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Search Results: 1 - 10 of 103 matches for " Coldebella Arlei "
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Atividade antibacteriana de desinfetantes para uso na produ??o organica de aves
Jaenisch, Fátima Regina Ferreira;Kuchiishi, Suzana Satomi;Coldebella, Arlei;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000200020
Abstract: efficient products in the disinfection that do not cause damages to the environment are a challenge for the organic poultry keeping. the antibacterial activity of four disinfectants was evaluated to per acetic acid, quaternary ammonium, sodium hypochlorite at 1% and 0,1% and the composed of organics acids (citric, lactic and ascorbic) against standard samples of escherichia coli, salmonella enteritidis and staphylococcus aureus in the presence and absence of organic matter, at two different temperatures and with 20 minutes of contact. organic acids were shown less effective in the presence of organic matter. however, the per acetic acid in the absence of this revealed most efficient against s. enteritidis and equally effective in the presence of organic matter, against s. aureus and e. coli showing a valid option for disinfection in the organic poultry keeping since preceded of careful cleanness.
Métodos de conserva??o de amostras de leite para determina??o da contagem bacteriana total por citometria de fluxo
Cassoli, Laerte Dagher;Machado, Paulo Fernando;Coldebella, Arlei;
Revista Brasileira de Zootecnia , 2010, DOI: 10.1590/S1516-35982010000200029
Abstract: the objective of this study was to evaluate the effect of storage temperature and sample age on the total bacterial count (tbc) of milk samples preserved with azidiol to verify whether a single sample could be used to perform regulatory milk analysis under the normative instruction 51 (in-51). three hundred and twenty milk aliquots were used collected from an expansion tank to assess three storage temperatures (0 oc - frozen: 7 oc - refrigerated, and 24 oc - room temperature), three types of conservation (bronopol, azidiol and no preservative) and four sample ages (1, 3, 5, and 7 days). for the total bacteria count, refrigerated control samples, with azidiol and one day old samples, and for composition and somatic cell count (scc), the controls were defined as one day old refrigerated samples containing bronopol. samples conserved with bronopol presented lower total bacteria count than the control regardless of storage temperature and sample age. milk samples conserved with azidiol and stored refrigerated presented lower scc, regardless of age, compared to refrigerated samples conserved with bronopol. the fat and lactose contents decreased after the fifth day. two samples had to be collected: one for scc determination and composition containing bronopol and another for the total bacteria count containing azidiol. milk samples used for total bacteria count can be tested up to 7 days if they are kept at 7 oc with the addition of azidiol.
Segments of competition in south brazilian wineries
Miele, Marcelo;Coldebella, Arlei;Waquil, Paulo Dabdab;Miele, Alberto;
Scientia Agricola , 2007, DOI: 10.1590/S0103-90162007000300003
Abstract: the international restructuring process in the beverage sector reached the winemaking business in the 1990s, changing its panorama. increasing competitive pressure is a characteristic of this period with significant impact in regions such as the countries belonging to the mercosul block. to support firm strategies and public policies, the goal of this study was to identify the competition segments in winemaking in rio grande do sul, the southern state of brazil, and in a specific form to propose a typology of wineries based on the industrial organization literature. the data used are from the state wineries data bank of rio grande do sul, which were submitted to multivariate statistical analysis, integrating a cluster and principal components analysis in a group of 381 observations. the results showed the existence of two factors, one related to scale and diversification, and the other related to the quality of the products and of the raw material. from these factors, six groups of wineries were identified that can be named segments of competition in winemaking. group 1 is characterized by quality and specialization; group 2, by a large production scale and diversification; group 3, by a low added value; group 4, by a micro production scale; and groups 5 and 6, by a low added value, small scale, and specialization.
Fatores associados à soroprevalência de Salmonella em rebanhos comerciais de suínos
Kich Jalusa Deon,Mores Nelson,Piffer Itamar Antonio,Coldebella Arlei
Ciência Rural , 2005,
Otimiza??o da técnica da PCR para a detec??o de Actinobacillus pleuropneumoniae
Souza, Karina Koerich de;Klein, Catia Silene;Kich, Jalusa Deon;Coldebella, Arlei;Alberton, Geraldo Camilo;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000800023
Abstract: the use of molecular methods based on pcr is important in actinobacillus pleuropneumoniae detection, being able to identify the infection before the establishment of the disease in the herd. these methods have larger sensitivity when compared with traditional methods of bacteriological isolation, but they can suffer influence of substances that reduce the specificity of the test and resulting in inespecific amplifications. in order to reduce inespecific amplifications, observed when applied the pcr technique for the gene cpx in tonsil's tissue samples, the optimization was performed, in which different annealing temperatures were analyzed and introduced, in the technique, an antibody that binds to the enzyme taq dna polimerase, increasing its specificity. in parallel, an experiment was performed in order to verify the inhibiting effect of the tonsil's tissue on the pcr results. for that, portions of tonsil from animals negative to the a. pleuropneumoniae were artificially contaminated with the reference sample of the sorotype 5b. the addition antibody for the enzyme taq dna polimerase and the increase of the primers anneling temperature to 57°c reduced the inespecific amplifications. the results obtained in the experiment demonstrated a possible inhibiting effect of the tonsil's tissue in the pcr amplifications. besides, amplifications depend on at least 675 ufc present in the aliquot of samples that will be used in pcr (equivalent to 1.35 x 105 ufc ml-1), therefore, samples tissue's fragments in initial infections and/or with few cells can result in false-negative.
Fatores associados à soroprevalência de Salmonella em rebanhos comerciais de suínos
Kich, Jalusa Deon;Mores, Nelson;Piffer, Itamar Antonio;Coldebella, Arlei;Amaral, Armando;Ramminger, Lucas;Cardoso, Marisa;
Ciência Rural , 2005, DOI: 10.1590/S0103-84782005000200024
Abstract: a cross-sectional study was conducted with 65 finishing pig herds from the states of rio grande do sul and santa catarina, brazil, in order to identify factors associated with seroprevalence for salmonella. pig farms were visited one week prior to slaughtering of animals when personnel were asked to answer a questionnaire. feed was also sampled for attempts of salmonella isolation, water for colimetrics analisis and around 40 pigs were bled. feed samples were subjected to salmonella isolation in selective media and sera were tested in an elisa, plates were coated with antigens of serovar typhimurium. herds were classified in one of three categories according to the prevalence of elisa positive sera, being low (less then 40% of positive sera), medium (between 40 and 70% positive sera) or high (more then 70% positive sera). seroprevalence was used as the explanatory variable and results obtained from the attempts to isolate salmonella from feed, water colimetrics results and the questionnaire answers were used as explanatory variables. initially, attempts of association between explanatory and explained variables were performed using the chi-square test. when associated (p£0.1), the two variables underwent multiple correspondence analysis. factors associated with herds having high seroprevalence were: in finishing herds, pelleted feed, swine manure disposal less than 100m from surface water, feeder not provided with water drinker, swine from several herds transported together to slaughterhouse; in the farrow-to-finish herds, feed ingridients exposure to other animals, no active rodent control, dry feed, absence of fence, whitewashing of facilities after cleaning and disinfecting and permission for other people entrance to the herd. among the 65 pig herds visited, 98.5% were elisa-positive, with seroprevalence of 57.6% (confidence interval 56 to 60%).
Indicadores do ambiente ruminal e suas rela??es com a composi??o do leite e células somáticas em diferentes períodos da primeira fase da lacta??o em vacas de alta produ??o
Campos, Rómulo;González, Félix;Coldebella, Arlei;Cardoso, Felipe;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000200025
Abstract: in the current international milk payment system, where the quality is the main factor; two fundamental aspects have acquired great importance: the somatic cells count and the amount of total solids. the first one depends on the health of the mammary gland and the second factor, which has more economic impact for the dairy industry, is directly related to feeding management and the cattle breed. the environment of the rumen can modify the composition of the milk, specially the fat percentage. the main source of protein in the ruminants depends on the synthesis of protein in the rumen. the current feeding systems used in high-yielding dairy cows, predispose them to the outcome of metabolic syndromes that directly or indirectly affect the milk production. the purpose of this study was to establish a relation between the index of the rumen environment (methylene blue reduction time and ph) and urinary ph with the composition of the milk and the somatic cells count. none statistical relation was found among the parameters evaluated. the average values of non fat solids in this study were below the official values approved in brazil.
Rea??o em Cadeia da Polimerase (PCR) baseada no gene cpx para detec??o de Actinobacillus pleuropneumoniae em suínos natural e experimentalmente infectados
Souza, Karina Koerich de;Klein, Catia Silene;Kich, Jalusa Deon;Coldebella, Arlei;Alberton, Geraldo Camilo;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000700024
Abstract: swine pleuropneumonia is one of the most important pig respiratory diseases and has been found in all producer countries. for control and monitoring of pleuropneumonia, it is necessary the development of fast and specific methods of diagnosis. to validate pcr based on the cpx gene of actinobacillus pleuropneumoniae in positive pigs, an experimental infection with a. pleuropneumoniae serotype 5b was performed and samples were obtained by tonsil swab, tonsil biopsy and blood for pcr, bacterial isolation and elisa, respectively. these tests were then performed in naturally infected pigs from three herds with different sanitary situations of clinical disease. in each herd, five groups of different ages were analyzed. tonsil biopsy for bacterial isolation and pcr and blood to determine the herd serological status was collected. the results obtained in the experimental infection confirmed that, even with the infection establishment, proved with bacterial isolation, it was not possible to detect the agent by pcr 45 days after infection. in naturally infected animals, pcr was more sensitive than bacterial isolation. the association between pcr and elisa is a good alternative to define the herd sanitary status regarding the infection with a. pleuropneumoniae.
Evaluation of litter material and ventilation systems in poultry production: III. litter reuse, darkling beetle populations and intestinal parasites
Abreu, Valéria Maria Nascimento;Paiva, Doralice Pedroso de;Abreu, Paulo Giovanni de;Coldebella, Arlei;
Revista Brasileira de Zootecnia , 2011, DOI: 10.1590/S1516-35982011000600028
Abstract: it was evaluated the quality of rice husks or soybean straw as litter substrate and the effect of litter reuse for four consecutive flocks of broiler chickens on populations of darkling beetle and intestinal parasite and as organic fertilizer. the experiment was carried out in four 12 m × 10 m poultry houses, internally divided in 4 boxes/poultry house with 200 birds/pen for four consecutive flocks, each flock with a duration of 42 days and with a 15-day downtime between flocks. the evaluated treatments were two ventilation systems (stationary or oscillating fans) and two litter materials (soybean straw or rice husks). darkling beetle population was followed by collecting the insects in traps, three traps per box, and endoparasite litter contamination was determined by eggs/oocyst counts per gram of litter. levels of dry matter, total nitrogen, total phosphorus, potassium, copper, zinc, manganese, iron, organic carbon and ph were evaluated by physical/chemical analyses of samples of the litters in each flock. overall, litters of broilers used by three flocks meet the minimal legal requirements to be marketed as simple organic fertilizer, regardless to the material used as substrate. soybean straw presented higher darkling beetle counts in stationary ventilation system as well as in oscillating ventilation system. the probability of rice husks litter present contamination by eimeria spp oocysts is 18.78 times higher in rice husks than in soybean straw litter when both are submitted to oscillating ventilation, and 1.32 higher when stationary ventilation is used. litter temperature does not influence significantly levels of contamination by oocysts.
Influência dos níveis de umidade na colheita e nas temperaturas de secagem de gr?os de trigo sobre desempenho, rendimento e composi??o de carca?a de frangos de corte
ávila, Valdir Silveira de;Mazzuco, Helenice;Portella, José Antonio;Ludke, Jorge Victor;Coldebella, Arlei;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000600013
Abstract: this study was conducted to evaluate the influence of humidity levels at harvesting period and drying temperatures of wheat grain in isoprotein, isoenergy and isovolumetric diets on broiler performance. the wheat grains were harvested with 30, 16 and 13% of humidity levels and submitted to drying temperatures of 100, 70 and 40°c. the level of 13% of humidity was not submitted to drying. the diets were offered to birds in three distinct periods, 1-21; 22-35 and 36-42 days of age. the parameters evaluated were body weight gain, feed:gain ratio, feed intake and weight gain. during the three evaluated periods the body weight gain and during 22-35 days and 36-42 days the feed intake of broilers submitted to diets with wheat were higher. feed:gain ratio of broilers submitted to wheat diets during the 1-21 days period were lower. at 21 and 42 days of age, one bird representing the average body weight of each replicate was selected for processing and evaluation of carcass nutrient composition and crude energy content, however there were no differences for these parameters at both ages. birds representing the average body weight of all replicates were processed for determination of carcass yield at 42 days of age. in absolute terms carcass weights were higher than birds under the treatment with wheat, in spite of humidity at harvest and the following drying temperature applied. inclusion of wheat in broiler diets in levels of 50 % in substitution of corn is technically feasible during all the raising period (1 42 days), in spite of humidity at harvest and drying temperature.
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