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Level of Lead Contamination on Electrical Equipments Electronic Devices Repairmen and Working Environment in the Area of Nakhonratchasima Province, Thailand
Weerapol Wongpraphan,Adisak Singseewo,Tawatchai Thanee,Chantana Padungtod
Research Journal of Medical Sciences , 2012, DOI: 10.3923/rjmsci.2010.88.93
Abstract: A study of the electrical equipment and electronics repaired shops, where in the areas of local government of Khamsakaesang, Nonthai, Nonsoong and Khong SubDistrict, Nakhonratchasima Province, which were in the total amount of 24 repairshops 39 repairmen. Data collection has been done by doing questionnaire and inspecting the levels of blood lead contamination and environmental lead contamination with Graphite Furnace Atomic Absorption Spectrophotometer (GFAAS) found that the average level of blood lead was at 8.58 μg dL-1, which the lowest blood lead level was at 1.26 μg dL-1 and the highest blood lead level was at 25.54 μg dL-1. The repairmen, who have different education and work experience levels were statistically having different blood lead level (p<0.05), apart from that there was a found of dust with lead contamination in working areas in the average level of 173.26 g kg-1, which the lowest level was at 49.6 g kg-1 and the highest level was at 354 g kg-1, which is much higher than the specified standard of US EPA. Apparently, repairmen are in risk of health threaten from their unsafe work condition, do not use personal protective equipments, while welding lead their working environment have highly dust lead contamination and terminating hazardous in incorrect way, which cause an effect for their family member, especially childhood and environment could be contaminated directly and indirectly. Then, the related government sectors should be actively operating, solving and managing occupational health and sanitation services for this career.
The Effect of Group Participatory Learning Process on Knowledge, Attitude and Practice for Lead Contamination Prevention on Electrical Equipments and Electronic Devices Repairmen and Work Environment in the Area of Nakhonratchasima Province, Thailand
Weerapol Wongpraphan,Adisak Singseewo,Tawatchai Thanee,Chantana Padungtod
The Social Sciences , 2013, DOI: 10.3923/sscience.2010.202.207
Abstract: This research has a purpose to educate the effect of group participatory learning process on knowledge, attitude and practice for lead contamination prevention on electrical equipments and electronic devices repairmen and work environment in the area of Nakhonratchasima Province, Thailand. There were 39 volunteers as the example group in this research by the researcher producing a training plan and questionnaire relating to general information, knowledge, attitude and practice also finding a qualitative instrument in order to gather information before the training such as interview and soil powder collection at work places and sent them to laboratory to be investigated and analyzed. Furthermore, the training operation was done by the group of participatory learning process with operative occupational health with 3 steps as following; step1: Health Education, step 2: Job Safety Analysis and step 3: Evaluation of Participation. Four months operation were used in repeatedly gathering information after finished the process of informative analysis by Percentage (%), Mean (), Standard Deviation (SD) and Statistic Comparison which used t-test and F-test (One-way ANOVA). The result found that after had training programme, most of repairmen have more general and each aspects of knowledge, attitude and practice on lead contamination prevention than before taking the programme statistically significant rate of (p<0.05) and importantly, the contaminated lead level in soil powder after the training programme was less than before taking the programme statistically significant rate of (p<0.05). Effectively, the process of group participatory learning was applied in operative process of sanitary profession which making more knowledge, attitude and practice in repairmen s lead contamination prevention as well as the related government sectors should considerably put this form of training into workers health improvement action relating to lead contamination safety and must be friendly to the environment.
Essential oil of the flowers of Azadirachta indica (Meliaceae)
Chantana Aromdee,Nongluksna Sriubolmas
Songklanakarin Journal of Science and Technology , 2006,
Abstract: Essential oil of the flowers of Azadirachta indica was obtained at 0.001% yield by steam distillation. It mainly consisted of α-cubebene (3.04%), copaene (7.03 %), humulene (3.7 %), δ-cadinene (9.43 %) and a number of sesquiterpenes. The oil also contained a series of unidentified organosulphur compounds. These organosulphur compounds were picked up by the S34 isotope. The oil exhibited antimicrobial activity against Bacillus subtilis (ATCC 6633), Candida albicans (ATCC 10231) and Microsporum gypseum (Clinical isolated).
Quantitative analysis of some volatile components in Mimusops elengi L.
Chantana Aromdee,Butsayamat Rattanadon
Songklanakarin Journal of Science and Technology , 2009,
Abstract: Dried pikul flower (Mimusops elengi L., Sapotaceae) is used in many recipe s of Thai traditional medicine i.e. cardiotonic and stomachic. In this study, fresh and dried pikul flowers were investigated. The odour of pikul flower, even when it was dried, is very strong and characteristic. The constituents of volatile oils in fresh and dried pikul flowers extracted by ether were analysed by gas chromatography-mass spectrometry. 2-Phenylethanol, 4-hydroxybenzenemethanol and cinnamyl alcohol were mainly found in fresh flower, 10.49, 8.69 and 6.17%, respectively. Whereas those mainly found in dried flowers were long chain carboxylic acid ester and (Z)-9-octadecenoic acid, 5.37 and 4.71% of ether extract, respectively.An analytical method simultaneously determining benzyl alcohol, 2-phenylethanol and methyl paraben was developed by using the GC-FID method. The percent recoveries were 91.66, 104.59 and 105.28%, respectively. The intraday variations(% RSD) were 7.22, 6.67 and 1.86%; and the interday variation were 3.12, 2.52 and 3.55%, respectively. Detection limits were 0.005, 0.014 and 0.001 ppm, and quantitation limits were 0.015, 0.048 and 0.003 ppm, respectively. Benzyl alcohol, 2-phenylethanol and methyl paraben content of dried flowers (9 samples from various drug stores in Thailand and one sample from China) were 6.40-13.46, 17.57-196.57 and 27.35-355.53 ppm, respectively.
Synthesis and Evaluation of Anticonvulsant Activity of 6,8-Dimethoxy-3-methyl-1,2,3,4-tetrahydroisoquinoline in PTZ-Induced Seizure Model in Mice  [PDF]
Yothawathorn Pariyawongsakul, Chamnan Patarapanich, Chantana Boonyarat, Ploenthip Puthongking
Open Journal of Medicinal Chemistry (OJMC) , 2012, DOI: 10.4236/ojmc.2012.23009
Abstract: This study we describe the synthesis of a novel structure of anticonvulsant agent as 6,8-dimethoxy-3-methyl-1,2,3,4- tetrahydroisoquinoline by using GYKI52466, which was the potent anticonvulsant agent, as the lead molecule. Com-pound IV was synthesized and anticonvulsant effects was evaluated against Pentylenetetrazole (PTZ)-induced seizure model in mice. The acute anticonvulsant effect was tested with a single dose of 25 and 75 µmol/kg of the synthesis compound. Sodium valproate and normal saline were used as the reference standard and control, respectively. All compounds were injected intraperitoneally to each mouse an hour prior to seizure induced by injection of 60 mg/kg PTZ and observed their behavior for 30 minutes. The result showed that the IV at 75 µmol/kg could delay the latency to first twitch and decrease percent mortality compared to control group.
Spectrophotometric determination of total lactones in Andrographis paniculata Nees
Chantana Aromdee,Penkae Wichitchote,Napaporn Jantakun
Songklanakarin Journal of Science and Technology , 2005,
Abstract: A spectrophotometric method for determination of total lactones in Andrographis paniculata was established by using dinitrobenzoic acid and potassium hydroxide solutions as colour forming agents. The absorbance of the solution was determined at 536 nm. The linearity range was 12-72 × 10-6 g.ml-1. The detection limit was 1.2 μg, the quantitation limit was 4.23 μg. The intraday variation had an average of slope 6082.97 g.ml-1, % RSD 0.10; an average intercept 0.2786, %RSD 3.66 (n=3). The interday variation had an average of 6146 g.ml-1 with the %RSD of 6.30 and an average intercept 0.2628, %RSD 4.95 (n=4). The coefficients of determination were 0.998-0.999. The total lactones content, calculated as andrographolide, determined by this method was 8.61±0.52% (n=4) and by the official method, Thai Herbal Pharmacopoeia, was 8.12±0.34% (n=2). The results of the two methods do not differ significantly at P=0.05 (P(|t|>0.903 = 0.53)
Antioxidant activity and DNA fingerprint of four varieties of lotus stamens (Nelumbo nucifera Gaertn.)
Nithida Phonkot,Preeya Wangsomnuk,Chantana Aromdee
Songklanakarin Journal of Science and Technology , 2008,
Abstract: DNA fingerprints of stamens of four varieties of Nelumbo nucifera (Gaertn) were established and their antioxidant activity was studied. PCR amplification was used for identification of Lotus DNA by using OPS3, OPS11, OPS13 and OPE3 random decamer-primers. The result showed variety-specific markers of Pathum, Sattabongkot, Boontharik and Sattabuut varieties. The antioxidant activities of solutions in methanol and mixed-solvent of the four varieties were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) model. The antioxidant activities (IC50) of methanol solution of the four varieties were 68.306.30, 62.224.00, 31.603.40 and 40.901.50 g.mL-1, respectively, whereas those of the mixed-solvent solution were 2.210.06, 2.230.05, 1.290.02 and 1.830.07 mg.mL-1. The result showed that IC50 of both solutions of Sattabongkot were significantly low (p<0.05, at the confidence level of 95%), indicating a higher activity than the others.
Identification and quality of four varieties of adlay
Kesarin Lakkham,Preeya Wangsomnuk,Chantana Aromdee
Songklanakarin Journal of Science and Technology , 2009,
Abstract: Simple methods were used to identify and evaluate the quality of adlay, which is currently in focus for its nutraceuticalaspect and functional food. Four varieties of adlay, Coix lachryma-jobi L., Poaceae, were investigated for their physicochemical properties, DNA fingerprints, antioxidant activities, and the content of coixenolide. They were called waxy adlay(AD1), stone adlay (AD2), grey adlay (AD3), and normal adlay (AD4). Morphology of intact seeds of the four varieties can be distinguished visually. Iodine stained on starch granules of all varieties, except AD4, were blue. The gelling temperaturesranged from 67.92-73.27oC. TLC-fingerprints of acetone and methanol were documented. DNA fingerprints were established to differentiate the varieties. AD1 showed distinct properties over the others in the antioxidant activities, acetone extractive and coixenolide content.
Antihyperglycemic activity of agarwood leaf extracts in STZ-induced diabetic rats and glucose uptake enhancement activity in rat adipocytes
Ratree Pranakhon,Patchareewan Pannangpetch,Chantana Aromdee
Songklanakarin Journal of Science and Technology , 2011,
Abstract: Agarwood leaf extract was found to possess antipyretic, laxative and antimicrobial activities. A trial of one diabeticpatient taking long term agarwood leaf tea was found to have blood glucose returned to normal. In this work, the effects ofmethanol, water and hexane crude extracts of agarwood leaf on hyperglycemia in streptozotocin-induced diabetic rats wereinvestigated. Only methanol and water extracts at the dose of 1 g/kg body weight lowered the fasting blood glucose levels,54 and 40%, respectively. The results were comparable to 4 U/kg body weight of insulin (73%). In in vitro experiment, theeffect of the methanol and water extracts at the concentration of 10 g/mL enhanced glucose uptake activity on ratadipocytes by 172±10 and 176±21% of the control, respectively. The glucose uptake enhancement activity is comparable to1.5 nM insulin (166±16%). The thin-layer chromatographic characteristics of all extracts were documented. The findingssuggest that agarwood leaf is a promising potential antidiabetic agent.
High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens
Nithida Phonkot,Preeya Puangsomlee Wangsomnuk,Chantana Aromdee
Songklanakarin Journal of Science and Technology , 2010,
Abstract: A high-performance liquid chromatography method was developed to determine -carotene content in lotus stamens.The stamens of four varieties of Nelumbo nucifera (Gaertn.) and ten samples bought from traditional drug stores were determined.Validation of the method was carried out, the linearity of the -carotene concentrations range from 6.50-58.50 g.mL-1were 0.9997-0.9998. The repeatability and intermediate precision were 0.29 %CV (n=9) and 7.48 %CV (n=9), respectively; theaccuracy was 100.10 %w/w, the detection limit was 9.83 ng.mL-1 and the quantitation limit was 29.80 ng.mL-1. The -caroteneof the four varieties were 465.77-1150.80 mg% (n=3), and of the store samples were 4.70-41.73 mg% (n=3). The resultsdemonstrated that the contents varied according to the variety. The contents in the samples obtained from traditionaldrug stores were much lower that those dried in our laboratory, this might due to the source of the stamens, duration, andconditions of storages.
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