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Search Results: 1 - 10 of 112856 matches for " Chi-Chen Lin "
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Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility
Hsieh Yao-Yuan,Chang Chi-Chen,Lin Chich-Sheng
International Journal of Biological Sciences , 2006,
Abstract: Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters. Patients and methods: Specimens were divided into two groups: group 1. normospermia (n=20); group 2. oligoasthenospermia (n=31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared. Results: MDA concentrations in both groups were significantly different (1.52 ± 0.75 vs. 2.25 ± 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 ± 0.11 vs. 0.47 ± 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p =0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility. Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.
The Hypouricemic Effect of Balanophora laxiflora Extracts and Derived Phytochemicals in Hyperuricemic Mice
Shang-Tse Ho,Yu-Tang Tung,Chi-Chang Huang,Chao-Lin Kuo,Chi-Chen Lin,Suh-Ching Yang,Jyh-Horng Wu
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/910152
Abstract: The objective of this study is to evaluate the lowering of uric acid using Balanophora laxiflora extracts and derived phytochemicals on potassium-oxonate-(PO-) induced hyperuricemia in mice. The results revealed that ethyl acetate (EtOAc) fraction of B. laxiflora extracts exhibited strong xanthine-oxidase-(XOD-) inhibitory activity. In addition, among the 10 subfractions (EA1–10) derived from EtOAc fraction, subfraction 8 (EA8) exhibited the best XOD-inhibitory activity. Four specific phytochemicals, 1-O-(E)-caffeoyl-β-D-glucopyranose (1), 1-O-(E)-p-coumaroyl-β-D-glucopyranose (2), 1,3-di-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-β-D-glucopyranose (3), and 1-O-(E)-caffeoyl-4,6-(S)-hexahydroxydiphenoyl-β-D-glucopyranose (4), were further isolated and identified from this subfraction. Compounds 3 and 4 exhibited the strongest XOD-inhibitory activity compared with other compounds, and both hydrolyzable tannins were determined to be noncompetitive inhibitors according to the Lineweaver-Burk plot. On the other hand, the in vivo hypouricemic effect in hyperuricemic mice was consistent with XOD-inhibitory activity, indicating that B. laxiflora extracts and derived phytochemicals could be potential candidates as new hypouricemic agents.
Leaf Extracts of Calocedrus formosana (Florin) Induce G2/M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells
Sheau-Yun Yuan,Chi-Chen Lin,Shih-Lan Hsu,Ya-Wen Cheng,Jyh-Horng Wu,Chen-Li Cheng,Chi-Rei Yang
Evidence-Based Complementary and Alternative Medicine , 2011, DOI: 10.1155/2011/380923
Abstract: Calocedrus formosana (Florin) bark acetone/ethylacetate extracts are known to exert an antitumor effect on some human cancer cell lines, but the mechanism is yet to be defined. The aim of this study was to determine the effects of Florin leaf methanol extracts on the growth and apoptosis of human bladder cancer cell lines. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that the growth of these bladder cancer cells was potently inhibited by the Florin leaf extracts. The cell cycle of these extract-treated cells (TCCSUP cells) was arrested at the G2/M phase as determined by flow cytometry. Western blot analysis revealed the increases of cyclin B1 and Cdc2 kinase levels, alone with the decrease of phosphorylated Cdc2 kinase, after treating these cells with the extracts. An immunofluorescence assessment of β-tubulin showed decreased levels of polymerized tubulin in treated cells. However, the proteolytic cleavage of poly ADP-ribose polymerase and the activation of caspase-3/-8/-9 were all increased upon treatments of extracts. The concurrent increase of Bax and decrease of Bcl-2 levels indicated that the extracts could induce apoptosis in these treated cells. Taken together, these results suggest that the Florin leaf extracts may be an effective antibladder cancer agent.
Increasing levels of circulating Th17 cells and interleukin-17 in rheumatoid arthritis patients with an inadequate response to anti-TNF-α therapy
Der-Yuan Chen, Yi-Ming Chen, Hsin-Hua Chen, Chia-Wei Hsieh, Chi-Chen Lin, Joung-Liang Lan
Arthritis Research & Therapy , 2011, DOI: 10.1186/ar3431
Abstract: The frequencies of circulating Th17 cells and serum levels of Th17-related cytokines were determined using flow cytometry analysis and ELISA, respectively, in 48 RA patients both before (baseline) and six months after anti-TNF-α therapy. Therapeutic response was evaluated using European League Against Rheumatism (EULAR) response criteria.Significantly higher baseline frequencies of circulating Th17 cells and serum levels of interleukin (IL)-6, IL-17, IL-21, IL-23 and TNF-α were observed in active RA patients than in 12 healthy controls (all P < 0.001). After anti-TNF-α therapy, 36 patients (75%) were EULAR responders (20 good responders and 16 moderate responders) and 12 (25.0%) were non-responders. The mean levels of circulating Th17 cells and IL-17 significantly decreased (1.13% vs. 0.79%; 43.1 pg/ml vs. 27.8 pg/ml; respectively, both P < 0.001) in parallel with clinical remission in responders. Levels of IL-6, IL-21, IL-23 and TNF-α were significantly decreased after anti-TNF-α therapy in responders. In contrast, the mean levels of circulating Th17 cells and IL-17 significantly increased after anti-TNF-α therapy (2.94% vs. 4.23%; 92.1 pg/ml vs. 148.6 pg/ml; respectively, both P < 0.05) in non-responders. Logistic regression analysis identified a high baseline level of IL-17 as a significant predictor of poor therapeutic response.The beneficial effect of anti-TNF-α therapy might involve a decrease in Th17-related cytokines in responders, whereas rising levels of circulating Th17-cells and IL-17 were observed in patients with an inadequate response to anti-TNF-α therapy.Rheumatoid arthritis (RA) is characterized by the infiltration of macrophages and T cells into the joints, synovial hyperplasia, cartilage degradation and bone erosions [1]. Tumor necrosis factor (TNF)-α is a crucial inflammatory mediator in rheumatoid synovitis and subsequent tissue damage in RA [2,3]. Although TNF-α inhibitors can be an effective and well-tolerated therapy for RA patients [4-6], a
SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase
Robert Lee, Chi-Chen Fan, Yuh-Ming Hwu, Chung-Hao Lu, Ming-Huei Lin, Ying-Jie Chen, Sheng-Hsiang Li
Reproductive Biology and Endocrinology , 2011, DOI: 10.1186/1477-7827-9-38
Abstract: Seven patients who underwent a hysterectomy and samples of 120 archived patients' endometrial curettage or parts of the uterus that were formalin-fixed and embedded in paraffin. Western blotting was performed to evaluate the specificity and sensitivity of the antibody. Immunohistochemistry was conducted to localize the SERPINE2 expression site. Quantitative analysis was conducted to evaluate expression levels of SERPINE2 in various sub-phases of the menstrual cycle.The SERPINE2 protein was primarily detected in the uterine fluid during the mid- and late-secretory phases of the menstrual cycle. It was predominantly expressed in the luminal and glandular epithelium, less in the myometrium, and only dispersedly in certain stromal cells throughout the menstrual cycle. A quantitative analysis of expression levels of SERPINE2 in the glandular epithelium revealed that it was highly expressed in the endometrium during the secretory phase compared to the proliferative phase.The SERPINE2 protein is highly expressed in the endometrium during the secretory phase, indicating that it may participate in tissue remodeling involved in implantation.The plasminogen activator (PA) system refers to the PA and its cognate inhibitors [1]. The system includes two forms of PA, tissue-type (PLAT or tPA) and urokinase-type (PLAU or uPA), and four forms of serine protease inhibitors (SERPIN), including SERPINA5 (also called protein C inhibitor, PCI), SERPINB2 (also called plasminogen activator inhibitor 2, PAI-2), SERPINE1 (also called plasminogen activator inhibitor 1, PAI-1), and SERPINE2 (also called protease nexin-1, PN-1) [2].The PA system is associated with many reproductive processes, including ovulation, embryogenesis, and embryo implantation in female reproductive tissues [1,3]. How SERPIN modulates the proteolytic activities of PLAT/PLAU in reproductive tissue remodeling is of great importance.Tissue remodeling requires a fine-tuned balance between levels of proteases and their cogna
The cuttable C-related genotype and allele for the E-cadherin 3?-UTR Pml I polymorphism are associated with higher susceptibility to endometriosis
Hsieh, Yao-Yuan;Chang, Chi-Chen;Tsai, Fuu-Jen;Hsu, Chin-Moo;Lin, Cheng-Chieh;Tsai, Chang-Hai;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000500003
Abstract: epithelial cadherin (e-cadherin; cdh1) may influence pericellular proteolysis and intracellular signal transduction, which plays an essential part of tumor invasion. in our study we investigated the correlation between cdh1 gene polymorphism and endometriosis in two groups of pre-menopausal taiwanese women, group 1 (n = 150) consisting of women with severe stage iv endometriosis and group 2 (n = 159) of women with no endometriosis. the polymerase chain reaction (pcr) was used to identify the cuttable (c) and uncuttable (t) polymorphism of the cdh1-pml i gene (rs1801026) located on the 3?-untranslated region (3?-utr) of chromosome 16 and compare the genotypes and allelic frequencies of this gene in both groups. we found that the genotype and allele distributions of the cdh1-pml i c/t polymorphism were significantly different in both groups. in group 1 the cdh1*c frequency was 47.7% and the t frequency 52.3%, while the cc homozygote frequency was 6.7%, the tt homozygote 11.3% and the ct heterozygote 82%. in group 2 the cdh1*c frequency was 17% and the t frequency 83%, while the cc frequency was 0.6%, the tt 66.1% and the ct 33.3%. these data indicate that the cdh1 gene polymorphism may be associated with the development of severe endometriosis and that the cdh1 gene c allele is related to higher susceptibility to endometriosis.
Insulin-like growth factor II gene Apa I polymorphism is not associated with endometriosis susceptibility
Hsieh, Yao-Yuan;Chang, Chi-Chen;Tsai, Fuu-Jen;Peng, Ching-Tien;Yeh, Lian-Shun;Lin, Cheng-Chieh;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000200006
Abstract: insulin-like growth factor ii (igf2) has been shown to play a role in abnormal cell growth and carcinogenesis. we investigated if the igf2 gene apa i polymorphism at exon 9 was associated with the susceptibility to endometriosis, analyzing 120 women with moderate/severe endometriosis and 103 controls. the genotype frequencies did not differ statistically between the endometriosis (aa = 25.4, ab = 57.4, bb = 17.2%) and control (aa = 20.8 ab = 52.8, bb = 26.4%) groups. the allele frequencies did not differ either: a = 54.1, b = 45.9% among women with endometriosis and a = 47.2, b = 52.8% in the control group. therefore, no indication was found for an association of this polymorphism with endometriosis susceptibility.
Cytochrome P450c17alpha (CYP17) gene polymorphism is not associated with leiomyoma susceptibility
Yao-Yuan, Hsieh;Fuu-Jen, Tsai;Chi-Chen, Chang;Chang-Hai, Tsai;Cheng-Chieh, Lin;Lian-Shun, Yeh;
Genetics and Molecular Biology , 2002, DOI: 10.1590/S1415-47572002000400002
Abstract: estrogen plays a role in the pathogenesis of leiomyoma. the cyp17 gene codes for the cytochrome p450c17a enzyme, which is involved in the biosynthesis of estrogen. our aim was to investigate if cyp17 polymorphism could be a useful marker to predict the susceptibility to leiomyoma. our sample of female subjects was divided into two groups: (1) with leiomyoma (n = 159); (2) without leiomyoma (n = 128). a 169-bp fragment encompassing the a1/a2 polymorphic site of the cyp17 gene was amplified by polymerase chain reaction (pcr), restricted by enzyme mspa1i and electrophored on agarose gel. genotypes and allelic frequencies for this polymorphism in both groups were compared. there was no significant difference between the two groups regarding the distribution of the cyp17 gene polymorphism frequencies. the a1 homozygote/heterozygote/a2 homozygote proportions for cyp17 in both groups were: (1) 17.0/46.5/36.5%, and (2) 17.2/45.3/37.5%. the proportions for alleles a1 and a2 were also comparable in the two groups. a1 and a2 allele frequencies were: 7% (40.3/59) in group 1, and 2% (39.8/60) in group 2. no significant association was observed between the risk of leiomyoma and polymorphisms of the cyp 17 gene. so, cyp17 gene polymorphism does not appear to be a useful marker for the prediction of leiomyoma susceptibility.
Effects of Diosgenin on Myometrial Matrix Metalloproteinase-2 and -9 Activity and Expression in Ovariectomized Rats
Chi-Chen Chang, Tang-Ching Kuan, Yao-Yuan Hsieh, Ying-Jui Ho, Yu-Ling Sun, Chih-Sheng Lin
International Journal of Biological Sciences , 2011,
Abstract: Diosgenin, a traditional Yam extraction, has been used in hormone replacement for menopausal women. We aimed to investigate the influences of diosgenin administration upon the MMP-2 and -9 activity and expression and reproductive hormones of ovariectomized (OVX) rats, a model of menopausal status. Seven-week old female Wistar rats with bilateral OVX or sham operation (controls) were divided and administered different dosages of diosgenin (0, 10, 50, or 100 mg/kg/day) for 8 weeks. Serum was then sampled for progesterone (P4) and estradiol (E2) assay and uterine horns harvested. Myometrial MMP-2 and -9 activity and expression were surveyed and myometrial collagen expression was also assayed. The results show higher body weight in OVX rats across the 8 weeks post surgery and no significant differences were noted among OVX or Sham rats with diosgenin supplements. There were lower P4 and E2 concentrations in OVX rats compared to Sham rats, and higher P4 concentration of Sham rats post diosgenin supplement. MMP-2 and -9 mRNA expression and activity was lower in OVX rats, although higher MMP-2 and lower MMP-9 activity/mRNA expression was observed in OVX rats post diosgenin supplementation. Collagen mRNA expression was higher in OVX rats compared to Sham controls, and diosgenin administration decreased collagen mRNA expression in OVX rats. In conclusion, diosgenin is associated with gelatinase expression and collagen metabolism in OVX rats. Diosgenin administration can partially reverse the effects of OVX upon MMP functions and hormone status. Adequate diosgenin supplement might modulate myometrial gelatinase expression and collagen metabolism in menopausal subjects.
Disruption of Murine mp29/Syf2/Ntc31 Gene Results in Embryonic Lethality with Aberrant Checkpoint Response
Chia-Hsin Chen, Po-Chen Chu, Liekyeow Lee, Huang-Wei Lien, Tse-Ling Lin, Chi-Chen Fan, Peter Chi, Chang-Jen Huang, Mau-Sun Chang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033538
Abstract: Human p29 is a putative component of spliceosomes, but its role in pre-mRNA is elusive. By siRNA knockdown and stable overexpression, we demonstrated that human p29 is involved in DNA damage response and Fanconi anemia pathway in cultured cells. In this study, we generated p29 knockout mice (mp29GT/GT) using the mp29 gene trap embryonic stem cells to study the role of mp29 in DNA damage response in vivo. Interruption of mp29 at both alleles resulted in embryonic lethality. Embryonic abnormality occurred as early as E6.5 in mp29GT/GT mice accompanied with decreased mRNA levels of α-tubulin and Chk1. The reduction of α-tubulin and Chk1 mRNAs is likely due to an impaired post-transcriptional event. An aberrant G2/M checkpoint was found in mp29 gene trap embryos when exposed to aphidicolin and UV light. This embryonic lethality was rescued by crossing with mp29 transgenic mice. Additionally, the knockdown of zfp29 in zebrafish resulted in embryonic death at 72 hours of development postfertilization (hpf). A lower level of acetylated α-tubulin was also observed in zfp29 morphants. Together, these results illustrate an indispensable role of mp29 in DNA checkpoint response during embryonic development.
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