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Masas retroperitoneales en el período perinatal
Castagnaro Rosini,Nelda María; Castagnaro,María Carolina;
Revista argentina de radiolog?-a , 2011,
Abstract: purpose: to identify the most frequent causes of retroperitoneal mass during the fetal and neonatal period, and to illustrate the imaging findings based on our experience. material and methods: retrospective study about the etiology of retroperitoneal masses in newborns based on x-ray analysis, ultrasound and clinical findings of 487 infants who underwent abdominal ultrasound between january 2008 and december 2009. a total of 47 newborns with a diagnosis of retroperitoneal mass were selected. patients with prenatal ultrasound diagnosis of intraabdominal retroperitoneal mass and those with palpable abdominal mass on their physical examination in the neonatal period were included in this sample. infants with gastrointestinal and intraperitoneal abdominal pathology were excluded. results: of all the study patients (n= 47), 42 had renal masses (89%), and 5 retroperitoneal extrarenal masses (11%). among the former, the prevalent diseases were hydronephrosis in 36 cases (76%), and multicystic renal dysplasia in 4 (9%). moreover, there was only 1 case of congenital mesoblastic nephroma, and 1 case of autosomal recessive polycystic disease. among the latter, there were 3 infants with adrenal hemorrhage (6%), and 2 with neuroblastoma (4%). conclusion: the incidence of perinatal retroperitoneal masses in the studied population was 9.65%, in agreement with the literature. it highlights the importance of prenatal us to define the obstetric behavior and the coordination between pre- and postnatal ultrasound in order to achieve the best diagnostic approach. in our casuistic, a diagnosis was reached through us in 94% of the cases. ct plays a crucial role in both defining the surgical procedure in congenital mesoblastic nephroma and staging neuroblastomas.
Masas retroperitoneales en el período perinatal Retroperitoneal masses in the perinatal period
Nelda María Castagnaro Rosini,María Carolina Castagnaro
Revista Argentina de Radiología , 2011,
Abstract: Objetivo: identificar las causas más frecuentes de masa retroperitoneal durante el período fetal y neonatal e ilustrar los hallazgos imagenológicos basados en los resultados obtenidos de nuestra experiencia. Material y método: estudio retrospectivo observacional de las causas de masas retroperitoneales en recién nacidos (RN). Se analizaron historias clínicas y hallazgos radiológicos y ultrasonográficos de 487 neonatos a los que se les había realizado ecografía abdominal entre enero de 2008 y diciembre de 2009. Se seleccionaron 47 RN con diagnóstico de lesión retroperitoneal, respetando los siguientes criterios: Inclusión: pacientes con diagnóstico ecográfico prenatal de masa intraabdominal retroperitoneal y neonatos que presentaron al examen físico masa abdominal palpable y fueron estudiados con diferentes técnicas de diagnóstico por imagen. Exclusión: neonatos con patología abdominal gastrointestinal e intraperitoneal. Resultados: del total de pacientes (n =47), 42 presentaron masas de origen renal (89%) y 5 retroperitoneal extrarrenal (11%). Las patologías prevalentes fueron hidronefrosis con 36 casos (76%) y displasia renal multiquística con 4 (9%). Se registró un caso de nefroma mesoblástico congénito y 1 con enfermedad poliquística autosómica recesiva. Se detectaron 3 RN con hemorragia suprarrenal (6%) y 2 con neuroblastoma (4%). Conclusión: la incidencia de Masas Retroperitoneales en la población estudiada fue del 9,65%, coincidente con la bibliografía. El presente trabajo destaca el valor del US prenatal, importante para definir conducta obstétrica, y la coordinación entre la ecografía pre y posnatal para lograr una mayor aproximación al diagnóstico. El rol fundamental de la TC para definir conducta quirúrgica en el nefroma mesoblástico congénito y para estadificación en los casos de neuroblastoma. Purpose: To identify the most frequent causes of retroperitoneal mass during the fetal and neonatal period, and to illustrate the imaging findings based on our experience. Material and Methods: Retrospective study about the etiology of retroperitoneal masses in newborns based on x-ray analysis, ultrasound and clinical findings of 487 infants who underwent abdominal ultrasound between January 2008 and December 2009. A total of 47 newborns with a diagnosis of retroperitoneal mass were selected. Patients with prenatal ultrasound diagnosis of intraabdominal retroperitoneal mass and those with palpable abdominal mass on their physical examination in the neonatal period were included in this sample. Infants with gastrointestinal and intraperitoneal abdominal
Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods
Luciano A Rigano, María R Marano, Atilio P Castagnaro, Alexandre Do Amaral, Adrian A Vojnov
BMC Microbiology , 2010, DOI: 10.1186/1471-2180-10-176
Abstract: A loop-mediated isothermal amplification assay for the diagnosis of Citrus Bacterial Canker (CBC-LAMP) was developed and evaluated. DNA samples were obtained from infected plants or cultured bacteria. A typical ladder-like pattern on gel electrophoresis was observed in all positive samples in contrast to the negative controls. In addition, amplification products were detected by visual inspection using SYBRGreen and using a lateral flow dipstick, eliminating the need for gel electrophoresis. The sensitivity and specificity of the assay were evaluated in different conditions and using several sample sources which included purified DNA, bacterium culture and infected plant tissue. The sensitivity of the CBC-LAMP was 10 fg of pure Xcc DNA, 5 CFU in culture samples and 18 CFU in samples of infected plant tissue. No cross reaction was observed with DNA of other phytopathogenic bacteria. The assay was capable of detecting CBC-causing strains from several geographical origins and pathotypes.The CBC-LAMP technique is a simple, fast, sensitive and specific method for the diagnosis of Citrus Bacterial Canker. This method can be useful in the phytosanitary programs of the citrus industry worldwide.Citrus Bacterial Canker is an economic important disease in several countries, and causes great losses in fruit production and its subsidiaries [1]. There are three types of Citrus Bacterial Canker identified that have different genotypes and posses variations in host range among citrus plants. The type A CBC originating from Asia, is caused by Xanthomonas citri subsp. citri, this is the most destructive and widespread variant of the disease with a host range that includes all citrus cultivars [2]. The CBC types B and C are caused by Xanthomonas fuscans subsp. aurantifolii strains B and C, respectively. Those bacteria are limited in host range and are geographically restricted to South America. Type B CBC is present only in Argentina, Uruguay and Paraguay and is found primarily on le
En anticipación de una vacuna antirrotavirus: revisión de estudios epidemiológicos sobre la diarrea por rotavirus en la Argentina
Gómez Jorge A.,Nates Silvia,Castagnaro Nelda R. de,Espul Carlos
Revista Panamericana de Salud Pública , 1998,
Abstract: En todo el mundo, los rotavirus son la causa más común de diarrea grave en los ni os peque os y actualmente se están ensayando sobre el terreno vacunas que posiblemente permitan inmunizar a la población infantil dentro de varios a os. Con el fin de estimar la carga de enfermedad por rotavirus en la Argentina y la utilidad de establecer en el país un sistema de vigilancia de la enfermedad, se revisaron datos sobre la detección de estos virus, según estudios publicados y otros inéditos de nueve ciudades argentinas y uno multicéntrico. Los informes revisados indican que se detectaron rotavirus en 20% de 5 226 especímenes (con un recorrido de 6 a 54% entre estudios) tomados de ni os hospitalizados por diarrea y en 9% de 6 587 especímenes (recorrido de 5 a 22% entre estudios) tomados de pacientes ambulatorios, miembros de poblaciones mixtas (ni os hospitalizados y ambulatorios) y sujetos de encuestas comunitarias. Los datos hospitalarios muestran que, si bien los virus se detectaron durante todo el a o, en los meses de invierno (mayo a julio) hubo un pico de intensidad cuando hasta la mitad de los ni os con diarrea dieron resultados positivos a los rotavirus. En tres laboratorios se logró serotipificar para la proteína G 230 de 294 especímenes positivos (78%); los resultados indican que el serotipo G1 fue el más común (presente en 60% de los especímenes serotipificados) seguido del G2 (en 20%), G4 (en 14%) y G3 (en 5%). Sobre la base de los datos obtenidos en el país, se estimó que en 1991 en la Argentina hubo aproximadamente 84 500 visitas de pacientes ambulatorios (1 de cada 8 nacimientos) y 21 000 hospitalizaciones de 4 días en promedio (1 de cada 31 nacimientos) asociadas con rotavirus, que en conjunto costaron unos US$27,7 millones. Estos datos preliminares muestran que la carga de enfermedad por rotavirus en los ni os argentinos es muy pesada y podría disminuirse con una vacuna segura y efectiva. Además se necesita ampliar la vigilancia para mejorar el conocimiento de la epidemiología y de la distribución de las cepas de rotavirus en el país, calcular más exactamente la eficacia en función del costo de un programa de vacunación antirrotavirus y determinar los mejores métodos de monitorear sus efectos.
Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina
Gómez Jorge A.,Nates Silvia,De Castagnaro Nelda R.,Espul Carlos
Revista Panamericana de Salud Pública , 1998,
Abstract: Rotavirus is the most common cause of severe diarrhea in children worldwide, and vaccines currently being field-tested could be available for childhood immunization in several years. To assess the rotavirus disease burden in Argentina and the value of future national surveillance for the disease, we reviewed available data on rotavirus detections reported by published and unpublished studies conducted in nine Argentine cities and by a multicenter study. Data from these studies indicated that rotavirus was detected in 20% of 5 226 specimens (within a range of 6% to 54% for different studies) from children hospitalized for diarrhea and in 9% of 6 587 specimens (within a range of 5% to 22% for different studies) from children who were outpatients, members of mixed populations (hospitalized patients and outpatients), or survey subjects in community-based studies. The hospital data showed that while rotavirus was detected throughout the year, a peak occurred during the winter months (May-July), when up to half of the children with diarrhea were found positive for rotavirus. Attempted serotyping of 294 rotavirus-positive specimens for G-protein by three laboratories was successful in 230 cases (78%); the resulting data indicated that serotype G1 was the most common (being present in 60% of the successfully serotyped specimens), followed by G2 (in 20%), G4 (in 14%), and G3 (in 5%). Based on national data for Argentina, we estimate that in 1991 there were roughly 84 500 rotavirus-associated outpatient visits (1 for every 8 births) and 21 000 hospitalizations averaging 4 days in length (1 for every 31 births), all of which entailed direct medical costs estimated at US$ 27.7 million. These preliminary data show that the rotavirus disease burden in Argentine children is extensive and could be decreased by a safe and effective vaccine. Further surveillance is needed to improve our understanding of the epidemiology and distribution of rotavirus strains in Argentina, to more accurately assess the cost-effectiveness of a rotavirus vaccine program, and to indicate what methods might best be used to monitor such a program's impact.
Transcriptomic markers meet the real world: finding diagnostic signatures of corticosteroid treatment in commercial beef samples
Sara Pegolo, Guglielmo Gallina, Clara Montesissa, Francesca Capolongo, Serena Ferraresso, Caterina Pellizzari, Lisa Poppi, Massimo Castagnaro, Luca Bargelloni
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-205
Abstract: Quantitative determination of Dexamethasone (DEX) residues in the urine collected at the slaughterhouse was performed by Liquid Chromatography-Mass Spectrometry (LC-MS). DNA-microarray technology was used to obtain transcriptomic profiles of skeletal muscle in commercial samples and negative controls. LC-MS confirmed the presence of low level of DEX residues in the urine of the commercial samples suspect for histological classification. Principal Component Analysis (PCA) on microarray data identified two clusters of samples. One cluster included negative controls and a subset of commercial samples, while a second cluster included part of the specimens collected at the slaughterhouse together with positives for corticosteroid treatment based on thymus histology and LC-MS. Functional analysis of the differentially expressed genes (3961) between the two groups provided further evidence that animals clustering with positive samples might have been treated with corticosteroids. These suspect samples could be reliably classified with a specific classification tool (Prediction Analysis of Microarray) using just two genes.Despite broad variation observed in gene expression profiles, the present study showed that DNA-microarrays can be used to find transcriptomic signatures of putative anabolic treatments and that gene expression markers could represent a useful screening tool.The use of growth promoters in meat production has been banned in the European Union since 1988 due to the potentially adverse effects of hormone residues for the consumer. Council Directive 23/96/EC requires the EU member States to adopt National Monitoring Plans to control the illegal use of these compounds. Despite the ban, these substances are still administered and a black-market for the production, distribution, and use of multiple steroids has flourished [1]. To elude official controls, new anabolic compounds are developed and growth promoters are administered at low doses or combining different
Radiografía directa de abdomen y correlación clínica en la enterocolitis necrotizante
Castagnaro,María Carolina; López Ma?án,Julio; Ada Miriam,Góngora; Nishihara Hun,Mey Ling;
Revista argentina de radiolog?-a , 2009,
Abstract: purpose: determine the incidence of necrotizing enterocolitis (nec) on the studied population and categorized the radiological signs based on the bell clinical - radiological classification, in 6 grades from lower to higher severity (ia and b, iia and b and iiia and b), necessary to define prognosis and therapeutical behaviors. materials and method: retrospective analysis of 550 clinical cases of neonates younger than 36 weeks assisted in uci neonatal between april and september 2003. fourty five new born that developed nec were selected, and the gestational age (ga), weight and clinical radiological presentation were evaluated. results: from the 550 nb, 45 (8%) showed nec. the ga that more frequently appeared was in between 31 and 34 weeks (60%) and 71% of the new born's weight was in between 1000 and 2000 grams. the clinical and radiological presentation by the bell classification was: ia: 9 cases (20%); ib: 10 cases (22%); iia: 11 cases (24%); iib: 7 cases (15%); iiia: 3 cases (7%); iiib: 5 cases (11%). conclusion: the incidence of nec in the studied population was 8%, which agrees with the bibliography. it is confirmed the value of direct abdominal radiography, its role is not only linked to the diagnostic of the diseases, but it also gives valuable elements for the prognosis, evolutionary control and detection of complications.
Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina
Gómez,Jorge A.; Nates,Silvia; De Castagnaro,Nelda R.; Espul,Carlos; Borsa,Ana; Glass,Roger I.;
Revista Panamericana de Salud Pública , 1998, DOI: 10.1590/S1020-49891998000200001
Abstract: rotavirus is the most common cause of severe diarrhea in children worldwide, and vaccines currently being field-tested could be available for childhood immunization in several years. to assess the rotavirus disease burden in argentina and the value of future national surveillance for the disease, we reviewed available data on rotavirus detections reported by published and unpublished studies conducted in nine argentine cities and by a multicenter study. data from these studies indicated that rotavirus was detected in 20% of 5 226 specimens (within a range of 6% to 54% for different studies) from children hospitalized for diarrhea and in 9% of 6 587 specimens (within a range of 5% to 22% for different studies) from children who were outpatients, members of mixed populations (hospitalized patients and outpatients), or survey subjects in community-based studies. the hospital data showed that while rotavirus was detected throughout the year, a peak occurred during the winter months (may-july), when up to half of the children with diarrhea were found positive for rotavirus. attempted serotyping of 294 rotavirus-positive specimens for g-protein by three laboratories was successful in 230 cases (78%); the resulting data indicated that serotype g1 was the most common (being present in 60% of the successfully serotyped specimens), followed by g2 (in 20%), g4 (in 14%), and g3 (in 5%). based on national data for argentina, we estimate that in 1991 there were roughly 84 500 rotavirus-associated outpatient visits (1 for every 8 births) and 21 000 hospitalizations averaging 4 days in length (1 for every 31 births), all of which entailed direct medical costs estimated at us$ 27.7 million. these preliminary data show that the rotavirus disease burden in argentine children is extensive and could be decreased by a safe and effective vaccine. further surveillance is needed to improve our understanding of the epidemiology and distribution of rotavirus strains in argentina, to more accu
En anticipación de una vacuna antirrotavirus: revisión de estudios epidemiológicos sobre la diarrea por rotavirus en la Argentina
Gómez,Jorge A.; Nates,Silvia; Castagnaro,Nelda R. de; Espul,Carlos; Borsa,Ana; Glass,Roger I.;
Revista Panamericana de Salud Pública , 1998, DOI: 10.1590/S1020-49891998000600003
Abstract: rotavirus is the most common cause of severe diarrhea in children worldwide, and vaccines currently being field-tested could be available for childhood immunization in several years. to assess the rotavirus disease burden in argentina and the value of future national surveillance for the disease, we reviewed available data on rotavirus detections reported by published and unpublished studies conducted in nine argentine cities and by a multicenter study. data from these studies indicated that rotavirus was detected in 20% of 5 226 specimens (within a range of 6% to 54% for different studies) from children hospitalized for diarrhea and in 9% of 6 587 specimens (within a range of 5% to 22% for different studies) from children who were outpatients, members of mixed populations (hospitalized patients and outpatients), or survey subjects in community-based studies. the hospital data showed that while rotavirus was detected throughout the year, a peak occurred during the winter months (may-july), when up to half of the children with diarrhea were found positive for rotavirus. attempted serotyping of 294 rotavirus-positive specimens for g-protein by three laboratories was successful in 230 cases (78%); the resulting data indicated that serotype g1 was the most common (being present in 60% of the successfully serotyped specimens), followed by g2 (in 20%), g4 (in 14%), and g3 (in 5%). based on national data for argentina, we estimate that in 1991 there were roughly 84 500 rotavirus-associated outpatient visits (1 for every 8 births) and 21 000 hospitalizations averaging 4 days in length (1 for every 31 births), all of which entailed direct medical costs estimated at us$ 27.7 million. these preliminary data show that the rotavirus disease burden in argentine children is extensive and could be decreased by a safe and effective vaccine. further surveillance is needed to improve our understanding of the epidemiology and distribution of rotavirus strains in argentina, to more accu
Diversidad genética del complejo de virosis asociadas a la enfermedad del mosaico de la ca?a de azúcar en Tucumán, Argentina
Perera,María F.; Filippone,María P.; Ramallo,Jacqueline C.; Cuenya,María I.; Castagnaro,Atilio P.;
Revista industrial y agr?-cola de Tucum??n , 2009,
Abstract: sugarcane mosaic is one of the most important systemic diseases of sugarcane. sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) are the causal agents of the disease. sugarcane leaves with mosaic symptoms were analysed by rt-pcr-rflp (reverse transcriptase-polymerase chain reaction- restriction fragment length polymorphism) and the nucleotide sequences of the coat protein (cp) genes amplified to assess the presence and genetic diversity of both viruses in the sugarcane producing area in tucumán. using the primers scmvr3/f4, 93% of samples were scmv positive and 33% of them had the e strain rflp profile, while the rest produced nine different profiles that did not match those of any known strains. sequence analyses showed that 20% of the samples with the unknown profiles were highly identical to the scmv d strain, while the rest differed significantly from each other. the presence of the flexuous virions typical of potyviruses was confirmed by transmission electron microscopy. also, using the specific primers, the presence of srmv was detected in 90% of the samples, and co-existence of both viruses was found in 85% of the samples. rflp analysis determined the presence of srmv strains m and i in 68% and 14% of the samples, respectively, while in approximately 18% of the cases, both m and h strains were present. no rt-pcr product was produced by either scmv or srmv primer pairs in one symptomatic sample, suggesting the presence of another pathogen producing similar symptomatology.
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