efforts to use information technology to the advantage of organizations or communities as a whole. This paper briefly describes the historical reasons therefor and gives a theoretical foundation for the establishment of IT-end user relationships. The paper describes IT-end user relationships as intriguing and complex and proposes a conceptual framework that explains all the important elements involved during the establishment and maintenance of sound relationships as well as for managing change. This paper is based on a research study conducted into the working relationship between IT departments and its end users. The research was done by means of a qualitative approach in which thought experiments were used to inductively refine the results of the research study. AFRIKAANSE OPSOMMING:Sedert die ontstaan van die rekenaarwese en die gepaardgaande dienslewering via inligtingstegnologie bestaan die neiging by eindpuntgebruikers om skepties te staan teenoor die gehalte van diens wat deur inligtingstegnolo gebied kan word. Dit het daartoe gelei dat die verhoudinge tussen eindpuntgebruikers en die inligtingstegnolo vertroebel is. Voordelige gebruik van inligtingstegnologie deur ondernemings en die gemeenskap is gevolglik daardeur benadeel. Hierdie stuk behandel kortliks die historiese oorsake vir die toedrag van sake. Dit beskryf ook die teoretiese grondslae vir die skepping van wedersydse verhoudings vir die probleemsituasie. Die stuk ondersoek die aandagwekkende en komplekse verhoudingsmilieu. Dit beskryf vervolgens ’n voorgestelde konsepraamwerk waarmee die belangrik elemente by die daarstelling en instandhouding van gesonde verhoudings blootgelê kan word. Dit behandel ook die gepaardgaande begrip van veranderingsbestuur. Die navorsing is gebaseer op werklike praktykgegewens. Die navorsings maak gebruik van kwalitatiewe metodes en induksie om die resultate tot bruikbaarheid te verfyn.
A New Silphid Beetle From A simple Insect—Trap
A. Leonard Melander
Psyche , 1902, DOI: 10.1155/1902/56126
Abstract:
Anton Chekhov’s ‘The Princess’: diagnosis – Narcissistic Personality Disorder
Leonard A. Polakiewicz
Australian Slavonic and East European Studies , 2008,
Abstract:
‘An Unpleasantness’: a rare case of violent protest in Chekhov’s works
Leonard A. Polakiewicz
Australian Slavonic and East European Studies , 2010,
Abstract:
Los topónimos de la Galicia romana: Nuevo estudio
Curchin, Leonard A.
Cuadernos de Estudios Gallegos , 2008,
Abstract: The study of place-names is important to the reconstruction of the linguistic layers that comprised ancient Galicia. In this paper we re-examine the etymology of the names of towns, rivers and other physical features preserved in sources of the Roman period. Conclusions are reached as to the relative importance of Celtic, Indoeuropean (non- Celtic) and Latin elements in the region’s toponymy. The largest group of toponyms turns out to be Celtic, representing about 40% of the total, followed closely by Indoeuropean. A low proportion of Latin names attests to the weakness of romanization in this region. El estudio de los topónimos es importante para reconstruir el sustrato lingüístico que abarcaba la antigua Galicia. En este ensayo reexaminamos la etimología de los nombres de pueblos, ríos y otros elementos del medio físico que se conservan en fuentes de época romana. Se obtienen conclusiones sobre la importancia relativa de los elementos celtas, indoeuropeos (no celtas) y latinos en la toponimia de la región. El grupo de topónimos más extenso es el celta, que representa cerca del 40% del total, seguido del indoeuropeo por un margen estrecho. La escasa proporción de nombres latinos atestigua el impacto reducido de la romanización en esta región.
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