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Search Results: 1 - 10 of 51395 matches for " Carlos Leomar Zani "
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Eleutherinone, a novel fungitoxic naphthoquinone from Eleutherine bulbosa (Iridaceae)
Alves, Tania Maria Almeida;Kloos, Helmut;Zani, Carlos Leomar;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000500021
Abstract: the dichloromethane extract prepared from the underground parts of eleutherine bulbosa (miller) urban (iridaceae) showed strong activity in the direct bioautography assay with the phytopathogenic fungus cladosporium sphaerospermum. this assay was used to guide the fractionation of this extract and allowed the isolation of four compounds: the new naphthoquinone eleutherinone[8-methoxy-1-methyl-1,3-dihydro-naphtho(2,3-c)furan-4,9 -dione] and the known compounds, previously isolated from this species, eleutherin [9-methoxy-1(r),3(s)-dimethyl-3,4-dihydro-1h-benzo(g)isochromene-5,10-dione], isoeleutherin [9-methoxy-1(r),3(r)-dimethyl-3,4-dihydro-1h-benzo(g)isochromene-5,10-dione], and eleutherol [4-hydroxy-5-methoxy-3(r)-methyl-3h-naphtho(2,3-c)furan-1 -one]. all quinonoid compounds showed strong antifungal activity in the bioautography assay at 100 μg/spot, while eleutherol was inactive.
Eleutherinone, a novel fungitoxic naphthoquinone from Eleutherine bulbosa (Iridaceae)
Alves Tania Maria Almeida,Kloos Helmut,Zani Carlos Leomar
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: The dichloromethane extract prepared from the underground parts of Eleutherine bulbosa (Miller) Urban (Iridaceae) showed strong activity in the direct bioautography assay with the phytopathogenic fungus Cladosporium sphaerospermum. This assay was used to guide the fractionation of this extract and allowed the isolation of four compounds: the new naphthoquinone eleutherinone[8-methoxy-1-methyl-1,3-dihydro-naphtho(2,3-c)furan-4,9 -dione] and the known compounds, previously isolated from this species, eleutherin [9-methoxy-1(R),3(S)-dimethyl-3,4-dihydro-1H-benzo(g)isochromene-5,10-dione], isoeleutherin [9-methoxy-1(R),3(R)-dimethyl-3,4-dihydro-1H-benzo(g)isochromene-5,10-dione], and eleutherol [4-hydroxy-5-methoxy-3(R)-methyl-3H-naphtho(2,3-c)furan-1 -one]. All quinonoid compounds showed strong antifungal activity in the bioautography assay at 100 μg/spot, while eleutherol was inactive.
Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum
Alves, Tania Maria de Almeida;Ribeiro, Fabiane Lacerda;Kloos, Helmut;Zani, Carlos Leomar;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000600016
Abstract: polygonum punctatum (polygonaceae) is an herb known in some regions of brazil as "erva-de-bicho" and is used to treat intestinal disorders. the dichloromethane extract of the aerial parts of this plant showed strong activity in a bioautographic assay with the fungus cladosporium sphaerospermum. the bioassay-guided chemical fractionation of this extract afforded the sesquiterpene dialdehyde polygodial as the active constituent. the presence of this compound with antibiotic, anti-inflammatory and anti-hyperalgesic properties in "erva-de-bicho" may account for the effects attributed by folk medicine to this plant species.
Ensaios preliminares do Guaiacum officinale L. como moluscicida
Mendes, Nelymar Martineli;Gómez, José D.;Araújo, Neusa;Zani, Carlos Leomar;Katz, Naftale;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1993, DOI: 10.1590/S0036-46651993000600006
Abstract: aqueous suspensions of fruit's pericarp, leaves, root's bark and seeds of guaiacum officinale were tested at different concentrations as molluscicide, cercaricide and piscicide. in the laboratory the suspension of fruit's pericarp produced 100% mortality for egg masses of b. glabrata at 100 ppm, for adult snails of biomphalaria glabrata, b. straminea and b. tenagophila at 20 ppm, for lebistes reticulatus (fishes) at 5 ppm and schistosoma mansoni's cercariae at 1 ppm. the ethanolic extract of fruit's pericarp was not active against adult snails of b. glabrata. the letal dose for adult snails(dl90) of the aqueous suspension of fruit's pericarp after 24 hours exposure, were: 15 ppm for b. glabrata; 14 ppm for b. straminea and 18 ppm for b. tenagophila. the dl90 of aqueous suspensions of root's bark, seeds and leaves were 57 ppm, 33 ppm and 15 ppm, respectively. in the field, b. glabrata adult snail mortality was 68% at 20 ppm and 100% at 40 ppm, when using suspension of fruit's pericarp
Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum
Alves Tania Maria de Almeida,Ribeiro Fabiane Lacerda,Kloos Helmut,Zani Carlos Leomar
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: Polygonum punctatum (Polygonaceae) is an herb known in some regions of Brazil as "erva-de-bicho" and is used to treat intestinal disorders. The dichloromethane extract of the aerial parts of this plant showed strong activity in a bioautographic assay with the fungus Cladosporium sphaerospermum. The bioassay-guided chemical fractionation of this extract afforded the sesquiterpene dialdehyde polygodial as the active constituent. The presence of this compound with antibiotic, anti-inflammatory and anti-hyperalgesic properties in "erva-de-bicho" may account for the effects attributed by folk medicine to this plant species.
Screening of Brazilian basidiomycetes for antimicrobial activity
Rosa, Luiz Henrique;Machado, Kátia M Gomes;Jacob, Camila Cristina;Capelari, Marina;Rosa, Carlos Augusto;Zani, Carlos Leomar;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000700019
Abstract: a total of 103 isolates of basidiomycetes, representing 84 species from different brazilian ecosystems, were evaluated for their antifungal and antibacterial activity in a panel of pathogenic and non-pathogenic microorganisms. tissue plugs of the fruiting bodies were cultivated in liquid media and the whole culture extracted with ethyl acetate. crude extracts from agaricus cf. nigrecentulus, agrocybe perfecta, climacodon pulcherrimus, gloeoporus thelephoroides, hexagonia hydnoides, irpex lacteus, leucoagaricus cf. cinereus, marasmius cf. bellus, marasmius sp., nothopanus hygrophanus, oudemansiella canarii, pycnoporus sanguineus, phellinus sp., and tyromyces duracinus presented significant activity against one or more of the target microorganisms. eight isolates were active only against bacteria while three inhibited exclusively the growth of fungi. two extracts presented wide antimicrobial spectrum and were active against both fungi and bacteria. differences in the bioactivity of extracts obtained from isolates from the same species were observed.
Leishmanicidal Metabolites from Cochliobolus sp., an Endophytic Fungus Isolated from Piptadenia adiantoides (Fabaceae)
Fernanda Fraga Campos,Luiz Henrique Rosa,Betania Barros Cota,Rachel Basques Caligiorne,Ana Lúcia Teles Rabello,Tania Maria Almeida Alves,Carlos Augusto Rosa,Carlos Leomar Zani
PLOS Neglected Tropical Diseases , 2008, DOI: 10.1371/journal.pntd.0000348
Abstract: Protozoan parasites belonging to genera Leishmania and Trypanosoma are the etiological agents of severe neglected tropical diseases (NTDs) that cause enormous social and economic impact in many countries of tropical and sub-tropical areas of the world. In our screening program for new drug leads from natural sources, we found that the crude extract of the endophytic fungus Cochliobolus sp. (UFMGCB-555) could kill 90% of the amastigote-like forms of Leishmania amazonensis and inhibit by 100% Ellman's reagent reduction in the trypanothione reductase (TryR) assay, when tested at 20 μg mL?1. UFMGCB-555 was isolated from the plant Piptadenia adiantoides J.F. Macbr (Fabaceae) and identified based on the sequence of the internally transcribed spacer (ITS) regions of its ribosomal DNA. The chromatographic fractionation of the extract was guided by the TryR assay and resulted in the isolation of cochlioquinone A and isocochlioquinone A. Both compounds were active in the assay with L. amazonensis, disclosing EC50 values (effective concentrations required to kill 50% of the parasite) of 1.7 μM (95% confidence interval = 1.6 to 1.9 μM) and 4.1 μM (95% confidence interval = 3.6 to 4.7 μM), respectively. These compounds were not active against three human cancer cell lines (MCF-7, TK-10, and UACC-62), indicating some degree of selectivity towards the parasites. These results suggest that cochlioquinones are attractive lead compounds that deserve further investigation aiming at developing new drugs to treat leishmaniasis. The findings also reinforce the role of endophytic fungi as an important source of compounds with potential to enter the pipeline for drug development against NTDs.
A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
Cota, Betania Barros;Rosa, Luiz Henrique;Fagundes, Elaine Maria Souza;Martins-Filho, Olindo Assis;Correa-Oliveira, Rodrigo;Romanha, Alvaro José;Rosa, Carlos Augusto;Zani, Carlos Leomar;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000300007
Abstract: the fungus lentinus strigosus (pegler 1983) (polyporaceae, basidiomycete) was selected in a screen for inhibitory activity on trypanosoma cruzi trypanothione reductase (tr). the crude extract of l. strigosus was able to completely inhibit tr at 20 μg/ml. two triquinane sesquiterpenoids (dihydrohypnophilin and hypnophilin), in addition to two panepoxydol derivatives (neopanepoxydol and panepoxydone), were isolated using a bioassay-guided fractionation protocol. hypnophilin and panepoxydone displayed ic50 values of 0.8 and 38.9 μm in the tr assay, respectively, while the other two compounds were inactive. the activity of hypnophilin was confirmed in a secondary assay with the intracellular amastigote forms of t. cruzi, in which it presented an ic50 value of 2.5 μ m. quantitative flow cytometry experiments demonstrated that hypnophilin at 4 μm also reduced the proliferation of human peripheral blood monocluear cells (pbmc) stimulated with phytohemaglutinin, without any apparent interference on the viability of lymphocytes and monocytes. as the host immune response plays a pivotal role in the adverse events triggered by antigen release during treatment with trypanocidal drugs, the ability of hypnophilin to kill the intracellular forms of t. cruzi while modulating human pbmc proliferation suggests that this terpenoid may be a promising prototype for the development of new chemotherapeutical agents for chagas disease.
Comparison between two selected Saccharomyces cerevisiae strains as fermentation starters in the production of traditional cacha?a
Gomes, Fátima de Cássia Oliveira;Araújo, Roberta Amália de Carvalho;Cisalpino, Patrícia Silva;Moreira, Elizabeth Spangler Andrade;Zani, Carlos Leomar;Rosa, Carlos Augusto;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000200023
Abstract: two saccharomyces cerevisiae strains were tested as the starter yeasts in a traditional cacha?a distillery. the strains used were s. cerevisiae ufmg-a829, isolated from a cacha?a fermentation process, and s. cerevisiae k1-v1116, obtained from the wine industry. the permanence of each strain in the fermentation must was determined by rapd (random amplified polymorphic dna)-pcr, with primer m13. both yeast strains were prevalent in the vats for approximately 30 days. indigenous non-saccharomyces and indigenous s. cerevisiae strains were isolated in lower counts during the fermentation period. indigenous s. cerevisiae strains were molecularly distinct when compared to the starter yeasts. the two yeasts appeared promising starter yeasts in the fermentation process to produce traditional cacha?a.
Synthesis and in Vitro Cytotoxic Activity of Compounds with Pro-Apoptotic Potential
Gisell Apicela Soares,Renata Barbosa de Oliveira,Saulo Fernandes de Andrade,Ricardo José Alves,Carlos Leomar Zani,Elaine Maria De Souza-Fagundes
Molecules , 2010, DOI: 10.3390/molecules15010012
Abstract: In our search for new anticancer therapies, some compounds synthesized in our lab were selected and their potential cytotoxic activity was evaluated in vitro against two cancer cells lines including a solid tumor (UACC-62, melanoma) and a human lymphoma (JURKAT). Compounds showing cytotoxic activity were subjected to an apoptosis assay. Two compounds showed promising results.
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