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Search Results: 1 - 10 of 842004 matches for " Carmem Lúcia M. S. C. Rocha "
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Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses
Fábio O. Pedrosa ,Rose Adele Monteiro,Roseli Wassem,Leonardo M. Cruz,Ricardo A. Ayub,Nelson B. Colauto,Maria Aparecida Fernandez,Maria Helena P. Fungaro,Edmundo C. Grisard,Mariangela Hungria,Humberto M. F. Madeira,Rubens O. Nodari,Clarice A. Osaku,Maria Luiza Petzl-Erler,Hernán Terenzi,Luiz G. E. Vieira,Maria Berenice R. Steffens,Vinicius A. Weiss,Luiz F. P. Pereira,Marina I. M. Almeida,Lysangela R. Alves,Anelis Marin,Luiza Maria Araujo,Eduardo Balsanelli,Valter A. Baura,Leda S. Chubatsu,Helisson Faoro,Augusto Favetti,Geraldo Friedermann,Chirlei Glienke,Susan Karp,Vanessa Kava-Cordeiro,Roberto T. Raittz,Humberto J. O. Ramos,Enilze Maria S. F. Ribeiro,Liu Un Rigo,Saul N. Rocha,Stefan Schwab,Anilda G. Silva,Eliel M. Souza,Michelle Z. Tadra-Sfeir,Rodrigo A. Torres,Audrei N. G. Dabul,Maria Albertina M. Soares,Luciano S. Gasques,Ciela C. T. Gimenes,Juliana S. Valle,Ricardo R. Ciferri,Luiz C. Correa,Norma K. Murace,Jo?o A. Pamphile,Eliana Valéria Patussi,Alberto J. Prioli,Sonia Maria A. Prioli,Carmem Lúcia M. S. C. Rocha,Olívia Márcia N. Arantes,Márcia Cristina Furlaneto,Leandro P. Godoy,Carlos E. C. Oliveira,Daniele Satori,Laurival A. Vilas-Boas,Maria Angélica E. Watanabe,Bibiana Paula Dambros,Miguel P. Guerra,Sandra Marisa Mathioni,Karine Louise Santos,Mario Steindel,Javier Vernal,Fernando G. Barcellos,Rubens J. Campo,Ligia Maria O. Chueire,Marisa Fabiana Nicolás,Lilian Pereira-Ferrari,José L. da Concei??o Silva,Nereida M. R. Gioppo,Vladimir P. Margarido
PLOS Genetics , 2011, DOI: 10.1371/journal.pgen.1002064
Abstract: The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species.
Determina??o de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detec??o por arranjo de diodos
Toscano, Ilda A. S.;Ribeiro, M. Lúcia;Rocha, Julio C.;Nunes, Gilvanda S.;Barceló, Damiá;
Química Nova , 2000, DOI: 10.1590/S0100-40422000000400007
Abstract: elisas have been applied to pesticide residue analysis due to their high sensitivity and selectivity. however, some elisas performance may be affected by matrix components. in this work, elisa for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. the results suggested that the high fulvic acid concentration (?330 mg l-1) and acidic ph conditions (ph 4.0) interfere with the signal detection decreasing the method sensitivity. a dilution of the samples and adjust to ph 8.0 are appropriate to minimize the matrix interferences in the elisa method. good correlation between elisa and hplc-dad results was observed.
Determina o de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detec o por arranjo de diodos
Toscano Ilda A. S.,Ribeiro M. Lúcia,Rocha Julio C.,Nunes Gilvanda S.
Química Nova , 2000,
Abstract: ELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration ( superscript three30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.
Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
Pamphile, Jo?o A.;Rocha, Carmen Lúcia M.S.C. da;Azevedo, Jo?o L.;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000200021
Abstract: a tropical endophytic isolate of the fungus fusarium verticillioides (synonym fusarium moniliforme) obtained from zea mays was co-transformed with plasmid pnh24 containing the fusarium oxysporum nitrate reductase nia gene and plasmid pnom 102 carrying the escherichia coli b-glucuronidase gusa gene. transformation frequency for the nia marker was 75 transformants mg-1 vector dna and introduction of the gusa gene by co-transformation was 57.2% as indicated by the presence of the gus+ phenotype on plates. southern analyses confirmed the integration of both plasmids into the genome of ten gus+ transformants. all co-transformants showed mitotic stability in respect of the gus+ phenotype. to assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected gus+ phenotype?.
Constituintes químicos e avalia??o preliminar in vivo da atividade antimalárica de Ouratea nitida Aubl (Ochnaceae)
Estevam, Charles S.;Oliveira, Fernando M.;Conserva, Lúcia M.;Lima, Lúcia de Fátima C.O.;Barros, Elaine C.P.;Barros, ?ngela C.P.;Rocha, Eliana M.M.;Andrade, Eloísa H.A.;
Revista Brasileira de Farmacognosia , 2005, DOI: 10.1590/S0102-695X2005000300005
Abstract: this work describes the isolation of triterpenes (3,4-seco-friedelan-3-oic acid, friedelin, and b-amyrin) and other compounds (p-hydroxybenzoic acid ethyl ester and tetracosane) as well as a preliminary evaluation of in vivo antimalarial activity of the extracts from the leaves of ouratea nitida aubl. qualitative analysis by gc-ms of an apolar fraction from the hexane extract was also carried out. methyl and ethyl esters of lauric, myristic, palmitic, stearic and oleic acids, methyl ester of pentadecanoic, heptadecanoic, arachidonic, beenic and lignoceric acids, and ethyl ester of linoleic acid were found to be the main constituents while n-butyl stearate, tetracosane and 6,10,14-trimetthyl-2-pentadecanone were the minor. all isolated compounds were identified on basis of the spectral data (ir, ms and nmr, including dept) and are being described for the first time in this specie.
The influence of HCV coinfection on clinical, immunological and virological responses to HAART in HIV-patients
Carmo, Ricardo A.;Guimar?es, Mark D.C.;Moura, Alexandre S.;Neiva, Augusto M.;Versiani, Juliana B.;Lima, Letícia V.;Freitas, Lílian P.;Rocha, Manoel Otávio C.;
Brazilian Journal of Infectious Diseases , 2008, DOI: 10.1590/S1413-86702008000300003
Abstract: the potential impact of the hepatitis c virus (hcv) on clinical, immunological and virological responses to initial highly active antiretroviral therapy (haart) of patients infected with human immunodeficiency virus (hiv) is important to evaluate due to the high prevalence of hiv-hcv coinfection. a historical cohort study was conducted among 824 hiv-infected patients starting haart at a public referral service in belo horizonte, brazil, to assess the impact of hcv seropositivity on appearance of a new aids-defining opportunistic illness, aids-related death, suppression of viral load, and an increase in cd4-cell count. a total of 76 patients (9.2%) had a positive hcv test, 26 of whom (34.2%) had a history of intravenous drug use. in multivariate analysis, hcv seropositivity was associated with a smaller cd4-cell recovery (rh=0.68; 95% ci [0.49-0.92], but not with progression to a new aids-defining opportunistic illness or to aids-related death (rh=1.08; 95% ci [0.66-1.77]), nor to suppression of hiv-1 viral load (rh=0.81; 95% ci [0.56-1.17]) after starting haart. these results indicate that although associated with a blunted cd4-cell recovery, hcv coinfection did not affect the morbidity or mortality related to aids or the virological response to initial haart.
Pseudoaneurisma de ventrículo esquerdo associado a insuficiência mitral grave complicando infarto agudo do miocárdio ínfero-látero-dorsal
Falc?o, Jo?o Luiz de A. A.;Falc?o, Sandra Nívea R. S.;Garcia, Maria Fernanda M. A.;Arruda, Ana Lúcia M.;Hueb, Alexadre C.;Jatene, Fábio B.;Gutierrez, Paulo S.;Nicolau, José Carlos;Ramires, José Ant?nio F.;Giraldez, Roberto Rocha C. V.;
Arquivos Brasileiros de Cardiologia , 2005, DOI: 10.1590/S0066-782X2005000600011
Abstract: we described a case of left ventricular pseudoaneurysm associated to a severe mitral regurgitation, complicating a inferolaterodorsal acute myocardial infarction. the lesion was found in a routine echocardiogram during the in-hospital follow-up. the well-succeeded surgical strategy and the good clinical evolution of the patient were distinguished.
Leucemia Mielóide Agura: perfil de duas décadas do Servi?o de Hematologia do Hospital das Clínicas de Porto Alegre - RS
Bittencourt, Rosane;Fogliato, Laura;Daudt, Liane;Bittencourt, Henrique N. S.;Friederich, Jo?o R.;Fernandes, Flavo;Onsten, Tor;Fassina, Kátia;Rocha, Luis K. da;Moreno, Fábio;Silva, Geórgia;Cruz, Matheus S.;Garcia, Régis G. de;Wasnievski, Jo?o C.;Silla, Lúcia M. R.;
Revista Brasileira de Hematologia e Hemoterapia , 2003, DOI: 10.1590/S1516-84842003000100004
Abstract: we have treated many aml patients in the last 20 years. we followed, between march 1980 and december 1999, 195 patients with ages ranging from 12 to 70 years and presenting "de novo" aml, excluding the m3 subtype. in the eighties, 102 patients are on record: 47 males and 55 females. among these, 84 received induction chemotherapy with cytarabin plus daunorrubicin (7+3), resulting in a 51% (43/84) remission rate. the average disease-free and overall survival was 35% at 10 months and 13% at 12 months. forty-one new diagnoses were performed from january 1990 and december 1993, all patients were submitted to the "7+3" chemotherapy protocol and a 66% remission rate was obtained. the disease free and overall survival rates were statistically (p < 0.001) higher with 41% of the patients at 7.6 months and 17.5% at 22 months. in 1994 a new protocol was introduced under the guidance of the brazilian cooperative group on leukemia studies (gcbel). it recommended induction with cytarabin + idarrubicin followed by consolidation and two intensification cycles with high doses of cytarabin. between 1994 and 1999, 52 patients were included in this new protocol and the remission rate was 73% (p = 0.002). in 50% of these patients disease free survival was about 23.3 months, while the overall survival was 25% at 26 months. both disease free survival and overall survival rates in the last period showed statistically significant differences when compared to the previous years. we observed a progressive improvement in remission and survival rates in the last decades, however the prolonged event-free survival stayed below 30%. our results are similar to the national centers that treat aml and in accordance with the literature data.
Forma cística da sialometaplasia necrosante em glandula salivar sublingual
Lima Marcus A.,Rocha Letícia C.,Siqueira Luciana M. S.,Carmo Luís C.
Revista Brasileira de Otorrinolaringologia , 2002,
Abstract: A sialometaplasia necrosante (SN) é doen a inflamatória de natureza benigna que se manifesta como úlceras profundas e irregulares de crescimento rápido, acometendo glandulas salivares menores, simulando neoplasias malignas. Relata-se caso de SN que se destaca pela forma de apresenta o cística em assoalho da boca de mulher de 70 anos, com boa evolu o pós-operatória e sem evidências de recidiva um ano após a ressec o.
ß-Globin Gene Cluster Haplotypes and Clinical Severity in Sickle Cell Anemia Patients in Southern Brazil  [PDF]
Maria A. L. da Silva, Jo?o R. Friedrisch, Christina M. Bittar, Meide Urnau, Jóice Merzoni, Vanessa S. Valim, Bruna Amorin, Annelise Pezzi, José Artur B. Chies, Lúcia M. da Rocha Silla
Open Journal of Blood Diseases (OJBD) , 2014, DOI: 10.4236/ojbd.2014.42003
Hematopoietic stem cell transplantation(HSCT)has emerged as a curative strategy for sickle cell anemia(SCA); it is necessary to find markers of SCA clinical severity to spare those SCA patients whose clinical course is mild from the morbidity and mortality associated with HSCT. Haplotypes have been correlated with the severity of clinical manifestations in SCA patients, and fetal hemoglobin(HbF)and socioeconomic status(SeS)have also been described as negative factors. We studied these factors and their impact on clinical manifestations in a population of Southern Brazilian patients attending the Center for Sickle Cell Anemia at Hospital de Clínicas de Porto Alegre/RS, Brazil. Clinical severity was defined as two or more veno-occlusive episodes per year. The βS haplotypes were determined by PCR in 75 SCA patients. Among the 150 βS chromosomes analyzed, 99(66%)were identified as Bantu(Ban), 41(27%)asBenin(Ben), and 10(7%)as other haplotypes. Most patients in our sample(62.7%)belonged to lower SeS groups, precluding meaningful statistical analysis of SeS impact on clinical severity. There was no correlation between haplotypes or HbF level and SCA clinical severity. Gene polymorphisms and environmental issues have to be taken into consideration.
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