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Search Results: 1 - 10 of 414589 matches for " Christian Mühl "
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IFN-gamma Impairs Release of IL-8 by IL-1beta-stimulated A549 Lung Carcinoma Cells
Kim A Boost, Christian D Sadik, Malte Bachmann, Bernhard Zwissler, Josef Pfeilschifter, Heiko Mühl
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-265
Abstract: A549 cells were cultured and stimulated with interleukin (IL)-1β alone or in combination with IFNγ. IL-8 production by these cells was analyzed with enzyme linked immuno sorbent assay (ELISA). mRNA-expression was analyzed by real-time PCR and RNase protection assay (RPA), respectively. Expression of inhibitor-κ Bα, cellular IL-8, and cyclooxygenase-2 was analyzed by Western blot analysis.Here we demonstrate that IFNγ efficiently reduced IL-8 secretion under the influence of IL-1β. Surprisingly, real-time PCR analysis and RPA revealed that the inhibitory effect of IFNγ on IL-8 was not associated with significant changes in mRNA levels. These observations concurred with lack of a modulatory activity of IFNγ on IL-1β-induced NF-κB activation as assessed by cellular IκB levels. Moreover, analysis of intracellular IL-8 suggests that IFNγ modulated IL-8 secretion by action on the posttranslational level. In contrast to IL-8, IL-1β-induced cyclooxygenase-2 expression and release of IL-6 were not affected by IFNγ indicating that modulation of IL-1β action by this cytokine displays specificity.Data presented herein agree with an angiostatic role of IFNγ as seen in rodent models of solid tumors and suggest that increasing T helper type 1 (Th1)-like functions in lung cancer patients e.g. by local delivery of IFNγ may mediate therapeutic benefit via mechanisms that potentially include modulation of pro-angiogenic IL-8.Interleukin (IL)-1β is a cytokine with a key role in the pathophysiology of local and systemic inflammation [1]. Moreover, owing to its pro-inflammatory nature, IL-1β is regarded a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis has been observed under the influence of IL-1β [2,3]. Accordingly, IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β as observed in those studies was associated with increased activity of matrix metalloproteinases and expre
A single ion coupled to an optical fiber cavity
Matthias Steiner,Hendrik M. Meyer,Christian Deutsch,Jakob Reichel,Michael K?hl
Physics , 2012, DOI: 10.1103/PhysRevLett.110.043003
Abstract: We present the realization of a combined trapped-ion and optical cavity system, in which a single Yb^+ ion is confined by a micron-scale ion trap inside a 230 mum-long optical fiber cavity. We characterize the spatial ion-cavity coupling and measure the ion-cavity coupling strength using a cavity-stimulated Lambda-transition. Owing to the small mode volume of the fiber resonator, the coherent coupling strength between the ion and a single photon exceeds the natural decay rate of the dipole moment. This system can be integrated into ion-photon quantum networks and is a step towards cavity quantum-electrodynamics (cavity-QED) based quantum information processing with trapped ions.
Review of the Use of Electroencephalography as an Evaluation Method for Human-Computer Interaction
Jérémy Frey,Christian Mühl,Fabien Lotte,Martin Hachet
Computer Science , 2013,
Abstract: Evaluating human-computer interaction is essential as a broadening population uses machines, sometimes in sensitive contexts. However, traditional evaluation methods may fail to combine real-time measures, an "objective" approach and data contextualization. In this review we look at how adding neuroimaging techniques can respond to such needs. We focus on electroencephalography (EEG), as it could be handled effectively during a dedicated evaluation phase. We identify workload, attention, vigilance, fatigue, error recognition, emotions, engagement, flow and immersion as being recognizable by EEG. We find that workload, attention and emotions assessments would benefit the most from EEG. Moreover, we advocate to study further error recognition through neuroimaging to enhance usability and increase user experience.
Habermas e a educa??o: racionalidade comunicativa, diagnóstico crítico e emancipa??o
Mühl, Eldon Henrique;
Educa??o & Sociedade , 2011, DOI: 10.1590/S0101-73302011000400008
Abstract: this work tries to clarify the impacts of the theory of communicative rationality and habermas' theory of modernity on the educational field. afer developing the main ideas of both theories, it presents a brief diagnostic of the current crisis of rationality and education. its references are two concepts that are central to the author: the world of life and the system. the text then analyzes the implications of the communicative theory in the educational field and evaluates the efficiency of the german thinker's theses from a pedagogical point of view. it highlights both the pedagogical consequences of the philosophical change from the paradigm of the philosophy of the subject to that of intersubjectivity and the role philosophy played in this reconstruction in order to found a critical pedagogical proposal based on habermas' theory of communicative action.
IL-2 Stimulated but Not Unstimulated NK Cells Induce Selective Disappearance of Peripheral Blood Cells: Concomitant Results to a Phase I/II Study
Claudia Brehm, Sabine Huenecke, Andrea Quaiser, Ruth Esser, Melanie Bremm, Stephan Kloess, Jan Soerensen, Hermann Kreyenberg, Christian Seidl, Petra S. A. Becker, Heiko Mühl, Thomas Klingebiel, Peter Bader, Jakob R. Passweg, Dirk Schwabe, Ulrike Koehl
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0027351
Abstract: In an ongoing clinical phase I/II study, 16 pediatric patients suffering from high risk leukemia/tumors received highly purified donor natural killer (NK) cell immunotherapy (NK-DLI) at day (+3) +40 and +100 post haploidentical stem cell transplantation. However, literature about the influence of NK-DLI on recipient's immune system is scarce. Here we present concomitant results of a noninvasive in vivo monitoring approach of recipient's peripheral blood (PB) cells after transfer of either unstimulated (NK-DLI(unstim)) or IL-2 (1000 U/ml, 9–14 days) activated NK cells (NK-DLI(IL-2 stim)) along with their ex vivo secreted cytokine/chemokines. We performed phenotypical and functional characterizations of the NK-DLIs, detailed flow cytometric analyses of various PB cells and comprehensive cytokine/chemokine arrays before and after NK-DLI. Patients of both groups were comparable with regard to remission status, immune reconstitution, donor chimerism, KIR mismatching, stem cell and NK-DLI dose. Only after NK-DLI(IL-2 stim) was a rapid, almost complete loss of CD56(bright)CD16(dim/?) immune regulatory and CD56(dim)CD16(+) cytotoxic NK cells, monocytes, dendritic cells and eosinophils from PB circulation seen 10 min after infusion, while neutrophils significantly increased. The reduction of NK cells was due to both, a decrease in patients' own CD69(?) NCR(low)CD62L(+) NK cells as well as to a diminishing of the transferred cells from the NK-DLI(IL-2 stim) with the CD56(bright)CD16(+/?)CD69(+)NCR(high)CD62L(?) phenotype. All cell counts recovered within the next 24 h. Transfer of NK-DLI(IL-2 stim) translated into significantly increased levels of various cytokines/chemokines (i.e. IFN-γ, IL-6, MIP-1β) in patients' PB. Those remained stable for at least 1 h, presumably leading to endothelial activation, leukocyte adhesion and/or extravasation. In contrast, NK-DLI(unstim) did not cause any of the observed effects. In conclusion, we assume that the adoptive transfer of NK-DLI(IL-2 stim) under the influence of ex vivo and in vivo secreted cytokines/chemokines may promote NK cell trafficking and therefore might enhance efficacy of immunotherapy.
SWPS3 – fast multi-threaded vectorized Smith-Waterman for IBM Cell/B.E. and ×86/SSE2
Adam Szalkowski, Christian Ledergerber, Philipp Kr?henbühl, Christophe Dessimoz
BMC Research Notes , 2008, DOI: 10.1186/1756-0500-1-107
Abstract: Our benchmarking results show that swps3 is currently the fastest implementation of a vectorized Smith-Waterman on the Cell/BE, outperforming the only other known implementation by a factor of at least 4: on a Playstation 3, it achieves up to 8.0 billion cell-updates per second (GCUPS). Using the SSE2 instruction set, a quad-core Intel Pentium can reach 15.7 GCUPS. We also show that swps3 on this CPU is faster than a recent GPU implementation. Finally, we note that under some circumstances, alignments are computed at roughly the same speed as BLAST, a heuristic method.The Cell/BE can be a powerful platform to align biological sequences. Besides, the performance gap between exact and heuristic methods has almost disappeared, especially for long protein sequences.Alignments are used in bioinformatics to compare biological sequences. The gold standard of sequence alignment is the optimal local sequence alignment with affine gap costs by Smith and Waterman [1,2]. Modern implementations achieve high performances through the use of SIMD instructions, which perform operations on multiple values in parallel. Such vectorized implementations for general purpose desktop processors include previous work by Wozniak [3], Rognes and Seeberg [4], and Farrar [5]. The latter is by a significant margin the fastest implementation on ×86 architectures with SSE2 (streaming SIMD extensions) instruction set. As for other platforms, Sachdeva et al. [6] ported the Altivec kernel of ssearch34 from the FASTA package [7,3] to the Cell/BE, but no implementation is publicly available, according to our knowledge. Another solution has been provided by Manavski and Valle [8] on general purpose graphics hardware.In this article, we introduce swps3, an implementation of the Smith-Waterman algorithm that extends Farrar's work to the IBM Cell/BE platform. There, the improvement in runtime over results reported by Sachdeva et al. [6] are at least fourfold. The code also improves Farrar's work on ×86 arch
Our Economy  [PDF]
Christian Müller*
Modern Economy (ME) , 2011, DOI: 10.4236/me.2011.24063
Abstract: I discuss the strengths and weaknesses of the current predominant approach to macroeconomic modelling of asset prices and suggest an alternative perspective. This alternative rests on the insight that the economy is the result of individual decisions. The industry standard has it, however, that individual action is ruled by objective, general laws instead. Changing the point of view allows to reconcile numerous puzzles and paves the way for a promising new research agenda.
Is Faster Necessarily Better? 3G Take-Up Rates and the Implications for Next Generation Services  [PDF]
Christian M. Dippon
Int'l J. of Communications, Network and System Sciences (IJCNS) , 2012, DOI: 10.4236/ijcns.2012.58057
Abstract: Most countries have allocated 3G spectrum and are in the process of allocating advanced wireless services and 4G spectrum, both of which promise subscribers significantly faster broadband speeds. Operators anticipate that subscribers will adopt these services swiftly thereby justifying the large investments. Yet, why is it the case that only two countries in the world have more 3G than 2G subscribers? This study examines the drivers behind 3G diffusion using linear probability models and qualitative choice (logit) analysis. Time series diffusion models show that diffusion patterns follow an s-curve pattern, favoring the Gompertz curve.
An update on TroVax for the treatment of progressive castration-resistant prostate cancer
Abern M, Kaufman HL, Latchamsetty K
OncoTargets and Therapy , 2011, DOI: http://dx.doi.org/10.2147/OTT.S14271
Abstract: n update on TroVax for the treatment of progressive castration-resistant prostate cancer Review (3098) Total Article Views Authors: Abern M, Kaufman HL, Latchamsetty K Published Date May 2011 Volume 2011:4 Pages 33 - 41 DOI: http://dx.doi.org/10.2147/OTT.S14271 Michael Abern1, Howard L Kaufman2, Kalyan Latchamsetty1 1Department of Urology, Rush University Medical Center, Chicago, IL, USA; 2Department of General Surgery and Immunology and Microbiology, Rush University Medical Center, Chicago, IL, USA Abstract: Prostate cancer is a common human malignancy with few effective therapeutic options for treating advanced castration-resistant disease. The potential therapeutic effectiveness of immunotherapy and vaccines, in particular, has gained popularity based on the identification of prostate-associated antigens, potent expression vectors for vaccination, and data from recent clinical trials. A modified vaccinia Ankara (MVA) virus expressing 5T4, a tumor-associated glycoprotein, has shown promise in preclinical studies and clinical trials in patients with colorectal and renal cell carcinoma. This review will discuss the rationale for immunotherapy in prostate cancer and describe preclinical and limited clinical data in prostate cancer for the MVA-5T4 (TroVax ) vaccine.
Associations between functional ability and life satisfaction in the oldest old: results from the longitudinal population study Good Aging in Sk ne
Enkvist , Ekstr m H, Elmst hl S
Clinical Interventions in Aging , 2012, DOI: http://dx.doi.org/10.2147/CIA.S33610
Abstract: ssociations between functional ability and life satisfaction in the oldest old: results from the longitudinal population study Good Aging in Sk ne Original Research (2094) Total Article Views Authors: Enkvist , Ekstr m H, Elmst hl S Published Date August 2012 Volume 2012:7 Pages 313 - 320 DOI: http://dx.doi.org/10.2147/CIA.S33610 Received: 05 May 2012 Accepted: 01 June 2012 Published: 20 August 2012 sa Enkvist, Henrik Ekstr m, S lve Elmst hl Department of Health Sciences, Lund University, Malm University Hospital, Malm , Sweden Objectives: To describe change in functional ability in the oldest-old population during 3 years and examine its relation to life satisfaction (LS). A total of 681 individuals aged 78 and older from the population-based study Good Aging in Sk ne took part. Methods: Functional ability was assessed using Sonn and sberg's Activities of Daily Living (ADL) scale and related to LS assessed by Neugarten et al's Life Satisfaction Index A (LSI-A). Results: Fifty-one percent of 87–93-year-olds reported ADL decline during 3 years. Individuals reporting impaired ADL had a mean LSI-A value of 23.0 compared to 26.4 in those unchanged. ADL decline had a stronger negative effect on LS in the younger group (78–84 years), r = 0.207, P < 0.001. In a multiple regression model, one score's decline in ADL capacity corresponded to 1.5 scores lower LS (P < 0.001). Discussion: Effort put into keeping the oldest old on a high level of functional ability has the potential to maintain the LS of this population.
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