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Multiplex PCR for simultaneous detection of Streptococcus agalactiae, Streptococcus iniae and Lactococcus garvieae: a case of S. agalactiae infection in cultured Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis niloticus x Oreochromis mossambicus)
Akkarawit Itsaro,Naraid Suanyuk,Chutima Tantikitti
Songklanakarin Journal of Science and Technology , 2012,
Abstract: A multiplex PCR (m-PCR) technique was developed for simultaneous detection of the causative agents responsible forstreptococcosis of cultured fish in Thailand i.e., Streptococcus agalactiae, Streptococcus iniae, and Lactococcus garvieae.The study on the sensitivity of the technique indicated that the minimum detected DNA concentration was 9.76, 39.06, and19.53 pg for S. agalactiae, S. iniae and L. garvieae, respectively. Detection of streptococcosis in healthy and diseased Niletilapia (Oreochromis niloticus) and red tilapia (Oreochromis niloticus x Oreochromis mossambicus) cultured in Paphayomand Bangkaew District, Phatthalung Province and Sichon and Hua Sai District, Nakhon Si Thammarat Province, Thailand, bym-PCR technique showed positive results for S. agalactiae from tilapia cultured in Bangkaew and Hua Sai and negativeresults for samples from Paphayom and Sichon. The m-PCR results were in accordance with microbiological culture techniques,which detected S. agalactiae from tilapia cultured in Bangkaew and Hua Sai indicating that our m-PCR assay is a sensitiveand specific diagnostic tool for simultaneous detection of streptococcosis caused by S. agalactiae, S. iniae and L. garvieaein cultured fish in Thailand.
Effect of hemoglobin powder substituted for fishmeal on growth performance, protein digestibility, and trypsin gene expression in Litopenaeus vannamei
Duangrat Chookird,Chutima Tantikitti,Amornrat Pongdara,Manee Srichanun
Songklanakarin Journal of Science and Technology , 2010,
Abstract: Recent increases in the price and demand of fishmeal, the primary protein source in shrimp feeds, have caused asearch for alternative protein sources. Hemoglobin powder (HE) is a by-product produced by separating hemoglobin fromplasma of farm animal un-coagulant blood. HE contains high protein content but low lipid content, and thus has highpotential for fishmeal substitution.A six week feeding trial was carried out to investigate effects of HE substituted for fishmeal protein on growth performance,protein digestibility and trypsin gene expression in Pacific white shrimp (Litopenaeus vannamei). Six diets with0%, 12.5%, 25%, 50%, 75% and 100% of HE replacing fishmeal protein were fed four times daily to six groups of shrimp withan average initial weight of 3.53 g/shrimp. Growth of shrimp decreased with increasing level of HE substitution. Although the12.5% HE substitution caused significantly lower final weight, weight gain, SGR, feed intake, PER and PPV in comparisonwith the control diet, FCR of this diet was not statistically different (p<0.05).The activity of trypsin of shrimp was similar among the groups fed diets with HE substitution not higher than 50%(p>0.05). In-vitro and in-vivo protein digestibility of 12.5% HE substitution were significantly lower than that of the controlgroup in which the trypsin gene expression of shrimp fed 12.5% HE substituted diet was the highest.
Diplomonad flagellates of some ornamental fish cultured in Thailand
Suchanya Mankhakhet,Naraid Suanyuk,Chutima Tantikitti,Wutiporn Phromkunthong
Songklanakarin Journal of Science and Technology , 2012,
Abstract: The study on diplomonad flagellates infection in some ornamental fishes in the family cichlidae i.e., angelfish(Pterophyllum scalare), oscar (Astronotus ocellatus), blue mbuna (Labeotropheus fuelleborni) and the family osphronemidaei.e., Siamese fighting fish (Betta splendens) revealed that this parasite infected three out of four ornamental fish species,angelfish, oscar and blue mbuna. The highest infection was recorded in angelfish (90%) followed by oscar (75.4%) and bluembuna (61%), respectively. Identification of diplomonad flagellates from angelfish by means of morphological studies underlight and electron microscopes indicated that the parasite was Spironucleus vortens. The 14–days LD50 of S. vortens inangelfish was 2.99x103 cells. Histopathological changes of infected angelfish revealed granulomatous liver, numerousnumbers of melanomacrophage in the spleen and inflammation of the intestine. Susceptibility study of S. vortens to goldfish(Carassius auratus), guppy (Poecilia reticulata) and platy (Xiphophorus maculatus) indicated that they were resistant toartificial infection. In vitro examination of the growth inhibition assay of S. vortens indicated that dimetridazole and metronidazolewere effective in inhibiting parasite growth after 48 hrs exposure at concentrations of >4.0 μg/ml and >6.0 μg/ml,respectively. Magnesium sulfate at a concentration of >60 mg/ml inhibited the parasite growth after 72 hrs exposure. In vivoexamination of the dimetridazole efficiency on S. vortens infection indicated that dimetridazole at 4.0 μg/ml provided thehighest efficiency which could be used for treatment of spironucleosis in angelfish.
Digestive enzyme activity during ontogenetic development and effect of live feed in green catfish larvae (Mystus nemurus Cuv. & Val.)
Manee Srichanun,Chutima Tantikitti,Vichai Vatanakul,Poramet Musikarune
Songklanakarin Journal of Science and Technology , 2012,
Abstract: Mystus nemurus is one of the economically important freshwater species fish for aquaculture in Thailand. The aim ofthis study was to describe the development of proteolytic enzymes (pepsin, trypsin and chymotrypsin), carbohydrase ( -amylase) and lipase, and the influence of live feed during early stage of larval development. Enzymatic assays were conductedfrom day 1 to day 45 after hatching in larvae fed the following feed sequence: Moina sp. during 3-15 day after hatching(DAH), co-feeding between Moina sp. and powder feed during 16-29 DAH and artificial diet during 30-45 DAH. Threesamples of larvae were collected before morning feeding on days 1, 3, 5, 7, 9, 12, 15, 20, 25, 30, 35, 40 and 45. All enzymeswere detected at low level as early as hatching and remained constant until 20 DAH. Significant increases of pepsin, trypsin,chymotrypsin and -amylase activity were detected during 25-45 DAH (p<0.05) whereas lipase activity showed an increaseduring 30-45 DAH, which was related to an increased growth of fish larvae at 30 DAH onward (p<0.05). Live feed, Moina sp.,significantly increased enzyme activity of trypsin, chymotrypsin and -amylase of fish larvae during 3-15 DAH in comparison with those of unfed fish larvae (p<0.05).
Mixed-Model U-Shaped Assembly Line Balancing Problems with Coincidence Memetic Algorithm  [PDF]
Parames Chutima, Panuwat Olanviwatchai
Journal of Software Engineering and Applications (JSEA) , 2010, DOI: 10.4236/jsea.2010.34040
Abstract: Mixed-model U-shaped assembly line balancing problems (MMUALBP) is known to be NP-hard resulting in it being nearly impossible to obtain an optimal solution for practical problems with deterministic algorithms. This paper pre-sents a new evolutionary method called combinatorial optimisation with coincidence algorithm (COIN) being applied to Type I problems of MMUALBP in a just-in-time production system. Three objectives are simultaneously considered; minimum number workstations, minimum work relatedness, and minimum workload smoothness. The variances of COIN are also proposed, i.e. CNSGA II, and COIN-MA. COIN and its variances are tested against a well-known algo-rithm namely non-dominated sorting genetic algorithm II (NSGA II) and MNSGA II (a memetic version of NSGA II). Experimental results showed that COIN outperformed NSGA II. In addition, although COIN-MA uses a marginal CPU time than CNSGA II, its other performances are dominated.
Estimating the Demand for a Preventive HIV Vaccine: Why We Need to Do Better
Robert Hecht ,Chutima Suraratdecha
PLOS Medicine , 2006, DOI: 10.1371/journal.pmed.0030398
Network Design for Quality of Services in Wireless Local Area Networks: a Cross-layer Approach for Optimal Access Point Placement and Frequency Channel Assignment
Chutima Prommak,Boriboon Deeka
Lecture Notes in Engineering and Computer Science , 2007,
Simultaneous HPLC method for determination of sodium trimethoprim phenylpropanol disulphonate and sodium sulfaquinoxaline in veterinary drugs
Puangkaew Lakkanatinaporn,Chutima Matayatsuk
Songklanakarin Journal of Science and Technology , 2004,
Abstract: A simple HPLC method has been developed for the separation and determination of sodium trimethoprim phenylpropanol disulphonate and sodium sulfaquinoxaline in veterinary preparations. Both drugs were separated well on a Kromasil C18 column (5 μm, 150 × 4.6 mm) using a mixture of acetonitrile and 0.5% triethylamine in 1% acetic acid, pH 3 (18:82, v/v) as the mobile phase at the flow rate of 1.5 ml/ min. The presence of both substances was monitored by UV absorption detection at 271 nm. The retention times of sodium trimethoprim phenylpropanol disulphonate and sodium sulfaquinoxaline were 3.2 and 16.0 min, respectively. The performance of the developed method was tested. Linear responses of both drugs were achieved between 48-145% of labeled amount over the concentration ranges of 35-101 μg/ml and 102-306 μg/ ml for sodium trimethoprim phenylpropanol disulphonate and sodium sulfaquinoxaline with correlation coefficients (R2) of 0.9980 and 0.9998, respectively. Accuracy expressed in term of recoveries were 101.4± 1.21% (n=6) for sodium trimethoprim phenylpropanol disulphonate and 99.7±0.92% (n=6) for sodium sulfaquinoxaline. Precision of the method in terms of the relative standard deviation is not more than 2% in all cases. These figures of merit indicated the validity of the developed method.
Network reliability analysis for 3G cellular topology design
Chutima Prommak,Ekkaluk Eksook
Songklanakarin Journal of Science and Technology , 2010,
Abstract: Network reliability is an important issue in the topological design of the 3G cellular networks in order to guaranteeconsistent services. This paper aims to present network reliability analysis of various network topologies, including spanning tree, single ring, multiring, and two versions of hybrid ring-and-tree. We derived reliability analysis models for the hybrid ring-and-tree topologies in terms of the operational probability of the network links. The preliminary results show that the network reliability of the hybrid multiring-and-tree topologies is 116.78% and 13.78% higher than that of the spanning tree and the single ring topology, respectively, while it is only about 37.3% less than that of the pure multiring topologies.
Cross-layer Network Design for Quality of Services in Wireless Local Area Networks: Optimal Access Point Placement and Frequency Channel Assign
Chutima Prommak,Boriboon Deeka
IAENG International Journal of Computer Science , 2008,
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