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Search Results: 1 - 10 of 36092 matches for " Chieh-Hsiang Lu "
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How the Impact of Median Neuropathy on Sensorimotor Control Capability of Hands for Diabetes: An Achievable Assessment from Functional Perspectives
Haw-Yen Chiu, Hsiu-Yun Hsu, Li-Chieh Kuo, Fong-Chin Su, Hui-I Yu, Shih-Che Hua, Chieh-Hsiang Lu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094452
Abstract: To comprehend the sensorimotor control ability in diabetic hands, this study investigated the sensation, motor function and precision pinch performances derived from a pinch-holding-up activity (PHUA) test of the hands of diabetic patients and healthy subjects. The precision, sensitivity and specificity of the PHUA test in the measurements of diabetic patients were also analyzed. We hypothesized that the diabetic hands would have impacts on the sensorimotor functions of the hand performances under functionally quantitative measurements. One hundred and fifty-nine patients with clinically defined diabetes mellitus (DM) and 95 age- and gender-matched healthy controls were included. Semmes-Weinstein monofilament (SWM), static and moving two-point discrimination (S2PD and M2PD), maximal pinch strength and precision pinch performance tests were conducted to evaluate the sensation, motor and sensorimotor status of the recruited hands. The results showed that there were significant differences (all p<0.05) in SWM, S2PD, M2PD and maximum pinch strength between the DM and control groups. A higher force ratio in the DM patients than in the controls (p<0.001) revealed a poor ability of pinch force adjustment in the DM patients. The percentage of maximal pinch strength was also significantly different (p<0.001) between the DM and control groups. The sensitivity, specificity and area under the receiver operating characteristic curve were 0.85, 0.51, and 0.724, respectively, for the PHUA test. Statistically significant degradations in sensory and motor functions and sensorimotor control ability were observed in the hands of the diabetic patients. The PHUA test could be feasibly used as a clinical tool to determine the sensorimotor function of the hands of diabetic patients from a functional perspective.
C. elegans EIF-3.K Promotes Programmed Cell Death through CED-3 Caspase
Chun-Yi Huang, Jia-Yun Chen, Shu-Chun Wu, Chieh-Hsiang Tan, Ruei-Ying Tzeng, Pei-Ju Lu, Yu-Feng Wu, Ruey-Hwa Chen, Yi-Chun Wu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036584
Abstract: Programmed cell death (apoptosis) is essential for the development and homeostasis of metazoans. The central step in the execution of programmed cell death is the activation of caspases. In C. elegans, the core cell death regulators EGL-1(a BH3 domain-containing protein), CED-9 (Bcl-2), and CED-4 (Apaf-1) act in an inhibitory cascade to activate the CED-3 caspase. Here we have identified an additional component eif-3.K (eukaryotic translation initiation factor 3 subunit k) that acts upstream of ced-3 to promote programmed cell death. The loss of eif-3.K reduced cell deaths in both somatic and germ cells, whereas the overexpression of eif-3.K resulted in a slight but significant increase in cell death. Using a cell-specific promoter, we show that eif-3.K promotes cell death in a cell-autonomous manner. In addition, the loss of eif-3.K significantly suppressed cell death-induced through the overexpression of ced-4, but not ced-3, indicating a distinct requirement for eif-3.K in apoptosis. Reciprocally, a loss of ced-3 suppressed cell death induced by the overexpression of eif-3.K. These results indicate that eif-3.K requires ced-3 to promote programmed cell death and that eif-3.K acts upstream of ced-3 to promote this process. The EIF-3.K protein is ubiquitously expressed in embryos and larvae and localizes to the cytoplasm. A structure-function analysis revealed that the 61 amino acid long WH domain of EIF-3.K, potentially involved in protein-DNA/RNA interactions, is both necessary and sufficient for the cell death-promoting activity of EIF-3.K. Because human eIF3k was able to partially substitute for C. elegans eif-3.K in the promotion of cell death, this WH domain-dependent EIF-3.K-mediated cell death process has potentially been conserved throughout evolution.
A Genome-Wide Association Study Identifies Susceptibility Variants for Type 2 Diabetes in Han Chinese
Fuu-Jen Tsai equal contributor,Chi-Fan Yang equal contributor,Ching-Chu Chen equal contributor,Lee-Ming Chuang,Chieh-Hsiang Lu,Chwen-Tzuei Chang,Tzu-Yuan Wang,Rong-Hsing Chen,Chiung-Fang Shiu,Yi-Min Liu,Chih-Chun Chang,Pei Chen,Chien-Hsiun Chen,Cathy S. J. Fann,Yuan-Tsong Chen ,Jer-Yuarn Wu
PLOS Genetics , 2010, DOI: 10.1371/journal.pgen.1000847
Abstract: To investigate the underlying mechanisms of T2D pathogenesis, we looked for diabetes susceptibility genes that increase the risk of type 2 diabetes (T2D) in a Han Chinese population. A two-stage genome-wide association (GWA) study was conducted, in which 995 patients and 894 controls were genotyped using the Illumina HumanHap550-Duo BeadChip for the first genome scan stage. This was further replicated in 1,803 patients and 1,473 controls in stage 2. We found two loci not previously associated with diabetes susceptibility in and around the genes protein tyrosine phosphatase receptor type D (PTPRD) (P = 8.54×10?10; odds ratio [OR] = 1.57; 95% confidence interval [CI] = 1.36–1.82), and serine racemase (SRR) (P = 3.06×10?9; OR = 1.28; 95% CI = 1.18–1.39). We also confirmed that variants in KCNQ1 were associated with T2D risk, with the strongest signal at rs2237895 (P = 9.65×10?10; OR = 1.29, 95% CI = 1.19–1.40). By identifying two novel genetic susceptibility loci in a Han Chinese population and confirming the involvement of KCNQ1, which was previously reported to be associated with T2D in Japanese and European descent populations, our results may lead to a better understanding of differences in the molecular pathogenesis of T2D among various populations.
The Effect of Mg Dopant and Oxygen Partial Pressure on Microstructure and Phase Transformation of Thin Films
Lay Gaik Teoh,Wei-Hau Lu,Ting Hsiang Lin,Ying-Chieh Lee
Journal of Nanomaterials , 2012, DOI: 10.1155/2012/539657
Abstract: Mg-doped zinc titanate (ZnTiO3) films were prepared using RF magnetron sputtering. Subsequent annealing of the as-deposited films was performed at a temperature ranging from 600 to 900°C for 2 hours with a heating rate of 5°C/min in air. It was found that the as-deposited films were amorphous and contained 2.77 at.% magnesium. This was further confirmed by the onset of crystallization that took place at annealing temperatures of 600°C. The results showed that single Zn2Ti3O8 phase was existed at 600°C. When annealing is at 700°C, the results revealed that mainly a hexagonal ZnTiO3 phase accompanying a Zn2Ti3O8 minor phase was observed. When annealing is at 900°C, the results showed that single hexagonal ZnTiO3 phase is stable at 900°C. It means that ZnTiO3 phase containing no Mg is unstable at 900°C and is decomposed from hexagonal ZnTiO3 to cubic Zn2TiO4 and rutile TiO2 at 900°C. In addition, the effect of oxygen partial pressure for the films deposited on the phase transformations and microstructures of zinc titanites thin film was investigated.
Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs
Chieh-Fang Cheng,I-Huang Lu,Hsiang-Wen Tseng,Chung-Yuan Sun
Evidence-Based Complementary and Alternative Medicine , 2013, DOI: 10.1155/2013/958025
Bayesian Fisher's Discriminant for Functional Data
Yao-Hsiang Yang,Lu-Hung Chen,Chieh-Chih Wang,Chu-Song Chen
Computer Science , 2014,
Abstract: We propose a Bayesian framework of Gaussian process in order to extend Fisher's discriminant to classify functional data such as spectra and images. The probability structure for our extended Fisher's discriminant is explicitly formulated, and we utilize the smoothness assumptions of functional data as prior probabilities. Existing methods which directly employ the smoothness assumption of functional data can be shown as special cases within this framework given corresponding priors while their estimates of the unknowns are one-step approximations to the proposed MAP estimates. Empirical results on various simulation studies and different real applications show that the proposed method significantly outperforms the other Fisher's discriminant methods for functional data.
Trypsin-induced proteome alteration during cell subculture in mammalian cells
Hsiang-Ling Huang, Hsiang-Wei Hsing, Tzu-Chia Lai, Yi-Wen Chen, Tian-Ren Lee, Hsin-Tsu Chan, Ping-Chiang Lyu, Chieh-Lin Wu, Ying-Chieh Lu, Szu-Ting Lin, Cheng-Wen Lin, Chih-Ho Lai, Hao-Teng Chang, Hsiu-Chuan Chou, Hong-Lin Chan
Journal of Biomedical Science , 2010, DOI: 10.1186/1423-0127-17-36
Abstract: In this study, a triplicate 2D-DIGE strategy has been performed to monitor trypsin-induced proteome alterations. The differentially expressed spots were identified by MALDI-TOF MS and validated by immunoblotting.36 proteins are found to be differentially expressed in cells treated with trypsin, and proteins that are known to regulate cell metabolism, growth regulation, mitochondrial electron transportation and cell adhesion are down-regulated and proteins that regulate cell apoptosis are up-regulated after trypsin treatment. Further study shows that bcl-2 is down-regulated, p53 and p21 are both up-regulated after trypsinization.In summary, this is the first report that uses the proteomic approach to thoroughly study trypsin-induced cell physiological changes and provides researchers in carrying out their experimental design.Plasma membrane proteins are responsible for a wide variety of functions essential to maintaining normal physiological activities. For example, when EGF receptor families, a group of proteins located in the plasma membrane that act as growth receptors, transmit external signals into the cell interior, cell's physiological activities are often altered in response to external signals. In addition, adhesive proteins, such as the cadherin families [1] in the cell membrane, provide anchors to link cytoskeleton proteins with extracellular matrix to regulate cell migration and cell adhesion. The dysregulations of membrane proteins cause numerous diseases such as during tumorigenesis, malignant transformation of epithelial cells frequently attends with loss of E-cadherin expression and induction of expression of mesenchymal membrane proteins like N-cadherin [2,3]. Moreover, mutations of ErbB-2 receptors lead to the occurrence of gastric cancer [4] and hepatocellular cancer [5].Two-dimensional gel electrophoresis (2-DE) has been widely used for profiling cellular proteins and some of the nonionic and zwitterionic detergents such as thiourea and CHAPS have
10 Gb/s Optical Interconnection on Flexible Optical Waveguide in Electronic Printed Circuit Board  [PDF]
Shih-Hsiang Hsu, Chih-Yuan Tsou, Chih-Ming Wang, Sheng-Chieh Tseng
Optics and Photonics Journal (OPJ) , 2013, DOI: 10.4236/opj.2013.32B059

In this paper, we proposed 10 Gb/s transmission using 4-channel polymer waveguides on the optical electronic printed circuit board. It was simulated by the ray tracing method for tolerance study of optical interconnection and fabrication. In order for easy fabrication and high position accuracy, the polymer waveguides were forming silver coated 45° reflective mirrors by dicing method and e-beam deposition for 90° light beam turning. The coupling loss was demonstrated in different polishing grit sizes. The optical interconnection in board-embed 4-channel flexible waveguides was demonstrated with a low propagation loss of 0.1 dB/cm and a clear eye diagram at 2.5 Gb/s data rate per channel.

The Role of Interferon-γ Inducible Protein-10 in a Mouse Model of Acute Liver Injury Post Induced Pluripotent Stem Cells Transplantation
Che-Chang Chan, Ling-Yi Cheng, Jean Lu, Yi-Hsiang Huang, Shih-Hwa Chiou, Ping-Hsing Tsai, Teh-Ia Huo, Han-Chieh Lin, Fa-Yauh Lee
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050577
Abstract: Background Liver injuries are important medical problems that require effective therapy. Stem cell or hepatocyte transplantation has the potential to restore function of the damaged liver and ameliorate injury. However, the regulatory factors crucial for the repair and regeneration after cell transplantation have not been fully characterized. Our study investigated the effects and the expression of the regulatory factors in mouse models of acute liver injury either transplanted with the induced pluripotent stem cells (iPS) or the hepatocytes that differentiated from iPS cells (iHL). Methods/Principal Findings Mice received CCl4 injection and were randomized to receive vehicle, iPS, or iHL transfusions vial tail veins and were observed for 24, 48 or 72 hours. The group of mice with iPS transplantation performed better than the group of mice receiving iHL in reducing the serum alanine aminotransferase, aspartate aminotransferase, and liver necrosis areas at 24 hours after CCl4 injury. Moreover, iPS significantly increased the numbers of proliferating hepatocytes at 48 hours. Cytokine array identified that chemokine IP-10 could be the potential regulatory factor that ameliorates liver injury. Further studies revealed that iPS secreted IP-10 in vitro and transfusion of iPS increased IP-10 protein and mRNA expressions in the injured livers in vivo. The primary hepatocytes and non-parenchyma cells were isolated from normal and injured livers. Hepatocytes from injured livers that received iPS treatment expressed more IP-10 mRNA than their non-hepatocyte counter-parts. In addition, animal studies revealed that administration of recombinant IP-10 (rIP-10) effectively reduced liver injuries while IP-10-neutralizing antibody attenuated the protective effects of iPS and decreased hepatocyte proliferation. Both iPS and rIP-10 significantly reduced the 72-hour mortality rate in mice that received multiple CCl4-injuries. Conclusions/Significance These findings suggested that IP-10 may have an important regulatory role in facilitating the repair and regeneration of injured liver after iPS transplantation.
The Influential Roles of Antibiotics Prophylaxis in Cirrhotic Patients with Peptic Ulcer Bleeding after Initial Endoscopic Treatments
Shih-Cheng Yang, Jen-Chieh Chen, Wei-Chen Tai, Cheng-Kun Wu, Chen-Hsiang Lee, Keng-Liang Wu, Yi-Chun Chiu, Jing-Houng Wang, Sheng-Nan Lu, Seng-Kee Chuah
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096394
Abstract: The influential roles of antibiotic prophylaxis on cirrhotic patients with peptic ulcer bleeding are still not well documented. The purpose of this study is to clarify these influential roles and to identify the risk factors associated with rebleeding, bacterial infection and in-hospital mortality. A cross-sectional, chart review study was conducted on 210 cirrhotic patients with acute peptic ulcer hemorrhage who underwent therapeutic endoscopic procedures. Patients were divided into group A (with prophylactic intravenous ceftriaxone, n = 74) and group B (without antibiotics, n = 136). The outcomes were length of hospital days, prevention of infection, rebleeding rate and in-hospital mortality. Our results showed that more patients suffered from rebleeding and infection in group B than group A (31.6% vs. 5.4%; p<0.001 and 25% vs. 10.8%; p = 0.014 respectively). The risk factors for rebleeding were active alcoholism, unit of blood transfusion, Rockall score, model for end-stage liver disease score and antibiotic prophylaxis. The risk factors for infection were active alcoholism, Child-Pugh C, Rockall score and antibiotic prophylaxis. Rockall score was the predictive factor for in-hospital mortality. In conclusions, antibiotic prophylaxis in cirrhotic patients after endoscopic interventions for acute peptic ulcer hemorrhage reduced infections and rebleeding rate but not in-hospital mortality. Rockall score was the predictive factor of in-hospital mortality.
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