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Search Results: 1 - 10 of 6797 matches for " Ciacci-Zanella Janice Reis "
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Lack of evidence of porcine reproductive and respiratory syndrome virus (PRRSV) infection in domestic swine in Brazil
Ciacci-Zanella, Janice Reis;Trombetta, Cristiano;Vargas, Ildara;Costa, Denise Euclydes Mariano da;
Ciência Rural , 2004, DOI: 10.1590/S0103-84782004000200018
Abstract: this report describes the first prevalence of antibodies and experimental inoculation of suspected samples of porcine reproductive and respiratory syndrome virus (prrsv) from elisa positive pigs from swine herds in brazil. based on the hypothesis that this agent is present in swine herds worldwide, the objective of this work was to establish a diagnostic methodology and to investigate the occurrence of prrsv in brazilian swine herds. fifty-four swine herds, the total number which imported genetic material (live pigs or swine semen) from countries where prrs was endemic from 1990 to december 2000, from eight brazilian states all included in this study. the sampling used was such as to detect a prevalence of infection of 5%, with a confidence level of 95%. a total of 3785 serum samples were tested for prrsv antibodies by elisa. following the elisa test, which was performed with two different commercial kits, all serum positive pigs were retested, examined and additional materials were collected. viral isolation in permissive tissue culture cells and swine bioassays were performed. additionally, reverse transcriptase polymerase chain reaction (rt-pcr) and nested rt-pcr were also performed. we could not demonstrate the presence of prrsv or rna of prrsv by viral isolation or rt-pcr (or nested rt-pcr), respectively in all of the analyzed samples. furthermore, the pigs inoculated with prrsv suspicion samples did not seroconvert nor produce characteristic prrs lesions in the swine bioassay. thus, our results indicate no evidence of prrsv in the samples analyzed from swine herds in this study.
Identifica o do circovírus suíno tipo 2 por rea o em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil
Ciacci-Zanella Janice Reis,Morés Nelson,Simon Neide Lisiane,Oliveira Salete Rodrigues de
Ciência Rural , 2006,
Abstract: A síndrome multissistêmica do definhamento dos suínos (SMDS) é uma doen a de importancia econ mica causada pelo circovírus suíno tipo 2 (PCV2). Uma pesquisa retrospectiva foi realizada em amostras de órg os de suínos fixados em blocos de parafina arquivados, que haviam sido submetidos à Embrapa Suínos e Aves entre 1985 e 1998 para o diagnóstico histopatológico. Vinte e cinco casos foram selecionados com base nas les es histológicas características da SMDS, tais como linfoadenopatia, pneumonia intersticial, hepatite e nefrite intersticial. A presen a de PCV2 nos cortes histológicos foi pesquisada por rea o em cadeia da polimerase interna (nested-PCR), na qual utilizou-se primers específicos para a seqüência da ORF2 do PCV2 e também por imunoistoquímica, utilizando um anticorpo monoclonal específico para o capsídeo do PCV2. O DNA viral e os antígenos específicos do PCV2 foram detectados em amostras de tecidos de dois dos 25 casos analisados, sendo um desses datado de 1988. Esses resultados indicam que o PCV2 já estava presente no Brasil desde 1988.
Lack of evidence of porcine reproductive and respiratory syndrome virus (PRRSV) infection in domestic swine in Brazil
Ciacci-Zanella Janice Reis,Trombetta Cristiano,Vargas Ildara,Costa Denise Euclydes Mariano da
Ciência Rural , 2004,
Abstract: This report describes the first prevalence of antibodies and experimental inoculation of suspected samples of porcine reproductive and respiratory syndrome virus (PRRSV) from ELISA positive pigs from swine herds in Brazil. Based on the hypothesis that this agent is present in swine herds worldwide, the objective of this work was to establish a diagnostic methodology and to investigate the occurrence of PRRSV in Brazilian swine herds. Fifty-four swine herds, the total number which imported genetic material (live pigs or swine semen) from countries where PRRS was endemic from 1990 to December 2000, from eight Brazilian States all included in this study. The sampling used was such as to detect a prevalence of infection of 5%, with a confidence level of 95%. A total of 3785 serum samples were tested for PRRSV antibodies by ELISA. Following the ELISA test, which was performed with two different commercial kits, all serum positive pigs were retested, examined and additional materials were collected. Viral isolation in permissive tissue culture cells and swine bioassays were performed. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) and nested RT-PCR were also performed. We could not demonstrate the presence of PRRSV or RNA of PRRSV by viral isolation or RT-PCR (or nested RT-PCR), respectively in all of the analyzed samples. Furthermore, the pigs inoculated with PRRSV suspicion samples did not seroconvert nor produce characteristic PRRS lesions in the swine bioassay. Thus, our results indicate no evidence of PRRSV in the samples analyzed from swine herds in this study.
Identifica??o do circovírus suíno tipo 2 por rea??o em cadeia da polimerase e por imunoistoquímica em tecidos suínos arquivados desde 1988 no Brasil
Ciacci-Zanella, Janice Reis;Morés, Nelson;Simon, Neide Lisiane;Oliveira, Salete Rodrigues de;Gava, Danielle;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000500021
Abstract: postweaning multisystemic wasting syndrome (pmws) is of economical importance a disease caused by porcine circovirus type 2 (pcv2). a retrospective investigation was performed on paraffin-embedded organs samples from swine submitted to embrapa swine and poultry research center between 1985 and 1998 for histopathologic diagnosis. a total of 25 cases were chosen from the archival collection of the animal health laboratory at embrapa swine and poultry based on characteristic pathological lesions of pmws, such as lymphadenopathy, interstitial pneumonia, hepatitis and interstitial nephritis. the sections were investigated by nested-pcr (polymerase chain reaction) which used specific primers for the orf2 sequence of the pcv2 and by immunohistochemistry using a monoclonal antibody specific for pcv2 capsid antigen. virus specific dna and antigen were detected in tissue samples of two out of 25 analyzed cases. the earliest positive sample originated from 1988. these results indicate that pcv2 is present in brazil since 1988.
Erradica??o da doen?a de Aujeszky em Santa Catarina: importancia da condi??o sanitária das leitoas de reposi??o
Ciacci-Zanella, Janice Reis;Amaral, Armando Lopes do;Ventura, Lauren das Virgens;Morés, Nelson;Bortoluzzi, Hélio;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000300024
Abstract: aujeszky's disease (ad) is an herpesvirus infection, caused by the pseudorabies virus (prv), primarily in swine and present in santa catarina state (sc) since 1984. due to the impacts of ad in the pork export, breeder?s trade and productivity losses, an eradication program, financed by industry and swine producers association, has successfully eradicated the ad of swine herds in sc state. the last case of ad in the state was identified in july of 2004. during the depopulation/repopulation process, a prv positive swine herd located in the west region of sc state was detected. traceability studies of the origin of those animals indicated that the source was a swine farm which illegally distributed breeders without sanitary certification. this swine producer maintained an integration system which included 40 different producers, to whom were commercialized breeders and/or finishers. serum-prevalence tests detected prv antibodies in 12 of those herds. due to the location into the 2.5km of radius from the initial outbreak, another swine farm, which had an artificial insemination center that distributed swine semen to another 5 herds of the same owner was tested positive as well. the objectives of this paper are to describe the sanitary status related to ad on the farms which have received pigs or swine semen from these swine producers, the measures to control and eliminate adv from positive herds and the outcome of this work. beyond that, to alert that measures of active surveillance and sanitary rules for commerce and distribution of genetic materials must be properly fulfilled, otherwise, ad can reactivate and become out of control as occurred before the eradication program.
Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system
Dambros, Régia Maria Feltrin;Ribeiro, Bergman Moraes;Aguiar, Raimundo Wagner de S.;Schaefer, Rejane;Esteves, Paulo Augusto;Perecmanis, Simone;Simon, Neide Lisiane;Silva, Nayara Cavalcante;Coldebella, Michele;Ciacci-Zanella, Janice Reis;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000300021
Abstract: aujeszky' s disease (ad) is an infectious disease causing important economic losses to the swine industry worldwide. the disease is caused by an alpha-herpesvirus, aujeszky' s disease virus (adv), an enveloped virus with a double stranded linear dna genome. the adv genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to the infection. the glycoprotein e (ge) is a non-essential protein and deletion of the ge gene has been used for the production of marker vaccines. aiming to develop molecular reagents for the production of a ge specific elisa test, the ge gene was amplified by pcr, cloned and expressed into a baculovirus expression system. the recombinant dna vector pfastbac-ge-adv was used for site-specific transposition into the recombinant baculovirus (bacmid). colonies with recombinant bacmid-pfastbac-ge-adv were selected by antibiotic and color selection and the presence of the ge gene was confirmed by pcr. the recombinant bacmid-pfastbac-ge-adv was cotransfected in insect trichoplusia ni and the presence of the recombinant dna and ge protein were detected by pcr, sds-page and western blotting, respectively.
Diagnóstico e genotipagem do vírus da pseudoraiva por nested-PCR e análise de restri??o enzimática
Fonseca Júnior, Ant?nio Augusto;Carmagos, Marcelo Fernandes;D'Ambros, Régia Maria Feltrin;Braga, Alexandre Carvalho;Ciacci-Zanella, Janice;Heinemann, Marcos Bryana;Leite, R?mulo Cerqueira;Reis, Jenner Karlisson Pimenta dos;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010005000066
Abstract: pseudorabies is a disease caused by suid herpesvirus 1 (prv) and is responsible for considerable economic losses in the swine industry. the prv has only one serotype, but based on rflp (restriction fragment length polymorphism) the virus was divided into four genotypes named i, ii, iii, iv. the classical methods for prv genotyping usually require virus isolation, dna purification enzyme restriction analysis and a long electrophoresis. the aim of this research was to describe a faster and more sensitive method to detect and genotype prv based on nested-pcr and restriction enzyme analysis. twenty prv isolates from south and southeast regions of brazil, and the standard strain shope were grown in pk-15 cells and submitted to pcr for glycoprotein e gene amplification. additionally were tested 75 clinical samples (swine brain), with 25 positives for virus isolation and seroneutralization, and 50 negatives from a flock free pr with negative results in seroneutralization test. there was 100% of agreement between results of nested-pcr and virus isolation and seroneutralization and all samples detected were classified as genotype ii. the nested-pcr, combined with restriction enzyme analysis, was able to detect and genotype prv in 1-2 days with a sensitivity of 10-1,3 tcid50 ml-1. it was faster than classical methods described in the literature that require at least 7 days to be completed.
Transmission of porcine circovirus 2 (PCV2) by semen and viral distribution in different piglet tissues
Gava, Danielle;Zanella, Eraldo L.;Morés, Nelson;Ciacci-Zanella, Janice R.;
Pesquisa Veterinária Brasileira , 2008, DOI: 10.1590/S0100-736X2008000100011
Abstract: porcine circovirus infections are caused by the porcine circovirus 2 (pcv2). among six different clinical manifestations involving respiratory, enteric, nervous and reproductive signs, the postweaning multisystemic wasting syndrome (pmws) is the most important and studied disease. however, reproductive failures associated with pcv2 have been increasingly reported. some studies have shown the possible contamination of sows by semen of pcv2 positive boars. in order to investigate the transmission of pcv2 by contaminated semen and its ability to infect the sow and piglets, 20 pcv2 negative sows were inseminated, 10 with negative boar semen and 10 with previously nested-pcr tested positive boar semen. the sows were weekly monitored and blood samples were collected. based on the results, 4 out 20 sows were selected (1 sow was pcr negative and inseminated with a negative semen, 2 sows were pcr negative and inseminated with a positive semen and 1 sow was pcr negative and inseminated with a positive semen, but became pcr positive around the 30 days of pregnancy). after weaning, 12 male piglets, 3 of each sow, were selected and maintained under isolation. in order to investigate which organs harbored the virus, the young pigs were necropsied around 9 months of age. samples of serum collected monthly were tested by immunocitochemistry (icc), and all 12 pigs serum converted. samples of lymphoid, systemic and reproductive organs were analyzed by nested-pcr and immunohistochemistry (ihc). evaluation of the samples by nested-pcr, revealed that several tissues were positive in 10 of 12 pigs, mainly the lymph nodes, bone marrow and spleen. various samples were positive by ihc in 8 of 12 piglets, being the lymph nodes, tonsils and bulbourethral glands the most frequently positive. thus, the results of testing different samples, in the 3 tests (icc, nested-pcr and ihc) were complementary. these results show that pcv2 transmission through semen to the sows and piglets may occur and ma
Absence of 2009 Pandemic H1N1 Influenza A Virus in Fresh Pork
Amy L. Vincent,Kelly M. Lager,Michelle Harland,Alessio Lorusso,Eraldo Zanella,Janice R. Ciacci-Zanella,Marcus E. Kehrli Jr.,Alexander Klimov
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008367
Abstract: The emergence of the pandemic 2009 H1N1 influenza A virus in humans and subsequent discovery that it was of swine influenza virus lineages raised concern over the safety of pork. Pigs experimentally infected with pandemic 2009 H1N1 influenza A virus developed respiratory disease; however, there was no evidence for systemic disease to suggest that pork from pigs infected with H1N1 influenza would contain infectious virus. These findings support the WHO recommendation that pork harvested from pandemic influenza A H1N1 infected swine is safe to consume when following standard meat hygiene practices.
Interstitial nephritis of slaughtered pigs in the State of Mato Grosso, Brazil
Oliveira Filho, Jo?o X.;Paula, Daphine A.J. de;Morés, Nelson;Pescador, Caroline A.;Ciacci-Zanella, Janice R.;Coldebella, Arlei;Dutra, Valéria;Nakazato, Luciano;
Pesquisa Veterinária Brasileira , 2012, DOI: 10.1590/S0100-736X2012000400007
Abstract: this study evaluated histological lesions in kidney samples from pigs with nephritis in two slaughterhouses in the state of mato grosso, brazil. four hundred samples were subjected to histology, anti-porcine circovirus type 2 (pcv2) immunohistochemistry (ihc), anti-leptospira sp. immunofluorescence (if), and polymerase chain reaction (pcr) for pcv2, porcine parvovirus (ppv), and torque teno virus type 1 and 2 (ttv1, ttv2) detection. histological lesions were found in 81% of the samples, and mononuclear interstitial nephritis was the most frequent lesion (77.50%). a follicular pattern was observed in 40.97% of the interstitial nephritis lesions. pcv2, ppv, ttv1, and ttv2 were identified in the kidneys by pcr in 27.25%, 28.50%, 94%, and 87.5% of the samples, respectively. leptospira sp. was not detected through if. infection by pcv2 (pcr) and the presence of histological lesions (p=0.008) and giant cells (p=0.0016) were significantly associated. an association was observed between the ttv2-ttv1 co-infection (p<0.0001) and the risk for pathogenesis. these findings indicated that pcv2, ppv, ttv1, and ttv2 were widely distributed among pigs in the local farms and that the presence of these agents should be considered in the differential diagnosis of kidneys with interstitial nephritis in pigs.
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