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Search Results: 1 - 10 of 6770 matches for " Christina Thereza Rachel Bottari "
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Biblioteca digital de teses e disserta es da UERJ: desafios e oportunidades Biblioteca digital de tesis y disertaciones de la UERJ: desafíos y oportunidades.
Christina Thereza Rachel Bottari,Neusa Cardim da Silva
Informa??o & Informa??o , 2011, DOI: 10.5433/1981-8920.2011v16n1p88-101
Abstract: Aborda a importancia das bibliotecas digitais, especificamente de teses e disserta es, no contexto da dissemina o da informa o produzida pelas comunidades acadêmicas. Tra a um panorama dos Programas de Pós-Gradua o oferecidos na Universidade do Estado do Rio de Janeiro (UERJ). Destaca o papel do IBICT como idealizador e coordenador do Projeto da Biblioteca Digital Nacional de Teses e Disserta es (BDTD) e os procedimentos estabelecidos para a ades o das Institui es de Ensino Superior (IES), como participantes desse projeto. Cita o Sistema TEDE, customizado e distribuído gratuitamente pelo IBICT, com a finalidade de apoiar as IES na implanta o de suas BDTDs. Descreve as etapas do processo de implanta o da BDTD-UERJ, bem como os obstáculos e desafios enfrentados. Finaliza destacando os benefícios obtidos, as perspectivas futuras de cria o de novos produtos e servi os e da avalia o dos métodos e técnicas empregados até o momento.
Esteriliza o de artigos em unidades de saúde
Thereza Christina Vessoni Penna
Revista Brasileira de Ciências Farmacêuticas , 2003, DOI: 10.1590/s1516-93322003000300015
Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfpuv) from Escherichia coli
Thereza Christina Vessoni Penna, Marina Ishii
BMC Biotechnology , 2002, DOI: 10.1186/1472-6750-2-7
Abstract: Cultures (37°C/100 rpm/ 24 h; μ = 0.99 h-1 - 1.10 h-1) of transformed (pGFP) Escherichia coli DH5-α, expressing the green fluorescent protein (gfpuv, absorbance at 394 nm and emission at 509 nm) were sonicated in successive intervals of sonication (25 vibrations/pulse) to determine the maximum amount of gfpuv released from the cells. For selective permeation, the transformed previously frozen (-75°C) cells were subjected to three freeze/thaw (-20°C/ 0.83°C/min) cycles interlaid by sonication (3 pulses/ 6 seconds/ 25 vibrations). The intracellular permeate with gfpuv in extraction buffer (TE) solution (25 mM Tris-HCl, pH 8.0, 1 mM β-mercaptoethanol β-ME, 0.1 mM PMSF) was subjected to the three-phase partitioning (TPP) method with t-butanol and 1.6 M ammonium sulfate. Sonication efficiency was verified on the application to the cells previously treated by the TPP method. The intra-cell releases were mixed and eluted through methyl HIC column with a buffer solution (10 mM Tris-HCl, 10 mM EDTA, pH 8.0).The sonication maximum released amount obtained from the cells was 327.67 μg gfpuv/mL (20.73 μg gfpuv/mg total proteins – BSA), after 9 min of treatment. Through the selective permeation by three repeated freezing/thawing/sonication cycles applied to the cells, a close content of 241.19 μg gfpuv/mL (29.74 μg gfpuv/mg BSA) was obtained. The specific mass range of gfpuv released from the same cultures, by the three-phase partitioning (TPP) method, in relation to total proteins, was higher, between 107.28 μg/mg and 135.10 μg/mg.The selective permeation of gfpuv by freezing/thawing/sonication followed by TPP separation method was equivalent to the amount of gfpuv extracted from the cells directly by TPP; although selective permeation extracts showed better elution through the HIC column.The fluorescent green protein, gfp extracted from Aequorea victoria shows a maximum excitation under ultraviolet light (UV) in the interval of λ = 395–498 nm and emission in the interval of λ
Processo decisório e práticas de gest?o: dirigindo a Secretaria da Saúde do Estado da Bahia, Brasil
Coelho, Thereza Christina Bahia;Paim, Jairnilson Silva;
Cadernos de Saúde Pública , 2005, DOI: 10.1590/S0102-311X2005000500009
Abstract: this case study is based on research performed by the bahia state health secretariat (sesab), aimed at analyzing management practices during the implementation of a state government administrative reform. the institutional agenda shows evidence of limited participation by civil society and technical and operational staff in problem selection and prioritization, resulting from a work process pressured by high-level executive government staff. decisions regarding "output" (projects, services, and activities) were made under the responsibility of subordinate operational levels and did not appear as issues in the institutional routine. concerns related to "input" (financial and human resources) consumed most of the high-level efforts, with internal and external negotiations and compromises to assure access to them. meanwhile the possible "outcome", namely public health status, represents the "occult subject" of the institutional discourse. information emerges in institutional disputes as a technical "power resource" in its medical, epidemiological, health, and administrative dimensions. the issue of government "representation" and allied interests is based on ideological dispositions and authoritarian practices, thus contradicting the need for management transparency and modernization.
Mercado de trabalho: revendo conceitos e aproximando o campo da saúde. A década de 90 em destaque
Varella, Thereza Christina;Pierantoni, Célia Regina;
Physis: Revista de Saúde Coletiva , 2008, DOI: 10.1590/S0103-73312008000300009
Abstract: this paper analyzes health labor market in comparison with brazilian labor market in general. it first presents a theoretical study on the subject, to support debate. the characteristics of the labor market in general are discussed, in an effort to evince if the current stage of lack of structuring measures and of regulation can, in part, be reflected on the configuration of the health sector. it evinces a migratory movement of public jobs from the federal sphere to the municipal one, and shows that, in the 1990's, the health public sector made use of more flexible modalities to recruit health professionals.
Extra??o, purifica??o e caracteriza??o físico-química da proteína verde fluorescente recombinante (GFPuv) expressa em Escherichia coli
Chiarini, Eb;Penna, Thereza Christina Vessoni;
Revista Brasileira de Ciências Farmacêuticas , 2003, DOI: 10.1590/S1516-93322003000400014
Abstract: the recombinant green fluorescent protein (gfpuv) has been used as a marker in several research fields. the purpose of the present work was to evaluate the influence of the selective physical permeation procedure applied to the cells of escherichia coli for the extraction of gfpuv in relation to the chemical procedures of extraction and purification. transformed cells (0.92-1.44 mg/ml) of e. coli dh5-a expressing gfpuv were submitted to four cycles (1o, 2o, 3o, 4o) of freezing (-20 oc/ 0.83 oc/ min/thawing interlaid by sonication (3 pulses/6 s/25 vibrations). the intracellular permeate with gfpuv in buffer solution (tris-hcl 25 mm ph 8.0 + b-mercaptoethanol (1 mm) + pmsf (0.1 mm)) was submitted to the three-phase partitioning (tpp) method and subsequent purification through hydrophobic interaction chromatography column (hic). the results showed that the first selective permeation cycle applied to the cells was more efficient in the release of the protein from the cell fragments without subsequent extraction and purification in relation to the second, third and fourth permeation procedures. the permeated, extracted and purified gfpuv showed similarity to the standard gfpuv characteristics of fluorescence, stability and solubility.
A migra??o de enfermeiros: um problema de saúde pública
Varella, Thereza Christina;Pierantoni, Célia Regina;
Revista Brasileira de Saúde Materno Infantil , 2007, DOI: 10.1590/S1519-38292007000200011
Abstract: this article analyzes the international scenario of nurses' migration, an essential professional class in the organization of national health systems, focusing specially on the unbalance between central and peripheral countries. it depicts studies results performed by a department of the australian government concerning nurses, qualifying and quantifying their insertion in the labor market, as well as suggesting measures to reintegrate the nurses leaving the labor market, pointing out the reasons for the lack of nurses in developed countries. it highlights the expressive growth of nursing schools in brazil commenting on the possible consequences of the offer of this professional in the labor market establishing a parallel with the international situation.
Alexandre Pessoa Dias,Thereza Christina Almeida Rosso
Engevista , 2011,
Abstract: This paper examines the issue of the interconnectivity between sanitary and rain drainage systems and its impacts on water collections of City of Rio de Janeiro, with the extent to which type of system formally designed - separated sewer - in fact contains their health and environmental functions of collection, transport and adequate sewage disposal. It is discussed the hypothesis that many of the solutions adopted for cleaning water bodies gradually weak and disqualifies the advantages of this system, indicating the need for changing direction and trajectory to pollution control interventions in Rio de Janeiro by an urgent matter of environmental and social responsibility.
Processo decisório e práticas de gest o: dirigindo a Secretaria da Saúde do Estado da Bahia, Brasil
Coelho Thereza Christina Bahia,Paim Jairnilson Silva
Cadernos de Saúde Pública , 2005,
Abstract: Um estudo de caso da Secretaria da Saúde do Estado da Bahia, Brasil, identificou e analisou as práticas de gest o durante a implanta o de uma reforma administrativa. A forma o da "agenda" institucional apresentou indícios de pouca participa o da sociedade civil e do nível técnico-operacional na sele o e prioriza o de problemas, resultante de um processo decisório pressionado por instancias do alto staff do Executivo. As decis es relativas aos produtos finais (output - projetos, servi os e a es) correm por conta dos níveis subordinados, sendo pouco problematizadas na rotina institucional. A preocupa o com os insumos (input - recursos humanos e financeiros) ocupam grande parte do tempo dos dirigentes em negocia es internas e compromissos externos para assegurá-los. Já os resultados (outcome) em rela o à situa o de saúde representam o "sujeito oculto" do discurso institucional. A informa o surge nas disputas institucionais como "recurso de poder" técnico nas suas dimens es médica, epidemiológica, sanitária ou administrativa. A quest o da "representa o" de governo e dos interesses aliados se apóia em disposi es ideológicas e práticas autoritárias, contradizendo a necessidade de transparência e moderniza o gerencial.
Distribui??o da for?a de trabalho: eqüidade e negocia??o
Miguelote, Vera Regina da Silva;Fortes, Maria Thereza;Fagundes, Marise;Varella, Thereza Christina;
Physis: Revista de Saúde Coletiva , 2008, DOI: 10.1590/S0103-73312008000200007
Abstract: the significant existing disproportion in the density of human resources makes the equity of human resources distribution a strategic issue for possible solutions of health problems. this can explain the importance of the human resources issue in the global health agenda and its priority for the health sector. the policy of recruitment of health workers by developed countries as well as a lack of clear policies to prevent brain drain by developing countries are contributing migration factors. under the leadership of who, some international agencies have been developing and subsidizing human health resources policies. however the existing paradox between the interdependency and sovereignty of countries requires an ethical and strategic standpoint in order to strengthen nations in their commercial dealings. therefore one of the challenges to global health is in grabbing the opportunities offered by gats for international human health resource negotiations.
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