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Search Results: 1 - 10 of 10217 matches for " Charles Euloge Lamien "
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Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso
Wamtinga Richard Sawadogo,Aline Meda,Charles Euloge Lamien,Martin Kiendrebeogo
Journal of Biological Sciences , 2006,
Abstract: In this study the total phenolic and flavonoid content as well as the antioxidant activity of six Acanthaceae namely Blepharis lineariifolia PERS, Dicliptera verticillata (FORRSK.) C. CHRISTENS, Dyschoriste perrottetii (NEES) O. KTZE, Hygrophila auriculata (SCHUMACH.) HEINE, Lepidagathis anobrya NEES, Nelsonia canescens (LAM) SPRENG were evaluated. The total phenolic and flavonoid of their aqueous acetone extract were assessed by Folin-ciocalteu and AlCl3 method, respectively, whereas the antioxidant activities were determined by the DPPH method. Lepidagathis anobrya, Hygrophila auriculata and Nelsonia canescens which had the highest phenolic content, were found to possess the best antioxidant activities. The results suggest that these plants are good sources of antioxidants and support their use in cardiovascular and antiinflammatory diseases.
Development of a Cost-Effective Method for Capripoxvirus Genotyping Using Snapback Primer and dsDNA Intercalating Dye
Esayas Gelaye, Charles Euloge Lamien, Roland Silber, Eeva S. M. Tuppurainen, Reingard Grabherr, Adama Diallo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075971
Abstract: Sheep pox virus (SPPV), goat pox virus (GTPV) and lumpy skin disease virus (LSDV) are very closely related viruses of the Capripoxvirus (CaPV) genus of the Poxviridae family. They are responsible for sheep pox, goat pox and lumpy skin disease which affect sheep, goat and cattle, respectively. The epidemiology of capripox diseases is complex, as some CaPVs are not strictly host-specific. Additionally, the three forms of the disease co-exist in many sub-Saharan countries which complicates the identification of the virus responsible for an outbreak. Genotyping of CaPVs using a low-cost, rapid, highly specific, and easy to perform method allows a swift and accurate identification of the causative agent and significantly assists in selecting appropriate control and eradication measures, such as the most suitable vaccine against the virus during the outbreaks. The objective of this paper is to describe the design and analytical performances of a new molecular assay for CaPV genotyping using unlabelled snapback primers in the presence of dsDNA intercalating EvaGreen dye. This assay was able to simultaneously detect and genotype CaPVs in 63 samples with a sensitivity and specificity of 100%. The genotyping was achieved by observing the melting temperature of snapback stems of the hairpins and those of the full-length amplicons, respectively. Fourteen CaPVs were genotyped as SPPVs, 25 as GTPVs and 24 as LSDVs. The method is highly pathogen specific and cross platform compatible. It is also cost effective as it does not use fluorescently labelled probes, nor require high-resolution melting curve analysis software. Thus it can be easily performed in diagnostic and research laboratories with limited resources. This genotyping method will contribute significantly to the early detection and genotyping of CaPV infection and to epidemiological studies.
Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso
Aline Lamien-Meda,Charles Euloge Lamien,Moussa M.Y. Compaoré,Roland N.T. Meda,Martin Kiendrebeogo,Boukare Zeba,Jeanne F. Millogo,Odile G. Nacoulma
Molecules , 2008, DOI: 10.3390/molecules13030581
Abstract: A total of fourteen (14) species of wild edible fruits from Burkina Faso wereanalyzed for their phenolic and flavonoid contents, and their antioxidant activities usingthe DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic andtotal flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana andLannea microcarpa. Significant amounts of total phenolics were also detected in the otherfruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit alsoshowed the highest antioxidant activity using the three antioxidant assays. Fruits with highantioxidant activities were also found to possess high phenolic and flavonoid contents.There was a strong correlation between total phenolic and flavonoid levels and antioxidantactivities.
Antiacetylcholinesterase and antioxidant activity of essential oils from six medicinal plants from Burkina Faso
Kiendrebeogo, Martin;Coulibaly, Ahmed Y.;Nebie, Roger C. H.;Zeba, Boukaré;Lamien, Charles E.;Lamien-Meda, Aline;Nacoulma, Odile G.;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000008
Abstract: in this investigation, we evaluated essential oils from six medicinal plants from burkina faso for their antiacetylcholinesterase and antioxidant abilities. the chemotype of most active were also determined. the best antiacetylcholinesterase activities were recorded for the essential oils of eucalyptus camaldulensis (ic50 18.98 μ g/ml) and ocimum canum (ic50 36.16 μ g/ml). their chemotype have been related to the 1,8-cineole one. both essential oils demonstrated a linear mixed non competitive inhibition. the essential oil of ocimum basilicum which belong to the linalool-eugenol chemotype exhibited the best radical scavenging activity (ic50 3.82 μ g/ml) and reducing power (531.75 mg aae/g). in comparison with gallic and ascorbic acids, o. basilicum essential oil evidenced interesting antioxidant activities. the antiacetylcholinesterase and antioxidant activities of essential oils were discussed in regard with their chemical composition.
Cardiometabolic Phenotype and Arterial Stiffness in HIV-Positive Black African Patients  [PDF]
Daniel Lemogoum, William Ngatchou, Philippe Van De Borne, Valérie Ndobo, Marc Leeman, Euloge Yagnigni, Emiline Tiogou, Elisabeth Nga, Charles Kouanfack, Luc Van Bortel, Jean-Paul Degaute, Michel P. Hermans
Open Journal of Preventive Medicine (OJPM) , 2014, DOI: 10.4236/ojpm.2014.44024

Objective: To test the hypothesis that Human Immunodeficiency Virus (HIV) infection in Black patients is associated with increased cardiometabolic risk factors that may increase aortic stiffness assessed by pulse wave velocity (PWV). Methods: We matched 96 Cameroonian Controls to 238 (Un) treated HIV-positive patients [HIV] for age. In each participant, aortic PWV (ComplioR), blood pressures (BP), lipid profile and fasting blood glucose (FPG) were measured. Results: Waist circumference was lower in HIV than in Controls (both p < 0.001). The prevalences of impaired FPG (100-125 mg·dl-1), and of diabetes (FPG > 125 mg·dl-1

Composition and Antimicrobial Activities of Lippia multiflora Moldenke, Mentha x piperita L. and Ocimum basilicum L. Essential Oils and Their Major Monoterpene Alcohols Alone and in Combination
Ima?l Henri Nestor Bassolé,Aline Lamien-Meda,Balé Bayala,Souleymane Tirogo,Chlodwig Franz,Johannes Novak,Roger Charles Nebié,Mamoudou Hama Dicko
Molecules , 2010, DOI: 10.3390/molecules15117825
Abstract: Essential oils from leaves of Lippia multiflora, Mentha x piperita and Ocimum basilicum from Burkina Faso were analysed by GC–FID and GC–MS. Major components were p-cymene, thymol, b-caryophyllene, carvacrol and carvone for L. multiflora, menthol and iso-menthone for M. x piperita and, linalool and eugenol for O. basilicum. The essential oils and their major monoterpene alcohols were tested against nine bacterial strains using the disc diffusion and broth microdilution methods. The essential oils with high phenolic contents were the most effective antimicrobials. The checkerboard method was used to quantify the efficacy of paired combinations of essential oils and their major components. The best synergetic effects among essential oils and major components were obtained with combinations involving O. basilicum essential oil and eugenol, respectively. As phenolic components are characterized by a strong spicy aroma, this study suggests that the selection of certain combinations of EOs could help to reduce the amount of essential oils and consequently reduce any adverse sensory impact in food.
Football Practice and Behaviour of Learners to Primary School: Assimilation and Accommodation  [PDF]
Paulin Mandoumou, Aristide Ewamela, Euloge Moboza Ndongo, Joachim Bongbélé
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.714198
Abstract: In order to know whether the teaching of football played dispensed using the form obeyed the fundamental and permanent requirements thereof and located the learner in his motor behavior in the various phases of the sport soccer game in the Piagetian approach assimilation and accommodation, 59 students of CE2 (10 years), CM1 and CM2 (11 years) were observed and subjected to learning football. The results showed that age does not influence notoriously motor behavior in the implementation of technical and tactical actions football. However, it allowed observing clear differences in students’ abilities to perform certain technical and tactical actions in different sequences or phases of play. These capabilities have proven in defense and midfield for students and CE2 in attack for those of CM1 and CM2. Thus, assimilation has been the mode of adaptation of elementary students in second year during attack, as well as that of CM1 and CM2 in defense and midfield. The accommodation was against by the observable behavioral repository for learners of CM in attack and defense in those CE2.
On the Patterns of the Occurrence of Diarrheas in the Southern Benin  [PDF]
Hervé Thierry Azonhè, R. Glèlè Kaka?, Atidegla Capo Séraphin, Kossi Euloge Agbossou
Journal of Environmental Protection (JEP) , 2013, DOI: 10.4236/jep.2013.44042

The present study focused on the analysis of the risk factors of diarrhea in Southern Benin. Data have been collected from 442 peasant households on the basis of a stratified random sampling method in the targeted communities, with information on the source of water provision, the location of the village, the usual defecation place, the site of refusing dumping and the presence of trash dump in the vicinity of the household. Data have been analyzed using descriptive statistics. In addition, analyses of variance and log-linear analyses have been applied to test the various factors in the outbreak of diarrheas. Results have allowed a hierarchical organization of the drinking water sources with regards to their use and to the cases of diarrheas (boreholes: p < 0.001; rainwater and territorial water: p < 0.001; wells: p = 0.547 and pipe-borne water: p = 0.277. If we rule out people’s insanitary behaviors, the impact of some risk factors such as the sites of waste disposal (2%) and excreta disposal (1%) is of little influence in comparison to the water supply source and the place of residence, which play respectively for 62% and 30% in the outbreak of episodes of diarrheas in households. It follows that, to curb the diarrhea morbidity, there is the need to improve the water supply policy and the environmental sanitation.

Evaluation of the Microbiological and Nutritional Quality of Fermented-Dried Lesser African Threadfin (Galeoides decadactylus) Used as Food Supplement in Southern Benin  [PDF]
René G. Dègnon, Euloge S. Adjou, Edwige Dahouenon-Ahoussi, Mohamed M. Soumanou, Emile Fiogbé
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.61005

Lesser African threadfin (Galeoides decadactylus) is a nutritious marine fish, popular and widely used for drying-fermentation. The present work aims to evaluate the nutritional and microbiological quality of dried-fermented Lesser African Threadfin, currently used in food preparing in southern Benin. Four major sites of drying-fermentation fishes in southern Benin were investigated and dried-fermented Lesser African Threadfin was collected for quality control. Results indicated that the dried-fermented fishes were good sources of nutriments with a moisture content ranging from 44.62% ± 0.68% to 55.33% ± 0.23%. Proteins contents are ranged from 15.26% ± 0.32% to 22.95% ± 0.71%. All samples analyzed were rich in minerals such as magnesium, phosphorus, iron, with a

Influence of Self-Esteem in the Withdrawal EPS Students from Catholic Schools of Humanities in Kinshasa  [PDF]
Aristide Ewamela, Paulin Mandoumou, Agnes Kena Lubika, Euloge Moboza Ndongo, Joachim Bongbele
Advances in Physical Education (APE) , 2016, DOI: 10.4236/ape.2016.63025
Abstract: In order to examine the influence of self-esteem on the disengagement of students in EPS, 305 girls and 456 boys from Catholic schools of humanities (experimental group), 116 girls and 133 boys from consular schools (control group) have answered the questionnaire from Ninot et al. (2000). The averages of the two groups were compared using student test. The results showed that subjects in the experimental group showed low values of global self-esteem, the physical value, strength, endurance and athletic competence. These results indicate a parallel disengagement significantly remarkable EPS for girls and boys in the experimental group compared to their counterparts in the control group. These results suggest that the low value of self-esteem is the disengagement factor in EPS within Catholic schools of humanities in Kinshasa.
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